IL127764A - Purified sperm surface antigen, monoclonal antibody therfor and applications therefor - Google Patents
Purified sperm surface antigen, monoclonal antibody therfor and applications thereforInfo
- Publication number
- IL127764A IL127764A IL12776497A IL12776497A IL127764A IL 127764 A IL127764 A IL 127764A IL 12776497 A IL12776497 A IL 12776497A IL 12776497 A IL12776497 A IL 12776497A IL 127764 A IL127764 A IL 127764A
- Authority
- IL
- Israel
- Prior art keywords
- sperm
- antibody
- bound
- glycoprotein
- protein
- Prior art date
Links
- 102000036639 antigens Human genes 0.000 title description 42
- 108091007433 antigens Proteins 0.000 title description 42
- 239000000427 antigen Substances 0.000 title description 41
- 210000004408 hybridoma Anatomy 0.000 claims abstract description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 62
- 108090000623 proteins and genes Proteins 0.000 claims description 62
- 229960005486 vaccine Drugs 0.000 claims description 21
- 239000000934 spermatocidal agent Substances 0.000 claims description 19
- 210000004027 cell Anatomy 0.000 claims description 14
- 230000004720 fertilization Effects 0.000 claims description 13
- 108090000288 Glycoproteins Proteins 0.000 claims description 12
- 102000003886 Glycoproteins Human genes 0.000 claims description 12
- 230000005764 inhibitory process Effects 0.000 claims description 11
- 229940124462 contraceptive vaccine Drugs 0.000 claims description 10
- 239000002502 liposome Substances 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 9
- 230000035558 fertility Effects 0.000 claims description 6
- 238000002255 vaccination Methods 0.000 claims description 6
- 239000003937 drug carrier Substances 0.000 claims description 4
- 150000003904 phospholipids Chemical class 0.000 claims description 4
- 239000013543 active substance Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 108010090054 Membrane Glycoproteins Proteins 0.000 claims description 2
- 102000012750 Membrane Glycoproteins Human genes 0.000 claims description 2
- 241000124008 Mammalia Species 0.000 claims 2
- 238000006243 chemical reaction Methods 0.000 description 19
- 239000003599 detergent Substances 0.000 description 16
- 230000002163 immunogen Effects 0.000 description 14
- 238000012360 testing method Methods 0.000 description 14
- 239000000284 extract Substances 0.000 description 13
- 239000000203 mixture Substances 0.000 description 13
- 239000012071 phase Substances 0.000 description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 239000002299 complementary DNA Substances 0.000 description 12
- 239000000499 gel Substances 0.000 description 12
- IDOQDZANRZQBTP-UHFFFAOYSA-N 2-[2-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=CC=C1OCCO IDOQDZANRZQBTP-UHFFFAOYSA-N 0.000 description 11
- 229920004929 Triton X-114 Polymers 0.000 description 11
- 239000011324 bead Substances 0.000 description 11
- 239000003153 chemical reaction reagent Substances 0.000 description 11
- 238000011161 development Methods 0.000 description 11
- 239000003981 vehicle Substances 0.000 description 11
- 239000000872 buffer Substances 0.000 description 10
- 206010003445 Ascites Diseases 0.000 description 9
- 230000004520 agglutination Effects 0.000 description 9
- 238000003556 assay Methods 0.000 description 9
- 239000012634 fragment Substances 0.000 description 9
- HEGSGKPQLMEBJL-RKQHYHRCSA-N octyl beta-D-glucopyranoside Chemical compound CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HEGSGKPQLMEBJL-RKQHYHRCSA-N 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 238000000746 purification Methods 0.000 description 7
- 239000013598 vector Substances 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 108010052285 Membrane Proteins Proteins 0.000 description 6
- 102000018697 Membrane Proteins Human genes 0.000 description 6
- 229920002684 Sepharose Polymers 0.000 description 6
- 238000010367 cloning Methods 0.000 description 6
- 239000003433 contraceptive agent Substances 0.000 description 6
- 230000002254 contraceptive effect Effects 0.000 description 6
- 239000013612 plasmid Substances 0.000 description 6
- 230000009257 reactivity Effects 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 108091034057 RNA (poly(A)) Proteins 0.000 description 5
- 238000007792 addition Methods 0.000 description 5
- 239000008346 aqueous phase Substances 0.000 description 5
- 230000029087 digestion Effects 0.000 description 5
- 230000002209 hydrophobic effect Effects 0.000 description 5
- 239000000693 micelle Substances 0.000 description 5
- 239000008188 pellet Substances 0.000 description 5
- 108091008146 restriction endonucleases Proteins 0.000 description 5
- 238000012163 sequencing technique Methods 0.000 description 5
- 238000000638 solvent extraction Methods 0.000 description 5
- 238000001262 western blot Methods 0.000 description 5
- 108091034117 Oligonucleotide Proteins 0.000 description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 4
- 229940044197 ammonium sulfate Drugs 0.000 description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 4
- 235000011130 ammonium sulphate Nutrition 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000011347 resin Substances 0.000 description 4
- 229920005989 resin Polymers 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 230000000699 topical effect Effects 0.000 description 4
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 3
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 3
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 3
- 241000288906 Primates Species 0.000 description 3
- 238000012300 Sequence Analysis Methods 0.000 description 3
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
- 230000001594 aberrant effect Effects 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 238000007818 agglutination assay Methods 0.000 description 3
- 238000002820 assay format Methods 0.000 description 3
- 238000003149 assay kit Methods 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229940084986 human chorionic gonadotropin Drugs 0.000 description 3
- 238000002649 immunization Methods 0.000 description 3
- 230000003053 immunization Effects 0.000 description 3
- 238000003119 immunoblot Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 229940031348 multivalent vaccine Drugs 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 239000011535 reaction buffer Substances 0.000 description 3
- 229910052709 silver Inorganic materials 0.000 description 3
- 239000004332 silver Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 3
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- HKJKONMZMPUGHJ-UHFFFAOYSA-N 4-amino-5-hydroxy-3-[(4-nitrophenyl)diazenyl]-6-phenyldiazenylnaphthalene-2,7-disulfonic acid Chemical compound OS(=O)(=O)C1=CC2=CC(S(O)(=O)=O)=C(N=NC=3C=CC=CC=3)C(O)=C2C(N)=C1N=NC1=CC=C([N+]([O-])=O)C=C1 HKJKONMZMPUGHJ-UHFFFAOYSA-N 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 108010067770 Endopeptidase K Proteins 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 241001529936 Murinae Species 0.000 description 2
- 108010002747 Pfu DNA polymerase Proteins 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 230000003509 anti-fertility effect Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 210000004379 membrane Anatomy 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 229940127234 oral contraceptive Drugs 0.000 description 2
- 239000003539 oral contraceptive agent Substances 0.000 description 2
- 230000035515 penetration Effects 0.000 description 2
- 230000000737 periodic effect Effects 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 238000003757 reverse transcription PCR Methods 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 101710186708 Agglutinin Proteins 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000606643 Anaplasma centrale Species 0.000 description 1
- 101710145634 Antigen 1 Proteins 0.000 description 1
- 208000002109 Argyria Diseases 0.000 description 1
- 101100462495 Caenorhabditis elegans rsa-1 gene Proteins 0.000 description 1
- 238000009007 Diagnostic Kit Methods 0.000 description 1
- 102000001133 Fertilins Human genes 0.000 description 1
- 108010069446 Fertilins Proteins 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101710146024 Horcolin Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical group O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- 108010028554 LDL Cholesterol Proteins 0.000 description 1
- 101710189395 Lectin Proteins 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 101710179758 Mannose-specific lectin Proteins 0.000 description 1
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 description 1
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 101100310691 Rattus norvegicus Spata6 gene Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 101710120037 Toxin CcdB Proteins 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 206010000210 abortion Diseases 0.000 description 1
- 231100000176 abortion Toxicity 0.000 description 1
- 238000010306 acid treatment Methods 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 239000000910 agglutinin Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000000469 anti-sperm effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 125000000837 carbohydrate group Chemical group 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 210000003756 cervix mucus Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000009223 counseling Methods 0.000 description 1
- 239000007822 coupling agent Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 239000005546 dideoxynucleotide Substances 0.000 description 1
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
- LRHXBHUTQWIZTN-UHFFFAOYSA-N dimethyl heptanediimidate;dihydrochloride Chemical compound Cl.Cl.COC(=N)CCCCCC(=N)OC LRHXBHUTQWIZTN-UHFFFAOYSA-N 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 229960003085 meticillin Drugs 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229940087419 nonoxynol-9 Drugs 0.000 description 1
- 229920004918 nonoxynol-9 Polymers 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229920002113 octoxynol Polymers 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 238000011809 primate model Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000036647 reaction Effects 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000011808 rodent model Methods 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000019100 sperm motility Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- FRGKKTITADJNOE-UHFFFAOYSA-N sulfanyloxyethane Chemical compound CCOS FRGKKTITADJNOE-UHFFFAOYSA-N 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- FBWNMEQMRUMQSO-UHFFFAOYSA-N tergitol NP-9 Chemical compound CCCCCCCCCC1=CC=C(OCCOCCOCCOCCOCCOCCOCCOCCOCCO)C=C1 FBWNMEQMRUMQSO-UHFFFAOYSA-N 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000036964 tight binding Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- 238000007879 vasectomy Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 238000010865 video microscopy Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 210000004340 zona pellucida Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/16—Masculine contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/18—Feminine contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/36—Gynecology or obstetrics
- G01N2800/367—Infertility, e.g. sperm disorder, ovulatory dysfunction
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Reproductive Health (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Cell Biology (AREA)
- Endocrinology (AREA)
- Gynecology & Obstetrics (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/671,622 US5830472A (en) | 1996-06-28 | 1996-06-28 | Purified sperm surface antigen, monoclonal antibody therefor and applications therefor |
| PCT/US1997/010813 WO1998000164A1 (en) | 1996-06-28 | 1997-06-30 | Purified sperm surface antigen, monoclonal antibody therefor and applications therefor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL127764A0 IL127764A0 (en) | 1999-10-28 |
| IL127764A true IL127764A (en) | 2001-11-25 |
Family
ID=24695254
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL12776497A IL127764A (en) | 1996-06-28 | 1997-06-30 | Purified sperm surface antigen, monoclonal antibody therfor and applications therefor |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US5830472A (de) |
| EP (1) | EP0964696A4 (de) |
| JP (1) | JP2001506841A (de) |
| CN (1) | CN1227497A (de) |
| AU (1) | AU722559B2 (de) |
| BR (1) | BR9710089A (de) |
| CA (1) | CA2258717A1 (de) |
| CZ (1) | CZ293040B6 (de) |
| EA (1) | EA003531B1 (de) |
| HU (1) | HUP9904620A3 (de) |
| IL (1) | IL127764A (de) |
| NO (1) | NO986050L (de) |
| PL (1) | PL186809B1 (de) |
| SK (1) | SK281505B6 (de) |
| UA (1) | UA74765C2 (de) |
| WO (1) | WO1998000164A1 (de) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002539223A (ja) * | 1999-03-23 | 2002-11-19 | ユニバーシティ オブ バージニア パテント ファウンデーション | ヒト精子表面抗原 |
| GB9913556D0 (en) * | 1999-06-11 | 1999-08-11 | Zeneca Ltd | Assays |
| AU6227800A (en) * | 1999-07-23 | 2001-02-13 | University Of Virginia Patent Foundation | Recombinant antibody directed against human sperm antigen |
| US20020151089A1 (en) * | 1999-09-15 | 2002-10-17 | Chapman William H. | Separating components of biological samples |
| WO2001035759A1 (en) * | 1999-11-17 | 2001-05-25 | University Of Virginia Patent Foundation | Sperm cell selection system |
| EP1255831A2 (de) | 2000-01-19 | 2002-11-13 | The University Of Virginia Patent Foundation | Sperma spezifische proteine |
| US7125550B2 (en) * | 2000-01-19 | 2006-10-24 | University Of Virginia Patent Foundation | Human sperm specific lysozyme-like proteins |
| EP1261706A2 (de) | 2000-01-20 | 2002-12-04 | University Of Virginia Patent Foundation | Proteine spezifisch für die oberfläche von oozyten |
| US6541206B1 (en) * | 2000-06-19 | 2003-04-01 | Gabor B. Huszar | Objective biochemical method for assessment of sperm quality |
| US20030207393A1 (en) * | 2001-01-19 | 2003-11-06 | Herr John C. | Cbp86, a sperm specific protein |
| US20080318250A1 (en) * | 2004-02-06 | 2008-12-25 | Linda Gilmer | Compositions and Methods for Identifying Sperm for Forensic Applications |
| US8817965B2 (en) | 2006-07-21 | 2014-08-26 | Bce Inc. | Method, system and apparatus for handling establishment of a communication session |
| US20080187549A1 (en) * | 2007-02-07 | 2008-08-07 | Morey William A | Polyantigenic-based (multiple antigens) modi or set of methods for developing infertility vaccines |
| US20080290037A1 (en) * | 2007-05-23 | 2008-11-27 | Applera Corporation | Methods and Apparatuses for Separating Biological Particles |
| US20090136962A1 (en) * | 2007-11-27 | 2009-05-28 | Herr John C | Diagnostic markers for cancer |
| ES2394035T3 (es) * | 2008-04-07 | 2013-01-15 | Auburn University | Métodos para inmunocontracepción específica para ciertas especies de animales |
| GB0911885D0 (en) * | 2009-07-08 | 2009-08-19 | Oxford Biodynamics Ltd | Screening method for cell aging |
| IL208820A0 (en) | 2010-10-19 | 2011-01-31 | Rachel Teitelbaum | Biologic female contraceptives |
| WO2014202978A1 (en) | 2013-06-18 | 2014-12-24 | Isis Innovation Limited | Method of detecting the presence or absence of autoantibodies |
| CN103852583A (zh) * | 2014-03-18 | 2014-06-11 | 南京医科大学 | 一种快速检测Boule蛋白的胶体金试剂条及检测方法 |
| CN106153938B (zh) * | 2015-04-09 | 2018-05-11 | 中国医学科学院基础医学研究所 | 抗ACTL7a和GAPDH-2抗体的检测方法、试剂盒及其用途 |
| KR101916959B1 (ko) * | 2015-07-13 | 2018-11-08 | 김동구 | 정자의 성 감별용 항체 및 이의 용도 |
| CN106526162A (zh) * | 2016-11-03 | 2017-03-22 | 天津市宝坻区人民医院 | 一种抗精子抗体检测方法 |
| CN117987376B (zh) * | 2024-03-26 | 2024-06-25 | 玛特瑞尔(吉林)生物技术有限公司 | 人精子顶体蛋白sp-10单克隆抗体、杂交瘤细胞株及其应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5227160A (en) * | 1988-07-15 | 1993-07-13 | The Biomembrane Institute | Monoclonal antibody nuh2 capable of inactivating motility of human sperm, antigen defined by said monoclonal antibody and methods of using said monoclonal antibody and antigen |
| US5605803A (en) * | 1989-03-03 | 1997-02-25 | University Of Virginia Alumni Patents Foundation | Human sperm diagnostic |
| US5436157A (en) * | 1989-03-03 | 1995-07-25 | The University Of Virginia Alumni Patents Foundation | Human intra-acrosomal sperm antigen |
-
1996
- 1996-06-28 US US08/671,622 patent/US5830472A/en not_active Expired - Fee Related
-
1997
- 1997-06-30 CN CN97197175A patent/CN1227497A/zh active Pending
- 1997-06-30 IL IL12776497A patent/IL127764A/en not_active IP Right Cessation
- 1997-06-30 SK SK1795-98A patent/SK281505B6/sk unknown
- 1997-06-30 BR BR9710089-7A patent/BR9710089A/pt not_active Application Discontinuation
- 1997-06-30 WO PCT/US1997/010813 patent/WO1998000164A1/en not_active Application Discontinuation
- 1997-06-30 EP EP97930179A patent/EP0964696A4/de not_active Withdrawn
- 1997-06-30 EA EA199900067A patent/EA003531B1/ru unknown
- 1997-06-30 AU AU34071/97A patent/AU722559B2/en not_active Ceased
- 1997-06-30 UA UA99010427A patent/UA74765C2/uk unknown
- 1997-06-30 CZ CZ19984284A patent/CZ293040B6/cs not_active IP Right Cessation
- 1997-06-30 HU HU9904620A patent/HUP9904620A3/hu unknown
- 1997-06-30 PL PL97330842A patent/PL186809B1/pl not_active IP Right Cessation
- 1997-06-30 JP JP50421698A patent/JP2001506841A/ja not_active Ceased
- 1997-06-30 CA CA002258717A patent/CA2258717A1/en not_active Abandoned
-
1998
- 1998-12-22 NO NO986050A patent/NO986050L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| CZ428498A3 (cs) | 1999-06-16 |
| BR9710089A (pt) | 2000-06-06 |
| IL127764A0 (en) | 1999-10-28 |
| CN1227497A (zh) | 1999-09-01 |
| EA199900067A1 (ru) | 1999-08-26 |
| US5830472A (en) | 1998-11-03 |
| EP0964696A4 (de) | 2005-01-05 |
| UA74765C2 (en) | 2006-02-15 |
| NO986050L (no) | 1999-02-08 |
| SK179598A3 (en) | 2000-04-10 |
| EA003531B1 (ru) | 2003-06-26 |
| HUP9904620A3 (en) | 2001-08-28 |
| CA2258717A1 (en) | 1998-01-08 |
| CZ293040B6 (cs) | 2004-01-14 |
| PL330842A1 (en) | 1999-06-07 |
| JP2001506841A (ja) | 2001-05-29 |
| WO1998000164A1 (en) | 1998-01-08 |
| AU722559B2 (en) | 2000-08-03 |
| AU3407197A (en) | 1998-01-21 |
| PL186809B1 (pl) | 2004-02-27 |
| SK281505B6 (sk) | 2001-04-09 |
| HUP9904620A2 (hu) | 2000-05-28 |
| NO986050D0 (no) | 1998-12-22 |
| EP0964696A1 (de) | 1999-12-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU722559B2 (en) | Purified sperm surface antigen, monoclonal antibody therefor and applications therefor | |
| Diekman et al. | Sperm antigens and their use in the development of an immunocontraceptive | |
| Zou et al. | Detection of anti-MICA antibodies in patients awaiting kidney transplantation, during the post-transplant course, and in eluates from rejected kidney allografts by Luminex flow cytometry | |
| Herr et al. | Biochemical and morphological characterization of the intra-acrosomal antigen SP-1O from human sperm | |
| Glassock | The pathogenesis of idiopathic membranous nephropathy: a 50-year odyssey | |
| Naz et al. | Identification of Human Sperm Peptide Sequence Involved in Egg Bindingfor Immunocontraception | |
| JP2001503601A (ja) | 前立腺特異的膜抗原の細胞外ドメインに対して特異的なモノクローナル抗体 | |
| Naz | Vaccine for human contraception targeting sperm Izumo protein and YLP12 dodecamer peptide | |
| US6258364B1 (en) | Purified sperm surface antigen, monoclonal antibody therefor and application therefor | |
| US8012932B2 (en) | Contraceptives based on SP22 and SP22 antibodies | |
| CN108567973B (zh) | 一种胎盘样硫酸软骨素a免疫组合物及应用 | |
| JPH10501970A (ja) | 避妊ワクチン | |
| Kurth et al. | Immunogenicity of a multi-component recombinant human acrosomal protein vaccine in female Macaca fascicularis | |
| US20130273088A1 (en) | Epitopes of herpes simplex virus | |
| Diekman et al. | Evidence for a unique N‐linked glycan associated with human infertility on sperm CD52: a candidate contraceptive vaccinogen | |
| Clayton et al. | A combinatorial phage display library for the generation of specific Fab fragments recognizing human spermatozoa and inhibiting fertilizing capacity in vitro | |
| KR100513186B1 (ko) | 정제된정자표면항원,그의모노클로날항체및응용 | |
| US20150087000A1 (en) | Diagnostic kits to detect sp22 and sp22 antibodies | |
| JP2003520594A (ja) | 精子特異的タンパク質 | |
| An et al. | In vitro and in vivo studies evaluating recombinant plasmid pCXN2‐mIzumo as a potential immunocontraceptive antigen | |
| MXPA99000257A (en) | Superficial antigen of purified esperam, your monoclonal antibody and its application | |
| Shibahara | Development of Immunocontraceptives in Female | |
| JP2001514743A (ja) | 細胞表面タンパク質に対するワクチンの製造方法 | |
| JP3532945B2 (ja) | 抗ヒトチロシナーゼモノクローナル抗体 | |
| ES2626491B1 (es) | Anticuerpos monoclonales frente a bambi y uso para tratamiento de enfermedades inflamatorias |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| KB | Patent renewed | ||
| KB | Patent renewed | ||
| MM9K | Patent not in force due to non-payment of renewal fees |