IL182340A - A method of isolating hepatoglobin from the V segment of human COHN - Google Patents
A method of isolating hepatoglobin from the V segment of human COHNInfo
- Publication number
- IL182340A IL182340A IL182340A IL18234007A IL182340A IL 182340 A IL182340 A IL 182340A IL 182340 A IL182340 A IL 182340A IL 18234007 A IL18234007 A IL 18234007A IL 182340 A IL182340 A IL 182340A
- Authority
- IL
- Israel
- Prior art keywords
- human
- substrate
- anion exchange
- loading
- cohn fraction
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract 39
- 108010058936 Cohn fraction V Proteins 0.000 title claims abstract 11
- 238000002955 isolation Methods 0.000 title claims abstract 3
- 102000014702 Haptoglobin Human genes 0.000 title abstract 3
- 108050005077 Haptoglobin Proteins 0.000 title abstract 3
- 241000700605 Viruses Species 0.000 claims abstract 4
- 230000002779 inactivation Effects 0.000 claims abstract 4
- 238000005571 anion exchange chromatography Methods 0.000 claims abstract 3
- 239000000758 substrate Substances 0.000 claims 12
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 9
- 239000000872 buffer Substances 0.000 claims 9
- 238000011068 loading method Methods 0.000 claims 8
- 238000005406 washing Methods 0.000 claims 8
- 238000005349 anion exchange Methods 0.000 claims 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 6
- 239000000356 contaminant Substances 0.000 claims 5
- 238000010828 elution Methods 0.000 claims 5
- 102000001708 Protein Isoforms Human genes 0.000 claims 4
- 108010029485 Protein Isoforms Proteins 0.000 claims 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims 3
- 239000003599 detergent Substances 0.000 claims 3
- 238000000746 purification Methods 0.000 claims 3
- 239000001632 sodium acetate Substances 0.000 claims 3
- 235000017281 sodium acetate Nutrition 0.000 claims 3
- 239000011780 sodium chloride Substances 0.000 claims 3
- 239000002904 solvent Substances 0.000 claims 3
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical group CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 claims 2
- 229920000936 Agarose Polymers 0.000 claims 2
- 102000009027 Albumins Human genes 0.000 claims 2
- 108010088751 Albumins Proteins 0.000 claims 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims 2
- 101001078385 Homo sapiens Haptoglobin Proteins 0.000 claims 2
- 238000004587 chromatography analysis Methods 0.000 claims 2
- 239000012149 elution buffer Substances 0.000 claims 2
- 238000010438 heat treatment Methods 0.000 claims 2
- 102000050796 human HP Human genes 0.000 claims 2
- 238000009928 pasteurization Methods 0.000 claims 2
- 239000000047 product Substances 0.000 claims 2
- 238000011100 viral filtration Methods 0.000 claims 2
- 239000011534 wash buffer Substances 0.000 claims 2
- 150000001413 amino acids Chemical class 0.000 claims 1
- 229960005070 ascorbic acid Drugs 0.000 claims 1
- 235000010323 ascorbic acid Nutrition 0.000 claims 1
- 239000011668 ascorbic acid Substances 0.000 claims 1
- 239000013522 chelant Substances 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 238000011026 diafiltration Methods 0.000 claims 1
- 239000012467 final product Substances 0.000 claims 1
- 238000011062 flow through chromatography Methods 0.000 claims 1
- 238000009472 formulation Methods 0.000 claims 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims 1
- 239000012160 loading buffer Substances 0.000 claims 1
- 229910052751 metal Inorganic materials 0.000 claims 1
- 239000002184 metal Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims 1
- 239000003381 stabilizer Substances 0.000 claims 1
- 235000000346 sugar Nutrition 0.000 claims 1
- 150000005846 sugar alcohols Chemical class 0.000 claims 1
- 150000008163 sugars Chemical class 0.000 claims 1
- 238000002560 therapeutic procedure Methods 0.000 claims 1
- 238000000108 ultra-filtration Methods 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- A61K38/1709—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- A61K38/1722—Plasma globulins, lactoglobulins
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4717—Plasma globulins, lactoglobulin
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Neurology (AREA)
- Pulmonology (AREA)
- Urology & Nephrology (AREA)
- Neurosurgery (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Steroid Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Semiconductor Lasers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0423196.5A GB0423196D0 (en) | 2004-10-19 | 2004-10-19 | Method |
| PCT/GB2005/004037 WO2006043062A1 (en) | 2004-10-19 | 2005-10-19 | Method for the isolation of haptoglobin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL182340A0 IL182340A0 (en) | 2007-07-24 |
| IL182340A true IL182340A (en) | 2012-06-28 |
Family
ID=33484812
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL182340A IL182340A (en) | 2004-10-19 | 2007-03-29 | A method of isolating hepatoglobin from the V segment of human COHN |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US9145448B2 (enExample) |
| EP (1) | EP1802654B1 (enExample) |
| JP (1) | JP4950053B2 (enExample) |
| AT (1) | ATE539085T1 (enExample) |
| AU (1) | AU2005297061B2 (enExample) |
| BR (1) | BRPI0516933B8 (enExample) |
| CA (1) | CA2584407C (enExample) |
| ES (1) | ES2379157T3 (enExample) |
| GB (1) | GB0423196D0 (enExample) |
| IL (1) | IL182340A (enExample) |
| MX (1) | MX2007004244A (enExample) |
| PL (1) | PL1802654T3 (enExample) |
| WO (1) | WO2006043062A1 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0423196D0 (en) | 2004-10-19 | 2004-11-24 | Nat Blood Authority | Method |
| US9534029B2 (en) | 2012-10-03 | 2017-01-03 | Csl Behring Ag | Method of purifying proteins |
| HK1212995A1 (zh) * | 2012-10-03 | 2016-06-24 | Csl Behring Ag | 一种纯化蛋白质的方法 |
| EP3917943A4 (en) * | 2019-02-01 | 2022-12-07 | Ohio State Innovation Foundation | PROTEINS PURIFICATION PROCEDURES |
| US20220218834A1 (en) * | 2019-05-20 | 2022-07-14 | Ohio State Innovation Foundation | Apohemoglobin-haptoglobin complexes and methods of using thereof |
| US20240034749A1 (en) * | 2021-02-04 | 2024-02-01 | Green Cross Corporation | Method for purification of hemopexin and haptoglobin |
| CN115531921B (zh) * | 2022-08-26 | 2024-07-16 | 大连依利特分析仪器有限公司 | 一种制备型液相色谱馏分收集装置及方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4061735A (en) * | 1973-11-15 | 1977-12-06 | The Green Cross Corporation | Haptoglobin in aqueous solution and process for preparing the same |
| GB1426039A (en) | 1973-11-15 | 1976-02-25 | Green Cross Corp | Haptoglobin in aqueous solution and process for tits preparation |
| US4137307A (en) * | 1973-11-15 | 1979-01-30 | The Green Cross Corporation | Process for preparing haptoglobin aqueous solution using strong anion exchanger |
| JPS5337406B2 (enExample) * | 1973-11-15 | 1978-10-09 | ||
| JPS52128205A (en) * | 1976-04-16 | 1977-10-27 | Green Cross Corp:The | Prophylactic and therapeutic drugs for cerebro-vascular contraction |
| US4540573A (en) | 1983-07-14 | 1985-09-10 | New York Blood Center, Inc. | Undenatured virus-free biologically active protein derivatives |
| US5094960A (en) * | 1988-10-07 | 1992-03-10 | New York Blood Center, Inc. | Removal of process chemicals from labile biological mixtures by hydrophobic interaction chromatography |
| JP3554796B2 (ja) * | 1988-10-31 | 2004-08-18 | 三菱ウェルファーマ株式会社 | アルブミン製剤及びその製造方法 |
| JP3825061B2 (ja) * | 1994-09-28 | 2006-09-20 | 三菱ウェルファーマ株式会社 | ハプトグロビンの製造方法 |
| DE19960500A1 (de) * | 1999-12-15 | 2001-07-12 | Giesing Michael | Antikörper und Verfahren zu ihrer Herstellung, deren Verwendung sowie Immunisierungscocktails, Immunoassay-Sets und Peptide |
| PT1328297E (pt) * | 2000-10-16 | 2010-01-04 | Cytoguide Aps | Função de um receptor de haptoglobina-hemoglobina e suas utilizações |
| US20030113830A1 (en) | 2001-07-11 | 2003-06-19 | Rappaport Family Institute For Research In The Medical Sciences | Novel antioxidant, nucleic acid constructs encoding same, pharmaceutical compositions containing same and use of same for reducing oxidative-stress |
| JP2004244348A (ja) * | 2003-02-13 | 2004-09-02 | Nihon Pharmaceutical Co Ltd | パプトグロビンの精製法 |
| GB0423196D0 (en) | 2004-10-19 | 2004-11-24 | Nat Blood Authority | Method |
| WO2006107708A1 (en) | 2005-04-04 | 2006-10-12 | Alexion Pharmaceuticals, Inc. | Treatment of disease caused by excess free hemoglobin |
-
2004
- 2004-10-19 GB GBGB0423196.5A patent/GB0423196D0/en not_active Ceased
-
2005
- 2005-10-19 US US11/577,478 patent/US9145448B2/en active Active
- 2005-10-19 CA CA2584407A patent/CA2584407C/en not_active Expired - Lifetime
- 2005-10-19 ES ES05794729T patent/ES2379157T3/es not_active Expired - Lifetime
- 2005-10-19 BR BRPI0516933A patent/BRPI0516933B8/pt active IP Right Grant
- 2005-10-19 AU AU2005297061A patent/AU2005297061B2/en not_active Ceased
- 2005-10-19 MX MX2007004244A patent/MX2007004244A/es active IP Right Grant
- 2005-10-19 WO PCT/GB2005/004037 patent/WO2006043062A1/en not_active Ceased
- 2005-10-19 EP EP05794729A patent/EP1802654B1/en not_active Expired - Lifetime
- 2005-10-19 JP JP2007537377A patent/JP4950053B2/ja not_active Expired - Lifetime
- 2005-10-19 AT AT05794729T patent/ATE539085T1/de active
- 2005-10-19 PL PL05794729T patent/PL1802654T3/pl unknown
-
2007
- 2007-03-29 IL IL182340A patent/IL182340A/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| BRPI0516933B8 (pt) | 2021-05-25 |
| JP4950053B2 (ja) | 2012-06-13 |
| BRPI0516933A (pt) | 2008-09-23 |
| ES2379157T3 (es) | 2012-04-23 |
| IL182340A0 (en) | 2007-07-24 |
| EP1802654B1 (en) | 2011-12-28 |
| AU2005297061A1 (en) | 2006-04-27 |
| EP1802654A1 (en) | 2007-07-04 |
| MX2007004244A (es) | 2007-09-07 |
| JP2008517046A (ja) | 2008-05-22 |
| CA2584407C (en) | 2015-02-03 |
| AU2005297061B2 (en) | 2011-09-01 |
| US20090281282A1 (en) | 2009-11-12 |
| BRPI0516933B1 (pt) | 2021-02-09 |
| CA2584407A1 (en) | 2006-04-27 |
| GB0423196D0 (en) | 2004-11-24 |
| WO2006043062A1 (en) | 2006-04-27 |
| ATE539085T1 (de) | 2012-01-15 |
| US9145448B2 (en) | 2015-09-29 |
| PL1802654T3 (pl) | 2012-05-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FF | Patent granted | ||
| KB | Patent renewed | ||
| KB | Patent renewed | ||
| KB | Patent renewed |