HU214977B - Eljárás polipeptidek előállítására bakteriális gazdasejtekben javított tulajdonságú, lambda PL-promotort tartalmazó vektorok alkalmazásával - Google Patents

Eljárás polipeptidek előállítására bakteriális gazdasejtekben javított tulajdonságú, lambda PL-promotort tartalmazó vektorok alkalmazásával Download PDF

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Publication number
HU214977B
HU214977B HU853240A HU324085A HU214977B HU 214977 B HU214977 B HU 214977B HU 853240 A HU853240 A HU 853240A HU 324085 A HU324085 A HU 324085A HU 214977 B HU214977 B HU 214977B
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HU
Hungary
Prior art keywords
plasmid
vector
gene
site
dna sequence
Prior art date
Application number
HU853240A
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English (en)
Hungarian (hu)
Other versions
HUT40697A (en
Inventor
Haim Aviv
Daniel Bartfeld
Marian Gorecki
Jacob R. Hartman
Dov Kanner
Avigdor Levanon
Hilla Locker-Giladi
Amos B. Oppernheim
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Bio-Technology General Corp.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/644,105 external-priority patent/US4742004A/en
Application filed by Bio-Technology General Corp. filed Critical Bio-Technology General Corp.
Priority to HU9801343A priority Critical patent/HU215160B/hu
Publication of HUT40697A publication Critical patent/HUT40697A/hu
Publication of HU214977B publication Critical patent/HU214977B/hu

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0089Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/61Growth hormone [GH], i.e. somatotropin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/775Apolipopeptides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • C12N15/69Increasing the copy number of the vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • C12N15/73Expression systems using phage (lambda) regulatory sequences
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
  • Magnetic Resonance Imaging Apparatus (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
HU853240A 1984-08-27 1985-08-27 Eljárás polipeptidek előállítására bakteriális gazdasejtekben javított tulajdonságú, lambda PL-promotort tartalmazó vektorok alkalmazásával HU214977B (hu)

Priority Applications (1)

Application Number Priority Date Filing Date Title
HU9801343A HU215160B (hu) 1984-08-27 1985-08-27 Eljárás humán Cu/Zn szuperoxid dizmutáz előállítására és tisztítására

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US64467184A 1984-08-27 1984-08-27
US64455184A 1984-08-27 1984-08-27
US64511984A 1984-08-27 1984-08-27
US64424584A 1984-08-27 1984-08-27
US06/644,105 US4742004A (en) 1984-08-27 1984-08-27 Method for producing enzymatically active eucaryotic sod in bacteria

Publications (2)

Publication Number Publication Date
HUT40697A HUT40697A (en) 1987-01-28
HU214977B true HU214977B (hu) 1998-08-28

Family

ID=27542038

Family Applications (2)

Application Number Title Priority Date Filing Date
HU853240A HU214977B (hu) 1984-08-27 1985-08-27 Eljárás polipeptidek előállítására bakteriális gazdasejtekben javított tulajdonságú, lambda PL-promotort tartalmazó vektorok alkalmazásával
HU9801343A HU215160B (hu) 1984-08-27 1985-08-27 Eljárás humán Cu/Zn szuperoxid dizmutáz előállítására és tisztítására

Family Applications After (1)

Application Number Title Priority Date Filing Date
HU9801343A HU215160B (hu) 1984-08-27 1985-08-27 Eljárás humán Cu/Zn szuperoxid dizmutáz előállítására és tisztítására

Country Status (15)

Country Link
EP (2) EP0173280B1 (enExample)
JP (4) JP2933280B2 (enExample)
AT (2) ATE79407T1 (enExample)
AU (1) AU603331B2 (enExample)
CA (2) CA1340597C (enExample)
DE (2) DE3588216T2 (enExample)
DK (1) DK384185A (enExample)
GR (1) GR852030B (enExample)
HK (1) HK151695A (enExample)
HU (2) HU214977B (enExample)
IE (1) IE61141B1 (enExample)
IL (3) IL108976A (enExample)
MY (1) MY130321A (enExample)
NZ (1) NZ213154A (enExample)
PT (1) PT81025B (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL71689A0 (en) * 1983-04-29 1984-07-31 Yeda Res & Dev Human superoxide dismutase production
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
ATE69618T1 (de) * 1983-10-03 1991-12-15 Chiron Corp Klonieren von superoxid-dismutase und expression in mikroorganismen.
CA1267615A (en) * 1984-08-27 1990-04-10 Dan Hadary Method for recovering purified growth hormones from genetically engineered microorganisms
JP2594535B2 (ja) * 1984-11-30 1997-03-26 サントリー株式会社 高発現ベクターおよびその利用
US5037806A (en) * 1985-02-22 1991-08-06 Monsanto Company Biologically active method using somatotropins
WO1987002062A1 (en) * 1985-10-04 1987-04-09 Biotechnology Research Partners, Ltd. Recombinant apolipoproteins and methods
GB8701848D0 (en) * 1987-01-28 1987-03-04 Celltech Ltd Polypeptides
IL96136A (en) * 1989-11-07 2000-12-06 Bio Technology General Corp Use of hydrophobic chromatography in a method for purification of copper-zinc superoxide dismutase from cells containing same
WO1991007496A1 (en) * 1989-11-09 1991-05-30 Allied-Signal Inc. Cloning and expression of genes encoding for polypeptides comprised of one or more repeating amino acid sequences
DE4239877C1 (de) * 1992-11-27 1994-03-17 Boehringer Ingelheim Int Stabilisierte Superoxid-Dismutase (SOD)-Zusammensetzung
GB9303988D0 (en) * 1993-02-26 1993-04-14 Health Lab Service Board Bi-functional expression systems
US6030810A (en) * 1997-02-26 2000-02-29 Delgado; Stephen Gregory Cloned tetrodotoxin-sensitive sodium channel α-subunit and a splice variant thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK143412C (da) * 1979-05-17 1982-05-24 Forenede Bryggerier As Fremgangsmaade til udvinding af cu,zn-superoxid-dismutase fra gaer
KR860001557B1 (ko) * 1980-06-06 1986-10-04 비오겐 엔·브이 폴리펩티드의 제조방법
US4506013A (en) * 1980-10-03 1985-03-19 Eli Lilly And Company Stabilizing and selecting recombinant DNA host cells
CA1341012C (en) * 1982-09-27 2000-06-06 Biogen, Inc. Dna sequences, recombinant dna molecules and processes for producing swine growth hormone-like polypeptides
CA1303530C (en) * 1983-01-21 1992-06-16 Michael Courtney EXPRESSION VECTORS AND THEIR USE FOR THE PREPARATION OF A PROTEIN HAVING HUMAN .alpha. -ANTITRYPSIN ACTIVITY
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
DE3486493T2 (de) * 1983-10-03 2003-05-22 Chiron Corp. (N.D.Ges.D. Staates Delaware), Emeryville Klonieren von Superoxiddismutase und Expression in Mikroorganismen

Also Published As

Publication number Publication date
EP0173280B1 (en) 1992-08-12
CA1340464C (en) 1999-03-23
IL76192A (en) 1996-12-05
DE3586473D1 (de) 1992-09-17
HU215160B (hu) 1998-10-28
ATE79407T1 (de) 1992-08-15
HUT40697A (en) 1987-01-28
JP2933280B2 (ja) 1999-08-09
PT81025A (en) 1985-09-01
EP0483113A2 (en) 1992-04-29
JP2688180B2 (ja) 1997-12-08
IL76192A0 (en) 1985-12-31
AU603331B2 (en) 1990-11-15
DK384185D0 (da) 1985-08-23
MY130321A (en) 2007-06-29
IL108976A (en) 1996-12-05
JPS61111693A (ja) 1986-05-29
AU4667785A (en) 1986-03-06
IL110039A (en) 1996-12-05
DE3586473T2 (de) 1993-02-04
JPH07222596A (ja) 1995-08-22
ATE184916T1 (de) 1999-10-15
EP0173280A1 (en) 1986-03-05
PT81025B (pt) 1988-01-22
IL108976A0 (en) 1994-06-24
JP2777074B2 (ja) 1998-07-16
NZ213154A (en) 1988-11-29
IE852093L (en) 1986-02-27
CA1340597C (en) 1999-06-22
EP0483113B1 (en) 1999-09-22
DE3588216T2 (de) 2000-02-03
IL110039A0 (en) 1994-10-07
IE61141B1 (en) 1994-10-05
GR852030B (enExample) 1985-12-30
HK151695A (en) 1995-09-29
JPH07236490A (ja) 1995-09-12
DE3588216D1 (de) 1999-10-28
EP0483113A3 (en) 1992-05-13
JPH08283296A (ja) 1996-10-29
DK384185A (da) 1986-02-28

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