WO2009096690A3 - 개량된 프로모터 및 이를 이용한 ｌ-라이신의 생산 방법 - Google Patents

개량된 프로모터 및 이를 이용한 ｌ-라이신의 생산 방법 Download PDF

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Publication number
WO2009096690A3
WO2009096690A3 PCT/KR2009/000382 KR2009000382W WO2009096690A3 WO 2009096690 A3 WO2009096690 A3 WO 2009096690A3 KR 2009000382 W KR2009000382 W KR 2009000382W WO 2009096690 A3 WO2009096690 A3 WO 2009096690A3
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WO
WIPO (PCT)
Prior art keywords
lysine
same
production method
improved promoter
transformant
Prior art date
Application number
PCT/KR2009/000382
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English (en)
French (fr)
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WO2009096690A2 (ko
Inventor
김철하
최종수
임상조
김형준
나소연
전계향
Original Assignee
씨제이제일제당 (주)
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40913400&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2009096690(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to EP09705987.7A priority Critical patent/EP2236610B1/en
Priority to PL15184909T priority patent/PL2993233T3/pl
Priority to EP15184909.8A priority patent/EP2993233B1/en
Priority to LTEP09705987.7T priority patent/LT2236610T/lt
Priority to DK09705987.7T priority patent/DK2236610T3/en
Application filed by 씨제이제일제당 (주) filed Critical 씨제이제일제당 (주)
Priority to ES09705987.7T priority patent/ES2597877T3/es
Priority to CN2009801033156A priority patent/CN101939432B/zh
Priority to US12/864,979 priority patent/US8535915B2/en
Priority to JP2010544884A priority patent/JP5400065B2/ja
Priority to BRPI0906584-9A priority patent/BRPI0906584B1/pt
Publication of WO2009096690A2 publication Critical patent/WO2009096690A2/ko
Publication of WO2009096690A3 publication Critical patent/WO2009096690A3/ko
Priority to HRP20161224TT priority patent/HRP20161224T1/hr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Fodder In General (AREA)

Abstract

본 발명은 디아미노피멜레이트 디히드로게나제를 암호화하는 유전자와 작동가능하게 연결되고, 개량된 프로모터 활성을 갖는 코리네박테리움 글루타미쿰 유래의 핵산 분자, 이러한 핵산 분자를 포함하는 벡터, 이러한 벡터로 형질전환된 형질전환체 및 이러한 형질전환체를 사용하여 L-라이신을 생산하는 방법을 제공한다.
PCT/KR2009/000382 2008-01-31 2009-01-23 개량된 프로모터 및 이를 이용한 ｌ-라이신의 생산 방법 WO2009096690A2 (ko)

Priority Applications (11)

Application Number Priority Date Filing Date Title
BRPI0906584-9A BRPI0906584B1 (pt) 2008-01-31 2009-01-23 promotor aprimorado e método para a produção de l-lisina usando o mesmo
CN2009801033156A CN101939432B (zh) 2008-01-31 2009-01-23 改进的启动子和用其产生l-赖氨酸的方法
EP15184909.8A EP2993233B1 (en) 2008-01-31 2009-01-23 Improved promoter, and a production method for l-lysine using the same
LTEP09705987.7T LT2236610T (lt) 2008-01-31 2009-01-23 Patobulintas promotorius ir jo panaudojimas l-lizino gamybos būdui
DK09705987.7T DK2236610T3 (en) 2008-01-31 2009-01-23 Promoter and improved method for producing L-lysine using the same
EP09705987.7A EP2236610B1 (en) 2008-01-31 2009-01-23 Improved promoter, and a production method for l-lysine using the same
ES09705987.7T ES2597877T3 (es) 2008-01-31 2009-01-23 Promotor mejorado y método para la producción de L-lisina mediante el uso del mismo
PL15184909T PL2993233T3 (pl) 2008-01-31 2009-01-23 Ulepszony promotor oraz sposób wytwarzania L-lizyny przy jego zastosowaniu
US12/864,979 US8535915B2 (en) 2008-01-31 2009-01-23 Promoter, and a production method for L-lysine using the same
JP2010544884A JP5400065B2 (ja) 2008-01-31 2009-01-23 改良されたプロモーターおよびこれを用いたｌ−リシンの生産方法
HRP20161224TT HRP20161224T1 (hr) 2008-01-31 2016-09-26 Poboljšani promotor, i postupak proizvodnje l-lizina uporabom istog

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020080010073A KR100987281B1 (ko) 2008-01-31 2008-01-31 개량된 프로모터 및 이를 이용한 ｌ-라이신의 생산 방법
KR10-2008-0010073 2008-01-31

Publications (2)

Publication Number Publication Date
WO2009096690A2 WO2009096690A2 (ko) 2009-08-06
WO2009096690A3 true WO2009096690A3 (ko) 2009-10-29

Family

ID=40913400

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/000382 WO2009096690A2 (ko) 2008-01-31 2009-01-23 개량된 프로모터 및 이를 이용한 ｌ-라이신의 생산 방법

Country Status (13)

Country Link
US (1) US8535915B2 (ko)
EP (3) EP2236610B1 (ko)
JP (1) JP5400065B2 (ko)
KR (1) KR100987281B1 (ko)
CN (2) CN101939432B (ko)
BR (1) BRPI0906584B1 (ko)
DK (2) DK2236610T3 (ko)
ES (2) ES2597877T3 (ko)
HR (1) HRP20161224T1 (ko)
HU (2) HUE044749T2 (ko)
LT (1) LT2236610T (ko)
PL (2) PL2236610T3 (ko)
WO (1) WO2009096690A2 (ko)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101226384B1 (ko) * 2010-03-05 2013-01-25 씨제이제일제당 (주) 개량된 프로모터 및 이를 이용한 ｌ-라이신의 생산 방법
DE102010019059A1 (de) * 2010-05-03 2011-11-03 Forschungszentrum Jülich GmbH Sensoren zur intrazellulären Metabolit-Detektion
KR101335853B1 (ko) 2011-12-01 2013-12-02 씨제이제일제당 (주) Ｌ-아미노산 및 리보플라빈을 동시에 생산하는 미생물 및 이를 이용한 ｌ-아미노산 및 리보플라빈을 생산하는 방법
DK2796555T3 (en) 2011-12-21 2018-12-10 Cj Cheiljedang Corp Process for the preparation of L-lysine using microorganisms capable of producing L-lysine
KR20140066553A (ko) 2012-11-23 2014-06-02 삼성전자주식회사 코리네박테리움 속 균주의 신규 프로모터
KR101793328B1 (ko) * 2015-07-03 2017-11-03 씨제이제일제당 (주) Ｌ-라이신 생산능을 갖는 미생물 및 이를 이용한 ｌ-라이신 생산 방법
ES2907694T3 (es) * 2016-08-31 2022-04-26 Cj Cheiljedang Corp Nuevo promotor y uso del mismo
KR101925930B1 (ko) * 2017-07-24 2019-02-26 씨제이제일제당 주식회사 Ｌ-라이신을 생산하는 미생물 및 이를 이용한 ｌ-라이신의 생산 방법
CN113201536B (zh) 2020-08-19 2022-09-13 中国科学院天津工业生物技术研究所 具有启动子活性的多核苷酸及其在生产氨基酸中的应用
WO2022265130A1 (ko) * 2021-06-15 2022-12-22 씨제이제일제당 (주) 신규 프로모터 및 이의 용도

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EP1108790A2 (en) * 1999-12-16 2001-06-20 Kyowa Hakko Kogyo Co., Ltd. Novel polynucleotides
WO2001066573A2 (en) * 2000-03-09 2001-09-13 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding metabolic pathway proteins
US6927046B1 (en) * 1999-12-30 2005-08-09 Archer-Daniels-Midland Company Increased lysine production by gene amplification using coryneform bacteria

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JP3036912B2 (ja) 1991-09-02 2000-04-24 協和醗酵工業株式会社 遺伝子発現調節ｄｎａ
JPH07155184A (ja) 1993-12-08 1995-06-20 Ajinomoto Co Inc 発酵法によるｌ−リジンの製造法
DE4440118C1 (de) 1994-11-11 1995-11-09 Forschungszentrum Juelich Gmbh Die Genexpression in coryneformen Bakterien regulierende DNA
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EP1108790A2 (en) * 1999-12-16 2001-06-20 Kyowa Hakko Kogyo Co., Ltd. Novel polynucleotides
US6927046B1 (en) * 1999-12-30 2005-08-09 Archer-Daniels-Midland Company Increased lysine production by gene amplification using coryneform bacteria
WO2001066573A2 (en) * 2000-03-09 2001-09-13 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding metabolic pathway proteins

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Also Published As

Publication number Publication date
DK2993233T3 (da) 2019-08-19
CN103555721A (zh) 2014-02-05
DK2236610T3 (en) 2016-11-14
US20110076731A1 (en) 2011-03-31
PL2236610T3 (pl) 2017-01-31
EP2236610A2 (en) 2010-10-06
KR20090084099A (ko) 2009-08-05
JP5400065B2 (ja) 2014-01-29
HUE030771T2 (en) 2017-05-29
BRPI0906584A2 (pt) 2019-10-01
HUE044749T2 (hu) 2019-11-28
US8535915B2 (en) 2013-09-17
CN101939432B (zh) 2013-02-13
EP2993233A1 (en) 2016-03-09
ES2597877T3 (es) 2017-01-23
CN101939432A (zh) 2011-01-05
EP2990488A1 (en) 2016-03-02
HRP20161224T1 (hr) 2016-12-02
WO2009096690A2 (ko) 2009-08-06
JP2011510651A (ja) 2011-04-07
EP2993233B1 (en) 2019-06-19
KR100987281B1 (ko) 2010-10-12
EP2236610B1 (en) 2016-07-20
ES2743422T3 (es) 2020-02-19
BRPI0906584B1 (pt) 2020-12-01
PL2993233T3 (pl) 2019-11-29
EP2236610A4 (en) 2011-03-16
LT2236610T (lt) 2016-10-25

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