JP2003535564A5 - - Google Patents
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- Publication number
- JP2003535564A5 JP2003535564A5 JP2000512942A JP2000512942A JP2003535564A5 JP 2003535564 A5 JP2003535564 A5 JP 2003535564A5 JP 2000512942 A JP2000512942 A JP 2000512942A JP 2000512942 A JP2000512942 A JP 2000512942A JP 2003535564 A5 JP2003535564 A5 JP 2003535564A5
- Authority
- JP
- Japan
- Prior art keywords
- cell
- endogenous gene
- vector construct
- regulatory sequence
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cell Anatomy 0.000 description 139
- 108090000623 proteins and genes Proteins 0.000 description 108
- 238000000034 method Methods 0.000 description 53
- 230000001105 regulatory effect Effects 0.000 description 52
- 230000035897 transcription Effects 0.000 description 34
- 238000013518 transcription Methods 0.000 description 34
- 230000014509 gene expression Effects 0.000 description 23
- 230000006801 homologous recombination Effects 0.000 description 20
- 238000002744 homologous recombination Methods 0.000 description 20
- 230000002103 transcriptional effect Effects 0.000 description 17
- 102000004169 proteins and genes Human genes 0.000 description 14
- 108010076504 Protein Sorting Signals Proteins 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 9
- 238000012216 screening Methods 0.000 description 8
- 230000003248 secreting effect Effects 0.000 description 8
- 108091081024 Start codon Proteins 0.000 description 7
- 238000012258 culturing Methods 0.000 description 6
- 239000003550 marker Substances 0.000 description 6
- 230000006798 recombination Effects 0.000 description 6
- 239000002299 complementary DNA Substances 0.000 description 5
- 239000003623 enhancer Substances 0.000 description 5
- 238000005215 recombination Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 230000003827 upregulation Effects 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 4
- 239000003636 conditioned culture medium Substances 0.000 description 4
- 210000003527 eukaryotic cell Anatomy 0.000 description 4
- 230000014621 translational initiation Effects 0.000 description 4
- 238000011144 upstream manufacturing Methods 0.000 description 4
- 230000003612 virological effect Effects 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 230000003213 activating effect Effects 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000007876 drug discovery Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 230000002018 overexpression Effects 0.000 description 2
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 230000001177 retroviral effect Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 230000005026 transcription initiation Effects 0.000 description 2
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 108020005067 RNA Splice Sites Proteins 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US94122397A | 1997-09-26 | 1997-09-26 | |
| US08/941,223 | 1997-09-26 | ||
| US15964398A | 1998-09-24 | 1998-09-24 | |
| US09/159,643 | 1998-09-24 | ||
| PCT/US1998/020094 WO1999015650A1 (en) | 1997-09-26 | 1998-09-25 | Expression of endogenous genes by non-homologous recombination of a vector construct with cellular dna |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2003535564A JP2003535564A (ja) | 2003-12-02 |
| JP2003535564A5 true JP2003535564A5 (enExample) | 2006-01-26 |
| JP5377806B2 JP5377806B2 (ja) | 2013-12-25 |
Family
ID=26856150
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000512942A Expired - Lifetime JP5377806B2 (ja) | 1997-09-26 | 1998-09-25 | ベクター構築物の細胞ｄｎａとの非相同組換えによる内因性遺伝子の発現 |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP1017803B1 (enExample) |
| JP (1) | JP5377806B2 (enExample) |
| KR (1) | KR20010024296A (enExample) |
| CN (1) | CN100582230C (enExample) |
| AT (1) | ATE471373T1 (enExample) |
| AU (1) | AU750025B2 (enExample) |
| BR (1) | BR9812540A (enExample) |
| CA (1) | CA2304642C (enExample) |
| DE (1) | DE69841723D1 (enExample) |
| DK (1) | DK1017803T3 (enExample) |
| IL (2) | IL135242A0 (enExample) |
| NO (1) | NO20001497L (enExample) |
| NZ (1) | NZ504185A (enExample) |
| WO (1) | WO1999015650A1 (enExample) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6740503B1 (en) | 1997-09-26 | 2004-05-25 | Athersys, Inc. | Compositions and methods for non-targeted activation of endogenous genes |
| US6897066B1 (en) | 1997-09-26 | 2005-05-24 | Athersys, Inc. | Compositions and methods for non-targeted activation of endogenous genes |
| CA2364267A1 (en) * | 1999-02-19 | 2000-08-24 | Athersys, Inc. | Compositions and methods for non-targeted activation of endogenous genes |
| JP2003527833A (ja) | 1999-10-14 | 2003-09-24 | クロンテック・ラボラトリーズ・インコーポレーテッド | 花虫類に由来する発色団／蛍光体、およびそれらの使用法 |
| MXPA02012747A (es) | 2000-06-28 | 2003-09-25 | Amgen Inc | Moleculas del receptor de linfopoietina estromica timica y sus usos. |
| GB0018876D0 (en) * | 2000-08-01 | 2000-09-20 | Applied Research Systems | Method of producing polypeptides |
| EP1352060A2 (en) * | 2000-10-30 | 2003-10-15 | Novozymes Biotech, Inc. | Methods for expressing endogenous genes by restriction enzyme mediated integration |
| DK1451295T3 (en) | 2001-11-02 | 2015-07-06 | Athersys Inc | METHODS FOR MUTATION OF GENES IN CELLS AND ANIMALS USING THE INSERTION MUTAGENES. |
| EP1456223B1 (en) | 2001-12-19 | 2009-08-12 | The University Of Chicago | Rapidly maturing fluorescent proteins and methods for using the same |
| US7662924B2 (en) | 2002-02-22 | 2010-02-16 | The Board Of Trustees Of The University Of Illinois | Beta chain-associated regulator of apoptosis |
| CA2899360A1 (en) | 2002-08-21 | 2004-04-08 | Revivicor, Inc. | Porcine animals lacking any expression of functional alpha 1,3 galactosyltransferase |
| US9453251B2 (en) | 2002-10-08 | 2016-09-27 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
| US8021833B2 (en) | 2003-02-12 | 2011-09-20 | Functional Genetics, Inc. | Method for reducing HIV viral budding by administering a VPS28-specfic antibody that disrupts Gag-TSG101-VPS28 binding interactions |
| US7888121B2 (en) | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
| US11311574B2 (en) | 2003-08-08 | 2022-04-26 | Sangamo Therapeutics, Inc. | Methods and compositions for targeted cleavage and recombination |
| US8409861B2 (en) | 2003-08-08 | 2013-04-02 | Sangamo Biosciences, Inc. | Targeted deletion of cellular DNA sequences |
| CN104721883A (zh) | 2004-03-17 | 2015-06-24 | 雷维维科公司 | 来源于缺乏任何功能性α1,3半乳糖基转移酶表达的动物的组织产品 |
| US7250298B2 (en) | 2004-04-07 | 2007-07-31 | The University Of Chicago | Monomeric red fluorescent proteins |
| AU2005269527B2 (en) | 2004-07-26 | 2011-12-01 | Pfenex Inc. | Process for improved protein expression by strain engineering |
| ES2679282T3 (es) | 2004-10-22 | 2018-08-23 | Revivicor Inc. | Porcinos transgénicos que carecen de cadena ligera de inmunoglobulina endógena |
| PA8660701A1 (es) | 2005-02-04 | 2006-09-22 | Pfizer Prod Inc | Agonistas de pyy y sus usos |
| EP2399932B1 (en) | 2005-05-12 | 2016-11-16 | ZymoGenetics, Inc. | Compositions and methods for modulating immune responses |
| AU2006292827B2 (en) | 2005-08-09 | 2013-02-14 | Revivicor, Inc. | Transgenic ungulates expressing CTLA4-IG and uses thereof |
| WO2007053732A2 (en) | 2005-11-01 | 2007-05-10 | Mayo Foundation For Medical Education And Research | Promoter polymorphisms of the blys gene and use in diagnostic methods |
| US8014957B2 (en) | 2005-12-15 | 2011-09-06 | Fred Hutchinson Cancer Research Center | Genes associated with progression and response in chronic myeloid leukemia and uses thereof |
| JP5102833B2 (ja) | 2006-07-24 | 2012-12-19 | バイオレクシス ファーマシューティカル コーポレーション | エキセンディン融合タンパク質 |
| EP2615172A1 (en) | 2007-04-27 | 2013-07-17 | Pfenex Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
| US9580719B2 (en) | 2007-04-27 | 2017-02-28 | Pfenex, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
| WO2009059305A2 (en) | 2007-11-01 | 2009-05-07 | The University Of Chicago | Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation |
| WO2009073511A2 (en) | 2007-11-30 | 2009-06-11 | Mayo Foundation For Medical Education And Research | Polymorphisms of the blys gene and use in diagnostic methods |
| EP2525808A2 (en) | 2010-01-19 | 2012-11-28 | The Trustees Of Columbia University In The City Of New York | Osteocalcin as a treatment for male reproductive disorders |
| CN102669057B (zh) * | 2011-03-18 | 2014-11-26 | 上海南方模式生物科技发展有限公司 | 一种调控动物内源基因表达的遗传修饰方法 |
| KR101948248B1 (ko) * | 2011-11-18 | 2019-02-14 | 한국생명공학연구원 | 단백질 발현 미세 조절방법 |
| US10052364B2 (en) | 2013-03-15 | 2018-08-21 | The Trustees Of Columbia University In The City Of New York | Osteocalcin as a treatment for cognitive disorders |
| US9957515B2 (en) | 2013-03-15 | 2018-05-01 | Cibus Us Llc | Methods and compositions for targeted gene modification |
| US20170081674A1 (en) * | 2014-03-14 | 2017-03-23 | Cibus Us Llc | Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair |
| WO2016081728A1 (en) | 2014-11-19 | 2016-05-26 | The Trustees Of Columbia University In The City Of New York | Osteocalcin as a treatment for frailty associated with aging |
| EP3802788B1 (en) * | 2018-05-15 | 2025-07-02 | Duke University | Systems and methods for genetic manipulation of akkermansia species |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5024939A (en) * | 1987-07-09 | 1991-06-18 | Genentech, Inc. | Transient expression system for producing recombinant protein |
| WO1991006667A1 (en) * | 1989-11-06 | 1991-05-16 | Cell Genesys, Inc. | Production of proteins using homologous recombination |
| US5204939A (en) * | 1989-12-14 | 1993-04-20 | Fujitsu Limited | Rule base processing system and rule evaluation control method therein |
| US5272071A (en) * | 1989-12-22 | 1993-12-21 | Applied Research Systems Ars Holding N.V. | Method for the modification of the expression characteristics of an endogenous gene of a given cell line |
| US6270989B1 (en) * | 1991-11-05 | 2001-08-07 | Transkaryotic Therapies, Inc. | Protein production and delivery |
| US5641670A (en) * | 1991-11-05 | 1997-06-24 | Transkaryotic Therapies, Inc. | Protein production and protein delivery |
| US5561053A (en) * | 1994-08-05 | 1996-10-01 | Genentech, Inc. | Method for selecting high-expressing host cells |
| CA2364267A1 (en) * | 1999-02-19 | 2000-08-24 | Athersys, Inc. | Compositions and methods for non-targeted activation of endogenous genes |
-
1998
- 1998-09-25 WO PCT/US1998/020094 patent/WO1999015650A1/en not_active Ceased
- 1998-09-25 AT AT98949508T patent/ATE471373T1/de not_active IP Right Cessation
- 1998-09-25 JP JP2000512942A patent/JP5377806B2/ja not_active Expired - Lifetime
- 1998-09-25 BR BR9812540-0A patent/BR9812540A/pt not_active IP Right Cessation
- 1998-09-25 NZ NZ504185A patent/NZ504185A/xx unknown
- 1998-09-25 CN CN98811571A patent/CN100582230C/zh not_active Expired - Lifetime
- 1998-09-25 CA CA2304642A patent/CA2304642C/en not_active Expired - Lifetime
- 1998-09-25 EP EP98949508A patent/EP1017803B1/en not_active Expired - Lifetime
- 1998-09-25 DK DK98949508.0T patent/DK1017803T3/da active
- 1998-09-25 AU AU95816/98A patent/AU750025B2/en not_active Expired
- 1998-09-25 KR KR1020007003195A patent/KR20010024296A/ko not_active Ceased
- 1998-09-25 IL IL13524298A patent/IL135242A0/xx unknown
- 1998-09-25 DE DE69841723T patent/DE69841723D1/de not_active Expired - Lifetime
-
2000
- 2000-03-22 NO NO20001497A patent/NO20001497L/no not_active Application Discontinuation
- 2000-03-23 IL IL135242A patent/IL135242A/en not_active IP Right Cessation
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