JP2004523238A5 - - Google Patents

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JP2004523238A5
JP2004523238A5 JP2002566667A JP2002566667A JP2004523238A5 JP 2004523238 A5 JP2004523238 A5 JP 2004523238A5 JP 2002566667 A JP2002566667 A JP 2002566667A JP 2002566667 A JP2002566667 A JP 2002566667A JP 2004523238 A5 JP2004523238 A5 JP 2004523238A5
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JP
Japan
Prior art keywords
rna
seconds
template
serum
cepheid
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JP2002566667A
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JP4399164B2 (ja
JP2004523238A (ja
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Priority claimed from PCT/US2001/031637 external-priority patent/WO2002066993A1/en
Publication of JP2004523238A publication Critical patent/JP2004523238A/ja
Publication of JP2004523238A5 publication Critical patent/JP2004523238A5/ja
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Publication of JP4399164B2 publication Critical patent/JP4399164B2/ja
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Description

実施例16
磁化可能なセルロースを使用する血清からのRNA単離
MS2ウイルスRNA（1×107−1×108コピー）を3つの異なる血清試料に感染（spike）させた。次いで、各試料中のRNAを、実施例3と同様に単離した。次いで、精製したRNAを、以下の鋳型を使用してMS2 RT-PCRアッセイ法によって定量した。
Figure 2004523238
＊全体の反応容量が25μlである限りは、水とMS2 RNA鋳型のいかなる組み合わせも使用され得る。
以下の条件を用いてスマートサイクラー（Smart Cycler）（Cepheid、Sunnyvale、CA）中で反応混合物をサイクルに供した：60℃で30分間、次に95℃で120秒間および95℃で15秒間、60℃で30秒間の45サイクル（光学機器をオンにする）。
JP2002566667A 2001-02-16 2001-10-05 核酸の磁気単離および精製の方法 Expired - Lifetime JP4399164B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26972901P 2001-02-16 2001-02-16
PCT/US2001/031637 WO2002066993A1 (en) 2001-02-16 2001-10-05 Magnetic isolation and purification of nucleic acids

Publications (3)

Publication Number Publication Date
JP2004523238A JP2004523238A (ja) 2004-08-05
JP2004523238A5 true JP2004523238A5 (ja) 2006-01-05
JP4399164B2 JP4399164B2 (ja) 2010-01-13

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ID=23028437

Family Applications (1)

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JP2002566667A Expired - Lifetime JP4399164B2 (ja) 2001-02-16 2001-10-05 核酸の磁気単離および精製の方法

Country Status (8)

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EP (3) EP1368629B1 (ja)
JP (1) JP4399164B2 (ja)
AT (1) ATE422542T1 (ja)
AU (1) AU2001296762B2 (ja)
CA (1) CA2438066A1 (ja)
DE (1) DE60137652D1 (ja)
ES (1) ES2322841T3 (ja)
WO (1) WO2002066993A1 (ja)

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Publication number Priority date Publication date Assignee Title
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US6855499B1 (en) 2001-02-16 2005-02-15 Cortex Biochem, Inc. Magnetic isolation and purification of nucleic acids
CA2465961A1 (en) * 2001-11-06 2003-05-15 Cortex Biochem, Inc. Isolation and purification of nucleic acids
JP3983125B2 (ja) 2002-07-19 2007-09-26 富士フイルム株式会社 核酸の分離精製方法
EP1510577A1 (en) 2003-08-29 2005-03-02 Qiagen GmbH Method for magnetic bead isolation of nucleic acids
JP4284250B2 (ja) * 2003-09-09 2009-06-24 富士フイルム株式会社 核酸分離精製方法
US7572578B2 (en) 2003-09-09 2009-08-11 Fujifilm Corporation Method for isolating and purifying a nucleic acid
US20050106602A1 (en) 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Simplified methods for isolating nucleic acids from cellular materials
US8426126B2 (en) 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
DE102007009347B4 (de) 2007-02-27 2009-11-26 Agowa Gmbh Verfahren zur Isolierung von Nukleinsäuren
EP2157181A1 (de) 2008-08-13 2010-02-24 AGOWA Gesellschaft für molekularbiologische Technologie mbH Verfahren zur Isolation von Nukleinsäuren und Testkit
US8288520B2 (en) 2008-10-27 2012-10-16 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and system
CA2760542A1 (en) 2009-05-01 2010-11-04 Qiagen Gaithersburg, Inc. A non-target amplification method for detection of rna splice-forms in a sample
EP2478087B1 (en) * 2009-09-14 2017-01-18 QIAGEN Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
BR112012018545A2 (pt) 2010-01-29 2016-05-03 Qiagen Gaithersburg Inc método de determinação e confirmação da presença de um hpv em uma amostra
WO2011094514A1 (en) 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
JP2013528049A (ja) 2010-05-19 2013-07-08 キアゲン ガイサーズバーグ アイエヌシー． 核酸の配列特異的な精製及び多重分析のための方法及び組成物
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
CA2828224A1 (en) 2011-02-24 2012-08-30 Qiagen Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acids
DE102012012523B4 (de) 2012-06-26 2015-02-12 Magnamedics Gmbh Reinigung von Nukleinsäuren
US11525155B2 (en) 2013-03-18 2022-12-13 Qiagen Gmbh Stabilisation of biological samples
JP6736463B2 (ja) 2013-12-02 2020-08-05 バイオカーティス エヌ ヴイ 循環核酸の抽出
JP2017537616A (ja) * 2014-11-14 2017-12-21 コーニング インコーポレイテッド インビトロ転写後のｒｎａ精製のための方法及びキット
US20180135040A1 (en) 2016-02-16 2018-05-17 Life Magnetics, Inc. Methods for separating nucleic acids with graphene coated magnetic beads

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8507706D0 (en) * 1985-03-25 1985-05-01 Genetics Int Inc Magnetic nucleic acid sequences
US4921805A (en) * 1987-07-29 1990-05-01 Life Technologies, Inc. Nucleic acid capture method
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
EP0411944B1 (en) * 1989-08-04 1998-06-10 BEHRINGWERKE Aktiengesellschaft Heterogeneous binding assays
GB9003253D0 (en) 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
US5705628A (en) * 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles

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