HUE028309T2 - Cytological or histological fixation preparations and staining procedures - Google Patents
Cytological or histological fixation preparations and staining procedures Download PDFInfo
- Publication number
- HUE028309T2 HUE028309T2 HUE10752066A HUE10752066A HUE028309T2 HU E028309 T2 HUE028309 T2 HU E028309T2 HU E10752066 A HUE10752066 A HU E10752066A HU E10752066 A HUE10752066 A HU E10752066A HU E028309 T2 HUE028309 T2 HU E028309T2
- Authority
- HU
- Hungary
- Prior art keywords
- solution
- composition
- colouring
- blue
- alcohol
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims description 54
- 230000002380 cytological effect Effects 0.000 title 1
- 238000007447 staining method Methods 0.000 title 1
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical class OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 66
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 54
- 238000000034 method Methods 0.000 claims description 49
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 24
- 239000008280 blood Substances 0.000 claims description 24
- 210000004369 blood Anatomy 0.000 claims description 24
- 239000000975 dye Substances 0.000 claims description 16
- 238000002360 preparation method Methods 0.000 claims description 15
- 229960000907 methylthioninium chloride Drugs 0.000 claims description 12
- 239000011780 sodium chloride Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 239000001045 blue dye Substances 0.000 claims description 9
- 239000001044 red dye Substances 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 6
- 239000000872 buffer Substances 0.000 claims description 5
- 125000000325 methylidene group Chemical group [H]C([H])=* 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 2
- 239000003086 colorant Substances 0.000 claims description 2
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 claims description 2
- 238000010186 staining Methods 0.000 claims 3
- 238000004043 dyeing Methods 0.000 claims 2
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 claims 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims 1
- 239000004472 Lysine Substances 0.000 claims 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- IRERQBUNZFJFGC-UHFFFAOYSA-L azure blue Chemical compound [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Al+3].[Al+3].[Al+3].[Al+3].[Al+3].[Al+3].[S-]S[S-].[O-][Si]([O-])([O-])[O-].[O-][Si]([O-])([O-])[O-].[O-][Si]([O-])([O-])[O-].[O-][Si]([O-])([O-])[O-].[O-][Si]([O-])([O-])[O-].[O-][Si]([O-])([O-])[O-] IRERQBUNZFJFGC-UHFFFAOYSA-L 0.000 claims 1
- 230000001413 cellular effect Effects 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 claims 1
- 210000003238 esophagus Anatomy 0.000 claims 1
- 239000000834 fixative Substances 0.000 claims 1
- 239000000796 flavoring agent Substances 0.000 claims 1
- 238000011010 flushing procedure Methods 0.000 claims 1
- 238000009472 formulation Methods 0.000 claims 1
- 239000000976 ink Substances 0.000 claims 1
- 229910052744 lithium Inorganic materials 0.000 claims 1
- 239000008188 pellet Substances 0.000 claims 1
- 239000011574 phosphorus Substances 0.000 claims 1
- 229910052698 phosphorus Inorganic materials 0.000 claims 1
- 238000004040 coloring Methods 0.000 description 75
- 210000004027 cell Anatomy 0.000 description 30
- 239000000243 solution Substances 0.000 description 30
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 18
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 14
- 239000003153 chemical reaction reagent Substances 0.000 description 13
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 9
- 238000010191 image analysis Methods 0.000 description 9
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 6
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 6
- 229910000397 disodium phosphate Inorganic materials 0.000 description 6
- 235000019800 disodium phosphate Nutrition 0.000 description 6
- DNDJEIWCTMMZBX-UHFFFAOYSA-N n,n-dimethyl-7-methyliminophenothiazin-3-amine;hydrochloride Chemical compound [Cl-].C1=CC(=[N+](C)C)C=C2SC3=CC(NC)=CC=C3N=C21 DNDJEIWCTMMZBX-UHFFFAOYSA-N 0.000 description 6
- 239000001488 sodium phosphate Substances 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 5
- 239000011734 sodium Substances 0.000 description 5
- 229910052708 sodium Inorganic materials 0.000 description 5
- 231100000331 toxic Toxicity 0.000 description 5
- 230000002588 toxic effect Effects 0.000 description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
- 150000001298 alcohols Chemical class 0.000 description 3
- 239000004599 antimicrobial Substances 0.000 description 3
- 210000003850 cellular structure Anatomy 0.000 description 3
- SEACYXSIPDVVMV-UHFFFAOYSA-L eosin Y Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C([O-])=C(Br)C=C21 SEACYXSIPDVVMV-UHFFFAOYSA-L 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 238000010304 firing Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 235000008694 Humulus lupulus Nutrition 0.000 description 2
- 244000025221 Humulus lupulus Species 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 230000008021 deposition Effects 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002736 nonionic surfactant Substances 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 235000021317 phosphate Nutrition 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 238000004382 potting Methods 0.000 description 2
- 239000012487 rinsing solution Substances 0.000 description 2
- AGIJRRREJXSQJR-UHFFFAOYSA-N 2h-thiazine Chemical group N1SC=CC=C1 AGIJRRREJXSQJR-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical group ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 101100281518 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) fox-2 gene Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- GRXKLBBBQUKJJZ-UHFFFAOYSA-N Soman Chemical compound CC(C)(C)C(C)OP(C)(F)=O GRXKLBBBQUKJJZ-UHFFFAOYSA-N 0.000 description 1
- RUXQWZJWMCHCHH-IZZDOVSWSA-N [(e)-1-pyridin-2-ylethylideneamino]urea Chemical compound NC(=O)N\N=C(/C)C1=CC=CC=N1 RUXQWZJWMCHCHH-IZZDOVSWSA-N 0.000 description 1
- YOWZJZJLXUQHGF-UHFFFAOYSA-M [7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;dimethyl-[7-(methylamino)phenothiazin-3-ylidene]azanium;dichloride Chemical compound [Cl-].[Cl-].C1=CC(=[N+](C)C)C=C2SC3=CC(NC)=CC=C3N=C21.C1=CC(=[N+](C)C)C=C2SC3=CC(N(C)C)=CC=C3N=C21 YOWZJZJLXUQHGF-UHFFFAOYSA-M 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 230000005859 cell recognition Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- -1 dlmethylsuifoxide Chemical compound 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 230000036651 mood Effects 0.000 description 1
- SYQBFIAQOQZEGI-UHFFFAOYSA-N osmium atom Chemical compound [Os] SYQBFIAQOQZEGI-UHFFFAOYSA-N 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 1
- 239000008279 sol Substances 0.000 description 1
- 125000001990 thiamine group Chemical group 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0955127A FR2948456B1 (fr) | 2009-07-22 | 2009-07-22 | Composition de fixation cytologique ou histologique et procedes de coloration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HUE028309T2 true HUE028309T2 (en) | 2016-12-28 |
Family
ID=41722889
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HUE10752066A HUE028309T2 (en) | 2009-07-22 | 2010-07-21 | Cytological or histological fixation preparations and staining procedures |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US9366604B2 (enExample) |
| EP (1) | EP2457079B1 (enExample) |
| JP (1) | JP5704466B2 (enExample) |
| BR (1) | BR112012001495B1 (enExample) |
| ES (1) | ES2555219T3 (enExample) |
| FR (1) | FR2948456B1 (enExample) |
| HU (1) | HUE028309T2 (enExample) |
| IN (1) | IN2012DN01386A (enExample) |
| RU (1) | RU2536502C2 (enExample) |
| WO (1) | WO2011010064A1 (enExample) |
| ZA (1) | ZA201201238B (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2839542A1 (en) | 2011-06-17 | 2012-12-20 | Constitution Medical, Inc. | Fixative and staining solutions |
| US20160291045A1 (en) | 2015-04-06 | 2016-10-06 | Nanocytomics, LLC | Automated specimen deposition systems and associated methods |
| RU2630983C1 (ru) * | 2016-04-06 | 2017-09-15 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный медицинский университет" Министерства здравоохранения Российской Федерации (ФГБОУ ВО КубГМУ Минздрава России) | Фиксатор проб для цитологических исследований |
| CN112781961A (zh) * | 2019-11-07 | 2021-05-11 | 北京望升伟业科技发展有限公司 | 一种细胞快速染色液及其应用 |
| DE102022129378B3 (de) | 2022-11-07 | 2024-05-08 | Bio-Gram Diagnostics GmbH | Färbereagenz |
| WO2024099595A1 (en) * | 2022-11-07 | 2024-05-16 | Bio-Gram Diagnostics GmbH | Staining reagent |
| CN115855607A (zh) * | 2022-12-13 | 2023-03-28 | 湖南友哲科技有限公司 | 一种尿有形成分分析仪用染色液及其制备方法与使用方法 |
| DE102024105392A1 (de) * | 2024-02-27 | 2025-08-28 | Bio-Gram Hematology GmbH | Färbereagenz |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5260048A (en) * | 1991-05-08 | 1993-11-09 | Streck Laboratories, Inc. | Tissue fixative solution and method |
| WO1994007532A1 (en) * | 1992-09-10 | 1994-04-14 | Streck Laboratories, Inc. | Method and composition for preserving antigens and process for utilizing cytological material produced by same |
| RU2178565C2 (ru) * | 1996-01-16 | 2002-01-20 | Зила Инк. | Способы и композиции для обнаружения in vivo оральных злокачественных опухолей и предраковых состояний |
| DE10034647C1 (de) * | 2000-07-14 | 2002-04-04 | 3M Espe Ag | Verfahren zur Durchführung einer Speichelanalyse |
| JP2003061511A (ja) * | 2001-08-30 | 2003-03-04 | Sumitomo Pharmaceut Co Ltd | 炎症性疾患治療剤の新規評価方法 |
| FR2854239B1 (fr) * | 2003-04-23 | 2006-03-10 | Reactifs Ral | Procede de preparation d'un reactif de coloration de cellules et reactif obtenu |
| EP1804045B1 (de) * | 2005-12-30 | 2014-03-12 | QIAGEN GmbH | Ein Verfahren und ein Kit zur Behandlung einer biologischen Probe |
| AU2007210068A1 (en) * | 2006-01-26 | 2007-08-09 | The Board Of Regents, The University Of Texas System | Process and apparatus for imaging |
| FR2907220B1 (fr) * | 2006-10-11 | 2009-01-16 | Reactifs Ral Sa | Procede de coloration de cellules |
| EP2027922A1 (de) * | 2007-08-02 | 2009-02-25 | Qiagen GmbH | Verfahren und Vorrichtung für das Fixieren/Stabilisieren einer Probe |
-
2009
- 2009-07-22 FR FR0955127A patent/FR2948456B1/fr active Active
-
2010
- 2010-07-21 HU HUE10752066A patent/HUE028309T2/en unknown
- 2010-07-21 US US13/384,845 patent/US9366604B2/en active Active
- 2010-07-21 RU RU2012106300/05A patent/RU2536502C2/ru active
- 2010-07-21 WO PCT/FR2010/051540 patent/WO2011010064A1/fr not_active Ceased
- 2010-07-21 EP EP10752066.0A patent/EP2457079B1/fr active Active
- 2010-07-21 BR BR112012001495-1A patent/BR112012001495B1/pt not_active IP Right Cessation
- 2010-07-21 IN IN1386DEN2012 patent/IN2012DN01386A/en unknown
- 2010-07-21 ES ES10752066.0T patent/ES2555219T3/es active Active
- 2010-07-21 JP JP2012521086A patent/JP5704466B2/ja active Active
-
2012
- 2012-02-20 ZA ZA2012/01238A patent/ZA201201238B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BR112012001495A2 (pt) | 2020-09-24 |
| US20120122151A1 (en) | 2012-05-17 |
| RU2012106300A (ru) | 2013-08-27 |
| ES2555219T3 (es) | 2015-12-29 |
| WO2011010064A1 (fr) | 2011-01-27 |
| EP2457079B1 (fr) | 2015-09-09 |
| RU2536502C2 (ru) | 2014-12-27 |
| EP2457079A1 (fr) | 2012-05-30 |
| FR2948456B1 (fr) | 2011-09-30 |
| ZA201201238B (en) | 2012-10-31 |
| US9366604B2 (en) | 2016-06-14 |
| BR112012001495B1 (pt) | 2021-07-06 |
| JP2012533745A (ja) | 2012-12-27 |
| JP5704466B2 (ja) | 2015-04-22 |
| IN2012DN01386A (enExample) | 2015-06-05 |
| FR2948456A1 (fr) | 2011-01-28 |
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