GB1247449A - Agent for the determination of lipase - Google Patents
Agent for the determination of lipaseInfo
- Publication number
- GB1247449A GB1247449A GB44330/69A GB4433069A GB1247449A GB 1247449 A GB1247449 A GB 1247449A GB 44330/69 A GB44330/69 A GB 44330/69A GB 4433069 A GB4433069 A GB 4433069A GB 1247449 A GB1247449 A GB 1247449A
- Authority
- GB
- United Kingdom
- Prior art keywords
- emulsion
- fat
- lipase
- oil
- indicator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000003795 chemical substances by application Substances 0.000 title abstract 5
- 239000004367 Lipase Substances 0.000 title abstract 4
- 102000004882 Lipase Human genes 0.000 title abstract 4
- 108090001060 Lipase Proteins 0.000 title abstract 4
- 235000019421 lipase Nutrition 0.000 title abstract 4
- 239000000839 emulsion Substances 0.000 abstract 8
- -1 carotin Chemical class 0.000 abstract 5
- 210000001124 body fluid Anatomy 0.000 abstract 2
- 239000010839 body fluid Substances 0.000 abstract 2
- 239000010685 fatty oil Substances 0.000 abstract 2
- VKYKSIONXSXAKP-UHFFFAOYSA-N hexamethylenetetramine Chemical compound C1N(C2)CN3CN1CN2C3 VKYKSIONXSXAKP-UHFFFAOYSA-N 0.000 abstract 2
- 239000003381 stabilizer Substances 0.000 abstract 2
- XKZQKPRCPNGNFR-UHFFFAOYSA-N 2-(3-hydroxyphenyl)phenol Chemical compound OC1=CC=CC(C=2C(=CC=CC=2)O)=C1 XKZQKPRCPNGNFR-UHFFFAOYSA-N 0.000 abstract 1
- OLQIKGSZDTXODA-UHFFFAOYSA-N 4-[3-(4-hydroxy-2-methylphenyl)-1,1-dioxo-2,1$l^{6}-benzoxathiol-3-yl]-3-methylphenol Chemical compound CC1=CC(O)=CC=C1C1(C=2C(=CC(O)=CC=2)C)C2=CC=CC=C2S(=O)(=O)O1 OLQIKGSZDTXODA-UHFFFAOYSA-N 0.000 abstract 1
- 150000005168 4-hydroxybenzoic acids Chemical class 0.000 abstract 1
- 229920001817 Agar Polymers 0.000 abstract 1
- 244000060011 Cocos nucifera Species 0.000 abstract 1
- 235000013162 Cocos nucifera Nutrition 0.000 abstract 1
- ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 abstract 1
- 244000165918 Eucalyptus papuana Species 0.000 abstract 1
- 229920000084 Gum arabic Polymers 0.000 abstract 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 abstract 1
- QAQJMLQRFWZOBN-LAUBAEHRSA-N L-ascorbyl-6-palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](O)[C@H]1OC(=O)C(O)=C1O QAQJMLQRFWZOBN-LAUBAEHRSA-N 0.000 abstract 1
- 239000011786 L-ascorbyl-6-palmitate Substances 0.000 abstract 1
- 229920003171 Poly (ethylene oxide) Chemical class 0.000 abstract 1
- 239000002202 Polyethylene glycol Substances 0.000 abstract 1
- 235000019484 Rapeseed oil Nutrition 0.000 abstract 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical class OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 abstract 1
- 239000000205 acacia gum Substances 0.000 abstract 1
- 235000010489 acacia gum Nutrition 0.000 abstract 1
- 239000012190 activator Substances 0.000 abstract 1
- 239000008272 agar Substances 0.000 abstract 1
- 229910052783 alkali metal Inorganic materials 0.000 abstract 1
- 150000001340 alkali metals Chemical class 0.000 abstract 1
- 239000003963 antioxidant agent Substances 0.000 abstract 1
- 235000006708 antioxidants Nutrition 0.000 abstract 1
- 235000010385 ascorbyl palmitate Nutrition 0.000 abstract 1
- 150000001540 azides Chemical class 0.000 abstract 1
- 239000003613 bile acid Substances 0.000 abstract 1
- 230000002599 biostatic effect Effects 0.000 abstract 1
- 239000002812 cholic acid derivative Substances 0.000 abstract 1
- 239000011248 coating agent Substances 0.000 abstract 1
- 238000000576 coating method Methods 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 abstract 1
- 235000018417 cysteine Nutrition 0.000 abstract 1
- ZAKOWWREFLAJOT-UHFFFAOYSA-N d-alpha-Tocopheryl acetate Natural products CC(=O)OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-UHFFFAOYSA-N 0.000 abstract 1
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical class O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 abstract 1
- 235000014113 dietary fatty acids Nutrition 0.000 abstract 1
- POULHZVOKOAJMA-UHFFFAOYSA-N dodecanoic acid ester group Chemical class C(CCCCCCCCCCC)(=O)O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 abstract 1
- 239000003995 emulsifying agent Substances 0.000 abstract 1
- 150000002148 esters Chemical class 0.000 abstract 1
- 150000002170 ethers Chemical class 0.000 abstract 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 abstract 1
- 239000000194 fatty acid Substances 0.000 abstract 1
- 229930195729 fatty acid Natural products 0.000 abstract 1
- 150000004665 fatty acids Chemical class 0.000 abstract 1
- 150000002191 fatty alcohols Chemical class 0.000 abstract 1
- 239000003349 gelling agent Substances 0.000 abstract 1
- 235000010299 hexamethylene tetramine Nutrition 0.000 abstract 1
- 239000004312 hexamethylene tetramine Substances 0.000 abstract 1
- 229960004011 methenamine Drugs 0.000 abstract 1
- HQHBAGKIEAOSNM-UHFFFAOYSA-N naphtholphthalein Chemical compound C1=CC=C2C(C3(C4=CC=CC=C4C(=O)O3)C3=CC=C(C4=CC=CC=C43)O)=CC=C(O)C2=C1 HQHBAGKIEAOSNM-UHFFFAOYSA-N 0.000 abstract 1
- 239000003921 oil Substances 0.000 abstract 1
- 235000019198 oils Nutrition 0.000 abstract 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid group Chemical group C(CCCCCCC\C=C/CCCCCCCC)(=O)O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 abstract 1
- 235000008390 olive oil Nutrition 0.000 abstract 1
- 239000004006 olive oil Substances 0.000 abstract 1
- IPCSVZSSVZVIGE-UHFFFAOYSA-N palmitic acid group Chemical group C(CCCCCCCCCCCCCCC)(=O)O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 abstract 1
- 230000001717 pathogenic effect Effects 0.000 abstract 1
- 229920001223 polyethylene glycol Polymers 0.000 abstract 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 abstract 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 abstract 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 abstract 1
- 230000001681 protective effect Effects 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-N sulfonic acid Chemical class OS(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-N 0.000 abstract 1
- 229920003002 synthetic resin Polymers 0.000 abstract 1
- 239000000057 synthetic resin Substances 0.000 abstract 1
- 229940042585 tocopherol acetate Drugs 0.000 abstract 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
1,247,449. Determination of lipase; fat or fatty oil emulsion. MERCK. PATENT G.m.b.H. 8 Sept., 1969 [20 Sept., 1968; 27 Aug., 1969], No. 44330/69. Headings B1V and B1X. An agent for the determination of lipase in body fluids comprises an aqueous emulsion of a fat or fatty oil and a pH colour indicator which is one or .more of m-cresol purple, p-xylenolsulphophthalein, dibromothymolsulphophthalein, thymolsulphophthalein, alphanaphtholphthalein, which indicator may be present as 0.001 % to 1 % of the emulsion, optionally together with another colour indicator such as diphenol purple or a coloured compound such as carotin, in concentration up to 0.05%. The fat or oil may be natural or synthetic esters of fatty acids, e.g. tri- or di-glycerides of palmitic, stearic, oleic, linoleic, lauric acids, coconut fat, olive oil (preferred), hydrogenated rape seed oil. Optional components of the emulsion are 0.5 to 30% stabilizers, e.g. gum arabic, polyvinylpyrrolidone; up to 20% emulsifiers, e.g. polyethylene glycol fatty alcohol or alkylphenol ethers, polyoxyethylene derivatives of sorbitol anhydrides, sulphonic acid salts or sulphuric acid esters; up to 2% stabiliser and activator components, e.g. salts of bile acids such as alkali metal cholates, desoxycholates, taurocholates, glycocholates; up to 3% gelling agents, e.g., montmorillonite derivatives; up to 1 % viscosity - increasing agents, e.g. agar, anti-oxidants e.g. mercaptoethanol, cysteine, alphatocopherol acetate, ascorbyl palmitate; up to 1 % biostatic agents, e.g. organo-mercury compounds, p hydroxybenzoic acid esters, hexamethylene tetramine, iodacetates, azides. The indicator may be added as a solution to the emulsion or the solution and emulsion may be stored separately. Unit amounts of emulsion may be stored in synthetic resin squarebased cuvettes with a protective polymeric coating over the surface of the emulsion. In use, the agent is incubated with the sample and the colour developed is compared with a calibrated standard. Pathogenic lipase concentrations may be detected by comparison with results on normal body fluid samples.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19681798285 DE1798285C3 (en) | 1968-09-20 | New means and method for lipase determination | |
| DE19691943454 DE1943454A1 (en) | 1968-09-20 | 1969-08-27 | New means and method for lipasc determination |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1247449A true GB1247449A (en) | 1971-09-22 |
Family
ID=25756172
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB44330/69A Expired GB1247449A (en) | 1968-09-20 | 1969-09-08 | Agent for the determination of lipase |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US3689364A (en) |
| JP (1) | JPS5542703B1 (en) |
| BE (1) | BE738960A (en) |
| CH (1) | CH506792A (en) |
| DE (1) | DE1943454A1 (en) |
| FR (1) | FR2022180A1 (en) |
| GB (1) | GB1247449A (en) |
| IL (1) | IL32910A (en) |
| NL (1) | NL6913769A (en) |
| SE (1) | SE372631B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0050877A1 (en) * | 1980-10-28 | 1982-05-05 | Fuji Photo Film Co., Ltd. | Reagents for measuring lipase activity |
| CN100445731C (en) * | 2004-11-12 | 2008-12-24 | 中国科学院兰州化学物理研究所 | Method for colorimetric detecting and analysing cysteine |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3917515A (en) * | 1974-03-13 | 1975-11-04 | Jack M Goldberg | Serum lipase method and medium |
| US4045290A (en) * | 1975-02-28 | 1977-08-30 | Princeton Biomedix Incorporated | Diagnostic method and compounds for use therewith |
| CA1077498A (en) * | 1975-02-28 | 1980-05-13 | George F. Bulbenko | Thymol blue monophosphates and salts thereof |
| US4275152A (en) * | 1977-02-03 | 1981-06-23 | Eastman Kodak Company | Hydrolysis of protein-bound cholesterol esters |
| US4275151A (en) * | 1977-02-03 | 1981-06-23 | Eastman Kodak Company | Hydrolysis of protein-bound cholesterol esters |
| US4347313A (en) * | 1978-02-27 | 1982-08-31 | Boehringer Mannheim Gmbh | Analytical determination of lipase |
| DE2826965A1 (en) * | 1978-06-20 | 1980-01-17 | Boehringer Mannheim Gmbh | DIAGNOSTIC AGENT FOR DETECTING LEUCOCYTES IN BODY LIQUIDS AND CHROMOGENES SUITABLE FOR THIS |
| DE2905531A1 (en) * | 1979-02-14 | 1981-01-08 | Boehringer Mannheim Gmbh | DIAGNOSTIC AGENT FOR DETECTING LEUCOCYTES IN BODY LIQUIDS |
| EP0021572A1 (en) * | 1979-06-04 | 1981-01-07 | American Hospital Supply Corporation | A substrate for use in a turbidimetric assay for lipase and a method for making this substrate |
| US4555483A (en) * | 1982-08-11 | 1985-11-26 | Eastman Kodak Company | Methods, compositions and elements for the determination of lipase |
| DE3516001A1 (en) * | 1985-05-03 | 1986-11-06 | Boehringer Mannheim Gmbh, 6800 Mannheim | LIPASE COLOR TEST |
| TW442570B (en) * | 1996-09-19 | 2001-06-23 | Roche Diagnostics Gmbh | Improved colour test for the detection of activity of lipase |
| US5972595A (en) * | 1997-12-19 | 1999-10-26 | Nen Life Science Products, Inc. | Enzyme assay using a solid phase substrate |
-
1969
- 1969-07-25 CH CH1143969A patent/CH506792A/en not_active IP Right Cessation
- 1969-08-27 DE DE19691943454 patent/DE1943454A1/en active Pending
- 1969-08-28 IL IL32910A patent/IL32910A/en unknown
- 1969-09-08 GB GB44330/69A patent/GB1247449A/en not_active Expired
- 1969-09-10 NL NL6913769A patent/NL6913769A/xx unknown
- 1969-09-15 US US858154A patent/US3689364A/en not_active Expired - Lifetime
- 1969-09-16 SE SE6912716A patent/SE372631B/xx unknown
- 1969-09-17 BE BE738960A patent/BE738960A/xx unknown
- 1969-09-19 FR FR6931987A patent/FR2022180A1/fr not_active Withdrawn
- 1969-09-20 JP JP7439569A patent/JPS5542703B1/ja active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0050877A1 (en) * | 1980-10-28 | 1982-05-05 | Fuji Photo Film Co., Ltd. | Reagents for measuring lipase activity |
| CN100445731C (en) * | 2004-11-12 | 2008-12-24 | 中国科学院兰州化学物理研究所 | Method for colorimetric detecting and analysing cysteine |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5542703B1 (en) | 1980-11-01 |
| SE372631B (en) | 1974-12-23 |
| NL6913769A (en) | 1970-03-24 |
| BE738960A (en) | 1970-03-17 |
| US3689364A (en) | 1972-09-05 |
| IL32910A (en) | 1972-11-28 |
| IL32910A0 (en) | 1969-11-30 |
| DE1798285A1 (en) | 1972-01-20 |
| DE1943454A1 (en) | 1971-03-25 |
| FR2022180A1 (en) | 1970-07-31 |
| CH506792A (en) | 1971-04-30 |
| DE1798285B2 (en) | 1976-02-12 |
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