PT2858490T - Elementos de adn recombinantes para a expressão de proteínas recombinantes em insetos - Google Patents

Elementos de adn recombinantes para a expressão de proteínas recombinantes em insetos

Info

Publication number
PT2858490T
PT2858490T PT127539146T PT12753914T PT2858490T PT 2858490 T PT2858490 T PT 2858490T PT 127539146 T PT127539146 T PT 127539146T PT 12753914 T PT12753914 T PT 12753914T PT 2858490 T PT2858490 T PT 2858490T
Authority
PT
Portugal
Prior art keywords
recombinant
insects
expression
dna elements
recombinant dna
Prior art date
Application number
PT127539146T
Other languages
English (en)
Inventor
Gomez Sebastian Silvia
López Vidal Javier
Angel Martinez Escribano José
Original Assignee
Alternative Gene Expression Sl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alternative Gene Expression Sl filed Critical Alternative Gene Expression Sl
Publication of PT2858490T publication Critical patent/PT2858490T/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
    • A01K67/0337—Genetically modified Arthropods
    • A01K67/0339—Genetically modified insects, e.g. Drosophila melanogaster, medfly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/866—Baculoviral vectors
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A01K2227/706—Insects, e.g. Drosophila melanogaster, medfly
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses
PT127539146T 2011-06-10 2012-06-12 Elementos de adn recombinantes para a expressão de proteínas recombinantes em insetos PT2858490T (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11169634 2011-06-10
EP2011068381 2011-10-20
EP2011072406 2011-12-12

Publications (1)

Publication Number Publication Date
PT2858490T true PT2858490T (pt) 2016-11-28

Family

ID=47296540

Family Applications (2)

Application Number Title Priority Date Filing Date
PT127539146T PT2858490T (pt) 2011-06-10 2012-06-12 Elementos de adn recombinantes para a expressão de proteínas recombinantes em insetos
PT127503332T PT2858489T (pt) 2011-06-10 2012-06-12 Elementos de adn recombinante para a expressão de proteínas recombinantes numa célula hospedeira

Family Applications After (1)

Application Number Title Priority Date Filing Date
PT127503332T PT2858489T (pt) 2011-06-10 2012-06-12 Elementos de adn recombinante para a expressão de proteínas recombinantes numa célula hospedeira

Country Status (9)

Country Link
EP (2) EP2858490B1 (pt)
BR (1) BR112014031039A2 (pt)
CA (2) CA2876343A1 (pt)
DK (2) DK2858490T5 (pt)
IN (1) IN2014MN02500A (pt)
MX (1) MX353498B (pt)
PL (2) PL2858489T3 (pt)
PT (2) PT2858490T (pt)
WO (6) WO2012168492A2 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104735977B (zh) 2012-06-12 2016-09-21 替代基因表达公司 用于在宿主细胞中表达重组蛋白的杆状病毒dna元件
JP6143853B2 (ja) 2012-06-12 2017-06-07 オルタナティブ ジーン エクスプレッション エセ．エレ． 宿主細胞における組換えタンパク質の発現のためのバキュロウイルスｄｎａエレメント
JP6063563B2 (ja) 2012-06-12 2017-01-18 オルタナティブ ジーン エクスプレッション エセ．エレ． 宿主昆虫における組換えタンパク質の発現のためのバキュロウイルスｄｎａエレメント
CN103749393A (zh) * 2013-12-20 2014-04-30 铜陵桂生生态养殖有限公司 一种降低蝎子死亡率的养殖方法
ES2554561B1 (es) * 2014-06-16 2016-09-30 Universitat De València Nuevos promotores derivados de baculovirus con elevada actividad en sistemas de expresión basados en baculovirus
EP3350334B8 (en) 2015-09-17 2020-02-19 Alternative Gene Expression, S.L. Expression of recombinant proteins in trichoplusia ni pupae
CN105087606A (zh) * 2015-09-23 2015-11-25 天津瑞普生物技术股份有限公司 猪圆环病毒2型cap抗原制备方法
CN105238816B (zh) * 2015-10-26 2018-08-24 广州汉腾生物科技有限公司 一种表达载体
US11572390B2 (en) 2017-03-27 2023-02-07 The University Of Queensland Chimeric insect-specific flaviviruses
HUE059211T2 (hu) 2018-06-20 2022-10-28 Fatro Spa Vakcinák vérzéses nyúlbetegség megelõzésére
CN110964749A (zh) * 2018-09-29 2020-04-07 普莱柯生物工程股份有限公司 一种在杆状病毒表达系统中高效表达外源蛋白的方法及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1377395A (en) * 1993-12-23 1995-07-10 University Technologies International Inc. Methods of expressing proteins in insect cells and methods of killing insects
US5965393A (en) * 1997-07-01 1999-10-12 National Institute Of Immunology Method for enhancing foreign gene expression in baculovirus expression vector system
RU2158505C2 (ru) * 1998-06-09 2000-11-10 Совхоз "Тепличный" Способ приготовления углеводного корма для насекомых-опылителей
ES2232308B1 (es) 2003-11-12 2006-10-16 Laboratorios Calier, S.A. Dispositivo para la cria de larvas.
JP4271122B2 (ja) * 2004-10-15 2009-06-03 財団法人 ひろしま産業振興機構 カイコでの組換えタンパク質製造のためのポリヌクレオチド
US8148129B2 (en) * 2006-06-30 2012-04-03 The Regents Of The University Of California Generation of potent dominant negative transcriptional inhibitors
US8105827B2 (en) * 2007-09-06 2012-01-31 Academia Sinica Protein expression systems
CN102203264A (zh) * 2008-09-02 2011-09-28 替代基因表达有限公司(艾尔基尼克斯) 用于在昆虫细胞中外源蛋白质表达的昆虫衍生启动子

Also Published As

Publication number Publication date
IN2014MN02500A (pt) 2015-07-17
WO2012168789A3 (en) 2013-05-10
PT2858489T (pt) 2016-12-14
EP2858490A2 (en) 2015-04-15
WO2012169939A2 (en) 2012-12-13
MX2014015277A (es) 2015-12-15
WO2012168492A2 (en) 2012-12-13
WO2012168788A3 (en) 2013-05-10
WO2012168789A2 (en) 2012-12-13
PL2858490T3 (pl) 2017-08-31
DK2858489T3 (da) 2017-01-02
WO2012169939A9 (en) 2013-02-14
DK2858490T3 (en) 2016-12-12
WO2012168493A3 (en) 2013-05-23
WO2012169939A3 (en) 2013-05-10
EP2858489B1 (en) 2016-11-09
CA2876641A1 (en) 2012-12-13
PL2858489T3 (pl) 2017-07-31
EP2858489A2 (en) 2015-04-15
BR112014031039A2 (pt) 2018-05-08
MX353498B (es) 2018-01-16
EP2858490B1 (en) 2016-10-26
CA2876641C (en) 2018-09-25
CA2876343A1 (en) 2012-12-13
WO2012168493A8 (en) 2013-04-04
WO2012168492A3 (en) 2013-04-11
DK2858490T5 (en) 2017-01-23
WO2012168788A2 (en) 2012-12-13
WO2012169940A9 (en) 2013-02-14
WO2012169940A2 (en) 2012-12-13
WO2012169940A3 (en) 2013-04-04
WO2012168493A2 (en) 2012-12-13

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