HRP20191573T1 - Postupak za enzimsku pripremu proizvoda glukozida i nusproizvoda iz edukta glukozida - Google Patents
Postupak za enzimsku pripremu proizvoda glukozida i nusproizvoda iz edukta glukozida Download PDFInfo
- Publication number
- HRP20191573T1 HRP20191573T1 HRP20191573T HRP20191573T1 HR P20191573 T1 HRP20191573 T1 HR P20191573T1 HR P20191573 T HRP20191573 T HR P20191573T HR P20191573 T1 HRP20191573 T1 HR P20191573T1
- Authority
- HR
- Croatia
- Prior art keywords
- phosphorylase
- phosphate
- sucrose
- cellobiose
- preparation
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims 17
- 238000002360 preparation method Methods 0.000 title claims 12
- 230000002255 enzymatic effect Effects 0.000 title claims 2
- 229930182478 glucoside Natural products 0.000 title 2
- 150000008131 glucosides Chemical class 0.000 title 2
- 108020000005 Sucrose phosphorylase Proteins 0.000 claims 9
- 108010048610 cellobiose phosphorylase Proteins 0.000 claims 9
- 229910019142 PO4 Inorganic materials 0.000 claims 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 7
- 239000010452 phosphate Substances 0.000 claims 7
- HXXFSFRBOHSIMQ-VFUOTHLCSA-N alpha-D-glucose 1-phosphate Chemical compound OC[C@H]1O[C@H](OP(O)(O)=O)[C@H](O)[C@@H](O)[C@@H]1O HXXFSFRBOHSIMQ-VFUOTHLCSA-N 0.000 claims 6
- 229950010772 glucose-1-phosphate Drugs 0.000 claims 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 5
- 229930006000 Sucrose Natural products 0.000 claims 5
- 239000005720 sucrose Substances 0.000 claims 5
- 241001156739 Actinobacteria <phylum> Species 0.000 claims 4
- 241000894006 Bacteria Species 0.000 claims 4
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 claims 3
- 239000012528 membrane Substances 0.000 claims 3
- 229930091371 Fructose Natural products 0.000 claims 2
- 239000005715 Fructose Substances 0.000 claims 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 238000000909 electrodialysis Methods 0.000 claims 2
- 239000008103 glucose Substances 0.000 claims 2
- 238000006555 catalytic reaction Methods 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 238000002425 crystallisation Methods 0.000 claims 1
- 230000008025 crystallization Effects 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/0102—Cellobiose phosphorylase (2.4.1.20)
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/02—Glucose; Glucose-containing syrups obtained by saccharification of cellulosic materials
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K11/00—Fructose
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Emergency Medicine (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (15)
1. Enzimski postupak za pripremu celobioze i fruktoze iz saharoze, naznačen time da postupak sadrži korake:
(a) dobivanje prvog pripravka edukta koji sadrži saharozu fosforilazu, saharozu i fosfat;
(b) reagiranje barem dijela saharoze sadržane u prvom pripravku edukta sa najmanje dijelom fosfata sadržanog u prvom pripravku edukta s katalizom pomoću saharoze fosforilaze, pri čemu je dobiven prvi pripravak proizvoda koji sadrži fruktozu i glukozu 1-fosfat;
(c) dobivanje drugog pripravka edukta koji sadrži celobiozu fosforilazu, glukozu i najmanje dio glukoze 1-fosfat dobivene u koraku (b); i
(d) reakciju glukoze s barem dijelom glukoze 1-fosfata sadržanog u drugom pripravku edukta s katalizom pomoću celobioze fosforilaze, gdje je dobiven drugi pripravak proizvoda koji sadrži celobiozu i fosfat;
pri čemu se koraci (b) i (d) izvode u zajedničkom reaktoru; a gdje saharoza fosforilaza i celobioza fosforilaza nisu imobilizirane na čvrstom nosaču, niti su zatvorene u matricu.
2. Postupak prema zahtjevu 1, naznačen time da se saharoza fosforilaza, i/ili celobioza fosforilaza recikliraju.
3. Postupak prema zahtjevu 1 ili 2, naznačen time da se provodi u jednom membranskom reaktoru.
4. Postupak prema zahtjevu 3, naznačen time da saharoza fosforilaza, i/ili celobioza fosforilaza ne mogu proći membranu membranskog reaktora.
5. Postupak prema bili kojem od prethodnih zahtjeva, naznačen time da se koraci (b) i (d) izvode barem djelomično istovremeno.
6. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time da saharoza fosforilaza je saharoza fosforilaza divljeg tipa bakterije koja pripada rodu Actinobacteria, ili saharoza fosforilaza koja se razlikuje od navedenog divljeg tipa, ali koja sadrži sekvencu aminokiseline koja ima najmanje 80% identiteta u odnosu na saharozu fosforilazu divljeg tipa bakterije koja pripada rodu Actinobacteria.
7. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time da celobioza fosforilaza je celobioza fosforilaza divljeg tipa bakterije koja pripada rodu Actinobacteria, ili celobioza fosforilaza koja se razlikuje od navedenog divljeg tipa, ali koja sadrži sekvencu aminokiseline koja ima najmanje 80% identiteta u odnosu na celobiozu fosforilazu divljeg tipa bakterije koja pripada rodu Actinobacteria.
8. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time da je koncentracija saharoze u prvom pripravku edukta danog u koraku (a) najmanje 0.150 mol/l; po mogućnosti najmanje 0.200 mol/l; poželjnije najmanje 0.500 mol/l.
9. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time da je koncentracija celobioze u drugom pripravku proizvoda dobivenog u koraku (d) najmanje 0.075 mol/l, po mogućnosti najmanje 0.150 mol/l; poželjnije najmanje 0.200 mol/l.
10. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time da korak (b) dodatno sadrži pomoćni korak (b') koji odvaja barem dio rezultirajuće glukoze 1-fosfat tijekom reakcije saharoze u glukozu 1-fosfat.
11. Postupak prema zahtjevu 10, naznačen time da se u koraku (b') glukoza 1-fosfat odvaja elektrodijalizom i/ili kristalizacijom.
12. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time da sadrži dodatni korak: (g) odvajanje fosfata dobivenog u koraku (d) i opcionalno vraćanje u korak (a).
13. Postupak prema bilo kojem od zahtjeva 12, naznačen time da je fosfat izdvojen pomoću elektrodijalize.
14. Postupak prema zahtjevu 12 ili 13, naznačen time da je najmanje 50% fosfata koji je izdvojen u koraku (g) vraćeno u korak (a) kao barem dio fosfata koji je sadržan u prvom pripravku edukta.
15. Postupak prema bilo kojem od zahtjeva 12 d o14, naznačen time da se korak (g) izvodi vremenski nakon koraka (d).
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14184301 | 2014-09-10 | ||
| EP14184302 | 2014-09-10 | ||
| EP14190891 | 2014-10-29 | ||
| PCT/EP2015/070724 WO2016038142A1 (en) | 2014-09-10 | 2015-09-10 | Process for the enzymatic preparation of a product glucoside and of a co-product from an educt glucoside |
| EP15760467.9A EP3191598B1 (en) | 2014-09-10 | 2015-09-10 | Process for the enzymatic preparation of a product glucoside and of a co-product from an educt glucoside |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20191573T1 true HRP20191573T1 (hr) | 2019-11-29 |
Family
ID=54065891
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20191573 HRP20191573T1 (hr) | 2014-09-10 | 2019-08-30 | Postupak za enzimsku pripremu proizvoda glukozida i nusproizvoda iz edukta glukozida |
| HRP20200170TT HRP20200170T1 (hr) | 2014-09-10 | 2020-02-03 | Celobioza fosforilaza |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20200170TT HRP20200170T1 (hr) | 2014-09-10 | 2020-02-03 | Celobioza fosforilaza |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US10494616B2 (hr) |
| EP (2) | EP3191586B1 (hr) |
| DK (2) | DK3191586T3 (hr) |
| ES (1) | ES2745449T3 (hr) |
| HR (2) | HRP20191573T1 (hr) |
| HU (2) | HUE047736T2 (hr) |
| PL (2) | PL3191586T3 (hr) |
| PT (1) | PT3191598T (hr) |
| SI (2) | SI3191586T1 (hr) |
| WO (2) | WO2016038141A1 (hr) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3237631A1 (en) * | 2014-12-23 | 2017-11-01 | E. I. du Pont de Nemours and Company | Enzymatically produced cellulose |
| EP3538239A1 (en) | 2016-11-11 | 2019-09-18 | Pfeifer & Langen GmbH & Co. KG | Synthesis of d-allulose |
| CN108611386A (zh) * | 2016-12-12 | 2018-10-02 | 中国科学院天津工业生物技术研究所 | 多酶催化制备纤维二糖的方法 |
| WO2019166514A1 (en) | 2018-02-28 | 2019-09-06 | C-Lecta Gmbh | Enzymatic in-situ fortification of food with functional carbohydrates |
| BR112021000097A2 (pt) * | 2018-07-05 | 2021-03-30 | Dupont Nutrition Biosciences Aps | Uso de glucosil transferase para fornecer melhor textura em produtos à base de leite fermentado |
| CN115851648A (zh) * | 2021-09-24 | 2023-03-28 | 中国科学院天津工业生物技术研究所 | 一种热稳定性及催化活性提高的甘油葡萄糖苷磷酸化酶突变体及其应用 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62159803A (ja) | 1985-12-26 | 1987-07-15 | レツクスノ−ド・インコ−ポレ−テツド | 植込締付具組立体の固締リング |
| JPH01256394A (ja) | 1988-04-06 | 1989-10-12 | Natl Food Res Inst | セロオリゴ糖の酵素的製造方法 |
| JP2815023B2 (ja) | 1989-10-17 | 1998-10-27 | 農林水産省食品総合研究所長 | セロビオースの製造方法 |
| CA2038485A1 (en) | 1990-03-23 | 1991-09-24 | Donald K. Hadden | Nanofiltration process for making dextrose |
| JP2995289B2 (ja) * | 1997-08-04 | 1999-12-27 | 農林水産省食品総合研究所長 | セロビオースフォスフォリラーゼ遺伝子、該遺伝子を含むベクター及び形質転換体 |
| US6355110B1 (en) | 1999-11-17 | 2002-03-12 | Tate & Lyle Industries, Limited | Process for purification of low grade sugar syrups using nanofiltration |
| JP4703950B2 (ja) | 2003-07-23 | 2011-06-15 | 花王株式会社 | 糖リン酸エステルの精製法 |
| WO2005056811A1 (ja) * | 2003-12-12 | 2005-06-23 | Ezaki Glico Co., Ltd. | β−1，4−グルカンをα−グルカンに変換する方法 |
| JP4898135B2 (ja) | 2005-04-13 | 2012-03-14 | 松谷化学工業株式会社 | セロビオースの精製方法及び製造方法 |
| FI120590B (fi) | 2005-10-28 | 2009-12-15 | Danisco Sweeteners Oy | Erotusmenetelmä |
| JP2011505862A (ja) * | 2007-12-20 | 2011-03-03 | ウニベルズィタイト・ヘント | 新規ラクトースホスホリラーゼ酵素 |
| CN101376901A (zh) | 2008-09-27 | 2009-03-04 | 江南大学 | 一种高纯度低聚果糖的制备方法 |
| JP4448548B2 (ja) | 2008-12-22 | 2010-04-14 | 花王株式会社 | グルコース−１−リン酸の製造法 |
| EP2402454A1 (en) | 2010-06-30 | 2012-01-04 | Süd-Chemie AG | Cellobiose production from biomass |
| CN103597084A (zh) * | 2011-02-07 | 2014-02-19 | 加利福尼亚大学董事会 | 增强的纤维糊精代谢 |
| EP2909318A1 (en) | 2012-10-18 | 2015-08-26 | Universiteit Gent | A thermostable sucrose and sucrose-6'-phosphate phosphorylase |
-
2015
- 2015-09-10 PL PL15763296T patent/PL3191586T3/pl unknown
- 2015-09-10 WO PCT/EP2015/070722 patent/WO2016038141A1/de active Application Filing
- 2015-09-10 SI SI201531063T patent/SI3191586T1/sl unknown
- 2015-09-10 ES ES15760467T patent/ES2745449T3/es active Active
- 2015-09-10 EP EP15763296.9A patent/EP3191586B1/de active Active
- 2015-09-10 PL PL15760467T patent/PL3191598T3/pl unknown
- 2015-09-10 SI SI201530862T patent/SI3191598T1/sl unknown
- 2015-09-10 DK DK15763296.9T patent/DK3191586T3/da active
- 2015-09-10 EP EP15760467.9A patent/EP3191598B1/en active Active
- 2015-09-10 US US15/506,215 patent/US10494616B2/en active Active
- 2015-09-10 WO PCT/EP2015/070724 patent/WO2016038142A1/en active Application Filing
- 2015-09-10 HU HUE15763296A patent/HUE047736T2/hu unknown
- 2015-09-10 PT PT15760467T patent/PT3191598T/pt unknown
- 2015-09-10 DK DK15760467.9T patent/DK3191598T3/da active
- 2015-09-10 HU HUE15760467A patent/HUE046015T2/hu unknown
-
2019
- 2019-08-30 HR HRP20191573 patent/HRP20191573T1/hr unknown
-
2020
- 2020-02-03 HR HRP20200170TT patent/HRP20200170T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL3191598T3 (pl) | 2019-12-31 |
| US10494616B2 (en) | 2019-12-03 |
| EP3191586A1 (de) | 2017-07-19 |
| WO2016038142A1 (en) | 2016-03-17 |
| PL3191586T3 (pl) | 2020-05-18 |
| SI3191586T1 (sl) | 2020-03-31 |
| DK3191598T3 (da) | 2019-09-23 |
| HUE047736T2 (hu) | 2020-05-28 |
| PT3191598T (pt) | 2019-09-12 |
| ES2745449T3 (es) | 2020-03-02 |
| EP3191598A1 (en) | 2017-07-19 |
| HRP20200170T1 (hr) | 2020-05-15 |
| DK3191586T3 (en) | 2020-02-10 |
| WO2016038141A1 (de) | 2016-03-17 |
| US20180216086A1 (en) | 2018-08-02 |
| SI3191598T1 (sl) | 2019-10-30 |
| EP3191586B1 (de) | 2019-11-13 |
| HUE046015T2 (hu) | 2020-02-28 |
| EP3191598B1 (en) | 2019-06-19 |
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