JP2002543843A5 - - Google Patents
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- Publication number
- JP2002543843A5 JP2002543843A5 JP2000618463A JP2000618463A JP2002543843A5 JP 2002543843 A5 JP2002543843 A5 JP 2002543843A5 JP 2000618463 A JP2000618463 A JP 2000618463A JP 2000618463 A JP2000618463 A JP 2000618463A JP 2002543843 A5 JP2002543843 A5 JP 2002543843A5
- Authority
- JP
- Japan
- Prior art keywords
- propanediol
- pdd
- mutant
- blattae
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 201000007197 atypical autism Diseases 0.000 description 26
- 208000029560 autism spectrum disease Diseases 0.000 description 26
- 238000001407 pulse-discharge detection Methods 0.000 description 25
- 108010011958 1,3-propanediol dehydrogenase Proteins 0.000 description 18
- YPFDHNVEDLHUCE-UHFFFAOYSA-N 1,3-propanediol Substances OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 description 14
- DNIAPMSPPWPWGF-VKHMYHEASA-N (+)-propylene glycol Chemical compound C[C@H](O)CO DNIAPMSPPWPWGF-VKHMYHEASA-N 0.000 description 13
- 229920000166 polytrimethylene carbonate Polymers 0.000 description 13
- 241000588724 Escherichia coli Species 0.000 description 11
- 150000007523 nucleic acids Chemical class 0.000 description 9
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 230000035772 mutation Effects 0.000 description 7
- 108020004707 nucleic acids Proteins 0.000 description 7
- 102000039446 nucleic acids Human genes 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 125000003275 alpha amino acid group Chemical group 0.000 description 5
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 4
- 241000235648 Pichia Species 0.000 description 4
- 101150061843 dhaT gene Proteins 0.000 description 4
- 238000010367 cloning Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 108091033319 polynucleotide Proteins 0.000 description 3
- 102000040430 polynucleotide Human genes 0.000 description 3
- 239000002157 polynucleotide Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 241000228212 Aspergillus Species 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000588923 Citrobacter Species 0.000 description 2
- 241000193403 Clostridium Species 0.000 description 2
- 241000193469 Clostridium pasteurianum Species 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 241000235035 Debaryomyces Species 0.000 description 2
- 101710088194 Dehydrogenase Proteins 0.000 description 2
- 241000588914 Enterobacter Species 0.000 description 2
- 241000588722 Escherichia Species 0.000 description 2
- 241000588748 Klebsiella Species 0.000 description 2
- 241000588747 Klebsiella pneumoniae Species 0.000 description 2
- 241000235649 Kluyveromyces Species 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- 241000589350 Methylobacter Species 0.000 description 2
- 241000235395 Mucor Species 0.000 description 2
- SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- 241000235070 Saccharomyces Species 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 241000235346 Schizosaccharomyces Species 0.000 description 2
- 241000187747 Streptomyces Species 0.000 description 2
- 241000235017 Zygosaccharomyces Species 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 229940039696 lactobacillus Drugs 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical compound CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 108010025885 Glycerol dehydratase Proteins 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- UZMAPBJVXOGOFT-UHFFFAOYSA-N Syringetin Natural products COC1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UZMAPBJVXOGOFT-UHFFFAOYSA-N 0.000 description 1
- 239000007997 Tricine buffer Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000003889 chemical engineering Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical group [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13486899P | 1999-05-19 | 1999-05-19 | |
| US60/134,868 | 1999-05-19 | ||
| PCT/US2000/013286 WO2000070057A2 (en) | 1999-05-19 | 2000-05-16 | Mutant 1,3-propanediol dehydrogenase |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002543843A JP2002543843A (ja) | 2002-12-24 |
| JP2002543843A5 true JP2002543843A5 (https=) | 2010-10-07 |
| JP4646411B2 JP4646411B2 (ja) | 2011-03-09 |
Family
ID=22465381
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000618463A Expired - Lifetime JP4646411B2 (ja) | 1999-05-19 | 2000-05-16 | 変異体プロパンジオールデヒドロゲナーゼ |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US6468773B1 (https=) |
| EP (1) | EP1185667B1 (https=) |
| JP (1) | JP4646411B2 (https=) |
| CN (1) | CN1187448C (https=) |
| AT (1) | ATE485378T1 (https=) |
| AU (1) | AU777718B2 (https=) |
| BR (1) | BR0010752A (https=) |
| CA (1) | CA2373312C (https=) |
| DE (1) | DE60045125D1 (https=) |
| IL (1) | IL146172A0 (https=) |
| MX (1) | MXPA01011766A (https=) |
| WO (1) | WO2000070057A2 (https=) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1194559B1 (en) * | 1999-06-18 | 2006-09-20 | Research Foundation Of State University Of New York | Groups of borrelia burgdorferi that cause lyme disease in humans |
| WO2008052596A1 (en) * | 2006-10-31 | 2008-05-08 | Metabolic Explorer | Process for the biological production of n-butanol with high yield |
| WO2008052595A1 (en) * | 2006-10-31 | 2008-05-08 | Metabolic Explorer | Process for the biological production of 1,3-propanediol from glycerol with high yield |
| US20110229942A1 (en) * | 2007-12-13 | 2011-09-22 | Glycos Biotechnologies, Incorporated | Microbial Conversion of Oils and Fatty Acids to High-Value Chemicals |
| US9605283B2 (en) | 2012-07-11 | 2017-03-28 | Institut National Des Sciences Appliquées | Microorganism modified for the production of 1,3-propanediol |
| WO2015051298A2 (en) | 2013-10-04 | 2015-04-09 | Genomatica, Inc. | Alcohol dehydrogenase variants |
| CN103642765B (zh) * | 2013-12-25 | 2015-11-18 | 南京工业大学 | 醇脱氢酶突变体及其应用 |
| CN106609249B (zh) * | 2015-10-22 | 2019-07-23 | 中国科学院上海高等研究院 | 克雷伯氏肺炎杆菌突变菌及其生产1,3-丙二醇的应用 |
| US11441128B2 (en) | 2015-10-30 | 2022-09-13 | Genomatica, Inc. | Methanol dehydrogenase fusion proteins |
| CN116622657B (zh) * | 2023-07-17 | 2023-10-24 | 花安堂生物科技集团有限公司 | 一种1，3-丙二醇氧化还原酶突变体及含其融合蛋白与应用 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5686276A (en) * | 1995-05-12 | 1997-11-11 | E. I. Du Pont De Nemours And Company | Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism |
| ATE452979T1 (de) * | 1996-11-13 | 2010-01-15 | Du Pont | Herstellungsverfahren von 1,3-propandiol durch rekombinante organismen |
-
2000
- 2000-05-14 US US09/570,778 patent/US6468773B1/en not_active Expired - Lifetime
- 2000-05-16 CA CA2373312A patent/CA2373312C/en not_active Expired - Lifetime
- 2000-05-16 BR BR0010752-2A patent/BR0010752A/pt not_active IP Right Cessation
- 2000-05-16 AU AU50155/00A patent/AU777718B2/en not_active Expired
- 2000-05-16 AT AT00932432T patent/ATE485378T1/de not_active IP Right Cessation
- 2000-05-16 WO PCT/US2000/013286 patent/WO2000070057A2/en not_active Ceased
- 2000-05-16 DE DE60045125T patent/DE60045125D1/de not_active Expired - Lifetime
- 2000-05-16 IL IL14617200A patent/IL146172A0/xx unknown
- 2000-05-16 JP JP2000618463A patent/JP4646411B2/ja not_active Expired - Lifetime
- 2000-05-16 EP EP00932432A patent/EP1185667B1/en not_active Expired - Lifetime
- 2000-05-16 MX MXPA01011766A patent/MXPA01011766A/es active IP Right Grant
- 2000-05-16 CN CNB008077436A patent/CN1187448C/zh not_active Expired - Lifetime
-
2001
- 2001-11-16 US US09/991,138 patent/US6558933B2/en not_active Expired - Lifetime
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