WO2008146745A1 - 新規なリゾホスファチジン酸アシル基転移酵素遺伝子 - Google Patents

新規なリゾホスファチジン酸アシル基転移酵素遺伝子 Download PDF

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Publication number
WO2008146745A1
WO2008146745A1 PCT/JP2008/059564 JP2008059564W WO2008146745A1 WO 2008146745 A1 WO2008146745 A1 WO 2008146745A1 JP 2008059564 W JP2008059564 W JP 2008059564W WO 2008146745 A1 WO2008146745 A1 WO 2008146745A1
Authority
WO
WIPO (PCT)
Prior art keywords
novel
acyltransferase gene
lysophosphatidate acyltransferase
lysophosphatidate
gene
Prior art date
Application number
PCT/JP2008/059564
Other languages
English (en)
French (fr)
Inventor
Misa Ochiai
Hisanori Tokuda
Original Assignee
Suntory Holdings Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suntory Holdings Limited filed Critical Suntory Holdings Limited
Priority to JP2009516300A priority Critical patent/JP5371749B2/ja
Priority to CA2687610A priority patent/CA2687610C/en
Priority to EP08764610.5A priority patent/EP2169055B1/en
Priority to AU2008255877A priority patent/AU2008255877B2/en
Priority to KR1020097022814A priority patent/KR101496803B1/ko
Priority to DK08764610.5T priority patent/DK2169055T3/da
Priority to BRPI0809994-4A2A priority patent/BRPI0809994A2/pt
Priority to RU2009148322/10A priority patent/RU2507263C2/ru
Priority to US12/303,320 priority patent/US8110388B2/en
Priority to CN2008800002419A priority patent/CN101541963B/zh
Publication of WO2008146745A1 publication Critical patent/WO2008146745A1/ja

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/13—Nucleic acids or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Edible Oils And Fats (AREA)

Abstract

　新規なリゾホスファチジン酸アシル基転移酵素遺伝子の提供。 　配列番号１、３，３６若しくは３７で示される塩基配列又はそのフラグメントを含む核酸。
PCT/JP2008/059564 2007-05-25 2008-05-23 新規なリゾホスファチジン酸アシル基転移酵素遺伝子 WO2008146745A1 (ja)

Priority Applications (10)

Application Number Priority Date Filing Date Title
JP2009516300A JP5371749B2 (ja) 2007-05-25 2008-05-23 新規なリゾホスファチジン酸アシル基転移酵素遺伝子
CA2687610A CA2687610C (en) 2007-05-25 2008-05-23 Lysophosphatidic acid acyltransferase genes
EP08764610.5A EP2169055B1 (en) 2007-05-25 2008-05-23 Novel lysophosphatidate acyltransferase gene
AU2008255877A AU2008255877B2 (en) 2007-05-25 2008-05-23 Novel lysophosphatidic acid acyltransferase genes
KR1020097022814A KR101496803B1 (ko) 2007-05-25 2008-05-23 신규 리소포스파티드산 아실기 전이 효소 유전자
DK08764610.5T DK2169055T3 (da) 2007-05-25 2008-05-23 Nyt lysophosphatidat-acyltransferasegen.
BRPI0809994-4A2A BRPI0809994A2 (pt) 2007-05-25 2008-05-23 Genes de ácido lisofosfatídico aciltransferase
RU2009148322/10A RU2507263C2 (ru) 2007-05-25 2008-05-23 Новые гены ацилтрансферазы лизофосфатидной кислоты
US12/303,320 US8110388B2 (en) 2007-05-25 2008-05-23 Lysophosphatidic acid acyltransferase genes
CN2008800002419A CN101541963B (zh) 2007-05-25 2008-05-23 新型溶血磷脂酸酰基转移酶基因

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007139046 2007-05-25
JP2007-139046 2007-05-25
JP2007323965 2007-12-14
JP2007-323965 2007-12-14

Publications (1)

Publication Number Publication Date
WO2008146745A1 true WO2008146745A1 (ja) 2008-12-04

Family

ID=40316833

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/059564 WO2008146745A1 (ja) 2007-05-25 2008-05-23 新規なリゾホスファチジン酸アシル基転移酵素遺伝子

Country Status (11)

Country Link
US (1) US8110388B2 (ja)
EP (1) EP2169055B1 (ja)
JP (1) JP5371749B2 (ja)
KR (1) KR101496803B1 (ja)
CN (1) CN101541963B (ja)
AU (1) AU2008255877B2 (ja)
BR (1) BRPI0809994A2 (ja)
CA (1) CA2687610C (ja)
DK (1) DK2169055T3 (ja)
RU (1) RU2507263C2 (ja)
WO (1) WO2008146745A1 (ja)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010110375A1 (ja) 2009-03-26 2010-09-30 サントリーホールディングス株式会社 新規なリゾリン脂質アシル基転移酵素
US7879591B2 (en) 2004-11-04 2011-02-01 E.I. Du Pont De Nemours And Company High eicosapentaenoic acid producing strains of Yarrowia lipolytica
US8110388B2 (en) 2007-05-25 2012-02-07 Suntory Holdings Limited Lysophosphatidic acid acyltransferase genes
US8524485B2 (en) 2009-06-16 2013-09-03 E I Du Pont De Nemours And Company Long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100196579A1 (en) * 2007-07-11 2010-08-05 Suntory Holdings Limited Phosphatidic acid phosphatase homologs and use thereof
KR101399242B1 (ko) 2009-09-18 2014-05-27 산토리 홀딩스 가부시키가이샤 글리세롤-3-인산 아실기 전이 효소
CA2783543C (en) 2009-12-21 2016-01-26 Suntory Holdings Limited Diacylglycerol acyltransferase genes and use thereof
JP5890687B2 (ja) 2010-02-03 2016-03-22 サントリーホールディングス株式会社 グリセロール−３−リン酸アシル基転移酵素ホモログとその利用
KR101964168B1 (ko) * 2010-10-01 2019-04-01 고쿠리쓰다이가쿠호진 규슈다이가쿠 스트라메노파일의 형질 전환 방법
RU2678420C2 (ru) 2013-03-27 2019-01-28 Сантори Холдингз Лимитед Промотор, проявляющий высокую экспрессионную активность в микроорганизмах mortierella
WO2015031335A1 (en) 2013-08-28 2015-03-05 Brookhaven Science Associates Llc Engineering cyclopropane fatty acid accumulation in plants
CN105924512B (zh) * 2016-07-12 2019-08-23 中国农业科学院棉花研究所 一种与油脂含量相关的GhLPAAT5-like蛋白及其编码基因与应用
CN113980997A (zh) * 2021-09-17 2022-01-28 中北大学 测定溶血磷脂酸酰基转移酶动力学参数的方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001012780A1 (fr) * 1999-08-13 2001-02-22 Suntory Limited Micro-organisme secretant un lipide et procede de production de ce lipide et de capsules contenant ce lipide au moyen de ce micro-organisme
WO2005019437A1 (ja) * 2003-08-22 2005-03-03 Suntory Limited 脂質生産菌の育種方法
US20060094090A1 (en) * 2004-11-04 2006-05-04 Damude Howard G Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US20060094092A1 (en) * 2004-11-04 2006-05-04 Damude Howard G High arachidonic acid producing strains of Yarrowia lipolytica

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040022449A (ko) * 2001-07-12 2004-03-12 유니버시티 오브 매사추세츠 유전자 불활성화를 매개하는 소형 간섭 ｒｎａ의 생체내제조
EP1599582B1 (de) 2003-02-27 2011-07-27 BASF Plant Science GmbH Verfahren zur herstellung mehrfach ungesättigter fettsäuren
EP1613746B1 (de) 2003-03-31 2013-03-06 University Of Bristol Neue pflanzliche acyltransferasen spezifisch für langkettige, mehrfach ungesättigte fettsäuren
US8119860B2 (en) * 2007-04-16 2012-02-21 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
CA2687610C (en) 2007-05-25 2017-01-24 Suntory Holdings Limited Lysophosphatidic acid acyltransferase genes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001012780A1 (fr) * 1999-08-13 2001-02-22 Suntory Limited Micro-organisme secretant un lipide et procede de production de ce lipide et de capsules contenant ce lipide au moyen de ce micro-organisme
WO2005019437A1 (ja) * 2003-08-22 2005-03-03 Suntory Limited 脂質生産菌の育種方法
US20060094090A1 (en) * 2004-11-04 2006-05-04 Damude Howard G Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US20060094092A1 (en) * 2004-11-04 2006-05-04 Damude Howard G High arachidonic acid producing strains of Yarrowia lipolytica

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2169055A4 *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7879591B2 (en) 2004-11-04 2011-02-01 E.I. Du Pont De Nemours And Company High eicosapentaenoic acid producing strains of Yarrowia lipolytica
US8110388B2 (en) 2007-05-25 2012-02-07 Suntory Holdings Limited Lysophosphatidic acid acyltransferase genes
EP2676554A3 (en) * 2009-03-26 2014-02-26 Suntory Holdings Limited Novel lysophospholipid acyltransferase
KR20110137791A (ko) 2009-03-26 2011-12-23 산토리 홀딩스 가부시키가이샤 신규한 리소인지질 아실기 전이효소
EP2676554A2 (en) 2009-03-26 2013-12-25 Suntory Holdings Limited Novel lysophospholipid acyltransferase
WO2010110375A1 (ja) 2009-03-26 2010-09-30 サントリーホールディングス株式会社 新規なリゾリン脂質アシル基転移酵素
JP5539961B2 (ja) * 2009-03-26 2014-07-02 サントリーホールディングス株式会社 新規なリゾリン脂質アシル基転移酵素
AU2010228217B2 (en) * 2009-03-26 2014-07-03 Suntory Holdings Limited Novel lysophospholipid acyltransferase
US8790906B2 (en) 2009-03-26 2014-07-29 Suntory Holdings Limited Lysophospholipid acyltransferase
US9315835B2 (en) 2009-03-26 2016-04-19 Suntory Holdings Limited Lysophospholipid acyltransferase
KR20170002700A (ko) 2009-03-26 2017-01-06 산토리 홀딩스 가부시키가이샤 신규한 리소인지질 아실기 전이효소
US8524485B2 (en) 2009-06-16 2013-09-03 E I Du Pont De Nemours And Company Long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases
US9029122B2 (en) 2009-06-16 2015-05-12 E I Du Pont De Nemours And Company Long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases

Also Published As

Publication number Publication date
JP5371749B2 (ja) 2013-12-18
AU2008255877B2 (en) 2013-10-31
KR20100023796A (ko) 2010-03-04
EP2169055A1 (en) 2010-03-31
RU2507263C2 (ru) 2014-02-20
EP2169055A4 (en) 2011-06-22
AU2008255877A1 (en) 2008-12-04
RU2009148322A (ru) 2011-06-27
JPWO2008146745A1 (ja) 2010-08-19
CN101541963B (zh) 2012-10-31
CN101541963A (zh) 2009-09-23
DK2169055T3 (da) 2015-06-22
EP2169055B1 (en) 2015-04-01
KR101496803B1 (ko) 2015-03-06
US20100203218A1 (en) 2010-08-12
CA2687610C (en) 2017-01-24
US8110388B2 (en) 2012-02-07
BRPI0809994A2 (pt) 2014-10-07
CA2687610A1 (en) 2008-12-04

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