CA2421078A1 - Method for determining alleles - Google Patents

Method for determining alleles Download PDF

Info

Publication number
CA2421078A1
CA2421078A1 CA002421078A CA2421078A CA2421078A1 CA 2421078 A1 CA2421078 A1 CA 2421078A1 CA 002421078 A CA002421078 A CA 002421078A CA 2421078 A CA2421078 A CA 2421078A CA 2421078 A1 CA2421078 A1 CA 2421078A1
Authority
CA
Canada
Prior art keywords
primer
nucleic acid
heterosequence
site
hybridized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002421078A
Other languages
English (en)
French (fr)
Inventor
Xiangjun Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies AS
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2421078A1 publication Critical patent/CA2421078A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002421078A 2000-08-30 2001-08-30 Method for determining alleles Abandoned CA2421078A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22899400P 2000-08-30 2000-08-30
US60/228,994 2000-08-30
PCT/US2001/041956 WO2002018659A2 (en) 2000-08-30 2001-08-30 Method for determining alleles

Publications (1)

Publication Number Publication Date
CA2421078A1 true CA2421078A1 (en) 2002-03-07

Family

ID=22859388

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002421078A Abandoned CA2421078A1 (en) 2000-08-30 2001-08-30 Method for determining alleles

Country Status (5)

Country Link
JP (1) JP2004520812A (enExample)
CN (1) CN1293204C (enExample)
AU (1) AU2001289177A1 (enExample)
CA (1) CA2421078A1 (enExample)
WO (1) WO2002018659A2 (enExample)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030124592A1 (en) * 2001-10-24 2003-07-03 Bioprofile, Llc Methods for detecting genetic haplotypes by interaction with probes
CA2915124C (en) 2001-11-13 2018-08-14 The Trustees Of The University Of Pennsylvania A method of detecting and/or identifying adeno-associated virus (aav) sequences and isolating novel sequences identified thereby
ES2602352T3 (es) 2001-12-17 2017-02-20 The Trustees Of The University Of Pennsylvania Secuencias de serotipo 8 de virus adenoasociado (VAA), vectores que las contienen y usos de las mismas
JP4769417B2 (ja) 2001-12-17 2011-09-07 ザ・トラステイーズ・オブ・ザ・ユニバーシテイ・オブ・ペンシルベニア アデノ随伴ウイルス（ａａｖ）血清型９の配列、それを含むベクターおよびその使用
JP3752466B2 (ja) 2002-04-24 2006-03-08 株式会社日立製作所 遺伝子検査方法
EP1536021A1 (en) * 2003-11-27 2005-06-01 Consortium National de Recherche en Genomique (CNRG) Method for HLA typing
GB0406863D0 (en) * 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
WO2005118862A2 (en) * 2004-04-30 2005-12-15 Applera Corporation Compositions, methods, and kits for (mis)ligating oligonucleotides
GB0600116D0 (en) * 2006-01-05 2006-02-15 Univ Cardiff Allele-specific sequencing
MX2010001440A (es) * 2007-08-07 2010-05-20 Monsanto Technology Llc Metodos y composiciones para seleccionar plantas de soja resistentes a nematodos de nudos radiculares del sur.
EP3249053A1 (en) * 2009-03-27 2017-11-29 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
JP2011072222A (ja) * 2009-09-29 2011-04-14 Kitasato Otsuka Biomedical Assay Kenkyusho:Kk 標的核酸の検出方法
JP5795341B2 (ja) 2010-03-08 2015-10-14 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド 参照ブロック配列によるｆｕｌｌＣＯＬＤ−ＰＣＲ濃縮
AU2012223438A1 (en) * 2011-02-28 2013-09-26 Transgenomic, Inc Kit and method for sequencing a target DNA in a mixed population
CA2830361C (en) 2011-03-31 2021-03-16 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex cold-pcr
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
RS60902B1 (sr) 2014-03-09 2020-11-30 Univ Pennsylvania Kompozicije korisne u lečenju nedostatka ornitinske transkarbamilaze (otc)
US11371090B2 (en) 2016-12-12 2022-06-28 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
WO2025049432A1 (en) * 2023-08-28 2025-03-06 Intellia Therapeutics, Inc. Methods for editing hla-a in cells pre-screened for the absence of one or both alleles of hla-h*01
CN118207338B (zh) * 2024-05-20 2024-09-13 杭州迪谱医学检验实验室有限公司 一种基于核酸质谱的多重超敏体细胞突变检测方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3021036B2 (ja) * 1989-02-13 2000-03-15 ジェネコ・ピーティーワイ・リミテッド 核酸配列又はその中の変化の検出
US5468611A (en) * 1990-06-27 1995-11-21 The Blood Center Research Foundation, Inc. Method for HLA typing
CA2115342C (en) * 1992-06-17 2003-08-26 Robert B. Wallace A method of detecting and discriminating between nucleic acid sequences
GB9503808D0 (en) * 1995-02-24 1995-04-12 Univ Nottingham Detection assay
EP2368897B1 (en) * 1996-02-09 2016-10-19 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
AU4599797A (en) * 1996-09-24 1998-04-17 Qiagen Genomics, Inc. Compositions and methods for enhancing hybridization specificity

Also Published As

Publication number Publication date
WO2002018659A3 (en) 2003-07-31
CN1501982A (zh) 2004-06-02
AU2001289177A1 (en) 2002-03-13
JP2004520812A (ja) 2004-07-15
CN1293204C (zh) 2007-01-03
WO2002018659A2 (en) 2002-03-07

Similar Documents

Publication Publication Date Title
CA2421078A1 (en) Method for determining alleles
US9133516B2 (en) Methods for identification of alleles using allele-specific primers for amplification
JP4499987B2 (ja) 非−標準塩基を使用する固体支持体アッセイ系及び方法
US20060088826A1 (en) Discrimination and detection of target nucleotide sequences using mass spectrometry
US20140364323A1 (en) Multi-sample indexing for multiplex genotyping
US7638310B2 (en) Method to determine single nucleotide polymorphisms and mutations in nucleic acid sequence
JP2000511434A (ja) 人工的ミスマッチハイブリダイゼーション
US7189512B2 (en) Methods for variation detection
CA2318371A1 (en) Method for the detection of nucleic acid sequences
US20060073511A1 (en) Methods for amplifying and analyzing nucleic acids
WO2005093101A1 (en) Nucleic acid sequencing
US20030082549A1 (en) Method for determining alleles
WO1998038338A1 (en) Nucleic acid analysis methods
JP2003511056A (ja) ５−位置メチル化変性体の識別方法
JP2003511056A5 (enExample)
CN1434873B (zh) 选择性分离核酸的方法
US20080305470A1 (en) Nucleic Acid Sequencing
WO2003070977A2 (en) Method for detecting single nucleotide polymorphisms
US20110257018A1 (en) Nucleic acid sequencing
US20040241697A1 (en) Compositions and methods to identify haplotypes
Park et al. DNA Microarray‐Based Technologies to Genotype Single Nucleotide Polymorphisms
WO2001032929A1 (en) Methods and compositions for analysis of snps and strs
HK1066246A (en) Method for determining alleles
WO2002024960A1 (en) Detection of dna variation

Legal Events

Date Code Title Description
EEER Examination request
FZDE Dead