IN2014DN07878A - - Google Patents
Info
- Publication number
- IN2014DN07878A IN2014DN07878A IN7878DEN2014A IN2014DN07878A IN 2014DN07878 A IN2014DN07878 A IN 2014DN07878A IN 7878DEN2014 A IN7878DEN2014 A IN 7878DEN2014A IN 2014DN07878 A IN2014DN07878 A IN 2014DN07878A
- Authority
- IN
- India
- Prior art keywords
- relates
- methods
- primer extension
- polymerase lacking
- detection
- Prior art date
Links
- 238000000034 method Methods 0.000 abstract 4
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 2
- 108060002716 Exonuclease Proteins 0.000 abstract 1
- 101710163270 Nuclease Proteins 0.000 abstract 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical group OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000000539 dimer Substances 0.000 abstract 1
- 102000013165 exonuclease Human genes 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/GB2012/050645 WO2013140107A1 (en) | 2012-03-22 | 2012-03-22 | Polymerase chain reaction detection system using oligonucleotides comprising a phosphorothioate group |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IN2014DN07878A true IN2014DN07878A (enrdf_load_stackoverflow) | 2015-04-24 |
Family
ID=45894595
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IN7878DEN2014 IN2014DN07878A (enrdf_load_stackoverflow) | 2012-03-22 | 2012-03-22 |
Country Status (18)
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2515990B (en) * | 2013-03-06 | 2016-05-04 | Lgc Genomics Ltd | Polymerase chain reaction detection system |
| CN109097456A (zh) * | 2014-11-28 | 2018-12-28 | 深圳市海普洛斯生物科技有限公司 | 单分子高保真扩增方法 |
| CN107208160B (zh) | 2015-01-30 | 2023-02-17 | 哈佛学院院长及董事 | 无显微镜成像 |
| CN116949140A (zh) | 2016-02-17 | 2023-10-27 | 哈佛学院院长及董事 | 分子编程工具 |
| US11359229B2 (en) | 2016-09-20 | 2022-06-14 | President And Fellows Of Harvard College | Molecular verification systems |
| CN106755379B (zh) * | 2016-12-13 | 2021-06-04 | 海南医学院 | 对4种曲霉菌同步定量和基因分型的二聚体突变荧光引物定量pcr方法 |
| RU2698134C2 (ru) | 2017-12-04 | 2019-08-22 | Общество с ограниченной ответственностью "Биолабмикс" | Способ амплификации нуклеиновых кислот с использованием фосфорилгуанидиновых олигонуклеотидов |
| GB201713251D0 (en) * | 2017-08-18 | 2017-10-04 | Lgc Genomics Ltd | Methods and kits for detection of nucleic acid molecules |
| KR102380264B1 (ko) * | 2017-08-31 | 2022-03-29 | 주식회사 씨젠 | 다이머-형성 프라이머 쌍을 이용한 구성요소의 성능 평가 |
| CN111683897A (zh) * | 2017-11-03 | 2020-09-18 | 华盛顿大学 | 使用编码颗粒的数字核酸扩增 |
| EP3768852B1 (en) | 2018-03-22 | 2023-08-30 | President and Fellows of Harvard College | Methods and compositions for molecular authentication |
| CN108715888A (zh) * | 2018-06-07 | 2018-10-30 | 王卫东 | 一种基于fret的pcr均相检测系统及其应用 |
| CN112342213B (zh) * | 2020-10-26 | 2022-10-11 | 清华大学 | 核酸分子及其应用 |
| CN113846187B (zh) * | 2021-10-24 | 2023-07-18 | 北京宏微特斯生物科技有限公司 | 利用荧光探针熔解曲线检测待测目标核酸序列的方法及其试剂盒 |
| CN114250281B (zh) * | 2021-11-03 | 2024-12-17 | 深圳铭毅智造科技有限公司 | 一种核酸代谢酶活性检测方法 |
| CN116973467A (zh) * | 2022-04-29 | 2023-10-31 | 南京金斯瑞生物科技有限公司 | 一种经硫代磷酸化修饰的核酸序列的修饰鉴定方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5516633A (en) * | 1991-08-15 | 1996-05-14 | Amersham Life Science, Inc. | DNA sequencing with a T7-type gene 6 exonuclease |
| CA2176193A1 (en) * | 1995-05-22 | 1996-11-23 | John Wesley Backus | Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers |
| US6727356B1 (en) | 1999-12-08 | 2004-04-27 | Epoch Pharmaceuticals, Inc. | Fluorescent quenching detection reagents and methods |
| US7019129B1 (en) | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
| GB0510979D0 (en) | 2005-05-28 | 2005-07-06 | Kbiosciences Ltd | Detection system for PCR assay |
| AT502823B1 (de) * | 2005-11-29 | 2007-06-15 | Seitz Alexander Dr | Polynukleotid-amplifikation |
-
2012
- 2012-03-22 CN CN201280073407.6A patent/CN104379763B/zh active Active
- 2012-03-22 AU AU2012374566A patent/AU2012374566B2/en active Active
- 2012-03-22 IN IN7878DEN2014 patent/IN2014DN07878A/en unknown
- 2012-03-22 EP EP12710994.0A patent/EP2828399B1/en active Active
- 2012-03-22 RS RS20170376A patent/RS55868B1/sr unknown
- 2012-03-22 HR HRP20170569TT patent/HRP20170569T1/hr unknown
- 2012-03-22 ES ES12710994.0T patent/ES2620750T3/es active Active
- 2012-03-22 PT PT127109940T patent/PT2828399T/pt unknown
- 2012-03-22 DK DK12710994.0T patent/DK2828399T3/en active
- 2012-03-22 CA CA2867916A patent/CA2867916C/en active Active
- 2012-03-22 SI SI201230926A patent/SI2828399T1/sl unknown
- 2012-03-22 WO PCT/GB2012/050645 patent/WO2013140107A1/en not_active Ceased
- 2012-03-22 LT LTEP12710994.0T patent/LT2828399T/lt unknown
- 2012-03-22 PL PL12710994T patent/PL2828399T3/pl unknown
- 2012-03-22 JP JP2015500972A patent/JP6166342B2/ja active Active
- 2012-03-22 SG SG11201502154UA patent/SG11201502154UA/en unknown
- 2012-03-22 HU HUE12710994A patent/HUE032212T2/en unknown
-
2017
- 2017-04-13 CY CY20171100441T patent/CY1118903T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| SG11201502154UA (en) | 2015-05-28 |
| NZ630915A (en) | 2016-08-26 |
| AU2012374566A1 (en) | 2014-10-02 |
| PT2828399T (pt) | 2017-04-04 |
| SI2828399T1 (sl) | 2017-05-31 |
| DK2828399T3 (en) | 2017-04-10 |
| EP2828399A1 (en) | 2015-01-28 |
| RS55868B1 (sr) | 2017-08-31 |
| ES2620750T3 (es) | 2017-06-29 |
| WO2013140107A1 (en) | 2013-09-26 |
| AU2012374566A2 (en) | 2015-12-24 |
| HRP20170569T1 (hr) | 2017-06-30 |
| CA2867916A1 (en) | 2013-09-26 |
| JP2015512251A (ja) | 2015-04-27 |
| CN104379763A (zh) | 2015-02-25 |
| PL2828399T3 (pl) | 2017-07-31 |
| LT2828399T (lt) | 2017-04-10 |
| EP2828399B1 (en) | 2017-01-18 |
| HUE032212T2 (en) | 2017-09-28 |
| AU2012374566B2 (en) | 2018-05-10 |
| CY1118903T1 (el) | 2018-01-10 |
| JP6166342B2 (ja) | 2017-07-19 |
| CN104379763B (zh) | 2017-04-19 |
| HK1204015A1 (en) | 2015-11-06 |
| CA2867916C (en) | 2020-05-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IN2014DN07878A (enrdf_load_stackoverflow) | ||
| JP5851496B2 (ja) | 修飾ステムループオリゴヌクレオチドが仲介する逆転写および塩基間隔が制限された定量的ｐｃｒ | |
| WO2014012107A3 (en) | Human identifiation using a panel of snps | |
| NZ629701A (en) | Target detection and signal amplification | |
| WO2013003630A3 (en) | Methods and compositions for enrichment of nucleic acids in mixtures of highly homologous sequences | |
| WO2011106724A3 (en) | Fast pcr for str genotyping | |
| NZ723399A (en) | Methods and compositions for dna profiling | |
| MX2018013367A (es) | Metodos para etiquetar bibliotecas codificadas por adn. | |
| EP4310196A3 (en) | Methods and compositions for manipulating nucleic acids | |
| NZ723570A (en) | Compositions and methods for quantifying a nucleic acid sequence in a sample | |
| SG194722A1 (en) | Probe based nucleic acid detection | |
| JP2011502502A5 (enrdf_load_stackoverflow) | ||
| EA202191796A2 (ru) | Способ сайт-специфического ферментативного мечения нуклеиновых кислот in vitro введением не встречающихся в природе нуклеотидов | |
| IN2013MN00522A (enrdf_load_stackoverflow) | ||
| JP2012520080A5 (enrdf_load_stackoverflow) | ||
| WO2012135053A3 (en) | Rnase h-based assays utilizing modified rna monomers | |
| WO2007127999A3 (en) | Use of products of pcr amplification carrying elements of secondary structure to improve pcr-based nucleic acid detection | |
| WO2012067830A3 (en) | Methylation assay | |
| AU2009355009A8 (en) | THD primer target detection | |
| JP2012514451A5 (enrdf_load_stackoverflow) | ||
| BR112013033462A2 (pt) | método para a redução de complexidade do genoma e detecção de polimorfismo | |
| WO2012177695A3 (en) | Hybrid polymerases having the ability to produce long amplicons | |
| NZ772437A (en) | Multiplex detection of nucleic acids | |
| WO2014118377A3 (en) | Primer technology | |
| ATE497022T1 (de) | Verfahren zur amplifikation von nukleinsäure |