JP2000511043A - ノッチ経路を用いる最終未分化細胞の操作 - Google Patents
ノッチ経路を用いる最終未分化細胞の操作Info
- Publication number
- JP2000511043A JP2000511043A JP09513747A JP51374797A JP2000511043A JP 2000511043 A JP2000511043 A JP 2000511043A JP 09513747 A JP09513747 A JP 09513747A JP 51374797 A JP51374797 A JP 51374797A JP 2000511043 A JP2000511043 A JP 2000511043A
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- cells
- notch
- cell
- progenitor cells
- progenitor
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Abstract
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Claims (1)
- 【特許請求の範囲】 1.ヒト前駆細胞を増殖させる方法であって、該細胞の分化を抑制するのに有 効な量のノッチ(Notch)機能のアゴニストと該細胞とを接触させ、該細胞を細胞 増殖条件にさらして該細胞を増殖させることを含んでなる方法。 2.該前駆細胞が外胚葉に由来する、請求項1に記載の方法。 3.該前駆細胞が内胚葉に由来する、請求項1に記載の方法。 4.該前駆細胞が中胚葉に由来する、請求項1に記載の方法。 5.該前駆細胞が、造血前駆細胞、上皮前駆細胞、腎前駆細胞、神経前駆細胞 、皮膚前駆細胞、造骨細胞前駆細胞、軟骨細胞前駆細胞および肝前駆細胞よりな る群から選ばれる、請求項1に記載の方法。 6.該アゴニストが、デルタ(Delta)タンパク質であるか、またはノッチに 結合するその誘導体である、請求項1に記載の方法。 7.該アゴニストが、セレイト(Serrate)タンパク質であるか、またはノッ チに結合するその誘導体である、請求項1に記載の方法。 8.該アゴニストが、ノッチ(Notch)タンパク質に対する抗体であるか、また は該結合領域を含有する該抗体の断片である、請求項1に記載の方法。 9.該前駆細胞が造血幹細胞である、請求項1に記載の方法。 10.該前駆細胞が、ヒトの疾患または障害の治療において重要なタンパク質を コードする組換え核酸を含有する、請求項1に記載の方法。 11.該アゴニストがデルタ(Delta)またはセレイト(Serrate)タンパク質で あり、該接触を、該アゴニストを組換え的に発現する細胞に該前駆細胞をさらす ことを含む方法により行なう、請求項1に記載の方法。 12.該接触を、精製された可溶性型のアゴニストを含有する培地中で該前駆細 胞を培養することにより行なう、請求項1に記載の方法。 13.該細胞の分化が実質的に全く生じない、請求項1に記載の方法。 14.前駆細胞を増殖させる方法であって、該細胞の分化を抑制するのに有効な 量のノッチ機能の可溶性アゴニストと該細胞とを接触させ、該細胞を細胞増殖条 件にさらして該細胞を増殖させることを含んでなる方法。 15.該前駆細胞が、造血前駆細胞、上皮前駆細胞、腎前駆細胞、神経前駆細胞 、皮膚前駆細胞、造骨細胞前駆細胞、軟骨細胞前駆細胞、肝前駆細胞および筋細 胞よりなる群から選ばれる、請求項14に記載の方法。 16.該前駆細胞が造血幹細胞である、請求項14に記載の方法。 17.該細胞の分化が実質的に全く生じない、請求項14に記載の方法。 18.該可溶性アゴニストが、ノッチ(Notch)タンパク質に結合するデルタ(Del ta)タンパク質の誘導体である、請求項14に記載の方法。 19.該可溶性アゴニストが、ノッチ(Notch)タンパク質に結合するセレイト(S errate)タンパク質の誘導体である、請求項14に記載の方法。 20.前記のデルタ(Delta)の誘導体が、デルタタンパク質の細胞外ドメイン より実質的になる、請求項18に記載の方法。 21.前記のセレイト(Serrate)の誘導体が、セレイトタンパク質の細胞外ド メインより本質的になる、請求項19に記載の方法。 22.該可溶性アゴニストが、ノッチ(Notdl)タンパク質に対する抗体であるか 、または該結合領域を含有する該抗体の断片である、請求項14に記載の方法。 23.前駆細胞を増殖させる方法であって、該前駆細胞においてノッチ(Notch) タンパク質に結合するデルテックス(Deltex)タンパク質またはその断片を、該 細胞の分化を抑制するのに有効な量にて該細胞内で組換え的に発現させ、該細胞 を細胞増殖条件にさらして該細胞を増殖させることを含んでなる方法。 24.造血前駆細胞を増殖させる方法であって、該前駆細胞内のノッチタンパク 質の細胞内ドメインより本質的になるノッチ(Notch)タンパク質を、分化を抑制 するのに有効な量にて該細胞内で組換え的に発現させ、該細胞を細胞増殖条件に さらして該細胞を増殖させることを含んでなる方法。 25.上皮前駆細胞を増殖させる方法であって、該前駆細胞内のノッチタンパク 質の細胞内ドメインより本質的になるノッチタンパク質を、分化を抑制するのに 有効な量にて該細胞内で組換え的に発現させ、該細胞を細胞増殖条件にさらして 該細胞を増殖させることを含んでなる方法。 26.肝前駆細胞を増殖させる方法であって、該前駆細胞内のノッチタンパク質 の細胞内ドメインより本質的になるノッチタンパク質を、分化を抑制するのに有 効な量にて該細 胞内で組換え的に発現させ、該細胞を細胞増殖条件にさらして該細胞を増殖させ ることを含んでなる方法。 27.ヒト前駆細胞を増殖させる方法であって、該前駆細胞を第2の細胞と接触 させ、該前駆細胞を細胞増殖条件にさらして該前駆細胞を増殖させることを含ん でなり、前記の第2の細胞が、その表面上で、少なくともノッチのリガンドの細 胞外ドメインよりなる分子を組換え的に発現することを特徴とする方法。 28.前記の第2の細胞が、その表面上で、少なくともデル(Delta)タンパク 質の細胞外ドメインを組換え的に発現する、請求項27に記載の方法。 29.前記の第2の細胞が、その表面上で、少なくともセレイト(Serrate)タ ンパク質の細胞外ドメインを組換え的に発現する、請求項27に記載の方法。 30.造血前駆細胞を増殖させる方法であって、該前駆細胞を第2の細胞と接触 させ、該前駆細胞を細胞増殖条件にさらして該前駆細胞を増殖させることを含ん でなり、前記の第2の細胞が、その表面上で、少なくともノッチのリガンドの細 胞外ドメインよりなる分子を組換え的に発現することを特徴とする方法。 31.該前駆細胞が、疾患または障害の治療において重要なタンパク質をコード する組換え核酸を含有する、請求項14に記載の方法。 32.前記の接触工程の後に、疾患または障害の治療において重要なタンパク質 をコードする組換え核酸を該細胞内に導入する工程をさらに含んでなる、請求項 14に記載の方法。 33.ヒト前駆細胞を増殖させる方法であって、該細胞の分化を抑制するのに有 効な量の、ノッチのリガンドを発現する第2の細胞と、該前駆細胞とを接触させ 、該前駆細胞を細胞増殖条件にさらして該前駆細胞を増殖させることを含んでな る方法。 34.前駆細胞の分化を抑制するのに有効な量のノッチ機能のアゴニストと該前 駆細胞とを接触させ、該細胞を細胞増殖条件にさらして、増殖された前駆細胞集 団を形成させ、その増殖された前駆細胞集団またはそれから産生された子孫細胞 の治療的に有効な量を患者に投与することを含んでなる治療方法。 35.前記のノッチ機能のアゴニストを除去し、得られた増殖された細胞の少な くともいくつかの分化を誘導することをさらに含んでなる、請求項lまたは14に 記載の方法。 36.細胞の増殖または分化を調節するシグナル伝達経路の機能を抑制する方法 であっ て、細胞の増殖または分化を調節する該細胞内のシグナル伝達経路の機能を抑制 するのに有効な量のノッチ機能のアゴニストと該細胞とを接触させることを含ん でなる方法。 37.該経路が、rasに媒介される経路である、請求項36に記載の方法。 38.該経路が、wnt-1または相同座に媒介される経路である、請求項36に記載 の方法。 39.前記の接触させる工程とさらす工程とを同時に行なう、請求項1に記載の方 法。 40.前記の接触させる工程とさらす工程とをin vitroで行なう、請求項1、14 または16に記載の方法。 41.哺乳動物のニューロン細胞の増殖を促進する方法であって、哺乳動物のニ ューロンをノッチ機能のアンタゴニストと接触させ、該ニューロンをニューロン 細胞の増殖条件にさらすことを含んでなる方法。 42.哺乳動物の円柱上皮細胞の増殖を促進する方法であって、頚円柱上皮細胞 をノッチ機能のアンタゴニストと接触させ、該上皮細胞を上皮細胞の増殖条件に さらすことを含んでなる方法。 43.ノッチを発現する成熟細胞、または抗ノッチ抗体が免疫特異的に結合しう るノッチの断片もしくは誘導体を発現する成熟細胞の増殖を促進する方法であっ て、ノッチを発現する成熟細胞、または抗ノッチ抗体が免疫特異的に結合しうる ノッチの断片もしくは誘導体を発現する成熟細胞を、ノッチ機能のアンタゴニス トと接触させ、該細胞を細胞増殖条件にさらすことを含んでなる方法。
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US08/537,210 US5780300A (en) | 1995-09-29 | 1995-09-29 | Manipulation of non-terminally differentiated cells using the notch pathway |
PCT/US1996/015651 WO1997011716A1 (en) | 1995-09-29 | 1996-09-27 | Manipulation of non-terminally differentiated cells using the notch pathway |
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WO2015004762A1 (ja) * | 2013-07-10 | 2015-01-15 | 株式会社日立製作所 | 細胞培養方法、装置、及び細胞シート |
JP2020072694A (ja) * | 2014-06-04 | 2020-05-14 | フレッド ハッチンソン キャンサー リサーチ センター | Notch 1および/またはNotch 2アゴニストを用いた幹細胞の増殖および生着 |
JP7121720B2 (ja) | 2014-06-04 | 2022-08-18 | フレッド ハッチンソン キャンサー リサーチ センター | Notch 1および/またはNotch 2アゴニストを用いた幹細胞の増殖および生着 |
JP2018506293A (ja) * | 2015-02-24 | 2018-03-08 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 結合誘発型転写スイッチ及びその使用方法 |
JP2021019625A (ja) * | 2015-02-24 | 2021-02-18 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 結合誘発型転写スイッチ及びその使用方法 |
JP2022145748A (ja) * | 2015-02-24 | 2022-10-04 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 結合誘発型転写スイッチ及びその使用方法 |
JP7357731B2 (ja) | 2015-02-24 | 2023-10-06 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 結合誘発型転写スイッチ及びその使用方法 |
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WO1997011716A1 (en) | 1997-04-03 |
AU7264996A (en) | 1997-04-17 |
US20040058443A1 (en) | 2004-03-25 |
EP0948348A4 (en) | 2002-05-22 |
JP2009143934A (ja) | 2009-07-02 |
EP0948348B1 (en) | 2014-01-01 |
AU732629B2 (en) | 2001-04-26 |
EP0948348A1 (en) | 1999-10-13 |
CA2233534A1 (en) | 1997-04-03 |
US5780300A (en) | 1998-07-14 |
US6149902A (en) | 2000-11-21 |
CA2233534C (en) | 2012-06-05 |
US20070166824A1 (en) | 2007-07-19 |
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