HRP20161224T1 - Poboljšani promotor, i postupak proizvodnje l-lizina uporabom istog - Google Patents
Poboljšani promotor, i postupak proizvodnje l-lizina uporabom istog Download PDFInfo
- Publication number
- HRP20161224T1 HRP20161224T1 HRP20161224TT HRP20161224T HRP20161224T1 HR P20161224 T1 HRP20161224 T1 HR P20161224T1 HR P20161224T T HRP20161224T T HR P20161224TT HR P20161224 T HRP20161224 T HR P20161224T HR P20161224 T1 HRP20161224 T1 HR P20161224T1
- Authority
- HR
- Croatia
- Prior art keywords
- transformant
- lysine
- nucleic acid
- acid molecule
- same
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title claims 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims 2
- 239000004472 Lysine Substances 0.000 claims 2
- GMKMEZVLHJARHF-UHFFFAOYSA-N 2,6-diaminopimelic acid Chemical compound OC(=O)C(N)CCCC(N)C(O)=O GMKMEZVLHJARHF-UHFFFAOYSA-N 0.000 claims 1
- 241000186146 Brevibacterium Species 0.000 claims 1
- 241000186216 Corynebacterium Species 0.000 claims 1
- 230000006801 homologous recombination Effects 0.000 claims 1
- 238000002744 homologous recombination Methods 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Claims (8)
1. Molekula nukleinske kiseline, koja ima nukleotidnu sekvencu prema identifikacijskom broju (SEQ ID NO.) 2, koja je operabilno vezana za gen koji kodira diaminopimelat dehidrogeazu.
2. Vektor, koji obuhvaća molekulu nukleinske kiseline prema zahtjevu 1.
3. Transformant, u kojem je usidren vektor prema zahtjevu 2.
4. Transformant prema zahtjevu 3, koji pripada rodu Corynebacterium ili rodu Brevibacterium.
5. Transformant prema zahtjevu 3, imenovan kao CA01-0136 i položen s KCCM pod pristupnim brojem KCCM10920P.
6. Transformant prema zahtjevu 3 ili 4, gdje je molekula nukleinske kiseline prema zahtjevu 1 inkorporirana u genom transformanta preko homologne rekombinacije.
7. Transformant prema zahtjevu 3 ili 4, u kojem je usidrena molekula nukleinske kiseline prema zahtjevu 1 kao plazmid.
8. Postupak proizvodnje lizina, koji obuhvaća fermentaciju transformanta prema bilo kojem od zahtjeva 3 do 7, i dalje obuhvaća skupljanje proizvedenog lizina.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020080010073A KR100987281B1 (ko) | 2008-01-31 | 2008-01-31 | 개량된 프로모터 및 이를 이용한 ｌ-라이신의 생산 방법 |
| PCT/KR2009/000382 WO2009096690A2 (ko) | 2008-01-31 | 2009-01-23 | 개량된 프로모터 및 이를 이용한 ｌ-라이신의 생산 방법 |
| EP09705987.7A EP2236610B1 (en) | 2008-01-31 | 2009-01-23 | Improved promoter, and a production method for l-lysine using the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20161224T1 true HRP20161224T1 (hr) | 2016-12-02 |
Family
ID=40913400
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20161224TT HRP20161224T1 (hr) | 2008-01-31 | 2016-09-26 | Poboljšani promotor, i postupak proizvodnje l-lizina uporabom istog |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US8535915B2 (hr) |
| EP (3) | EP2993233B1 (hr) |
| JP (1) | JP5400065B2 (hr) |
| KR (1) | KR100987281B1 (hr) |
| CN (2) | CN103555721A (hr) |
| BR (1) | BRPI0906584B1 (hr) |
| DK (2) | DK2236610T3 (hr) |
| ES (2) | ES2597877T3 (hr) |
| HR (1) | HRP20161224T1 (hr) |
| HU (2) | HUE044749T2 (hr) |
| LT (1) | LT2236610T (hr) |
| PL (2) | PL2236610T3 (hr) |
| WO (1) | WO2009096690A2 (hr) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101226384B1 (ko) * | 2010-03-05 | 2013-01-25 | 씨제이제일제당 (주) | 개량된 프로모터 및 이를 이용한 ｌ-라이신의 생산 방법 |
| DE102010019059A1 (de) * | 2010-05-03 | 2011-11-03 | Forschungszentrum Jülich GmbH | Sensoren zur intrazellulären Metabolit-Detektion |
| KR101335853B1 (ko) | 2011-12-01 | 2013-12-02 | 씨제이제일제당 (주) | Ｌ-아미노산 및 리보플라빈을 동시에 생산하는 미생물 및 이를 이용한 ｌ-아미노산 및 리보플라빈을 생산하는 방법 |
| EP2796555B1 (en) | 2011-12-21 | 2018-08-29 | Cj Cheiljedang Corporation | Method for producing l-lysine using microorganisms having ability to produce l-lysine |
| KR20140066553A (ko) | 2012-11-23 | 2014-06-02 | 삼성전자주식회사 | 코리네박테리움 속 균주의 신규 프로모터 |
| KR101793328B1 (ko) * | 2015-07-03 | 2017-11-03 | 씨제이제일제당 (주) | Ｌ-라이신 생산능을 갖는 미생물 및 이를 이용한 ｌ-라이신 생산 방법 |
| CN110283823B (zh) * | 2016-08-31 | 2023-05-12 | Cj第一制糖株式会社 | 新型启动子及其应用 |
| KR101925930B1 (ko) * | 2017-07-24 | 2019-02-26 | 씨제이제일제당 주식회사 | Ｌ-라이신을 생산하는 미생물 및 이를 이용한 ｌ-라이신의 생산 방법 |
| CN113201536B (zh) | 2020-08-19 | 2022-09-13 | 中国科学院天津工业生物技术研究所 | 具有启动子活性的多核苷酸及其在生产氨基酸中的应用 |
| WO2022265130A1 (ko) * | 2021-06-15 | 2022-12-22 | 씨제이제일제당 (주) | 신규 프로모터 및 이의 용도 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3036912B2 (ja) | 1991-09-02 | 2000-04-24 | 協和醗酵工業株式会社 | 遺伝子発現調節ｄｎａ |
| JPH07155184A (ja) | 1993-12-08 | 1995-06-20 | Ajinomoto Co Inc | 発酵法によるｌ−リジンの製造法 |
| DE4440118C1 (de) | 1994-11-11 | 1995-11-09 | Forschungszentrum Juelich Gmbh | Die Genexpression in coryneformen Bakterien regulierende DNA |
| KR0159812B1 (ko) | 1995-12-20 | 1998-11-16 | 손경식 | 코리네박테리움 글루타미컴 씨에이치 77 및 이 균주를 이용한 l-라이신의 제조 방법 |
| JP4075087B2 (ja) | 1996-12-05 | 2008-04-16 | 味の素株式会社 | Ｌ−リジンの製造法 |
| US20020086371A1 (en) | 1999-07-07 | 2002-07-04 | Degussa-Huls Aktiengesellschaft | L-lysine-producing corynebacteria and process for the preparation of L-lysine |
| BR0012966A (pt) * | 1999-08-02 | 2002-05-14 | Archer Daniels Midland Co | Produção de l-lisina de cepas bacterianas mutantes |
| JP4623825B2 (ja) | 1999-12-16 | 2011-02-02 | 協和発酵バイオ株式会社 | 新規ポリヌクレオチド |
| US6927046B1 (en) | 1999-12-30 | 2005-08-09 | Archer-Daniels-Midland Company | Increased lysine production by gene amplification using coryneform bacteria |
| DE60045191D1 (de) | 2000-03-09 | 2010-12-16 | Evonik Degussa Gmbh | Corynebacterium glutamicum gene, die für stoffwechselproteine kodieren |
| US7141388B2 (en) | 2000-11-15 | 2006-11-28 | Archer-Daniels-Midland Company | Nucleotide sequences for transcriptional regulation in corynebacterium glutamicum |
| KR100397322B1 (ko) | 2000-12-30 | 2003-09-06 | 씨제이 주식회사 | 엘-라이신의 제조방법 |
| EP1414970A2 (en) * | 2001-08-06 | 2004-05-06 | Degussa AG | Production of l-lysine by genetically modified corynebacterium glutamicum strains |
| KR100620092B1 (ko) | 2004-12-16 | 2006-09-08 | 씨제이 주식회사 | 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법 |
| KR100653742B1 (ko) | 2004-12-30 | 2006-12-05 | 씨제이 주식회사 | 신규한 ｌ-라이신-유도성 프로모터 |
| WO2008033001A1 (en) | 2006-09-15 | 2008-03-20 | Cj Cheiljedang Corporation | A corynebacteria having enhanced l-lysine productivity and a method of producing l-lysine using the same |
-
2008
- 2008-01-31 KR KR1020080010073A patent/KR100987281B1/ko active IP Right Review Request
-
2009
- 2009-01-23 ES ES09705987.7T patent/ES2597877T3/es active Active
- 2009-01-23 HU HUE15184909 patent/HUE044749T2/hu unknown
- 2009-01-23 PL PL09705987T patent/PL2236610T3/pl unknown
- 2009-01-23 PL PL15184909T patent/PL2993233T3/pl unknown
- 2009-01-23 ES ES15184909T patent/ES2743422T3/es active Active
- 2009-01-23 HU HUE09705987A patent/HUE030771T2/en unknown
- 2009-01-23 CN CN201210322618.0A patent/CN103555721A/zh active Pending
- 2009-01-23 EP EP15184909.8A patent/EP2993233B1/en active Active
- 2009-01-23 US US12/864,979 patent/US8535915B2/en active Active
- 2009-01-23 WO PCT/KR2009/000382 patent/WO2009096690A2/ko active Application Filing
- 2009-01-23 EP EP09705987.7A patent/EP2236610B1/en active Active
- 2009-01-23 DK DK09705987.7T patent/DK2236610T3/en active
- 2009-01-23 BR BRPI0906584-9A patent/BRPI0906584B1/pt active IP Right Grant
- 2009-01-23 CN CN2009801033156A patent/CN101939432B/zh active Active
- 2009-01-23 LT LTEP09705987.7T patent/LT2236610T/lt unknown
- 2009-01-23 DK DK15184909.8T patent/DK2993233T3/da active
- 2009-01-23 JP JP2010544884A patent/JP5400065B2/ja active Active
- 2009-01-23 EP EP15184911.4A patent/EP2990488A1/en not_active Withdrawn
-
2016
- 2016-09-26 HR HRP20161224TT patent/HRP20161224T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| HUE030771T2 (en) | 2017-05-29 |
| DK2993233T3 (da) | 2019-08-19 |
| ES2597877T3 (es) | 2017-01-23 |
| PL2993233T3 (pl) | 2019-11-29 |
| KR100987281B1 (ko) | 2010-10-12 |
| WO2009096690A2 (ko) | 2009-08-06 |
| BRPI0906584B1 (pt) | 2020-12-01 |
| KR20090084099A (ko) | 2009-08-05 |
| EP2993233B1 (en) | 2019-06-19 |
| PL2236610T3 (pl) | 2017-01-31 |
| JP2011510651A (ja) | 2011-04-07 |
| BRPI0906584A2 (pt) | 2019-10-01 |
| EP2236610A2 (en) | 2010-10-06 |
| JP5400065B2 (ja) | 2014-01-29 |
| EP2236610B1 (en) | 2016-07-20 |
| US20110076731A1 (en) | 2011-03-31 |
| HUE044749T2 (hu) | 2019-11-28 |
| CN103555721A (zh) | 2014-02-05 |
| CN101939432B (zh) | 2013-02-13 |
| DK2236610T3 (en) | 2016-11-14 |
| EP2236610A4 (en) | 2011-03-16 |
| EP2990488A1 (en) | 2016-03-02 |
| ES2743422T3 (es) | 2020-02-19 |
| CN101939432A (zh) | 2011-01-05 |
| US8535915B2 (en) | 2013-09-17 |
| LT2236610T (lt) | 2016-10-25 |
| EP2993233A1 (en) | 2016-03-09 |
| WO2009096690A3 (ko) | 2009-10-29 |
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