MX353498B - Elementos de adn baculoviral para la expresion de proteinas recombinantes en una celula huesped. - Google Patents
Elementos de adn baculoviral para la expresion de proteinas recombinantes en una celula huesped.Info
- Publication number
- MX353498B MX353498B MX2014015277A MX2014015277A MX353498B MX 353498 B MX353498 B MX 353498B MX 2014015277 A MX2014015277 A MX 2014015277A MX 2014015277 A MX2014015277 A MX 2014015277A MX 353498 B MX353498 B MX 353498B
- Authority
- MX
- Mexico
- Prior art keywords
- host cell
- dna elements
- expression
- recombinant
- elements
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 3
- 108020004511 Recombinant DNA Proteins 0.000 abstract 3
- 108700028146 Genetic Enhancer Elements Proteins 0.000 abstract 1
- 230000004663 cell proliferation Effects 0.000 abstract 1
- 230000003833 cell viability Effects 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000002103 transcriptional effect Effects 0.000 abstract 1
- 108091008023 transcriptional regulators Proteins 0.000 abstract 1
- 241000701447 unidentified baculovirus Species 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
- A01K67/0337—Genetically modified Arthropods
- A01K67/0339—Genetically modified insects, e.g. Drosophila melanogaster, medfly
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- C12N15/866—Baculoviral vectors
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/70—Invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/70—Invertebrates
- A01K2227/706—Insects, e.g. Drosophila melanogaster, medfly
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/01—Animal expressing industrially exogenous proteins
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/02—Animal zootechnically ameliorated
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
- C12N2710/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
- C12N2710/14141—Use of virus, viral particle or viral elements as a vector
- C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/60—Vector systems having a special element relevant for transcription from viruses
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Environmental Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Animal Behavior & Ethology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
La presente invención se refiere a un baculovirus recombinante que contiene un gen endógeno Ac-ie-01, que comprende [i] una copia adicional de ADNc de Ac-ie-01 baculoviral como transgen bajo el control de un promotor adecuado que permite la expresión por encima de niveles endógenos de las proteínas IE-1, IE-0 y/o fragmentos de las mismas que funcionan como reguladores de la transcripción, en donde el ácido nucleico se selecciona a partir del grupo que consiste de: a) un ácido nucleico que contiene la secuencia de nucleótido indicada en cualquiera de la SEQ ID NO: 1-5, b) una secuencia de ácido nucleico que tiene una identidad de secuencia de por lo menos 70%, de preferencia de por lo menos 75%, más preferido de por lo menos 80%, más preferido de por lo menos 85%, más preferido de por lo menos 90% y más preferido de por lo menos 95% con la secuencia de nucleótido indica en cualquiera de SEQ ID NO: 1-5 y que codifica para una proteína que puede funcionar como un regulador de la transcripción en un baculovirus recombinante; c) una secuencia de ácido nucleico que codifica para un aminoácido que contiene la secuencia de aminoácido indicada en cualquiera de SEQ ID NO: 6-9; y d) una secuencia de ácido nucleico que codifica para una secuencia de aminoácido que tiene una similitud de secuencia de por lo menos 70%, de preferencia de por lo menos 75%, más preferido de por lo menos 80%, más preferido de por lo menos 85%, más preferido de por lo menos 90% y más preferido de por lo menos 95% con la secuencia de aminoácido indicada en cualquiera de SEQ ID NO: 6-9 y que puede funcionar como un regulador de la transcripción en un baculovirus recombinante; [ii] en donde el baculovirus recombinante comprende también por lo menos una región recombinante homóloga (hr) como región potenciadora, ligada de la manera operable a cualquier promotor que es adecuado para impulsar la expresión de una proteína recombinante. [iii] en donde el promotor ligado de manera operable a la región homóloga (hr) se selecciona a partir del grupo de ácidos nucleicos que comprende: a) un ácido nucleico que contiene la secuencia de nucleótido indicada en cualquiera de SEQ ID NO: 10-16; y b) una secuencia de ácido nucleico que puede funcionar como un promotor en un baculovirus recombinante y que tiene una identidad de secuencia de por lo menos 70%, de preferencia de por lo menos 75%, más preferido de por lo menos 80%, más preferido de por lo menos 85%, más preferido de por lo menos 90% y más preferido aún de por lo menos 95% con la secuencia de nucleótido indicada en cualquiera de SEQ ID NO: 10-16.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11169634 | 2011-06-10 | ||
| EP2011068381 | 2011-10-20 | ||
| EP2011072406 | 2011-12-12 | ||
| PCT/EP2012/061081 WO2012168492A2 (en) | 2011-06-10 | 2012-06-12 | Recombinant dna elements for the expression of recombinant proteins in a host cell |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2014015277A MX2014015277A (es) | 2015-12-15 |
| MX353498B true MX353498B (es) | 2018-01-16 |
Family
ID=47296540
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014015277A MX353498B (es) | 2011-06-10 | 2012-06-12 | Elementos de adn baculoviral para la expresion de proteinas recombinantes en una celula huesped. |
Country Status (9)
| Country | Link |
|---|---|
| EP (2) | EP2858490B1 (es) |
| BR (1) | BR112014031039A2 (es) |
| CA (2) | CA2876343A1 (es) |
| DK (2) | DK2858489T3 (es) |
| IN (1) | IN2014MN02500A (es) |
| MX (1) | MX353498B (es) |
| PL (2) | PL2858489T3 (es) |
| PT (2) | PT2858490T (es) |
| WO (6) | WO2012168788A2 (es) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9982239B2 (en) | 2012-06-12 | 2018-05-29 | Alternative Gene Expression S.L. | Baculoviral DNA elements for the expression of recombinant proteins in a host cell |
| ES2612854T3 (es) * | 2012-06-12 | 2017-05-19 | Alternative Gene Expression, S.L. | Elementos de ADN recombinante para la expresión de proteínas recombinantes en una célula huésped |
| CN105392362B (zh) | 2012-06-12 | 2018-11-09 | 替代基因表达公司 | 用于在宿主昆虫中表达重组蛋白的杆状病毒dna元件 |
| CN103749393A (zh) * | 2013-12-20 | 2014-04-30 | 铜陵桂生生态养殖有限公司 | 一种降低蝎子死亡率的养殖方法 |
| ES2554561B1 (es) * | 2014-06-16 | 2016-09-30 | Universitat De València | Nuevos promotores derivados de baculovirus con elevada actividad en sistemas de expresión basados en baculovirus |
| CN108474004A (zh) | 2015-09-17 | 2018-08-31 | 替代基因公司 | 重组蛋白在粉纹夜蛾蛹中的表达 |
| CN105087606A (zh) * | 2015-09-23 | 2015-11-25 | 天津瑞普生物技术股份有限公司 | 猪圆环病毒2型cap抗原制备方法 |
| CN105238816B (zh) * | 2015-10-26 | 2018-08-24 | 广州汉腾生物科技有限公司 | 一种表达载体 |
| WO2018176075A1 (en) * | 2017-03-27 | 2018-10-04 | The University Of Queensland | Chimeric insect-specific flaviviruses |
| HUE059211T2 (hu) | 2018-06-20 | 2022-10-28 | Fatro Spa | Vakcinák vérzéses nyúlbetegség megelõzésére |
| CN110964749A (zh) * | 2018-09-29 | 2020-04-07 | 普莱柯生物工程股份有限公司 | 一种在杆状病毒表达系统中高效表达外源蛋白的方法及其应用 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995017515A1 (en) * | 1993-12-23 | 1995-06-29 | University Technologies International Inc. | Methods of expressing proteins in insect cells and methods of killing insects |
| US5965393A (en) * | 1997-07-01 | 1999-10-12 | National Institute Of Immunology | Method for enhancing foreign gene expression in baculovirus expression vector system |
| RU2158505C2 (ru) * | 1998-06-09 | 2000-11-10 | Совхоз "Тепличный" | Способ приготовления углеводного корма для насекомых-опылителей |
| ES2232308B1 (es) | 2003-11-12 | 2006-10-16 | Laboratorios Calier, S.A. | Dispositivo para la cria de larvas. |
| JP4271122B2 (ja) * | 2004-10-15 | 2009-06-03 | 財団法人 ひろしま産業振興機構 | カイコでの組換えタンパク質製造のためのポリヌクレオチド |
| US8148129B2 (en) * | 2006-06-30 | 2012-04-03 | The Regents Of The University Of California | Generation of potent dominant negative transcriptional inhibitors |
| US8105827B2 (en) * | 2007-09-06 | 2012-01-31 | Academia Sinica | Protein expression systems |
| BRPI0823074A2 (pt) * | 2008-09-02 | 2012-04-10 | Alternative Gene Expression S L Algenex | método para desenvolver sequências regulatórias polinucleotìdicas, sequências regulatórias polinucleotìdicas, uso das sequências regulatórias polinucleotìdicas, vetor de expressão, células transformadas ou transfectadas, larvas de inseto transfectadas, método para produzir proteìnas recombinantes, uso do vetor de expressão e uso das larvas de inseto |
-
2012
- 2012-06-12 DK DK12750333.2T patent/DK2858489T3/da active
- 2012-06-12 MX MX2014015277A patent/MX353498B/es active IP Right Grant
- 2012-06-12 PL PL12750333T patent/PL2858489T3/pl unknown
- 2012-06-12 CA CA2876343A patent/CA2876343A1/en not_active Abandoned
- 2012-06-12 WO PCT/IB2012/001136 patent/WO2012168788A2/en active Application Filing
- 2012-06-12 IN IN2500MUN2014 patent/IN2014MN02500A/en unknown
- 2012-06-12 EP EP12753914.6A patent/EP2858490B1/en active Active
- 2012-06-12 WO PCT/EP2012/061088 patent/WO2012168493A2/en active Application Filing
- 2012-06-12 PT PT127539146T patent/PT2858490T/pt unknown
- 2012-06-12 CA CA2876641A patent/CA2876641C/en active Active
- 2012-06-12 BR BR112014031039A patent/BR112014031039A2/pt not_active Application Discontinuation
- 2012-06-12 EP EP12750333.2A patent/EP2858489B1/en active Active
- 2012-06-12 WO PCT/IB2012/001137 patent/WO2012168789A2/en active Application Filing
- 2012-06-12 DK DK12753914.6T patent/DK2858490T5/en active
- 2012-06-12 PT PT127503332T patent/PT2858489T/pt unknown
- 2012-06-12 PL PL12753914T patent/PL2858490T3/pl unknown
- 2012-06-12 WO PCT/EP2012/061081 patent/WO2012168492A2/en active Application Filing
- 2012-06-13 WO PCT/RU2012/000455 patent/WO2012169939A2/en active Application Filing
- 2012-06-13 WO PCT/RU2012/000456 patent/WO2012169940A2/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| CA2876343A1 (en) | 2012-12-13 |
| WO2012169940A9 (en) | 2013-02-14 |
| WO2012169939A9 (en) | 2013-02-14 |
| WO2012168789A3 (en) | 2013-05-10 |
| WO2012168788A2 (en) | 2012-12-13 |
| EP2858489A2 (en) | 2015-04-15 |
| DK2858490T5 (en) | 2017-01-23 |
| DK2858490T3 (en) | 2016-12-12 |
| PT2858489T (pt) | 2016-12-14 |
| WO2012169940A2 (en) | 2012-12-13 |
| WO2012169940A3 (en) | 2013-04-04 |
| PL2858490T3 (pl) | 2017-08-31 |
| WO2012168492A2 (en) | 2012-12-13 |
| WO2012168789A2 (en) | 2012-12-13 |
| CA2876641A1 (en) | 2012-12-13 |
| EP2858490A2 (en) | 2015-04-15 |
| WO2012168493A8 (en) | 2013-04-04 |
| DK2858489T3 (da) | 2017-01-02 |
| CA2876641C (en) | 2018-09-25 |
| WO2012168492A3 (en) | 2013-04-11 |
| BR112014031039A2 (pt) | 2018-05-08 |
| EP2858489B1 (en) | 2016-11-09 |
| MX2014015277A (es) | 2015-12-15 |
| WO2012168493A2 (en) | 2012-12-13 |
| EP2858490B1 (en) | 2016-10-26 |
| PL2858489T3 (pl) | 2017-07-31 |
| PT2858490T (pt) | 2016-11-28 |
| WO2012168493A3 (en) | 2013-05-23 |
| WO2012168788A3 (en) | 2013-05-10 |
| WO2012169939A3 (en) | 2013-05-10 |
| IN2014MN02500A (es) | 2015-07-17 |
| WO2012169939A2 (en) | 2012-12-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MX2014015277A (es) | Elementos de adn baculoviral para la expresion de proteinas recombinantes en una celula huesped. | |
| MX2019002498A (es) | Metodos y productos para la expresion de proteinas en celulas. | |
| MX357290B (es) | Mejoramiento de la actividad de proteínas que requieren centros fe-s. | |
| EP4372078A3 (en) | Methods for harvesting mammalian cell cultures | |
| MX2021000211A (es) | Metodos y productos para transfeccion de celulas. | |
| WO2012074277A3 (en) | Novel hybrid promoter and recombinant vector comprising the same | |
| EP3839055A3 (en) | Yeast promoters for protein expression | |
| BR112017008827A2 (pt) | célula recombinante cho, cultura em larga escala, e, composição. | |
| MX2013003236A (es) | Variantes de polipeptidos que tienen actividad potenciadora celulolitica y polinucleotidos que codifican a los mismos. | |
| MX2013003237A (es) | Variantes de polipeptidos que tienen actividad potenciadora celulolitica y polinucleotidos que codifican a los mismos. | |
| BR112015003724A2 (pt) | polipeptídeo isolado, composição, uso de uma composição, polinucleotídeo isolado, constructo de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, e, métodos de produção de um polipeptídeo e de produção de uma proteína. | |
| MX358963B (es) | Polipeptidos con actividad proteasa. | |
| EP3180463A4 (en) | Detecting residual host cell proteins in recombinant protein preparations | |
| MX356162B (es) | Sistema de expresion y secrecion. | |
| MY187130A (en) | Variants of gal2 transporter and their uses | |
| PL3736329T3 (pl) | Zużywalne krioprezerwowane komórki wykazujące przejściową nadekspresję genu(-ów) kodującego(-ych) białko(-a) transportera leku i/lub enzym(y) metabolizujący(-e) lek | |
| MX2017001824A (es) | Mutaciones en proteinas del grupo hierro-azufre que mejoran la utilizacion de xilosa. | |
| MD20130099A2 (ro) | Expresia recombinantă a interferonului solubil | |
| BR112013014869A2 (pt) | método para produzir um polipeptídeo, promotor isolado, construção de ácido nucleico, e, célula hospedeira recombinante | |
| NZ717178A (en) | Expression constructs and methods for expressing polypeptides in eukaryotic cells | |
| ES2523016A1 (es) | Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes | |
| WO2012109220A3 (en) | Cell cycle control for improving process performance and recombinant expression in fungal host cells | |
| WO2016022514A3 (en) | Inhibitory rna for enhanced protein production in recombinant mammalian cells | |
| IN2012DE03699A (es) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |