JP2001521765A - 特異的かつ高感度な核酸検出方法 - Google Patents

特異的かつ高感度な核酸検出方法

Info

Publication number
JP2001521765A
JP2001521765A JP2000519599A JP2000519599A JP2001521765A JP 2001521765 A JP2001521765 A JP 2001521765A JP 2000519599 A JP2000519599 A JP 2000519599A JP 2000519599 A JP2000519599 A JP 2000519599A JP 2001521765 A JP2001521765 A JP 2001521765A
Authority
JP
Japan
Prior art keywords
nucleic acid
probe
sequence
binding
detected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2000519599A
Other languages
English (en)
Japanese (ja)
Other versions
JP2001521765A5 (enExample
Inventor
ケスラー，クリストフ
ハーベルハウゼン，ゲルト
バルトル，クヌート
オルム，ヘンリック
Original Assignee
ロシュ ダイアグノスティックス ゲーエムベーハー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19748690A external-priority patent/DE19748690A1/de
Priority claimed from DE19814001A external-priority patent/DE19814001A1/de
Priority claimed from DE19814828A external-priority patent/DE19814828A1/de
Application filed by ロシュ ダイアグノスティックス ゲーエムベーハー filed Critical ロシュ ダイアグノスティックス ゲーエムベーハー
Publication of JP2001521765A publication Critical patent/JP2001521765A/ja
Publication of JP2001521765A5 publication Critical patent/JP2001521765A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2000519599A 1997-11-04 1998-11-03 特異的かつ高感度な核酸検出方法 Pending JP2001521765A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
DE19748690A DE19748690A1 (de) 1997-11-04 1997-11-04 Spezifisches und sensitives Nukleinsäurenachweisverfahren
DE19814001A DE19814001A1 (de) 1998-03-28 1998-03-28 Spezifisches und sensitives Nukleinsäurenachweisverfahren
DE19814828.3 1998-04-02
DE19748690.8 1998-04-02
DE19814001.0 1998-04-02
DE19814828A DE19814828A1 (de) 1998-04-02 1998-04-02 Spezifisches und sensitives Nukleinsäurenachweisverfahren
PCT/EP1998/006951 WO1999024606A2 (de) 1997-11-04 1998-11-03 Spezifisches und sensitives nukleinsäurenachweisverfahren

Publications (2)

Publication Number Publication Date
JP2001521765A true JP2001521765A (ja) 2001-11-13
JP2001521765A5 JP2001521765A5 (enExample) 2006-01-05

Family

ID=27217887

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2000519599A Pending JP2001521765A (ja) 1997-11-04 1998-11-03 特異的かつ高感度な核酸検出方法
JP2000519104A Pending JP2002509694A (ja) 1997-11-04 1998-11-03 特異的かつ高感度な核酸検出方法
JP2000519105A Pending JP2002505071A (ja) 1997-11-04 1998-11-03 特異的かつ高感度な核酸検出方法

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2000519104A Pending JP2002509694A (ja) 1997-11-04 1998-11-03 特異的かつ高感度な核酸検出方法
JP2000519105A Pending JP2002505071A (ja) 1997-11-04 1998-11-03 特異的かつ高感度な核酸検出方法

Country Status (6)

Country Link
US (1) US7105318B2 (enExample)
EP (3) EP1029084A2 (enExample)
JP (3) JP2001521765A (enExample)
AU (3) AU1232099A (enExample)
CA (3) CA2308762A1 (enExample)
WO (3) WO1999023250A2 (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
CA2313641A1 (en) * 1997-12-12 1999-06-24 Digene Corporation Universal collection medium
WO1999049293A2 (en) * 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) * 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US7807802B2 (en) 2002-11-12 2010-10-05 Abbott Lab Polynucleotides for the amplification and detection of Chlamydia trachomatis and Neisseria gonorrhoeae
EP3095873B1 (en) 2006-12-21 2018-04-18 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
EP2121984A2 (en) * 2007-03-16 2009-11-25 454 Life Sciences Corporation System and method for detection of hiv drug resistant variants
GB0714389D0 (en) 2007-07-21 2007-09-05 Barry Callebaut Ag Process and product
JP2011518333A (ja) * 2008-04-17 2011-06-23 キアジェン ゲイサーズバーグ インコーポレイテッド 標的核酸の存在を判別するための合成プローブを用いた組成物、方法、およびキット
TWI419974B (zh) 2008-10-27 2013-12-21 Qiagen Gaithersburg Inc 於自動平台上之快速結果雜交捕捉法
WO2010088292A1 (en) * 2009-01-28 2010-08-05 Qiagen Gaithersburg, Inc. Sequence-specific large volume sample preparation method and assay
AU2010242867B2 (en) * 2009-05-01 2016-05-12 Qiagen Gaithersburg, Inc. A non-target amplification method for detection of RNA splice-forms in a sample
CA2766391C (en) 2009-07-01 2021-04-20 Gen-Probe Incorporated Methods and compositions for multiplex nucleic acid amplification
EP2478087B1 (en) 2009-09-14 2017-01-18 QIAGEN Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
JP2013517803A (ja) 2010-01-29 2013-05-20 キアジェン ゲイサーズバーグ インコーポレイテッド サンプル中のｈｐｖの存在を決定および確認する方法
WO2011094514A1 (en) 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
EP2572001A2 (en) 2010-05-19 2013-03-27 QIAGEN Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
CA2799205A1 (en) 2010-05-25 2011-12-01 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically-truncated probes
EP2678442B1 (en) 2011-02-24 2019-01-16 QIAGEN Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acid
JP2024097096A (ja) * 2021-04-28 2024-07-18 バイオニクス株式会社 光学測定装置

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1190838A (en) * 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US5200313A (en) 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
AU606043B2 (en) * 1985-03-28 1991-01-31 F. Hoffmann-La Roche Ag Detection of viruses by amplification and hybridization
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
ES286841Y (es) 1985-05-20 1988-10-01 Vileda Gmbh Dispositivo de acoplamiento de un mango a un util de limpieza
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5639871A (en) * 1988-09-09 1997-06-17 Roche Molecular Systems, Inc. Detection of human papillomavirus by the polymerase chain reaction
WO1991001384A1 (en) 1989-07-11 1991-02-07 Gen-Probe, Incorporated Nucleic acid sequence amplification methods utilizing a transcription complex
NL9000134A (nl) * 1990-01-19 1991-08-16 Stichting Res Fonds Pathologie Primers en werkwijze voor het detecteren van humaan papilloma virus genotypen m.b.v. pcr.
US5453355A (en) * 1990-01-26 1995-09-26 Abbott Laboratories Oligonucleotides and methods for the detection of Neisseria gonorrhoeae
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
RU2155228C2 (ru) * 1991-05-08 2000-08-27 Чирон Корпорейшн Hcv геномные последовательности для диагностических и терапевтических целей
DK0529493T3 (da) 1991-08-27 1998-08-24 Hoffmann La Roche Fremgangsmåder og reagenser til hepatitis C-detektion
JPH05308999A (ja) * 1992-05-08 1993-11-22 Sumitomo Metal Ind Ltd Ｂ型肝炎ウイルスｐｒｅ−Ｃ変異の判定法
WO1994003635A1 (en) 1992-08-04 1994-02-17 Institute Of Molecular Biology & Biotechnology Rapid amplification and detection of nucleic acids
US5605798A (en) * 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
EP0785996A1 (en) * 1993-07-13 1997-07-30 Abbott Laboratories Nucleotide sequences and process for amplifying and detection of hepatitis b virus
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
AT406019B (de) * 1995-05-08 2000-01-25 Immuno Ag Verfahren zur herstellung eines arzneimittels enthaltend ein oder mehrere plasmaderivate
US5882857A (en) 1995-06-07 1999-03-16 Behringwerke Ag Internal positive controls for nucleic acid amplification
DE69739357D1 (de) 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6333179B1 (en) * 1997-06-20 2001-12-25 Affymetrix, Inc. Methods and compositions for multiplex amplification of nucleic acids
WO1999040226A2 (en) 1998-02-04 1999-08-12 Perkin-Elmer Corporation Determination of a genotype of an amplification product at multiple allelic sites
US5948648A (en) 1998-05-29 1999-09-07 Khan; Shaheer H. Nucleotide compounds including a rigid linker

Also Published As

Publication number Publication date
AU2152199A (en) 1999-05-24
US7105318B2 (en) 2006-09-12
CA2312779A1 (en) 1999-05-20
JP2002505071A (ja) 2002-02-19
AU1232099A (en) 1999-05-24
AU741141B2 (en) 2001-11-22
US20030175765A1 (en) 2003-09-18
WO1999023249A2 (de) 1999-05-14
EP1029084A2 (de) 2000-08-23
EP1029077A2 (de) 2000-08-23
WO1999023250A2 (de) 1999-05-14
WO1999024606A2 (de) 1999-05-20
CA2308762A1 (en) 1999-05-14
CA2308368C (en) 2009-01-20
WO1999023250A3 (de) 1999-07-22
WO1999023249A3 (de) 1999-09-10
CA2308368A1 (en) 1999-05-14
JP2002509694A (ja) 2002-04-02
EP1029083A2 (de) 2000-08-23
AU2152099A (en) 1999-05-31
WO1999024606A3 (de) 1999-07-22

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