CA2372485A1 - Cell concentration and lysate clearance using paramagnetic particles - Google Patents
Cell concentration and lysate clearance using paramagnetic particles Download PDFInfo
- Publication number
- CA2372485A1 CA2372485A1 CA002372485A CA2372485A CA2372485A1 CA 2372485 A1 CA2372485 A1 CA 2372485A1 CA 002372485 A CA002372485 A CA 002372485A CA 2372485 A CA2372485 A CA 2372485A CA 2372485 A1 CA2372485 A1 CA 2372485A1
- Authority
- CA
- Canada
- Prior art keywords
- magnetic particles
- particles
- solution
- magnetic
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000002245 particle Substances 0.000 title claims abstract description 170
- 239000006166 lysate Substances 0.000 title claims abstract description 78
- 230000005298 paramagnetic effect Effects 0.000 title abstract description 10
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- HHTGNFIMEJWAGT-MQWKRIRWSA-N (2s)-3-(1h-imidazol-5-yl)-2-(oxiran-2-ylmethylamino)propanoic acid Chemical compound C([C@@H](C(=O)O)NCC1OC1)C1=CNC=N1 HHTGNFIMEJWAGT-MQWKRIRWSA-N 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
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- JHIVVAPYMSGYDF-UHFFFAOYSA-N cyclohexanone Chemical compound O=C1CCCCC1 JHIVVAPYMSGYDF-UHFFFAOYSA-N 0.000 description 2
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- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
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- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
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- HSRJKNPTNIJEKV-UHFFFAOYSA-N Guaifenesin Chemical compound COC1=CC=CC=C1OCC(O)CO HSRJKNPTNIJEKV-UHFFFAOYSA-N 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-M Methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
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- 239000004793 Polystyrene Substances 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 239000004115 Sodium Silicate Substances 0.000 description 1
- 229910052770 Uranium Inorganic materials 0.000 description 1
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- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
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- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000012539 chromatography resin Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000005289 controlled pore glass Substances 0.000 description 1
- 238000012629 conventional elemental analysis Methods 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 230000005293 ferrimagnetic effect Effects 0.000 description 1
- 239000002902 ferrimagnetic material Substances 0.000 description 1
- 230000005294 ferromagnetic effect Effects 0.000 description 1
- 239000003302 ferromagnetic material Substances 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000001879 gelation Methods 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 125000003055 glycidyl group Chemical group C(C1CO1)* 0.000 description 1
- YQOKLYTXVFAUCW-UHFFFAOYSA-N guanidine;isothiocyanic acid Chemical compound N=C=S.NC(N)=N YQOKLYTXVFAUCW-UHFFFAOYSA-N 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 235000014304 histidine Nutrition 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000010841 mRNA extraction Methods 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 230000003169 placental effect Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000005373 porous glass Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 125000005372 silanol group Chemical group 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 description 1
- 229910052911 sodium silicate Inorganic materials 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
- LTOKKZDSYQQAHL-UHFFFAOYSA-N trimethoxy-[4-(oxiran-2-yl)butyl]silane Chemical compound CO[Si](OC)(OC)CCCCC1CO1 LTOKKZDSYQQAHL-UHFFFAOYSA-N 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13415699P | 1999-05-14 | 1999-05-14 | |
| US60/134,156 | 1999-05-14 | ||
| PCT/US1999/031207 WO2000070040A1 (en) | 1999-05-14 | 1999-12-30 | Cell concentration and lysate clearance using paramagnetic particles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2372485A1 true CA2372485A1 (en) | 2000-11-23 |
Family
ID=22462019
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002372485A Abandoned CA2372485A1 (en) | 1999-05-14 | 1999-12-30 | Cell concentration and lysate clearance using paramagnetic particles |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1179058B1 (enExample) |
| JP (2) | JP4551568B2 (enExample) |
| AT (2) | ATE530648T1 (enExample) |
| AU (1) | AU778486B2 (enExample) |
| CA (1) | CA2372485A1 (enExample) |
| WO (1) | WO2000070040A1 (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| US6914137B2 (en) | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| US7078224B1 (en) | 1999-05-14 | 2006-07-18 | Promega Corporation | Cell concentration and lysate clearance using paramagnetic particles |
| DE10033991A1 (de) * | 2000-07-12 | 2002-01-24 | Qiagen Gmbh | Verfahren zur Isolierung von Nukleinsäuren |
| CA2428532C (en) * | 2000-11-13 | 2009-05-19 | Promega Corporation | Lysate clearance and nucleic acid isolation using silanized silica matrices |
| AU2003224575A1 (en) * | 2002-05-02 | 2003-11-17 | Angiogenetics Sweden Ab | Isolation of target cells, capillaries and microorgans |
| US7601491B2 (en) | 2003-02-06 | 2009-10-13 | Becton, Dickinson And Company | Pretreatment method for extraction of nucleic acid from biological samples and kits therefor |
| ATE405591T1 (de) * | 2003-08-26 | 2008-09-15 | Univ Danmarks Tekniske | Kontinuierliches verfahren zur anordnung von makromolekulären substanzen und die anschliessende aufnahme und isolierung einer makromolekulären anordnung, sowie ein für dieses verfahren geeignetes system |
| DE10358137A1 (de) * | 2003-12-12 | 2005-07-07 | Merck Patent Gmbh | Verfahren und Kit zur Isolierung von RNA |
| AU2004315032A1 (en) | 2003-12-30 | 2005-08-18 | 3M Innovative Properties Company | Acousto-mechanical detection systems and methods of use |
| US20060024776A1 (en) * | 2004-08-02 | 2006-02-02 | Mcmillian Ray | Magnetic particle capture of whole intact organisms from clinical samples |
| DE102005002343A1 (de) | 2005-01-18 | 2006-07-27 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Verfahren zur spezifischen oder unspezifischen Separation von Zellen und/oder Viren aus flüssigen Medien und dessen Verwendung |
| US20090311770A1 (en) * | 2005-05-20 | 2009-12-17 | Arkray, Inc. | Method of collecting microorganisms using fine particles, method of collecting nucleic acids using fine particles, and kits for use in the these methods |
| DE102008029356A1 (de) | 2008-06-20 | 2009-12-24 | Siemens Healthcare Diagnostics Gmbh | Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe |
| EP2890809B1 (en) * | 2012-08-30 | 2017-02-01 | Qiagen GmbH | Method of determining the presence or absence of a target nucleic acid in a cell sample |
| US20180135040A1 (en) | 2016-02-16 | 2018-05-17 | Life Magnetics, Inc. | Methods for separating nucleic acids with graphene coated magnetic beads |
| KR102047362B1 (ko) * | 2017-01-16 | 2019-11-21 | (주) 바이오팩트 | 아민-코팅 자성 나노입자를 이용한 플라스미드 dna 정제 방법 및 조성물 |
| WO2021086313A1 (en) * | 2019-10-29 | 2021-05-06 | Hewlett-Packard Development Company, L.P. | Concentrating biological components |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5523231A (en) | 1990-02-13 | 1996-06-04 | Amersham International Plc | Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules |
| GB9003253D0 (en) * | 1990-02-13 | 1990-04-11 | Amersham Int Plc | Precipitating polymers |
| US5395498A (en) | 1991-11-06 | 1995-03-07 | Gombinsky; Moshe | Method for separating biological macromolecules and means therfor |
| DE4307262A1 (de) | 1993-03-02 | 1994-09-08 | Christian Bergemann | Magnetisches polymeres Siliciumdioxid |
| US5705628A (en) * | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
| US5652348A (en) | 1994-09-23 | 1997-07-29 | Massey University | Chromatographic resins and methods for using same |
| US5660984A (en) | 1994-12-09 | 1997-08-26 | Davis; Thomas E. | DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof |
| DE19512368A1 (de) * | 1995-04-01 | 1996-10-02 | Boehringer Mannheim Gmbh | System zur Freisetzung und Isolierung von Nukleinsäuren |
| EP0741141A2 (en) * | 1995-05-04 | 1996-11-06 | Hewlett-Packard Company | Method of purifying ologonucleotide from biological samples |
| US6027945A (en) * | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
-
1999
- 1999-12-30 CA CA002372485A patent/CA2372485A1/en not_active Abandoned
- 1999-12-30 AT AT05017048T patent/ATE530648T1/de not_active IP Right Cessation
- 1999-12-30 AT AT99967755T patent/ATE521703T1/de not_active IP Right Cessation
- 1999-12-30 AU AU23981/00A patent/AU778486B2/en not_active Ceased
- 1999-12-30 EP EP99967755A patent/EP1179058B1/en not_active Expired - Lifetime
- 1999-12-30 JP JP2000618446A patent/JP4551568B2/ja not_active Expired - Fee Related
- 1999-12-30 WO PCT/US1999/031207 patent/WO2000070040A1/en not_active Ceased
-
2009
- 2009-03-06 JP JP2009053541A patent/JP2009118858A/ja not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| ATE530648T1 (de) | 2011-11-15 |
| JP2009118858A (ja) | 2009-06-04 |
| JP2002543835A (ja) | 2002-12-24 |
| EP1179058A1 (en) | 2002-02-13 |
| ATE521703T1 (de) | 2011-09-15 |
| EP1179058B1 (en) | 2011-08-24 |
| AU2398100A (en) | 2000-12-05 |
| JP4551568B2 (ja) | 2010-09-29 |
| WO2000070040A1 (en) | 2000-11-23 |
| AU778486B2 (en) | 2004-12-09 |
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| Date | Code | Title | Description |
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| EEER | Examination request | ||
| FZDE | Discontinued |