JP2003516711A5 - - Google Patents
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- Publication number
- JP2003516711A5 JP2003516711A5 JP2000544768A JP2000544768A JP2003516711A5 JP 2003516711 A5 JP2003516711 A5 JP 2003516711A5 JP 2000544768 A JP2000544768 A JP 2000544768A JP 2000544768 A JP2000544768 A JP 2000544768A JP 2003516711 A5 JP2003516711 A5 JP 2003516711A5
- Authority
- JP
- Japan
- Prior art keywords
- medium
- keratinocytes
- culture
- cell line
- serum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000002510 keratinocyte Anatomy 0.000 description 25
- 239000002609 medium Substances 0.000 description 17
- 210000004027 cell Anatomy 0.000 description 9
- 238000000034 method Methods 0.000 description 7
- 210000003491 skin Anatomy 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 6
- 239000011248 coating agent Substances 0.000 description 5
- 238000000576 coating method Methods 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- 241000701806 Human papillomavirus Species 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 4
- 229940098773 bovine serum albumin Drugs 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- 102000012422 Collagen Type I Human genes 0.000 description 3
- 108010022452 Collagen Type I Proteins 0.000 description 3
- 102000016359 Fibronectins Human genes 0.000 description 3
- 108010067306 Fibronectins Proteins 0.000 description 3
- 108700020796 Oncogene Proteins 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- 230000001177 retroviral effect Effects 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 229940096422 collagen type i Drugs 0.000 description 2
- 210000000981 epithelium Anatomy 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000043276 Oncogene Human genes 0.000 description 1
- 206010040914 Skin reaction Diseases 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 210000002752 melanocyte Anatomy 0.000 description 1
- 231100001221 nontumorigenic Toxicity 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 231100000430 skin reaction Toxicity 0.000 description 1
- 230000035483 skin reaction Effects 0.000 description 1
- 210000000434 stratum corneum Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP98201247.8 | 1998-04-17 | ||
| EP98201247 | 1998-04-17 | ||
| PCT/EP1999/002347 WO1999054435A2 (fr) | 1998-04-17 | 1999-04-07 | Lignee de cellules immortalisees derivees de tissus cutanes humains normaux |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2003516711A JP2003516711A (ja) | 2003-05-20 |
| JP2003516711A5 true JP2003516711A5 (enExample) | 2009-10-22 |
| JP4671504B2 JP4671504B2 (ja) | 2011-04-20 |
Family
ID=8233616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000544768A Expired - Fee Related JP4671504B2 (ja) | 1998-04-17 | 1999-04-07 | 正常ヒト皮膚組織由来の不死化した細胞系 |
Country Status (22)
| Country | Link |
|---|---|
| US (1) | US6566136B2 (enExample) |
| EP (1) | EP1071747B1 (enExample) |
| JP (1) | JP4671504B2 (enExample) |
| KR (1) | KR20010074488A (enExample) |
| CN (1) | CN1299409A (enExample) |
| AT (1) | ATE274050T1 (enExample) |
| AU (1) | AU756151B2 (enExample) |
| BR (1) | BR9909699A (enExample) |
| CA (1) | CA2324479C (enExample) |
| CZ (1) | CZ20003842A3 (enExample) |
| DE (1) | DE69919531T2 (enExample) |
| DK (1) | DK1071747T3 (enExample) |
| ES (1) | ES2226383T3 (enExample) |
| HU (1) | HUP0101913A3 (enExample) |
| IL (1) | IL138417A0 (enExample) |
| NO (1) | NO325588B1 (enExample) |
| NZ (1) | NZ506944A (enExample) |
| PL (1) | PL201401B1 (enExample) |
| PT (1) | PT1071747E (enExample) |
| RU (1) | RU2244005C2 (enExample) |
| SK (1) | SK286870B6 (enExample) |
| WO (1) | WO1999054435A2 (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6368815B1 (en) * | 1999-03-29 | 2002-04-09 | Warner-Lambert Company | Screening of molecules that inhibit human phosphodiesterase 4A produced by non-recombinant cell lines |
| EP1130086A1 (en) * | 2000-02-18 | 2001-09-05 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Genetically engineered keratinocytes and toxicity assay using said keratinocytes |
| IT1316995B1 (it) * | 2000-02-25 | 2003-05-26 | Idi Irccs | Procedimento per ottenere da colture primarie di cheratinociti umanilinee cellulari immortalizzate. |
| US7262174B2 (en) | 2001-05-09 | 2007-08-28 | Geron Corporation | Treatment for wounds |
| DE10255285A1 (de) * | 2002-11-26 | 2004-06-03 | Mcs Micro Carrier Systems Gmbh | Selbst formende Phospholipid-Gele |
| US8669109B2 (en) | 2003-08-19 | 2014-03-11 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | Methods of producing proteins in Chinese hamster ovary (CHO) cells |
| DE10338531A1 (de) | 2003-08-19 | 2005-04-07 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | Verfahren zur Reklonierung von Produktionszellen |
| EP1557085A1 (en) | 2004-01-21 | 2005-07-27 | Boehringer Ingelheim International GmbH | Mouse model for human psoriasis |
| WO2006047426A2 (en) * | 2004-10-22 | 2006-05-04 | The Regents Of The University Of Michigan | Compositions, methods and systems for preparation of a stem cell-enriched cell population |
| CN101189326B (zh) * | 2004-12-23 | 2013-06-12 | 米迪缪尼有限公司 | 用于病毒增殖的非致瘤性mdck细胞系 |
| EP1739179A1 (en) * | 2005-06-30 | 2007-01-03 | Octapharma AG | Serum-free stable transfection and production of recombinant human proteins in human cell lines |
| CN105219697A (zh) * | 2014-07-04 | 2016-01-06 | 赫柏慧康生物科技无锡有限公司 | 一种人原代角质细胞分离制备的方法 |
| RU2573940C1 (ru) * | 2014-12-11 | 2016-01-27 | Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук | Клеточная линия рака почки человека ibgvat r 6 |
| RU2573938C1 (ru) * | 2014-12-11 | 2016-01-27 | Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук | Клеточная линия рака почки человека ibgvat r 27 |
| US12174172B2 (en) | 2019-12-19 | 2024-12-24 | Johnson & Johnson Consumer Inc. | Method for evaluating the efficacy of a composition in reducing the effects of cancer therapeutics on skin |
| WO2023123299A1 (en) * | 2021-12-31 | 2023-07-06 | Beijing Theraxyte Bioscience Co., Ltd. | Compositions and methods for culturing stem cells |
| CN115920011A (zh) * | 2022-11-25 | 2023-04-07 | 中科东方细胞科技有限公司 | 一种促进角质形成细胞增殖的组合物及其应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9210574D0 (en) * | 1992-05-18 | 1992-07-01 | Ca Nat Research Council | Biotherapeutic cell-coated microspheres for wound/burn and prothesis implant applications |
| HU222040B1 (hu) * | 1994-11-08 | 2003-04-28 | Cellfactors Plc | Eljárás humán porc, csont és neurális sejtvonalak előállítására |
| PT780469E (pt) * | 1995-12-21 | 2001-06-29 | Nestle Sa | Linhas imortalizadas de celulas derivadas de tecidos cutaneos humanos normais e meios de cultura sem soro adaptados a sua cultura |
| US5811297A (en) * | 1996-03-07 | 1998-09-22 | Amba Biosciences, Llc | Immortalized hematopoietic cell lines, cell system thereof with stromal cells, in vitro, ex vivo and in vivo uses, & in vitro generation of dendritic cells and macrophages |
-
1999
- 1999-04-07 EP EP99920605A patent/EP1071747B1/fr not_active Expired - Lifetime
- 1999-04-07 WO PCT/EP1999/002347 patent/WO1999054435A2/fr not_active Ceased
- 1999-04-07 DE DE69919531T patent/DE69919531T2/de not_active Expired - Lifetime
- 1999-04-07 RU RU2000129131/13A patent/RU2244005C2/ru not_active IP Right Cessation
- 1999-04-07 JP JP2000544768A patent/JP4671504B2/ja not_active Expired - Fee Related
- 1999-04-07 ES ES99920605T patent/ES2226383T3/es not_active Expired - Lifetime
- 1999-04-07 CN CN99805127A patent/CN1299409A/zh active Pending
- 1999-04-07 KR KR1020007011556A patent/KR20010074488A/ko not_active Abandoned
- 1999-04-07 PT PT99920605T patent/PT1071747E/pt unknown
- 1999-04-07 DK DK99920605T patent/DK1071747T3/da active
- 1999-04-07 CZ CZ20003842A patent/CZ20003842A3/cs unknown
- 1999-04-07 HU HU0101913A patent/HUP0101913A3/hu unknown
- 1999-04-07 PL PL364980A patent/PL201401B1/pl not_active IP Right Cessation
- 1999-04-07 CA CA002324479A patent/CA2324479C/en not_active Expired - Fee Related
- 1999-04-07 AT AT99920605T patent/ATE274050T1/de not_active IP Right Cessation
- 1999-04-07 BR BR9909699-4A patent/BR9909699A/pt not_active Application Discontinuation
- 1999-04-07 AU AU38135/99A patent/AU756151B2/en not_active Ceased
- 1999-04-07 NZ NZ506944A patent/NZ506944A/en unknown
- 1999-04-07 IL IL13841799A patent/IL138417A0/xx unknown
- 1999-04-07 SK SK1549-2000A patent/SK286870B6/sk not_active IP Right Cessation
-
2000
- 2000-09-18 US US09/663,645 patent/US6566136B2/en not_active Expired - Lifetime
- 2000-10-06 NO NO20005053A patent/NO325588B1/no not_active IP Right Cessation
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2003516711A5 (enExample) | ||
| Dlugosz et al. | [1] Isolation and utilization of epidermal keratinocytes for oncogene research | |
| Flores et al. | Establishment of the human papillomavirus type 16 (HPV-16) life cycle in an immortalized human foreskin keratinocyte cell line | |
| Reznikoff et al. | Growth and characterization of normal human urothelium in vitro | |
| US6485724B2 (en) | Immortalized human keratinocyte cell line | |
| Tsunenaga et al. | Growth and differentiation properties of normal and transformed human keratinocytes in organotypic culture | |
| JP4671504B2 (ja) | 正常ヒト皮膚組織由来の不死化した細胞系 | |
| Taichman et al. | The search for a culture system for papillomavirus | |
| Zhang et al. | Expansion and long-term culture of differentiated normal rat urothelial cells in vitro | |
| RU2000129131A (ru) | Иммортализованная клеточная линия, полученная из кожных тканей здорового человека | |
| US5786201A (en) | Human corneal epithelial cell lines with extended lifespan | |
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