ATE521703T1 - Zellkonzentration und lysatklärung mittels paramagnetischen partikeln - Google Patents

Zellkonzentration und lysatklärung mittels paramagnetischen partikeln

Info

Publication number
ATE521703T1
ATE521703T1 AT99967755T AT99967755T ATE521703T1 AT E521703 T1 ATE521703 T1 AT E521703T1 AT 99967755 T AT99967755 T AT 99967755T AT 99967755 T AT99967755 T AT 99967755T AT E521703 T1 ATE521703 T1 AT E521703T1
Authority
AT
Austria
Prior art keywords
methods
disclosed
paramagnetic particles
present
cell concentration
Prior art date
Application number
AT99967755T
Other languages
English (en)
Inventor
Rex Bitner
Craig Smith
Jacqueline Sankbeil
Braeden Butler
Douglas White
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of ATE521703T1 publication Critical patent/ATE521703T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT99967755T 1999-05-14 1999-12-30 Zellkonzentration und lysatklärung mittels paramagnetischen partikeln ATE521703T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13415699P 1999-05-14 1999-05-14
PCT/US1999/031207 WO2000070040A1 (en) 1999-05-14 1999-12-30 Cell concentration and lysate clearance using paramagnetic particles

Publications (1)

Publication Number Publication Date
ATE521703T1 true ATE521703T1 (de) 2011-09-15

Family

ID=22462019

Family Applications (2)

Application Number Title Priority Date Filing Date
AT99967755T ATE521703T1 (de) 1999-05-14 1999-12-30 Zellkonzentration und lysatklärung mittels paramagnetischen partikeln
AT05017048T ATE530648T1 (de) 1999-05-14 1999-12-30 Zell konzentration und lysat klärung mittels paramagnetischen partikeln

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT05017048T ATE530648T1 (de) 1999-05-14 1999-12-30 Zell konzentration und lysat klärung mittels paramagnetischen partikeln

Country Status (6)

Country Link
EP (1) EP1179058B1 (de)
JP (2) JP4551568B2 (de)
AT (2) ATE521703T1 (de)
AU (1) AU778486B2 (de)
CA (1) CA2372485A1 (de)
WO (1) WO2000070040A1 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
DE10033991A1 (de) * 2000-07-12 2002-01-24 Qiagen Gmbh Verfahren zur Isolierung von Nukleinsäuren
AU2594202A (en) * 2000-11-13 2002-05-21 Promega Corp Lysate clearance and nucleic acid isolation using silanized silica matrices
US20060165662A1 (en) * 2002-05-02 2006-07-27 Minoru Takemoto Isolation of target cells, capillaries and microorgans
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
ATE405591T1 (de) * 2003-08-26 2008-09-15 Univ Danmarks Tekniske Kontinuierliches verfahren zur anordnung von makromolekulären substanzen und die anschliessende aufnahme und isolierung einer makromolekulären anordnung, sowie ein für dieses verfahren geeignetes system
DE10358137A1 (de) * 2003-12-12 2005-07-07 Merck Patent Gmbh Verfahren und Kit zur Isolierung von RNA
EP1711805A2 (de) 2003-12-30 2006-10-18 3M Innovative Properties Company Nachweiskassetten, -module, -systeme und -verfahren
US20060024776A1 (en) * 2004-08-02 2006-02-02 Mcmillian Ray Magnetic particle capture of whole intact organisms from clinical samples
DE102005002343A1 (de) 2005-01-18 2006-07-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zur spezifischen oder unspezifischen Separation von Zellen und/oder Viren aus flüssigen Medien und dessen Verwendung
WO2006123781A1 (ja) * 2005-05-20 2006-11-23 Arkray, Inc. 微粒子を用いた微生物及び核酸の回収方法ならびにそれらに用いるキット
DE102008029356A1 (de) 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe
CN104583423A (zh) * 2012-08-30 2015-04-29 凯杰有限公司 确定细胞样品中靶核酸存在或不存在的方法
US20180135040A1 (en) 2016-02-16 2018-05-17 Life Magnetics, Inc. Methods for separating nucleic acids with graphene coated magnetic beads
KR102047362B1 (ko) * 2017-01-16 2019-11-21 (주) 바이오팩트 아민-코팅 자성 나노입자를 이용한 플라스미드 dna 정제 방법 및 조성물
US20220251539A1 (en) * 2019-10-29 2022-08-11 Hewlett-Packard Development Company, L.P. Concentrating biological components

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5523231A (en) 1990-02-13 1996-06-04 Amersham International Plc Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules
GB9003253D0 (en) * 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
US5395498A (en) 1991-11-06 1995-03-07 Gombinsky; Moshe Method for separating biological macromolecules and means therfor
DE4307262A1 (de) 1993-03-02 1994-09-08 Christian Bergemann Magnetisches polymeres Siliciumdioxid
US5705628A (en) * 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5652348A (en) 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
US5660984A (en) 1994-12-09 1997-08-26 Davis; Thomas E. DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof
DE19512368A1 (de) * 1995-04-01 1996-10-02 Boehringer Mannheim Gmbh System zur Freisetzung und Isolierung von Nukleinsäuren
EP0741141A2 (de) * 1995-05-04 1996-11-06 Hewlett-Packard Company Verfahren zur Reinigung von Oligonucleoliden aus biologischen Proben
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles

Also Published As

Publication number Publication date
AU778486B2 (en) 2004-12-09
JP4551568B2 (ja) 2010-09-29
ATE530648T1 (de) 2011-11-15
JP2009118858A (ja) 2009-06-04
WO2000070040A1 (en) 2000-11-23
JP2002543835A (ja) 2002-12-24
AU2398100A (en) 2000-12-05
EP1179058B1 (de) 2011-08-24
CA2372485A1 (en) 2000-11-23
EP1179058A1 (de) 2002-02-13

Similar Documents

Publication Publication Date Title
ATE521703T1 (de) Zellkonzentration und lysatklärung mittels paramagnetischen partikeln
ATE266723T1 (de) Lösung die chaotropische mittel enthält und verwendung dieser lösung in verfahren zur isolierung von dna, rna und proteinen
ATE526019T1 (de) Verfahren zur behandlung von zellproliferationen störungen und virusinfektionen
Robertson et al. Double-stranded RNA as an inhibitor of protein synthesis and as a substrate for a nuclease in extracts of Krebs II ascites cells
DE60210621D1 (de) Universelle methode und zusammensetzung zur schnellen lysierung von zellen zur freisetzung von nukleinsäuren und ihre detektion
EA200101186A1 (ru) Регуляция экспрессии вирусных генов
CA2285918A1 (en) Methods for concentrating ligands using magnetic particles
DK0891420T3 (da) Fremgangsmåde til replikation af influenzavirus i cellekultur samt de ved fremgangsmåden opnåelige influenzavirus
WO2003020877A3 (en) Staining and sorting genomes and chromosomes using sequence-specific polyamides
DE60235005D1 (de) Nachweis der instabilität von mikrosatelliten und
SI1549735T1 (sl) KroĹľna DNA molekula, ki ima pogojni izvor podvojevanja, postopek za njeno pripravo in njena uporaba v genski terapiji
KR20240036522A (ko) RNA-가이드 CasΩ 뉴클레아제 및 진단 및 요법에서의 이의 용도
BRPI0615958B8 (pt) composição para aumentar a permeabilidade de paredes celulares e membranas celulares de mycobacterium sp. e método para detectar um alvo em uma célula
Kim et al. Purification and partial characterization of (6‐4) photoproduct DNA photolyase from Xenopus laevis
EP1135531A4 (de) Methoden zur introduktion heterologer zellen in fische
Chae et al. Genome edited Sirt1-overexpressing human mesenchymal stem cells exhibit therapeutic effects in treating collagen-induced arthritis
KRATZMEIER et al. Changes in the protein pattern of H1 histones associated with apoptotic DNA fragmentation
ES2196870T3 (es) Procedimiento para la purificacion o aislamiento de acidos nucleicos virales.
Dwivedi et al. Isolation and characterization of chromatin from Neurospora crassa
HRP20220722T1 (hr) Poboljšano hvatanje rnk interaktoma (eric)
ATE166395T1 (de) Für c.difficile spezifische oligonukleotide
ATE545708T1 (de) Krebsantigen mage-a9 und verwendungen davon
Liu et al. Mutational switching of a yeast tRNA synthetase into a mammalian-like synthetase cytokine
Zahn et al. Assessment of DNA-protein crosslinks in the course of aging in two mouse strains by use of a modified alkaline filter elution applied to whole tissue samples
Gadhia et al. Sodium fluoride induced chromosome aberrations and sister chromatid exchange in cultured human lymphocytes

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties