HRP970077A2 - Thrombin muteins as antidotes for thrombin inhibitors - Google Patents
Thrombin muteins as antidotes for thrombin inhibitors Download PDFInfo
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- HRP970077A2 HRP970077A2 HR19605126.6A HRP970077A HRP970077A2 HR P970077 A2 HRP970077 A2 HR P970077A2 HR P970077 A HRP970077 A HR P970077A HR P970077 A2 HRP970077 A2 HR P970077A2
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- thrombin
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- prothrombin
- sequence
- mutant
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- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 229940105776 factor viii inhibitor bypassing activity Drugs 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000002008 hemorrhagic effect Effects 0.000 description 1
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 1
- 125000004356 hydroxy functional group Chemical group O* 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229960004927 neomycin Drugs 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 210000003516 pericardium Anatomy 0.000 description 1
- 210000004303 peritoneum Anatomy 0.000 description 1
- 230000036470 plasma concentration Effects 0.000 description 1
- 210000004224 pleura Anatomy 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 108010071286 prethrombins Proteins 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 229950008679 protamine sulfate Drugs 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 230000008521 reorganization Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000003118 sandwich ELISA Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 230000010473 stable expression Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000003869 thrombin derivative Substances 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000010474 transient expression Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 125000002987 valine group Chemical group [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6429—Thrombin (3.4.21.5)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21005—Thrombin (3.4.21.5)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19605126A DE19605126A1 (de) | 1996-02-12 | 1996-02-12 | Thrombinmuteine als Antidot für Thrombininhibitoren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP970077A2 true HRP970077A2 (en) | 1998-04-30 |
Family
ID=7785198
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HR19605126.6A HRP970077A2 (en) | 1996-02-12 | 1997-02-11 | Thrombin muteins as antidotes for thrombin inhibitors |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US6060300A (es) |
| EP (1) | EP0880591A1 (es) |
| JP (1) | JPH11512621A (es) |
| KR (1) | KR19990082479A (es) |
| CN (1) | CN1211280A (es) |
| AR (1) | AR005797A1 (es) |
| AU (1) | AU1768497A (es) |
| BR (1) | BR9707280A (es) |
| CA (1) | CA2245546A1 (es) |
| CO (1) | CO4600682A1 (es) |
| CZ (1) | CZ247798A3 (es) |
| DE (1) | DE19605126A1 (es) |
| HR (1) | HRP970077A2 (es) |
| HU (1) | HUP9900574A2 (es) |
| IL (1) | IL125077A0 (es) |
| NO (1) | NO983677L (es) |
| WO (1) | WO1997029198A1 (es) |
| ZA (1) | ZA971107B (es) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20000018933A (ko) * | 1998-09-07 | 2000-04-06 | 김승수 | 내피세포 성장억제 활성을 가지는 사람 프로트롬빈 크링글단백질 및 이를 암호하는 유전자 |
| GB2355161B (en) * | 1999-10-05 | 2001-09-12 | 3Com Corp | Network device incorporating selective compression of stored data packets |
| KR20020008471A (ko) * | 2000-07-20 | 2002-01-31 | 김승수 | 내피세포 성장 억제활성을 가지는 인간 프로트롬빈크링글, 그것의 재조합벡터 및 이를 발현하는 재조합 숙주 |
| CA2644926A1 (en) * | 2006-03-06 | 2007-09-13 | Humagene, Inc. | A method for the preparation of recombinant human thrombin and fibrinogen |
| BRPI0816837B1 (pt) | 2007-09-28 | 2022-10-18 | Portola Pharmaceuticals, Inc | Composições farmacêuticas, polipeptídeo isolado de duas cadeias e uso de uma composição farmacêutica |
| WO2010056765A2 (en) | 2008-11-14 | 2010-05-20 | Portola Pharmaceuticals, Inc. | Antidotes for factor xa inhibitors and methods of using the same in combination with blood coagulating agents |
| ES2607935T3 (es) | 2009-03-30 | 2017-04-04 | Portola Pharmaceuticals, Inc. | Antídotos para inhibidores del factor Xa y procedimientos de uso de los mismos |
| CA2767858C (en) * | 2009-07-15 | 2019-02-12 | Portola Pharmaceuticals, Inc. | Unit dose formulation of antidotes for factor xa inhibitors and methods of using the same |
| ES2620421T3 (es) | 2009-10-30 | 2017-06-28 | Senova Gesellschaft für Biowissenschaft und Technik mbH | Peptidasas acopladas a polímero |
| AR079944A1 (es) | 2010-01-20 | 2012-02-29 | Boehringer Ingelheim Int | Anticuerpo neutralizante de la actividad de un anticoagulante |
| EP2691156A1 (en) | 2011-03-30 | 2014-02-05 | Boehringer Ingelheim International GmbH | Anticoagulant antidotes |
| JP6640198B2 (ja) | 2014-05-26 | 2020-02-05 | アカデ−ミッシュ ズィーケンハウス ライデンＡｃａｄｅｍｉｓｃｈ Ｚｉｅｋｅｎｈｕｉｓ Ｌｅｉｄｅｎ | 出血治療のための止血促進タンパク質 |
| EP3971576A1 (en) | 2020-09-21 | 2022-03-23 | Instrumentation Laboratory Company | Detecting and monitoring oral anticoagulants or intravenous direct thrombin inhibitors in a blood sample |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4203965A1 (de) * | 1992-02-11 | 1993-08-12 | Max Planck Gesellschaft | Antidot fuer hirudin und synthetische thrombininhibitoren |
-
1996
- 1996-02-12 DE DE19605126A patent/DE19605126A1/de not_active Withdrawn
-
1997
- 1997-02-11 AU AU17684/97A patent/AU1768497A/en not_active Abandoned
- 1997-02-11 CA CA002245546A patent/CA2245546A1/en not_active Abandoned
- 1997-02-11 CO CO97006960A patent/CO4600682A1/es unknown
- 1997-02-11 ZA ZA971107A patent/ZA971107B/xx unknown
- 1997-02-11 IL IL12507797A patent/IL125077A0/xx unknown
- 1997-02-11 EP EP97903254A patent/EP0880591A1/de not_active Withdrawn
- 1997-02-11 HR HR19605126.6A patent/HRP970077A2/hr not_active Application Discontinuation
- 1997-02-11 JP JP9528173A patent/JPH11512621A/ja active Pending
- 1997-02-11 CZ CZ982477A patent/CZ247798A3/cs unknown
- 1997-02-11 KR KR1019980706212A patent/KR19990082479A/ko not_active Application Discontinuation
- 1997-02-11 BR BR9707280A patent/BR9707280A/pt not_active Application Discontinuation
- 1997-02-11 WO PCT/EP1997/000612 patent/WO1997029198A1/de not_active Application Discontinuation
- 1997-02-11 CN CN97192210A patent/CN1211280A/zh active Pending
- 1997-02-11 HU HU9900574A patent/HUP9900574A2/hu unknown
- 1997-02-11 US US09/117,708 patent/US6060300A/en not_active Expired - Fee Related
- 1997-02-12 AR ARP970100548A patent/AR005797A1/es unknown
-
1998
- 1998-08-11 NO NO983677A patent/NO983677L/no unknown
Also Published As
| Publication number | Publication date |
|---|---|
| HUP9900574A2 (hu) | 1999-06-28 |
| EP0880591A1 (de) | 1998-12-02 |
| CN1211280A (zh) | 1999-03-17 |
| NO983677D0 (no) | 1998-08-11 |
| CZ247798A3 (cs) | 1999-03-17 |
| IL125077A0 (en) | 1999-01-26 |
| JPH11512621A (ja) | 1999-11-02 |
| ZA971107B (en) | 1998-08-11 |
| NO983677L (no) | 1998-08-11 |
| WO1997029198A1 (de) | 1997-08-14 |
| CO4600682A1 (es) | 1998-05-08 |
| KR19990082479A (ko) | 1999-11-25 |
| AR005797A1 (es) | 1999-07-14 |
| CA2245546A1 (en) | 1997-08-14 |
| DE19605126A1 (de) | 1997-08-14 |
| AU1768497A (en) | 1997-08-28 |
| BR9707280A (pt) | 1999-07-20 |
| US6060300A (en) | 2000-05-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A1OB | Publication of a patent application | ||
| ODBC | Application rejected |