MY130321A - Expression vector containing pl promoter and engineered restriction site for convenient replacement of ribosomal binding site, plasmid containing the vectors, hosts containing the plasmids and relatedmethods - Google Patents

Expression vector containing pl promoter and engineered restriction site for convenient replacement of ribosomal binding site, plasmid containing the vectors, hosts containing the plasmids and relatedmethods

Info

Publication number
MY130321A
MY130321A MYPI87002785A MYPI8702785A MY130321A MY 130321 A MY130321 A MY 130321A MY PI87002785 A MYPI87002785 A MY PI87002785A MY PI8702785 A MYPI8702785 A MY PI8702785A MY 130321 A MY130321 A MY 130321A
Authority
MY
Malaysia
Prior art keywords
site
vectors
host
vector
promoter
Prior art date
Application number
MYPI87002785A
Other languages
English (en)
Inventor
Haim Aviv
Daniel Bartfeld
Marian Gorecki
Jacob R Hartman
Dov Kanner
Avigdor Levanon
Hilla Locker-Giladi
Amos B Oppenheim
Original Assignee
Bio Technology General Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/644,105 external-priority patent/US4742004A/en
Application filed by Bio Technology General Corp filed Critical Bio Technology General Corp
Publication of MY130321A publication Critical patent/MY130321A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/775—Apolipopeptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
  • Magnetic Resonance Imaging Apparatus (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
MYPI87002785A 1984-08-27 1987-10-01 Expression vector containing pl promoter and engineered restriction site for convenient replacement of ribosomal binding site, plasmid containing the vectors, hosts containing the plasmids and relatedmethods MY130321A (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US64424584A 1984-08-27 1984-08-27
US64467184A 1984-08-27 1984-08-27
US64455184A 1984-08-27 1984-08-27
US64511984A 1984-08-27 1984-08-27
US06/644,105 US4742004A (en) 1984-08-27 1984-08-27 Method for producing enzymatically active eucaryotic sod in bacteria
EP85110683A EP0173280B1 (en) 1984-08-27 1985-08-26 Plasmids containing lambda pl promoter, and engineered restriction site for convenient replacement of ribosomal binding site, hosts containing the plasmids and related methods

Publications (1)

Publication Number Publication Date
MY130321A true MY130321A (en) 2007-06-29

Family

ID=27542038

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI87002785A MY130321A (en) 1984-08-27 1987-10-01 Expression vector containing pl promoter and engineered restriction site for convenient replacement of ribosomal binding site, plasmid containing the vectors, hosts containing the plasmids and relatedmethods

Country Status (15)

Country Link
EP (2) EP0173280B1 (enExample)
JP (4) JP2933280B2 (enExample)
AT (2) ATE79407T1 (enExample)
AU (1) AU603331B2 (enExample)
CA (2) CA1340597C (enExample)
DE (2) DE3588216T2 (enExample)
DK (1) DK384185A (enExample)
GR (1) GR852030B (enExample)
HK (1) HK151695A (enExample)
HU (2) HU215160B (enExample)
IE (1) IE61141B1 (enExample)
IL (3) IL76192A (enExample)
MY (1) MY130321A (enExample)
NZ (1) NZ213154A (enExample)
PT (1) PT81025B (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL71689A0 (en) * 1983-04-29 1984-07-31 Yeda Res & Dev Human superoxide dismutase production
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
ATE69618T1 (de) * 1983-10-03 1991-12-15 Chiron Corp Klonieren von superoxid-dismutase und expression in mikroorganismen.
CA1267615A (en) * 1984-08-27 1990-04-10 Dan Hadary Method for recovering purified growth hormones from genetically engineered microorganisms
JP2594535B2 (ja) * 1984-11-30 1997-03-26 サントリー株式会社 高発現ベクターおよびその利用
US5037806A (en) * 1985-02-22 1991-08-06 Monsanto Company Biologically active method using somatotropins
EP0239631A4 (en) * 1985-10-04 1989-01-12 Biotech Res Partners Ltd RECOMBINANT APOLIPOPROTEINS AND METHODS.
GB8701848D0 (en) * 1987-01-28 1987-03-04 Celltech Ltd Polypeptides
IL96136A (en) * 1989-11-07 2000-12-06 Bio Technology General Corp Use of hydrophobic chromatography in a method for purification of copper-zinc superoxide dismutase from cells containing same
WO1991007496A1 (en) * 1989-11-09 1991-05-30 Allied-Signal Inc. Cloning and expression of genes encoding for polypeptides comprised of one or more repeating amino acid sequences
DE4239877C1 (de) * 1992-11-27 1994-03-17 Boehringer Ingelheim Int Stabilisierte Superoxid-Dismutase (SOD)-Zusammensetzung
GB9303988D0 (en) * 1993-02-26 1993-04-14 Health Lab Service Board Bi-functional expression systems
US6030810A (en) 1997-02-26 2000-02-29 Delgado; Stephen Gregory Cloned tetrodotoxin-sensitive sodium channel α-subunit and a splice variant thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK143412C (da) * 1979-05-17 1982-05-24 Forenede Bryggerier As Fremgangsmaade til udvinding af cu,zn-superoxid-dismutase fra gaer
FI811009A7 (fi) * 1980-06-06 1981-12-07 Biogen Nv Parannettuja vektoreita, näiden vektoreiden valmistusmenetelmiä ja kloonattujen geenien ekspressiomeneekspressiomenetelmiä.
US4506013A (en) * 1980-10-03 1985-03-19 Eli Lilly And Company Stabilizing and selecting recombinant DNA host cells
CA1341012C (en) * 1982-09-27 2000-06-06 Biogen, Inc. Dna sequences, recombinant dna molecules and processes for producing swine growth hormone-like polypeptides
DE3482840D1 (de) * 1983-01-21 1990-09-06 Transgene Sa Expressionsvektoren und deren verwendung zur herstellung eines proteins mit menschlicher alpha-antitrypsin-aktivitaet.
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
DE3486452T2 (de) * 1983-10-03 1997-11-06 Chiron Corp., Emeryville, Calif. Superoxiddismutase und deren Expression in Mikroorganismen

Also Published As

Publication number Publication date
IL108976A0 (en) 1994-06-24
AU4667785A (en) 1986-03-06
IL76192A (en) 1996-12-05
IL108976A (en) 1996-12-05
JPH07222596A (ja) 1995-08-22
JP2777074B2 (ja) 1998-07-16
PT81025B (pt) 1988-01-22
EP0483113A3 (en) 1992-05-13
ATE79407T1 (de) 1992-08-15
IE61141B1 (en) 1994-10-05
DE3586473T2 (de) 1993-02-04
JP2688180B2 (ja) 1997-12-08
AU603331B2 (en) 1990-11-15
GR852030B (enExample) 1985-12-30
EP0483113B1 (en) 1999-09-22
HK151695A (en) 1995-09-29
DE3586473D1 (de) 1992-09-17
IL110039A (en) 1996-12-05
NZ213154A (en) 1988-11-29
EP0173280A1 (en) 1986-03-05
IL76192A0 (en) 1985-12-31
DE3588216D1 (de) 1999-10-28
EP0483113A2 (en) 1992-04-29
JPS61111693A (ja) 1986-05-29
DK384185D0 (da) 1985-08-23
JPH08283296A (ja) 1996-10-29
JPH07236490A (ja) 1995-09-12
HU215160B (hu) 1998-10-28
JP2933280B2 (ja) 1999-08-09
ATE184916T1 (de) 1999-10-15
EP0173280B1 (en) 1992-08-12
DE3588216T2 (de) 2000-02-03
CA1340464C (en) 1999-03-23
DK384185A (da) 1986-02-28
CA1340597C (en) 1999-06-22
HUT40697A (en) 1987-01-28
PT81025A (en) 1985-09-01
IE852093L (en) 1986-02-27
HU214977B (hu) 1998-08-28
IL110039A0 (en) 1994-10-07

Similar Documents

Publication Publication Date Title
DK173085B1 (da) Fremgangsmåde til bakteriel polypeptidudtrykkelse og plasmidisk udtrykkelsesmedium til fremstilling af et heterologt polype
MY130321A (en) Expression vector containing pl promoter and engineered restriction site for convenient replacement of ribosomal binding site, plasmid containing the vectors, hosts containing the plasmids and relatedmethods
ES2272303T3 (es) Procedimientos de produccion de l-aminoacidos mediante el incremento de nadph celular.
ES8602947A1 (es) Un metodo para producir un polipeptido.
GB2137208B (en) The use of the gal1 promoter
US4742004A (en) Method for producing enzymatically active eucaryotic sod in bacteria
Zhang et al. Cloning, sequencing, expression, and regulation of the structural gene for the copper/topa quinone-containing methylamine oxidase from Arthrobacter strain P1, a gram-positive facultative methylotroph
IL123641A0 (en) Circular dna molecule with conditional origin of replication method for preparing the same and use thereof in gene therapy
EP0643135A4 (en) PROCESS FOR PRODUCING L-THREONINE BY FERMENTATION.
Amichay et al. Construction of a Synechocystic PCC6803 mutant suitable for the study of variant hexadecameric ribulose bisphosphate carboxylase/oxygenase enzymes
EP0528686A2 (en) Process for producing peptides
NZ334568A (en) Nucleic acid and amino acid sequences relating to helicobacter pylori and vaccine compositions thereof
US4663294A (en) DNA coding for a signal amino acid sequence and DNA containing the same
Stuitje et al. The nucleotide sequence surrounding the replication origin of the cop3 mutant of the bacterio-cinogenic plasmid Clo DF13
DE3880520T2 (de) Rekombinantes plasmid fuer die expression von l-phenylalanin-ammonialyase und transformierte staemme, die es enthalten.
KR910001046A (ko) 비융합 단백질을 제조하기 위한 미생물내 발현용 발현벡터
EP0164245A3 (en) Nif promoter of rhizobium japonicum
ES2003085A6 (es) Procedimiento para inducir de una forma controlada la expresion de genes eucarioticos de interes en celulas hospedantes eucarioticas apropiadas
US5593860A (en) Expression plasmids containing the deo promoter, and bacterial hosts containing the plasmids
US5126252A (en) Expressions vectors containing Lambda PL promoter and T1 T2 RNA termination sequence, plasmids containing the vectors, hosts containing the plasmids and relatedmethods
JP3325597B2 (ja) ポリペプチドの製法
US5147789A (en) Stabilized expression vectors containing lambda pl promoter and the gene for the CI434 repressor, plasmids containing the vectors, hosts containing the plasmids and related methods
WO1993008277A1 (en) Expression of polypeptides in e.coli under control of the e.coli mdh-gene promoter
Umeno et al. Properties of the RecB and RecC gene products of EscherichiaColi
EP0352763A1 (en) DNA fragment containing gene which encodes the function of stabilizing plasmid in host microorganism