JP4551568B2 - 常磁性粒子を用いた集細胞及びライセート清澄化 - Google Patents

常磁性粒子を用いた集細胞及びライセート清澄化 Download PDF

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Publication number
JP4551568B2
JP4551568B2 JP2000618446A JP2000618446A JP4551568B2 JP 4551568 B2 JP4551568 B2 JP 4551568B2 JP 2000618446 A JP2000618446 A JP 2000618446A JP 2000618446 A JP2000618446 A JP 2000618446A JP 4551568 B2 JP4551568 B2 JP 4551568B2
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Prior art keywords
particles
solution
magnetic
tube
silica
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Expired - Fee Related
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JP2000618446A
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Japanese (ja)
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JP2002543835A (ja
JP2002543835A5 (enExample
Inventor
レックス エム ビットナー
クレイグ イー スミス
ジャクリーン サンクベイル
ブレイデン エル バトラー
ダグラス エイチ ホワイト
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Promega Corp
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Promega Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2000618446A 1999-05-14 1999-12-30 常磁性粒子を用いた集細胞及びライセート清澄化 Expired - Fee Related JP4551568B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13415699P 1999-05-14 1999-05-14
US60/134,156 1999-05-14
PCT/US1999/031207 WO2000070040A1 (en) 1999-05-14 1999-12-30 Cell concentration and lysate clearance using paramagnetic particles

Related Child Applications (1)

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JP2009053541A Division JP2009118858A (ja) 1999-05-14 2009-03-06 常磁性粒子を用いた集細胞及びライセート清澄化

Publications (3)

Publication Number Publication Date
JP2002543835A JP2002543835A (ja) 2002-12-24
JP2002543835A5 JP2002543835A5 (enExample) 2007-02-22
JP4551568B2 true JP4551568B2 (ja) 2010-09-29

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Family Applications (2)

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JP2000618446A Expired - Fee Related JP4551568B2 (ja) 1999-05-14 1999-12-30 常磁性粒子を用いた集細胞及びライセート清澄化
JP2009053541A Ceased JP2009118858A (ja) 1999-05-14 2009-03-06 常磁性粒子を用いた集細胞及びライセート清澄化

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JP2009053541A Ceased JP2009118858A (ja) 1999-05-14 2009-03-06 常磁性粒子を用いた集細胞及びライセート清澄化

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Country Link
EP (1) EP1179058B1 (enExample)
JP (2) JP4551568B2 (enExample)
AT (2) ATE530648T1 (enExample)
AU (1) AU778486B2 (enExample)
CA (1) CA2372485A1 (enExample)
WO (1) WO2000070040A1 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
DE10033991A1 (de) * 2000-07-12 2002-01-24 Qiagen Gmbh Verfahren zur Isolierung von Nukleinsäuren
CA2428532C (en) * 2000-11-13 2009-05-19 Promega Corporation Lysate clearance and nucleic acid isolation using silanized silica matrices
AU2003224575A1 (en) * 2002-05-02 2003-11-17 Angiogenetics Sweden Ab Isolation of target cells, capillaries and microorgans
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
ATE405591T1 (de) * 2003-08-26 2008-09-15 Univ Danmarks Tekniske Kontinuierliches verfahren zur anordnung von makromolekulären substanzen und die anschliessende aufnahme und isolierung einer makromolekulären anordnung, sowie ein für dieses verfahren geeignetes system
DE10358137A1 (de) * 2003-12-12 2005-07-07 Merck Patent Gmbh Verfahren und Kit zur Isolierung von RNA
AU2004315032A1 (en) 2003-12-30 2005-08-18 3M Innovative Properties Company Acousto-mechanical detection systems and methods of use
US20060024776A1 (en) * 2004-08-02 2006-02-02 Mcmillian Ray Magnetic particle capture of whole intact organisms from clinical samples
DE102005002343A1 (de) 2005-01-18 2006-07-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zur spezifischen oder unspezifischen Separation von Zellen und/oder Viren aus flüssigen Medien und dessen Verwendung
US20090311770A1 (en) * 2005-05-20 2009-12-17 Arkray, Inc. Method of collecting microorganisms using fine particles, method of collecting nucleic acids using fine particles, and kits for use in the these methods
DE102008029356A1 (de) 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe
EP2890809B1 (en) * 2012-08-30 2017-02-01 Qiagen GmbH Method of determining the presence or absence of a target nucleic acid in a cell sample
US20180135040A1 (en) 2016-02-16 2018-05-17 Life Magnetics, Inc. Methods for separating nucleic acids with graphene coated magnetic beads
KR102047362B1 (ko) * 2017-01-16 2019-11-21 (주) 바이오팩트 아민-코팅 자성 나노입자를 이용한 플라스미드 dna 정제 방법 및 조성물
WO2021086313A1 (en) * 2019-10-29 2021-05-06 Hewlett-Packard Development Company, L.P. Concentrating biological components

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5523231A (en) 1990-02-13 1996-06-04 Amersham International Plc Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules
GB9003253D0 (en) * 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
US5395498A (en) 1991-11-06 1995-03-07 Gombinsky; Moshe Method for separating biological macromolecules and means therfor
DE4307262A1 (de) 1993-03-02 1994-09-08 Christian Bergemann Magnetisches polymeres Siliciumdioxid
US5705628A (en) * 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5652348A (en) 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
US5660984A (en) 1994-12-09 1997-08-26 Davis; Thomas E. DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof
DE19512368A1 (de) * 1995-04-01 1996-10-02 Boehringer Mannheim Gmbh System zur Freisetzung und Isolierung von Nukleinsäuren
EP0741141A2 (en) * 1995-05-04 1996-11-06 Hewlett-Packard Company Method of purifying ologonucleotide from biological samples
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles

Also Published As

Publication number Publication date
ATE530648T1 (de) 2011-11-15
JP2009118858A (ja) 2009-06-04
CA2372485A1 (en) 2000-11-23
JP2002543835A (ja) 2002-12-24
EP1179058A1 (en) 2002-02-13
ATE521703T1 (de) 2011-09-15
EP1179058B1 (en) 2011-08-24
AU2398100A (en) 2000-12-05
WO2000070040A1 (en) 2000-11-23
AU778486B2 (en) 2004-12-09

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