JP2004520812A - 対立遺伝子を決定するための方法 - Google Patents
対立遺伝子を決定するための方法 Download PDFInfo
- Publication number
- JP2004520812A JP2004520812A JP2002522564A JP2002522564A JP2004520812A JP 2004520812 A JP2004520812 A JP 2004520812A JP 2002522564 A JP2002522564 A JP 2002522564A JP 2002522564 A JP2002522564 A JP 2002522564A JP 2004520812 A JP2004520812 A JP 2004520812A
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- primer
- sequence
- site
- hetero
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 108700028369 Alleles Proteins 0.000 title claims abstract description 106
- 238000000034 method Methods 0.000 title claims abstract description 88
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 127
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 115
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 115
- 102000054766 genetic haplotypes Human genes 0.000 claims abstract description 12
- 239000011324 bead Substances 0.000 claims description 54
- 238000009396 hybridization Methods 0.000 claims description 38
- 125000005842 heteroatom Chemical group 0.000 claims description 30
- 230000000295 complement effect Effects 0.000 claims description 21
- 238000001514 detection method Methods 0.000 claims description 21
- 239000002773 nucleotide Substances 0.000 claims description 13
- 125000003729 nucleotide group Chemical group 0.000 claims description 13
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 11
- 238000010438 heat treatment Methods 0.000 claims 1
- 238000011895 specific detection Methods 0.000 claims 1
- 238000000926 separation method Methods 0.000 abstract description 3
- 230000000903 blocking effect Effects 0.000 abstract 1
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 50
- 108020004414 DNA Proteins 0.000 description 44
- 239000011616 biotin Substances 0.000 description 25
- 229960002685 biotin Drugs 0.000 description 25
- 235000020958 biotin Nutrition 0.000 description 25
- 239000012634 fragment Substances 0.000 description 23
- 239000000523 sample Substances 0.000 description 23
- 238000006243 chemical reaction Methods 0.000 description 21
- 102000053602 DNA Human genes 0.000 description 18
- 108090000623 proteins and genes Proteins 0.000 description 17
- 108090001008 Avidin Proteins 0.000 description 12
- 238000010586 diagram Methods 0.000 description 12
- 238000003752 polymerase chain reaction Methods 0.000 description 11
- 238000007844 allele-specific PCR Methods 0.000 description 9
- 238000013459 approach Methods 0.000 description 9
- 239000000203 mixture Substances 0.000 description 8
- 230000003321 amplification Effects 0.000 description 7
- 238000003556 assay Methods 0.000 description 7
- 238000003199 nucleic acid amplification method Methods 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 108091034117 Oligonucleotide Proteins 0.000 description 6
- 108020004682 Single-Stranded DNA Proteins 0.000 description 6
- 102000054765 polymorphisms of proteins Human genes 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108010090804 Streptavidin Proteins 0.000 description 5
- 230000001351 cycling effect Effects 0.000 description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 230000000692 anti-sense effect Effects 0.000 description 4
- 108010058966 bacteriophage T7 induced DNA polymerase Proteins 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 238000007846 asymmetric PCR Methods 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 239000011541 reaction mixture Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 2
- 102100028972 HLA class I histocompatibility antigen, A alpha chain Human genes 0.000 description 2
- 108010075704 HLA-A Antigens Proteins 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 108700005089 MHC Class I Genes Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 125000000217 alkyl group Chemical group 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 2
- 239000012148 binding buffer Substances 0.000 description 2
- 238000005251 capillar electrophoresis Methods 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 2
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 108010055863 gene b exonuclease Proteins 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000004005 microsphere Substances 0.000 description 2
- 239000011535 reaction buffer Substances 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 125000006850 spacer group Chemical group 0.000 description 2
- 230000005945 translocation Effects 0.000 description 2
- 241000938605 Crocodylia Species 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 102100028976 HLA class I histocompatibility antigen, B alpha chain Human genes 0.000 description 1
- 108010058607 HLA-B Antigens Proteins 0.000 description 1
- 108010052199 HLA-C Antigens Proteins 0.000 description 1
- 108700005092 MHC Class II Genes Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 108020005196 Mitochondrial DNA Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 108700042075 T-Cell Receptor Genes Proteins 0.000 description 1
- 241000589596 Thermus Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 239000000370 acceptor Substances 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 150000001350 alkyl halides Chemical class 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 210000003578 bacterial chromosome Anatomy 0.000 description 1
- 150000001615 biotins Chemical class 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000005546 dideoxynucleotide Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 210000001840 diploid cell Anatomy 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 238000002515 oligonucleotide synthesis Methods 0.000 description 1
- 230000008775 paternal effect Effects 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 125000005642 phosphothioate group Chemical group 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US22899400P | 2000-08-30 | 2000-08-30 | |
| PCT/US2001/041956 WO2002018659A2 (en) | 2000-08-30 | 2001-08-30 | Method for determining alleles |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2004520812A true JP2004520812A (ja) | 2004-07-15 |
| JP2004520812A5 JP2004520812A5 (enExample) | 2005-03-17 |
Family
ID=22859388
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002522564A Withdrawn JP2004520812A (ja) | 2000-08-30 | 2001-08-30 | 対立遺伝子を決定するための方法 |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JP2004520812A (enExample) |
| CN (1) | CN1293204C (enExample) |
| AU (1) | AU2001289177A1 (enExample) |
| CA (1) | CA2421078A1 (enExample) |
| WO (1) | WO2002018659A2 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011072222A (ja) * | 2009-09-29 | 2011-04-14 | Kitasato Otsuka Biomedical Assay Kenkyusho:Kk | 標的核酸の検出方法 |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030124592A1 (en) * | 2001-10-24 | 2003-07-03 | Bioprofile, Llc | Methods for detecting genetic haplotypes by interaction with probes |
| CA2915124C (en) | 2001-11-13 | 2018-08-14 | The Trustees Of The University Of Pennsylvania | A method of detecting and/or identifying adeno-associated virus (aav) sequences and isolating novel sequences identified thereby |
| ES2602352T3 (es) | 2001-12-17 | 2017-02-20 | The Trustees Of The University Of Pennsylvania | Secuencias de serotipo 8 de virus adenoasociado (VAA), vectores que las contienen y usos de las mismas |
| JP4769417B2 (ja) | 2001-12-17 | 2011-09-07 | ザ・トラステイーズ・オブ・ザ・ユニバーシテイ・オブ・ペンシルベニア | アデノ随伴ウイルス（ａａｖ）血清型９の配列、それを含むベクターおよびその使用 |
| JP3752466B2 (ja) | 2002-04-24 | 2006-03-08 | 株式会社日立製作所 | 遺伝子検査方法 |
| EP1536021A1 (en) * | 2003-11-27 | 2005-06-01 | Consortium National de Recherche en Genomique (CNRG) | Method for HLA typing |
| GB0406863D0 (en) * | 2004-03-26 | 2004-04-28 | Qiagen As | Nucleic acid sequencing |
| WO2005118862A2 (en) * | 2004-04-30 | 2005-12-15 | Applera Corporation | Compositions, methods, and kits for (mis)ligating oligonucleotides |
| GB0600116D0 (en) * | 2006-01-05 | 2006-02-15 | Univ Cardiff | Allele-specific sequencing |
| MX2010001440A (es) * | 2007-08-07 | 2010-05-20 | Monsanto Technology Llc | Metodos y composiciones para seleccionar plantas de soja resistentes a nematodos de nudos radiculares del sur. |
| EP3249053A1 (en) * | 2009-03-27 | 2017-11-29 | Life Technologies Corporation | Methods, compositions, and kits for detecting allelic variants |
| JP5795341B2 (ja) | 2010-03-08 | 2015-10-14 | デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド | 参照ブロック配列によるｆｕｌｌＣＯＬＤ−ＰＣＲ濃縮 |
| AU2012223438A1 (en) * | 2011-02-28 | 2013-09-26 | Transgenomic, Inc | Kit and method for sequencing a target DNA in a mixed population |
| CA2830361C (en) | 2011-03-31 | 2021-03-16 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable multiplex cold-pcr |
| US9133490B2 (en) | 2012-05-16 | 2015-09-15 | Transgenomic, Inc. | Step-up method for COLD-PCR enrichment |
| US10913977B2 (en) | 2013-07-24 | 2021-02-09 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR |
| RS60902B1 (sr) | 2014-03-09 | 2020-11-30 | Univ Pennsylvania | Kompozicije korisne u lečenju nedostatka ornitinske transkarbamilaze (otc) |
| US11371090B2 (en) | 2016-12-12 | 2022-06-28 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection |
| US11174511B2 (en) | 2017-07-24 | 2021-11-16 | Dana-Farber Cancer Institute, Inc. | Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture |
| WO2025049432A1 (en) * | 2023-08-28 | 2025-03-06 | Intellia Therapeutics, Inc. | Methods for editing hla-a in cells pre-screened for the absence of one or both alleles of hla-h*01 |
| CN118207338B (zh) * | 2024-05-20 | 2024-09-13 | 杭州迪谱医学检验实验室有限公司 | 一种基于核酸质谱的多重超敏体细胞突变检测方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3021036B2 (ja) * | 1989-02-13 | 2000-03-15 | ジェネコ・ピーティーワイ・リミテッド | 核酸配列又はその中の変化の検出 |
| US5468611A (en) * | 1990-06-27 | 1995-11-21 | The Blood Center Research Foundation, Inc. | Method for HLA typing |
| CA2115342C (en) * | 1992-06-17 | 2003-08-26 | Robert B. Wallace | A method of detecting and discriminating between nucleic acid sequences |
| GB9503808D0 (en) * | 1995-02-24 | 1995-04-12 | Univ Nottingham | Detection assay |
| EP2368897B1 (en) * | 1996-02-09 | 2016-10-19 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| AU4599797A (en) * | 1996-09-24 | 1998-04-17 | Qiagen Genomics, Inc. | Compositions and methods for enhancing hybridization specificity |
-
2001
- 2001-08-30 WO PCT/US2001/041956 patent/WO2002018659A2/en not_active Ceased
- 2001-08-30 AU AU2001289177A patent/AU2001289177A1/en not_active Abandoned
- 2001-08-30 CA CA002421078A patent/CA2421078A1/en not_active Abandoned
- 2001-08-30 JP JP2002522564A patent/JP2004520812A/ja not_active Withdrawn
- 2001-08-30 CN CNB018183670A patent/CN1293204C/zh not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011072222A (ja) * | 2009-09-29 | 2011-04-14 | Kitasato Otsuka Biomedical Assay Kenkyusho:Kk | 標的核酸の検出方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2002018659A3 (en) | 2003-07-31 |
| CN1501982A (zh) | 2004-06-02 |
| AU2001289177A1 (en) | 2002-03-13 |
| CA2421078A1 (en) | 2002-03-07 |
| CN1293204C (zh) | 2007-01-03 |
| WO2002018659A2 (en) | 2002-03-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2004520812A (ja) | 対立遺伝子を決定するための方法 | |
| US20240368682A1 (en) | Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications | |
| US6830884B1 (en) | Method of amplification | |
| US7914989B2 (en) | Capture moieties for nucleic acids and uses thereof | |
| AU697642B2 (en) | High throughput screening method for sequences or genetic alterations in nucleic acids | |
| CN1863927B (zh) | 核酸检测分析 | |
| US20050064459A1 (en) | Ligation assay | |
| US20040106108A1 (en) | Solid support assay systems and methods utilizing non-standard bases | |
| US20010014450A1 (en) | Detection of differences in nucleic acids | |
| WO1996003529A9 (en) | High throughput screening method for sequences or genetic alterations in nucleic acids | |
| WO1992016657A1 (en) | Method of identifying a nucleotide present at a defined position in a nucleic acid | |
| JP2004521634A (ja) | ＭｕｔＳおよびＲｅｃＡを使用する変異検出 | |
| US20040166493A1 (en) | Methods for variation detection | |
| WO2005093101A1 (en) | Nucleic acid sequencing | |
| US20030082549A1 (en) | Method for determining alleles | |
| Best et al. | Molecular pathology methods | |
| JP2003522527A (ja) | 一塩基多型の検出 | |
| Smith-Zagone et al. | Molecular pathology methods | |
| JP4731081B2 (ja) | 核酸を選択的に単離するための方法 | |
| US20080305470A1 (en) | Nucleic Acid Sequencing | |
| WO2025078657A1 (en) | Amplification-free target enrichment workflow for direct detection of nucleic acid modifications | |
| US20110257018A1 (en) | Nucleic acid sequencing | |
| WO2003070977A2 (en) | Method for detecting single nucleotide polymorphisms | |
| WO2001032929A1 (en) | Methods and compositions for analysis of snps and strs | |
| EP1756300A1 (en) | Nucleic acid sequencing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A711 | Notification of change in applicant |
Free format text: JAPANESE INTERMEDIATE CODE: A711 Effective date: 20040903 |
|
| A300 | Application deemed to be withdrawn because no request for examination was validly filed |
Free format text: JAPANESE INTERMEDIATE CODE: A300 Effective date: 20081104 |