JP2002543835A5 - - Google Patents
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- Publication number
- JP2002543835A5 JP2002543835A5 JP2000618446A JP2000618446A JP2002543835A5 JP 2002543835 A5 JP2002543835 A5 JP 2002543835A5 JP 2000618446 A JP2000618446 A JP 2000618446A JP 2000618446 A JP2000618446 A JP 2000618446A JP 2002543835 A5 JP2002543835 A5 JP 2002543835A5
- Authority
- JP
- Japan
- Prior art keywords
- magnetic particles
- complex
- solution
- cells
- silica
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000006249 magnetic particle Substances 0.000 description 43
- 238000000034 method Methods 0.000 description 29
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 22
- 210000004027 cell Anatomy 0.000 description 12
- 239000012620 biological material Substances 0.000 description 11
- 230000001419 dependent effect Effects 0.000 description 10
- 238000005342 ion exchange Methods 0.000 description 10
- 102000039446 nucleic acids Human genes 0.000 description 9
- 108020004707 nucleic acids Proteins 0.000 description 9
- 150000007523 nucleic acids Chemical class 0.000 description 9
- 239000000377 silicon dioxide Substances 0.000 description 9
- 239000006166 lysate Substances 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 239000013076 target substance Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- LRCFXGAMWKDGLA-UHFFFAOYSA-N dioxosilane;hydrate Chemical compound O.O=[Si]=O LRCFXGAMWKDGLA-UHFFFAOYSA-N 0.000 description 3
- 229960004029 silicic acid Drugs 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 239000013077 target material Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- -1 glycidyl-histidine modified silica Chemical class 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13415699P | 1999-05-14 | 1999-05-14 | |
| US60/134,156 | 1999-05-14 | ||
| PCT/US1999/031207 WO2000070040A1 (en) | 1999-05-14 | 1999-12-30 | Cell concentration and lysate clearance using paramagnetic particles |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009053541A Division JP2009118858A (ja) | 1999-05-14 | 2009-03-06 | 常磁性粒子を用いた集細胞及びライセート清澄化 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002543835A JP2002543835A (ja) | 2002-12-24 |
| JP2002543835A5 true JP2002543835A5 (enExample) | 2007-02-22 |
| JP4551568B2 JP4551568B2 (ja) | 2010-09-29 |
Family
ID=22462019
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000618446A Expired - Fee Related JP4551568B2 (ja) | 1999-05-14 | 1999-12-30 | 常磁性粒子を用いた集細胞及びライセート清澄化 |
| JP2009053541A Ceased JP2009118858A (ja) | 1999-05-14 | 2009-03-06 | 常磁性粒子を用いた集細胞及びライセート清澄化 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009053541A Ceased JP2009118858A (ja) | 1999-05-14 | 2009-03-06 | 常磁性粒子を用いた集細胞及びライセート清澄化 |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1179058B1 (enExample) |
| JP (2) | JP4551568B2 (enExample) |
| AT (2) | ATE521703T1 (enExample) |
| AU (1) | AU778486B2 (enExample) |
| CA (1) | CA2372485A1 (enExample) |
| WO (1) | WO2000070040A1 (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| US6914137B2 (en) | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| US7078224B1 (en) | 1999-05-14 | 2006-07-18 | Promega Corporation | Cell concentration and lysate clearance using paramagnetic particles |
| DE10033991A1 (de) * | 2000-07-12 | 2002-01-24 | Qiagen Gmbh | Verfahren zur Isolierung von Nukleinsäuren |
| ES2273915T3 (es) * | 2000-11-13 | 2007-05-16 | Promega Corporation | Depuracion de lisado y aislamiento de acido nucleico utilizando matrices de silica silanizadas. |
| AU2003224575A1 (en) * | 2002-05-02 | 2003-11-17 | Angiogenetics Sweden Ab | Isolation of target cells, capillaries and microorgans |
| US7601491B2 (en) | 2003-02-06 | 2009-10-13 | Becton, Dickinson And Company | Pretreatment method for extraction of nucleic acid from biological samples and kits therefor |
| US20070276131A1 (en) * | 2003-08-26 | 2007-11-29 | Danmarks Tekniske Universitet | Continuous Process for the Assembly of Macromolecular Substances and the Subsequent Capture and Isolation of Mamacromolecular Assembly and a System Suitable for the Process |
| DE10358137A1 (de) * | 2003-12-12 | 2005-07-07 | Merck Patent Gmbh | Verfahren und Kit zur Isolierung von RNA |
| CN100507549C (zh) | 2003-12-30 | 2009-07-01 | 3M创新有限公司 | 包括层的声传感器和涂渍该声传感器的方法 |
| US20060024776A1 (en) * | 2004-08-02 | 2006-02-02 | Mcmillian Ray | Magnetic particle capture of whole intact organisms from clinical samples |
| DE102005002343A1 (de) | 2005-01-18 | 2006-07-27 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Verfahren zur spezifischen oder unspezifischen Separation von Zellen und/oder Viren aus flüssigen Medien und dessen Verwendung |
| US20090311770A1 (en) * | 2005-05-20 | 2009-12-17 | Arkray, Inc. | Method of collecting microorganisms using fine particles, method of collecting nucleic acids using fine particles, and kits for use in the these methods |
| DE102008029356A1 (de) | 2008-06-20 | 2009-12-24 | Siemens Healthcare Diagnostics Gmbh | Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe |
| CN111690719A (zh) * | 2012-08-30 | 2020-09-22 | 凯杰有限公司 | 确定细胞样品中靶核酸存在或不存在的方法 |
| US20180135040A1 (en) | 2016-02-16 | 2018-05-17 | Life Magnetics, Inc. | Methods for separating nucleic acids with graphene coated magnetic beads |
| KR102047362B1 (ko) * | 2017-01-16 | 2019-11-21 | (주) 바이오팩트 | 아민-코팅 자성 나노입자를 이용한 플라스미드 dna 정제 방법 및 조성물 |
| US20220251539A1 (en) * | 2019-10-29 | 2022-08-11 | Hewlett-Packard Development Company, L.P. | Concentrating biological components |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5523231A (en) | 1990-02-13 | 1996-06-04 | Amersham International Plc | Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules |
| GB9003253D0 (en) | 1990-02-13 | 1990-04-11 | Amersham Int Plc | Precipitating polymers |
| US5395498A (en) | 1991-11-06 | 1995-03-07 | Gombinsky; Moshe | Method for separating biological macromolecules and means therfor |
| DE4307262A1 (de) * | 1993-03-02 | 1994-09-08 | Christian Bergemann | Magnetisches polymeres Siliciumdioxid |
| US5705628A (en) * | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
| US5652348A (en) | 1994-09-23 | 1997-07-29 | Massey University | Chromatographic resins and methods for using same |
| US5660984A (en) | 1994-12-09 | 1997-08-26 | Davis; Thomas E. | DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof |
| DE19512368A1 (de) * | 1995-04-01 | 1996-10-02 | Boehringer Mannheim Gmbh | System zur Freisetzung und Isolierung von Nukleinsäuren |
| EP0741141A2 (en) * | 1995-05-04 | 1996-11-06 | Hewlett-Packard Company | Method of purifying ologonucleotide from biological samples |
| US6027945A (en) * | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
-
1999
- 1999-12-30 EP EP99967755A patent/EP1179058B1/en not_active Expired - Lifetime
- 1999-12-30 JP JP2000618446A patent/JP4551568B2/ja not_active Expired - Fee Related
- 1999-12-30 AT AT99967755T patent/ATE521703T1/de not_active IP Right Cessation
- 1999-12-30 CA CA002372485A patent/CA2372485A1/en not_active Abandoned
- 1999-12-30 AT AT05017048T patent/ATE530648T1/de not_active IP Right Cessation
- 1999-12-30 WO PCT/US1999/031207 patent/WO2000070040A1/en not_active Ceased
- 1999-12-30 AU AU23981/00A patent/AU778486B2/en not_active Ceased
-
2009
- 2009-03-06 JP JP2009053541A patent/JP2009118858A/ja not_active Ceased
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