IL32910A - Material and process for determining lipase - Google Patents
Material and process for determining lipaseInfo
- Publication number
- IL32910A IL32910A IL32910A IL3291069A IL32910A IL 32910 A IL32910 A IL 32910A IL 32910 A IL32910 A IL 32910A IL 3291069 A IL3291069 A IL 3291069A IL 32910 A IL32910 A IL 32910A
- Authority
- IL
- Israel
- Prior art keywords
- agent according
- present
- emulsion
- colour
- agent
- Prior art date
Links
- 239000003795 chemical substances by application Substances 0.000 title claims description 84
- 239000004367 Lipase Substances 0.000 title claims description 53
- 108090001060 Lipase Proteins 0.000 title claims description 53
- 102000004882 Lipase Human genes 0.000 title claims description 53
- 235000019421 lipase Nutrition 0.000 title claims description 53
- 238000000034 method Methods 0.000 title claims description 26
- 230000008569 process Effects 0.000 title claims description 22
- 239000000839 emulsion Substances 0.000 claims description 73
- 239000000654 additive Substances 0.000 claims description 21
- 150000001875 compounds Chemical class 0.000 claims description 19
- 230000000996 additive effect Effects 0.000 claims description 17
- OLQIKGSZDTXODA-UHFFFAOYSA-N 4-[3-(4-hydroxy-2-methylphenyl)-1,1-dioxo-2,1$l^{6}-benzoxathiol-3-yl]-3-methylphenol Chemical group CC1=CC(O)=CC=C1C1(C=2C(=CC(O)=CC=2)C)C2=CC=CC=C2S(=O)(=O)O1 OLQIKGSZDTXODA-UHFFFAOYSA-N 0.000 claims description 15
- 230000008859 change Effects 0.000 claims description 15
- 239000000126 substance Substances 0.000 claims description 13
- -1 sulphuric acid ester Chemical class 0.000 claims description 13
- 239000011248 coating agent Substances 0.000 claims description 11
- 238000000576 coating method Methods 0.000 claims description 11
- 238000011534 incubation Methods 0.000 claims description 11
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- 230000009471 action Effects 0.000 claims description 9
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- 230000001681 protective effect Effects 0.000 claims description 8
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 claims description 6
- 239000000194 fatty acid Substances 0.000 claims description 6
- VKYKSIONXSXAKP-UHFFFAOYSA-N hexamethylenetetramine Chemical compound C1N(C2)CN3CN1CN2C3 VKYKSIONXSXAKP-UHFFFAOYSA-N 0.000 claims description 6
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- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 5
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- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical compound C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 claims description 2
- 239000004593 Epoxy Substances 0.000 claims description 2
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- 239000003349 gelling agent Substances 0.000 claims description 2
- JDNTWHVOXJZDSN-UHFFFAOYSA-N iodoacetic acid Chemical compound OC(=O)CI JDNTWHVOXJZDSN-UHFFFAOYSA-N 0.000 claims description 2
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- 229920001059 synthetic polymer Polymers 0.000 claims description 2
- 229920000642 polymer Polymers 0.000 claims 2
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical class C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims 1
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- 229940100892 mercury compound Drugs 0.000 claims 1
- 239000001117 sulphuric acid Substances 0.000 claims 1
- 235000011149 sulphuric acid Nutrition 0.000 claims 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 claims 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 45
- 239000000243 solution Substances 0.000 description 43
- 229940040461 lipase Drugs 0.000 description 42
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 13
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- PNEYBMLMFCGWSK-UHFFFAOYSA-N Alumina Chemical compound [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 6
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- QAQJMLQRFWZOBN-LAUBAEHRSA-N L-ascorbyl-6-palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](O)[C@H]1OC(=O)C(O)=C1O QAQJMLQRFWZOBN-LAUBAEHRSA-N 0.000 description 3
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- 238000006073 displacement reaction Methods 0.000 description 3
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 3
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- AGDSCTQQXMDDCV-UHFFFAOYSA-M sodium;2-iodoacetate Chemical compound [Na+].[O-]C(=O)CI AGDSCTQQXMDDCV-UHFFFAOYSA-M 0.000 description 3
- 238000004448 titration Methods 0.000 description 3
- ZAKOWWREFLAJOT-UHFFFAOYSA-N DL-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-UHFFFAOYSA-N 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
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- NEHMKBQYUWJMIP-UHFFFAOYSA-N chloromethane Chemical compound ClC NEHMKBQYUWJMIP-UHFFFAOYSA-N 0.000 description 2
- 239000002812 cholic acid derivative Substances 0.000 description 2
- JHIVVAPYMSGYDF-UHFFFAOYSA-N cyclohexanone Chemical compound O=C1CCCCC1 JHIVVAPYMSGYDF-UHFFFAOYSA-N 0.000 description 2
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- ZTFYJIXFKGPCHV-UHFFFAOYSA-N 2-propan-2-ylnaphthalene-1-sulfonic acid Chemical class C1=CC=CC2=C(S(O)(=O)=O)C(C(C)C)=CC=C21 ZTFYJIXFKGPCHV-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
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- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 description 1
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- IJCWFDPJFXGQBN-RYNSOKOISA-N [(2R)-2-[(2R,3R,4S)-4-hydroxy-3-octadecanoyloxyoxolan-2-yl]-2-octadecanoyloxyethyl] octadecanoate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@@H](OC(=O)CCCCCCCCCCCCCCCCC)[C@H]1OC[C@H](O)[C@H]1OC(=O)CCCCCCCCCCCCCCCCC IJCWFDPJFXGQBN-RYNSOKOISA-N 0.000 description 1
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- 159000000000 sodium salts Chemical class 0.000 description 1
- JAJWGJBVLPIOOH-IZYKLYLVSA-M sodium taurocholate Chemical compound [Na+].C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 JAJWGJBVLPIOOH-IZYKLYLVSA-M 0.000 description 1
- VMOXILJFUFEZJL-UHFFFAOYSA-M sodium;ethylmercury;2-sulfanylbenzoate Chemical compound [Na+].CC[Hg].[O-]C(=O)C1=CC=CC=C1S VMOXILJFUFEZJL-UHFFFAOYSA-M 0.000 description 1
- 239000001593 sorbitan monooleate Substances 0.000 description 1
- 235000011069 sorbitan monooleate Nutrition 0.000 description 1
- 229940035049 sorbitan monooleate Drugs 0.000 description 1
- 239000001587 sorbitan monostearate Substances 0.000 description 1
- 235000011076 sorbitan monostearate Nutrition 0.000 description 1
- 229940035048 sorbitan monostearate Drugs 0.000 description 1
- 229950004959 sorbitan oleate Drugs 0.000 description 1
- 229950003429 sorbitan palmitate Drugs 0.000 description 1
- 229950011392 sorbitan stearate Drugs 0.000 description 1
- 235000019337 sorbitan trioleate Nutrition 0.000 description 1
- 229960000391 sorbitan trioleate Drugs 0.000 description 1
- 239000001589 sorbitan tristearate Substances 0.000 description 1
- 235000011078 sorbitan tristearate Nutrition 0.000 description 1
- 229960004129 sorbitan tristearate Drugs 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 229940042585 tocopherol acetate Drugs 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- PHYFQTYBJUILEZ-IUPFWZBJSA-N triolein Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(OC(=O)CCCCCCC\C=C/CCCCCCCC)COC(=O)CCCCCCC\C=C/CCCCCCCC PHYFQTYBJUILEZ-IUPFWZBJSA-N 0.000 description 1
- 229940117972 triolein Drugs 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19681798285 DE1798285C3 (de) | 1968-09-20 | Neues Mittel und Verfahren zur Lipasebesti m mu ng | |
| DE19691943454 DE1943454A1 (de) | 1968-09-20 | 1969-08-27 | Neues Mittel und Verfahren zur Lipascbestimmung |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL32910A0 IL32910A0 (en) | 1969-11-30 |
| IL32910A true IL32910A (en) | 1972-11-28 |
Family
ID=25756172
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL32910A IL32910A (en) | 1968-09-20 | 1969-08-28 | Material and process for determining lipase |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US3689364A (enExample) |
| JP (1) | JPS5542703B1 (enExample) |
| BE (1) | BE738960A (enExample) |
| CH (1) | CH506792A (enExample) |
| DE (1) | DE1943454A1 (enExample) |
| FR (1) | FR2022180A1 (enExample) |
| GB (1) | GB1247449A (enExample) |
| IL (1) | IL32910A (enExample) |
| NL (1) | NL6913769A (enExample) |
| SE (1) | SE372631B (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3917515A (en) * | 1974-03-13 | 1975-11-04 | Jack M Goldberg | Serum lipase method and medium |
| CA1077498A (en) * | 1975-02-28 | 1980-05-13 | George F. Bulbenko | Thymol blue monophosphates and salts thereof |
| US4045290A (en) * | 1975-02-28 | 1977-08-30 | Princeton Biomedix Incorporated | Diagnostic method and compounds for use therewith |
| US4275152A (en) * | 1977-02-03 | 1981-06-23 | Eastman Kodak Company | Hydrolysis of protein-bound cholesterol esters |
| US4275151A (en) * | 1977-02-03 | 1981-06-23 | Eastman Kodak Company | Hydrolysis of protein-bound cholesterol esters |
| US4347313A (en) * | 1978-02-27 | 1982-08-31 | Boehringer Mannheim Gmbh | Analytical determination of lipase |
| DE2826965A1 (de) * | 1978-06-20 | 1980-01-17 | Boehringer Mannheim Gmbh | Diagnostisches mittel zum nachweis von leukozyten in koerperfluessigkeiten und dafuer geeignete chromogene |
| DE2905531A1 (de) * | 1979-02-14 | 1981-01-08 | Boehringer Mannheim Gmbh | Diagnostisches mittel zum nachweis von leukozyten in koerperfluessigkeiten |
| EP0021572A1 (en) * | 1979-06-04 | 1981-01-07 | American Hospital Supply Corporation | A substrate for use in a turbidimetric assay for lipase and a method for making this substrate |
| JPS6035117B2 (ja) * | 1980-10-28 | 1985-08-13 | 富士写真フイルム株式会社 | リパ−ゼ活性測定用試薬 |
| US4555483A (en) * | 1982-08-11 | 1985-11-26 | Eastman Kodak Company | Methods, compositions and elements for the determination of lipase |
| DE3516001A1 (de) * | 1985-05-03 | 1986-11-06 | Boehringer Mannheim Gmbh, 6800 Mannheim | Lipasefarbtest |
| TW442570B (en) * | 1996-09-19 | 2001-06-23 | Roche Diagnostics Gmbh | Improved colour test for the detection of activity of lipase |
| US5972595A (en) * | 1997-12-19 | 1999-10-26 | Nen Life Science Products, Inc. | Enzyme assay using a solid phase substrate |
| CN100445731C (zh) * | 2004-11-12 | 2008-12-24 | 中国科学院兰州化学物理研究所 | 半胱氨酸的比色检测分析方法 |
-
1969
- 1969-07-25 CH CH1143969A patent/CH506792A/de not_active IP Right Cessation
- 1969-08-27 DE DE19691943454 patent/DE1943454A1/de active Pending
- 1969-08-28 IL IL32910A patent/IL32910A/en unknown
- 1969-09-08 GB GB44330/69A patent/GB1247449A/en not_active Expired
- 1969-09-10 NL NL6913769A patent/NL6913769A/xx unknown
- 1969-09-15 US US858154A patent/US3689364A/en not_active Expired - Lifetime
- 1969-09-16 SE SE6912716A patent/SE372631B/xx unknown
- 1969-09-17 BE BE738960A patent/BE738960A/xx unknown
- 1969-09-19 FR FR6931987A patent/FR2022180A1/fr not_active Withdrawn
- 1969-09-20 JP JP7439569A patent/JPS5542703B1/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| IL32910A0 (en) | 1969-11-30 |
| DE1798285A1 (de) | 1972-01-20 |
| GB1247449A (en) | 1971-09-22 |
| CH506792A (de) | 1971-04-30 |
| BE738960A (enExample) | 1970-03-17 |
| DE1943454A1 (de) | 1971-03-25 |
| US3689364A (en) | 1972-09-05 |
| SE372631B (enExample) | 1974-12-23 |
| FR2022180A1 (enExample) | 1970-07-31 |
| NL6913769A (enExample) | 1970-03-24 |
| DE1798285B2 (de) | 1976-02-12 |
| JPS5542703B1 (enExample) | 1980-11-01 |
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