JP2004254544A - 新規リジンデカルボキシラーゼ遺伝子及びｌ−リジンの製造法 - Google Patents
新規リジンデカルボキシラーゼ遺伝子及びｌ−リジンの製造法 Download PDFInfo
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- JP2004254544A JP2004254544A JP2003047185A JP2003047185A JP2004254544A JP 2004254544 A JP2004254544 A JP 2004254544A JP 2003047185 A JP2003047185 A JP 2003047185A JP 2003047185 A JP2003047185 A JP 2003047185A JP 2004254544 A JP2004254544 A JP 2004254544A
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- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 229910000148 ammonium phosphate Inorganic materials 0.000 description 1
- 235000019289 ammonium phosphates Nutrition 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
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- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000000911 decarboxylating effect Effects 0.000 description 1
- 238000006114 decarboxylation reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 238000012224 gene deletion Methods 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- -1 iron ions Chemical class 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229910001437 manganese ion Inorganic materials 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000037434 nonsense mutation Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 125000001477 organic nitrogen group Chemical group 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000013587 production medium Substances 0.000 description 1
- 229940107700 pyruvic acid Drugs 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 101150079601 recA gene Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
- 235000019345 sodium thiosulphate Nutrition 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000001384 succinic acid Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2003047185A JP2004254544A (ja) | 2003-02-25 | 2003-02-25 | 新規リジンデカルボキシラーゼ遺伝子及びｌ−リジンの製造法 |
| DE102004006526A DE102004006526A1 (de) | 2003-02-25 | 2004-02-10 | Neues Lysindecarboxylase-Gen und Verfahren zur Herstellung von L-Lysin |
| US10/784,986 US20040229311A1 (en) | 2003-02-25 | 2004-02-25 | Novel lysine decarboxylase gene and method for producing L-lysine |
| FR0401873A FR2851575A1 (fr) | 2003-02-25 | 2004-02-25 | Nouveau gene de la lysine decarboxylase et procede de production de la l-lysine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2003047185A JP2004254544A (ja) | 2003-02-25 | 2003-02-25 | 新規リジンデカルボキシラーゼ遺伝子及びｌ−リジンの製造法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2004254544A true JP2004254544A (ja) | 2004-09-16 |
| JP2004254544A5 JP2004254544A5 (enExample) | 2006-03-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003047185A Pending JP2004254544A (ja) | 2003-02-25 | 2003-02-25 | 新規リジンデカルボキシラーゼ遺伝子及びｌ−リジンの製造法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20040229311A1 (enExample) |
| JP (1) | JP2004254544A (enExample) |
| DE (1) | DE102004006526A1 (enExample) |
| FR (1) | FR2851575A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007114465A1 (ja) | 2006-03-30 | 2007-10-11 | Ajinomoto Co., Inc. | メタノール資化性細菌を用いたカルボン酸の製造法 |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001046067A (ja) * | 1999-08-04 | 2001-02-20 | Ajinomoto Co Inc | 好熱性バチルス属細菌由来のｌ−リジン生合成系遺伝子 |
| DE60232120D1 (de) * | 2001-06-12 | 2009-06-10 | Ajinomoto Kk | Verfahren zur Herstellung von L-Lysin oder L-Arginin unter Verwendung methanolassimilierender Bakterien |
| US20050176121A1 (en) * | 2001-09-06 | 2005-08-11 | Ryo Takeshita | Method for producing alcohol by using microorganism |
| JP2004166595A (ja) * | 2002-11-20 | 2004-06-17 | Ajinomoto Co Inc | メチロトローフを用いたｌ−アミノ酸の製造法 |
| JP4120364B2 (ja) * | 2002-11-20 | 2008-07-16 | 味の素株式会社 | メタノール資化性菌を用いたｌ−リジンまたはｌ−アルギニンの製造法 |
| US20040171134A1 (en) * | 2003-01-08 | 2004-09-02 | Takayuki Asahara | Method for producing recombinant of methanol-assimilating bacterium |
| DE102004001674B4 (de) * | 2003-01-29 | 2019-01-24 | Ajinomoto Co., Inc. | Verfahren zur Herstellung von L-Lysin unter Einsatz von Methanol verwertenden Bakterien |
| JP2004236637A (ja) * | 2003-02-10 | 2004-08-26 | Ajinomoto Co Inc | 多糖類生成に関与する遺伝子及びその利用 |
| JP2004261150A (ja) * | 2003-03-04 | 2004-09-24 | Ajinomoto Co Inc | 目的物質の製造法 |
| RU2004124226A (ru) | 2004-08-10 | 2006-01-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) | Использование фосфокетолазы для продукции полезных метаболитов |
| US7205132B2 (en) * | 2004-09-10 | 2007-04-17 | Ajinomoto Co., Inc. | L-glutamic acid-producing microorganism and a method for producing L-glutamic acid |
| US7794989B2 (en) * | 2004-12-28 | 2010-09-14 | Ajinomoto Co., Inc. | L-glutamic acid-producing microorganism and a method for producing L-glutamic acid |
| US20070244719A1 (en) * | 2006-04-13 | 2007-10-18 | David Peter R | Compositions and methods for producing fermentation products and residuals |
| GB2439310A (en) * | 2006-04-13 | 2007-12-27 | Ambrozea Inc | Fermentation residuals from genetically-modified microorganisms |
| US7309602B2 (en) * | 2006-04-13 | 2007-12-18 | Ambrozea, Inc. | Compositions and methods for producing fermentation products and residuals |
| US20070243235A1 (en) * | 2006-04-13 | 2007-10-18 | David Peter R | Compositions and methods for producing fermentation products and residuals |
| WO2007139219A1 (en) * | 2006-06-01 | 2007-12-06 | Ajinomoto Co., Inc. | A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family with attenuated expression of the rcsa gene |
| US8839078B2 (en) * | 2010-03-05 | 2014-09-16 | Samsung Electronics Co., Ltd. | Application layer FEC framework for WiGig |
| CN115595314B (zh) * | 2022-03-07 | 2025-06-24 | 中国科学院微生物研究所 | 表达天冬氨酸脱氢酶的工程菌及发酵生产维生素b5的方法 |
| CN114875088A (zh) * | 2022-04-11 | 2022-08-09 | 绵阳晟氏健康科技有限公司 | 一种d-赖氨酸的制备方法 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3046332B2 (ja) * | 1990-08-02 | 2000-05-29 | 協和醗酵工業株式会社 | 発酵法によるアミノ酸の製造法 |
| EP0796912B9 (en) * | 1994-12-09 | 2013-08-28 | Ajinomoto Co., Inc. | Novel lysine decarboxylase gene and process for producing l-lysine |
| JP2001046067A (ja) * | 1999-08-04 | 2001-02-20 | Ajinomoto Co Inc | 好熱性バチルス属細菌由来のｌ−リジン生合成系遺伝子 |
| JP2001120292A (ja) * | 1999-08-17 | 2001-05-08 | Ajinomoto Co Inc | 発酵法によるｌ−アミノ酸の製造法 |
| JP2001120269A (ja) * | 1999-10-22 | 2001-05-08 | Ajinomoto Co Inc | 発酵法によるｌ−リジンの製造法 |
| DE60232120D1 (de) * | 2001-06-12 | 2009-06-10 | Ajinomoto Kk | Verfahren zur Herstellung von L-Lysin oder L-Arginin unter Verwendung methanolassimilierender Bakterien |
| US6911332B2 (en) * | 2002-06-12 | 2005-06-28 | Ajinomoto Co., Inc. | Isolated polynucleotides encoding d-arabino-3-hexulose-6-phosphate synthases from Methylophilus methylotrophus |
| JP2004166595A (ja) * | 2002-11-20 | 2004-06-17 | Ajinomoto Co Inc | メチロトローフを用いたｌ−アミノ酸の製造法 |
| JP2004166592A (ja) * | 2002-11-20 | 2004-06-17 | Ajinomoto Co Inc | メチロトローフを用いたｌ−アミノ酸の製造法 |
| US20040171134A1 (en) * | 2003-01-08 | 2004-09-02 | Takayuki Asahara | Method for producing recombinant of methanol-assimilating bacterium |
| DE102004001674B4 (de) * | 2003-01-29 | 2019-01-24 | Ajinomoto Co., Inc. | Verfahren zur Herstellung von L-Lysin unter Einsatz von Methanol verwertenden Bakterien |
| JP2004236637A (ja) * | 2003-02-10 | 2004-08-26 | Ajinomoto Co Inc | 多糖類生成に関与する遺伝子及びその利用 |
| US7029893B2 (en) * | 2003-02-28 | 2006-04-18 | Ajinomoto Co., Inc. | Polynucleotides encoding polypeptides involved in amino acid biosynthesis in methylophilus methylotrophus |
| US7026149B2 (en) * | 2003-02-28 | 2006-04-11 | Ajinomoto Co., Inc. | Polynucleotides encoding polypeptides involved in the stress response to environmental changes in Methylophilus methylotrophus |
| US7060475B2 (en) * | 2003-02-28 | 2006-06-13 | Ajinomoto Co., Inc. | Polynucleotides encoding polypeptides involved in intermediates metabolism of central metabolic pathway in methylophilus methylotrophus |
| JP2004261150A (ja) * | 2003-03-04 | 2004-09-24 | Ajinomoto Co Inc | 目的物質の製造法 |
-
2003
- 2003-02-25 JP JP2003047185A patent/JP2004254544A/ja active Pending
-
2004
- 2004-02-10 DE DE102004006526A patent/DE102004006526A1/de not_active Withdrawn
- 2004-02-25 US US10/784,986 patent/US20040229311A1/en not_active Abandoned
- 2004-02-25 FR FR0401873A patent/FR2851575A1/fr active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007114465A1 (ja) | 2006-03-30 | 2007-10-11 | Ajinomoto Co., Inc. | メタノール資化性細菌を用いたカルボン酸の製造法 |
Also Published As
| Publication number | Publication date |
|---|---|
| DE102004006526A1 (de) | 2004-09-02 |
| US20040229311A1 (en) | 2004-11-18 |
| FR2851575A1 (fr) | 2004-08-27 |
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