CA2421078A1 - Procede servant a determiner des alleles - Google Patents
Procede servant a determiner des alleles Download PDFInfo
- Publication number
- CA2421078A1 CA2421078A1 CA002421078A CA2421078A CA2421078A1 CA 2421078 A1 CA2421078 A1 CA 2421078A1 CA 002421078 A CA002421078 A CA 002421078A CA 2421078 A CA2421078 A CA 2421078A CA 2421078 A1 CA2421078 A1 CA 2421078A1
- Authority
- CA
- Canada
- Prior art keywords
- primer
- nucleic acid
- heterosequence
- site
- hybridized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne des procédés et des trousses servant à séparer et à identifier des allèles et, par conséquent, l'haplotype, dans des spécimens d'ADN génomique. Ce procédé consiste généralement à effectuer l'hybridation d'amorces spécifiques pour des sites polymorphes à l'intérieur de ces allèles aux spécimens d'ADN, à prolonger ces amorces par un ou plusieurs acides nucléiques, à séparer les amorces prolongées et à identifier les allèles au moyen de l'amorce prolongée. Ce procédé permet également d'effectuer la ligation de deux amorces, leur séparation et leur utilisation ultérieure afin d'identifier l'allèle ciblé. Ce procédé permet, de plus, d'utiliser une autre amorce en tant que site bloquant pour l'extension de la première amorce, de façon à répliquer et à identifier un segment d'ADN comprenant un site polymorphe. On peut marquer les amorces prolongées ou non, de manière à pouvoir séparer et/ou identifier l'amorce sans difficultés.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US22899400P | 2000-08-30 | 2000-08-30 | |
US60/228,994 | 2000-08-30 | ||
PCT/US2001/041956 WO2002018659A2 (fr) | 2000-08-30 | 2001-08-30 | Procede servant a determiner des alleles |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2421078A1 true CA2421078A1 (fr) | 2002-03-07 |
Family
ID=22859388
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002421078A Abandoned CA2421078A1 (fr) | 2000-08-30 | 2001-08-30 | Procede servant a determiner des alleles |
Country Status (5)
Country | Link |
---|---|
JP (1) | JP2004520812A (fr) |
CN (1) | CN1293204C (fr) |
AU (1) | AU2001289177A1 (fr) |
CA (1) | CA2421078A1 (fr) |
WO (1) | WO2002018659A2 (fr) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2002343573A1 (en) * | 2001-10-24 | 2003-05-06 | Singulex, Inc. | Methods for detecting genetic haplotypes by interaction with probes |
HU230406B1 (hu) | 2001-11-13 | 2016-04-28 | The Trustees Of The University Of Pennsylvania | Eljárás adeno-asszociált vírus(AAV)szekvenciák detektálására és/vagy azonosítására és az azzal azonosított új szekvenciák izolálására |
EP3339430A1 (fr) | 2001-12-17 | 2018-06-27 | The Trustees of The University of Pennsylvania | Séquences de sérotype 9 de virus adéno associé, vecteurs les contenant et leurs utilisations |
AU2002360291A1 (en) | 2001-12-17 | 2003-06-30 | The Trustees Of The University Of Pennsylvania | Adeno-associated virus (aav) serotype 8 sequences |
JP3752466B2 (ja) | 2002-04-24 | 2006-03-08 | 株式会社日立製作所 | 遺伝子検査方法 |
EP1536021A1 (fr) * | 2003-11-27 | 2005-06-01 | Consortium National de Recherche en Genomique (CNRG) | Méthode pour le typage de HLA |
GB0406863D0 (en) * | 2004-03-26 | 2004-04-28 | Qiagen As | Nucleic acid sequencing |
US20050282195A1 (en) * | 2004-04-30 | 2005-12-22 | Applera Corporation | Compositions, methods, and kits for (MIS)ligating oligonucleotides |
GB0600116D0 (en) * | 2006-01-05 | 2006-02-15 | Univ Cardiff | Allele-specific sequencing |
CN107090468B (zh) * | 2007-08-07 | 2021-07-09 | 孟山都技术公司 | 用于选择南方根结线虫抗性大豆植物的方法和组合物 |
CN103911428B (zh) * | 2009-03-27 | 2016-02-24 | 生命技术公司 | 用于检测等位基因变体的方法、组合物和试剂盒 |
JP2011072222A (ja) * | 2009-09-29 | 2011-04-14 | Kitasato Otsuka Biomedical Assay Kenkyusho:Kk | 標的核酸の検出方法 |
KR101550489B1 (ko) | 2010-03-08 | 2015-09-07 | 다나-파버 캔서 인스티튜트 인크. | 기준 차단 서열을 이용한 완전 cold-pcr 풍부화 |
AU2012223438A1 (en) * | 2011-02-28 | 2013-09-26 | Transgenomic, Inc | Kit and method for sequencing a target DNA in a mixed population |
EP3333269B1 (fr) | 2011-03-31 | 2021-05-05 | Dana-Farber Cancer Institute, Inc. | Procédés permettant d'activer un multiplexage froid-pcr |
US9133490B2 (en) | 2012-05-16 | 2015-09-15 | Transgenomic, Inc. | Step-up method for COLD-PCR enrichment |
WO2015013166A1 (fr) | 2013-07-24 | 2015-01-29 | Dana-Farber Cancer Institute, Inc. | Procédés et compositions pour permettre l'enrichissement d'allèles d'adn mineurs par limitation du temps de dénaturation en pcr ou permettre simplement l'enrichissement d'allèles d'adn mineurs par limitation du temps de dénaturation en pcr |
MX2016011686A (es) | 2014-03-09 | 2016-11-07 | Univ Pennsylvania | Composiciones utiles en el tratamiento de deficiencia de la ornitina transcarbamilasa (otc). |
EP3551756A4 (fr) | 2016-12-12 | 2020-07-15 | Dana Farber Cancer Institute, Inc. | Compositions et procédés pour le codage par code-barres moléculaire de molécules d'adn avant l'enrichissement des mutations et/ou la détection des mutations |
WO2019023243A1 (fr) | 2017-07-24 | 2019-01-31 | Dana-Farber Cancer Institute, Inc. | Procédés et compositions pour sélectionner et amplifier des cibles d'adn dans un mélange de réaction unique |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2136059T5 (es) * | 1989-02-13 | 2005-02-16 | Geneco Pty. Ltd. | Deteccion de una secuencia de acidos nucleicos o de un cambio en la misma. |
US5468611A (en) * | 1990-06-27 | 1995-11-21 | The Blood Center Research Foundation, Inc. | Method for HLA typing |
JPH06509946A (ja) * | 1992-06-17 | 1994-11-10 | シティ・オブ・ホープ | 核酸を検出し識別する方法 |
GB9503808D0 (en) * | 1995-02-24 | 1995-04-12 | Univ Nottingham | Detection assay |
EP2332958B1 (fr) * | 1996-02-09 | 2016-04-20 | Cornell Research Foundation, Inc. | Détection de différences entre séquences d'acide nucléique faisant appel à la réaction de détection par ligase et à des réseaux adressables |
EP0958378A2 (fr) * | 1996-09-24 | 1999-11-24 | Rapigene, Inc. | Compositions et procedes pour l'augmentation de la specificite d'hybridation |
-
2001
- 2001-08-30 WO PCT/US2001/041956 patent/WO2002018659A2/fr active Application Filing
- 2001-08-30 CN CNB018183670A patent/CN1293204C/zh not_active Expired - Fee Related
- 2001-08-30 JP JP2002522564A patent/JP2004520812A/ja not_active Withdrawn
- 2001-08-30 AU AU2001289177A patent/AU2001289177A1/en not_active Abandoned
- 2001-08-30 CA CA002421078A patent/CA2421078A1/fr not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
CN1293204C (zh) | 2007-01-03 |
JP2004520812A (ja) | 2004-07-15 |
WO2002018659A3 (fr) | 2003-07-31 |
AU2001289177A1 (en) | 2002-03-13 |
CN1501982A (zh) | 2004-06-02 |
WO2002018659A2 (fr) | 2002-03-07 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Dead |