IN2012DN03823A - - Google Patents

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Publication number
IN2012DN03823A
IN2012DN03823A IN3823DEN2012A IN2012DN03823A IN 2012DN03823 A IN2012DN03823 A IN 2012DN03823A IN 3823DEN2012 A IN3823DEN2012 A IN 3823DEN2012A IN 2012DN03823 A IN2012DN03823 A IN 2012DN03823A
Authority
IN
India
Prior art keywords
modified
vacuolar sorting
vps10
sorting activity
host cell
Prior art date
Application number
Other languages
English (en)
Inventor
Michael Meehl
Heping Lin
Byung-Kwon Choi
Original Assignee
Merck Sharp & Dohme
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Sharp & Dohme filed Critical Merck Sharp & Dohme
Publication of IN2012DN03823A publication Critical patent/IN2012DN03823A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
IN3823DEN2012 2009-10-30 2010-10-25 IN2012DN03823A (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25637909P 2009-10-30 2009-10-30
US35066810P 2010-06-02 2010-06-02
PCT/US2010/053903 WO2011053541A1 (fr) 2009-10-30 2010-10-25 Procédés de production de protéines recombinantes présentant de meilleurs rendements de sécrétions

Publications (1)

Publication Number Publication Date
IN2012DN03823A true IN2012DN03823A (fr) 2015-08-28

Family

ID=43922476

Family Applications (1)

Application Number Title Priority Date Filing Date
IN3823DEN2012 IN2012DN03823A (fr) 2009-10-30 2010-10-25

Country Status (12)

Country Link
US (1) US20130011875A1 (fr)
EP (1) EP2494053A4 (fr)
JP (1) JP2013509180A (fr)
KR (1) KR20140015137A (fr)
CN (1) CN102686731A (fr)
AU (1) AU2010313608A1 (fr)
BR (1) BR112012009886A2 (fr)
CA (1) CA2777487A1 (fr)
IN (1) IN2012DN03823A (fr)
MX (1) MX2012004993A (fr)
RU (1) RU2012122166A (fr)
WO (1) WO2011053541A1 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2494050A4 (fr) * 2009-10-30 2013-10-30 Merck Sharp & Dohme Facteur de stimulation des granulocytes et monocytes produit dans pichia pastoris glycomodifié
JP5932766B2 (ja) * 2011-03-11 2016-06-08 株式会社カネカ Ｖｐｓ遺伝子が破壊されている酵母を用いる異種タンパク質の製造方法
AU2013207165B2 (en) 2012-01-05 2018-10-04 Glykos Finland Oy Protease deficient filamentous fungal cells and methods of use thereof
CN102559740B (zh) * 2012-02-25 2013-03-27 山东大学 一种提高酿酒酵母分泌表达异源蛋白的方法及专用酿酒酵母菌株
WO2013174927A1 (fr) 2012-05-23 2013-11-28 Novartis International Pharmaceutical Limited Production de glycoprotéines fucosylées
AU2014224171B2 (en) * 2013-03-06 2018-02-01 Protalix Ltd. Tnf alpha inhibitor polypeptides, polynucleotides encoding same, cells expressing same and methods of producing same
CN105980552A (zh) 2013-07-10 2016-09-28 诺华股份有限公司 多种蛋白酶缺陷的丝状真菌细胞及其使用方法
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
BR112017010555A2 (pt) * 2014-11-20 2018-02-27 Yissum Res Dev Co Of Hebrew Univ Jerusalem Ltd composições e métodos para produção de polipeptídeos com padrão de glicosilação modificado em células vegetais
US10988515B2 (en) 2017-01-13 2021-04-27 Bolt Threads, Inc. Elastomeric proteins
US11795487B2 (en) * 2017-07-31 2023-10-24 Melt & Marble AB Fungal cell with improved protein production capacity
SE2051317A1 (en) * 2020-11-11 2022-05-12 Nielsen Dina Petranovic A genetically modified yeast cell
CN113604373A (zh) * 2021-02-08 2021-11-05 江南大学 一种提高人源溶菌酶产量及酶活的毕赤酵母缺陷型菌株
CN113736818A (zh) * 2021-10-08 2021-12-03 江南大学 一种毕赤酵母中提高人源溶菌酶分泌效率及酶活的方法
EP4194560A1 (fr) 2021-12-08 2023-06-14 European Molecular Biology Laboratory Production améliorée de protéines sécrétées dans des cellules fongiques

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4818700A (en) 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
AU2001256762A1 (en) 2000-05-17 2001-11-26 Mitsubishi Pharma Corporation Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US8697394B2 (en) 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
PT1522590E (pt) 2000-06-28 2009-10-26 Glycofi Inc Métodos para a produção de glicoproteínas modificadas
US7598055B2 (en) 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7795002B2 (en) 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
GB0121088D0 (en) 2001-08-31 2001-10-24 Cambridge Consultants Electric kettle
EP2359685B1 (fr) 2001-12-27 2013-12-04 GlycoFi, Inc. Procede d'ingenierie de structures de sucres typiques des mammiferes
IL165717A0 (en) * 2002-06-26 2006-01-15 Flanders Interuniversity Inst A strain of methylotrophic yeast for producing proteins
US7705203B2 (en) 2002-11-12 2010-04-27 Purdue Research Foundation Benzoate inductible promoters
ES2528739T3 (es) 2003-12-24 2015-02-12 Glycofi, Inc. Métodos para eliminar la manosilfosforilación de glucanos en la producción de glucoproteínas
US7479389B2 (en) 2004-03-02 2009-01-20 Glycofi, Inc. ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and methods for stable genetic integration
JP4932699B2 (ja) 2004-03-17 2012-05-16 グライコフィ， インコーポレイテッド 真菌および酵母におけるシチジンモノホスフェート−シアル酸合成経路を操作する方法
CA2565125A1 (fr) 2004-04-29 2005-11-10 Glycofi, Inc. Methodes servant a diminuer ou a eliminer des glycanes resistant a alpha-mannosidase dans la production de glycoproteines
CA2606253A1 (fr) 2005-04-21 2006-10-26 Uti Limited Partnership Reaction en chaine de la polymerase (pcr) pour typage sccmec mrsa
WO2007136752A2 (fr) * 2006-05-19 2007-11-29 Glycofi, Inc. Compositions d'érythropoïétine
CN101535340B (zh) 2006-05-19 2013-05-01 格利科菲公司 促红细胞生成素组合物

Also Published As

Publication number Publication date
BR112012009886A2 (pt) 2015-09-15
WO2011053541A1 (fr) 2011-05-05
RU2012122166A (ru) 2013-12-10
US20130011875A1 (en) 2013-01-10
CN102686731A (zh) 2012-09-19
MX2012004993A (es) 2012-06-12
EP2494053A4 (fr) 2013-10-30
KR20140015137A (ko) 2014-02-06
EP2494053A1 (fr) 2012-09-05
AU2010313608A1 (en) 2012-06-07
JP2013509180A (ja) 2013-03-14
CA2777487A1 (fr) 2011-05-05

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