HUE025489T2 - Tesztminták multiplexelt analízisei - Google Patents

Tesztminták multiplexelt analízisei Download PDF

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Publication number
HUE025489T2
HUE025489T2 HUE07718147A HUE07718147A HUE025489T2 HU E025489 T2 HUE025489 T2 HU E025489T2 HU E07718147 A HUE07718147 A HU E07718147A HU E07718147 A HUE07718147 A HU E07718147A HU E025489 T2 HUE025489 T2 HU E025489T2
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aptamer
complex
target
molecule
test sample
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HUE07718147A
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English (en)
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James R Heil
Daniel J Schneider
Daniel T Nieuwlandt
Sheri K Wilcox
Dominic Zichi
Todd Gander
Bruce Eaton
Larry Gold
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Somalogic Inc
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/16—Aptamers
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
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    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/313—Irradiation, e.g. UV irradiation
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/205—Aptamer
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

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Claims (6)

  1. TESzimmjAK ^ültiplexelt analízisei Szabadalmi igénypontok
    1, Eljárás egy ; tesztmlníáhan esetlegesen jelen leve célmolekule detektálására, amely eljárás során: (s) énníkeztetönk egy tesztmintát egy apíamerrel, amely specifikus· affinitással: rendelkezik a délmolekula iránt, ahol egy aptarrmr és eélmolekulája közötti kölcsönhatás révén egy aptamaraffinitási komplex képződik, lia a eélmolekula jelen van a tesztmintában, ahol az aiptameralfinltásl komplex egy nem kovalens komplex, amely egy aptamer és eélmolekulája közötti kolesönhatás revén keletkezik; (b) miután az (a) lépésbon létrejön az aptameraünrtásr komplex, a tesztmtntához egy kompéikor molekulát adunk és/vagy kifogjuk az aptamerafftnttási komplexet egy szilárd hordozón, maid olyan oldattal mossuk, amely oldatban jelen varr agy kompetitor molekula, és a kompéikor molekula a következők közül kerül kiválaszlásre; egy ólig on e kleotid, egy pokanlon, egy polidextrán vagy cJNTP-k, és (c) detektáljuk és/vagy kvantllikáljuk az apiamerafhnkás; komplex aptamerét vagy a tesztminta többi részétől elválasztott aptamerafflnltási komplexet, 2.. váz. 1. igénypont szerinti ..eljárás.., ahol a kompetltor egy poiianion........ 3, A 2, Idénypont; szerinti eljárás, ahol a poiianion dextrao-szulfái 4, A 2, igénypont; szerinti eljárás, ahol a poilanioa heparin: vagy polldexínán,
  2. 5, Az 1-4. Igénypontok bármelyike szerinti eljárás,, ahol az aptamer egy egyszáiú nukielnsav vagy egy kétszálo nukleinsav.
  3. 6. Az 5. igénypont szerinti -eljárás, ahol az aptamer DNS vagy RNS.
  4. 7.. Az 1-6. Igénypontok bármelyike szerinti eljárás, ahol a eélmolekula .a következők által alkotott csoportból kerül kiválasztásra: egy fehérje, egy szénhidrát, egy poiiszachand egy gtikopretetn, egy hormon, egy receptor, egy antigén, egy antitest, egy vírus, egy szubszfrát, egy meiaboít. egy kofaktor, egy Inhibitor, egy hatóanyag, egy festék, egy tápanyag, egy növekedési faktor és egy szövet. 8. A 7, Igénypont szerinti eljárás, ahol a célmolekuls egy fehérje, 6, A 8, igénypont szerinti eljárás, ehet a fehérje egy glikoprofelh. 10, A 8, Igénypont szerinti eljárás-, ahol a fehérje egy receptor· vagy egy antitest. 11, A 7. Igénypont szerinti· eljárás, ahol a oélmolekula egy szénhidrát
  5. 12, A H , igénypont szerinti eljárás, ahol szénhidrát egy poilszacharld.
  6. 13, Az 1-12. Igénypontok bármelyike szerinti eljárás, ahol a tesztminta egy biológiai minta, amely a következők által alkotott csoportból kerül kiválasztásra: : teljes vér, leukocllák. perifériás vérből származó mononukleáhs sejtek, plazma, szérum, köpet, kilélegzett levegő, vizelet, ondó, nyál,! menmgeátis folyadék, magzatvíz, rningyíolyadék, nylrokfolyadèk, meIíb;m bó - asp;rátu m ; brooebiálls aspirátum, szrnovláíls folyadék, Izületi asprrátom, sejtek,, egy sepivenai széklet, szövet, egy szövetőlopszfa és cerebrospinalis folyadék, 14, A 18:. Igénypont szerinti eljárás, ahol a: tesztmínta sejtek egy biológia; mintája. 1$, A 14. igénypont szerinti eljárás, ahol a sejtek leukooiíák vagy perifériás vérből származó mononnkleárls sejtek,
HUE07718147A 2006-01-17 2007-01-16 Tesztminták multiplexelt analízisei HUE025489T2 (hu)

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US (1) US20070166740A1 (hu)
EP (3) EP2613147B1 (hu)
JP (3) JP5680827B2 (hu)
KR (1) KR101149688B1 (hu)
CN (1) CN101374965B (hu)
AU (1) AU2007205974A1 (hu)
CA (1) CA2634987C (hu)
DK (1) DK1994171T3 (hu)
ES (3) ES2644499T3 (hu)
HK (2) HK1127093A1 (hu)
HU (1) HUE025489T2 (hu)
PL (1) PL1994171T3 (hu)
PT (1) PT1994171E (hu)
SI (1) SI1994171T1 (hu)
TW (1) TWI507528B (hu)
WO (1) WO2007084886A2 (hu)

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