HRP20160966T1 - Postupak za odvajanje jezgrenih stanica izvedenih iz koštane srži u svrhu izgradnje kosti - Google Patents
Postupak za odvajanje jezgrenih stanica izvedenih iz koštane srži u svrhu izgradnje kosti Download PDFInfo
- Publication number
- HRP20160966T1 HRP20160966T1 HRP20160966TT HRP20160966T HRP20160966T1 HR P20160966 T1 HRP20160966 T1 HR P20160966T1 HR P20160966T T HRP20160966T T HR P20160966TT HR P20160966 T HRP20160966 T HR P20160966T HR P20160966 T1 HRP20160966 T1 HR P20160966T1
- Authority
- HR
- Croatia
- Prior art keywords
- bone marrow
- buffer
- bone
- steps
- solution
- Prior art date
Links
- 210000001185 bone marrow Anatomy 0.000 title claims 14
- 230000011164 ossification Effects 0.000 title 1
- 238000000926 separation method Methods 0.000 title 1
- 210000004027 cell Anatomy 0.000 claims 9
- 239000000872 buffer Substances 0.000 claims 8
- 239000000243 solution Substances 0.000 claims 6
- 238000005119 centrifugation Methods 0.000 claims 4
- 238000012423 maintenance Methods 0.000 claims 4
- 239000000463 material Substances 0.000 claims 4
- 230000037176 bone building Effects 0.000 claims 3
- 239000006166 lysate Substances 0.000 claims 3
- 239000011534 wash buffer Substances 0.000 claims 3
- 210000000988 bone and bone Anatomy 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 230000009089 cytolysis Effects 0.000 claims 1
- 230000002950 deficient Effects 0.000 claims 1
- 210000003743 erythrocyte Anatomy 0.000 claims 1
- 239000012535 impurity Substances 0.000 claims 1
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 230000003902 lesion Effects 0.000 claims 1
- 230000003472 neutralizing effect Effects 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
Claims (2)
1. Postupak za odvajanje jezgrenih stanica izvedenih iz koštane srži za izgradnju kosti, naznačen time, da obuhvaća sljedeće korake:
(a) ispiranje koštane srži, koje sadrži svoje sljedeće korake:
(a1) pohrana 2-5 ml koštane srži izvađene iz pacijenta, u puferu za ispiranje;
(a2) protresanje pufera za ispiranje s koštanom srži od 40 do 50 puta, kako bi se dovoljno odstranile nečistoće ili masni sastojci iz koštane srži;
(a3) centrifugiranje protresanog pufera za ispiranje na 1600 rpm u vremenu od 4 do 5 minuta;
(a4) odstranjivanje materijala koji je isplivao na površinu;
(a5) prebacivanje preostalog sastojka koštane srži u drugi spremnik za ispiranje;
(a6) protresanje spremnika koji sadrži koštanu srž od 40 do 50 puta;
(a7) centrifugiranje protresane otopine na 1600 rpm u vremenu od 4 do 5 minuta; i
(a8) odstranjivanje materijala koji je isplivao na površinu od centrifugirane otopine;
(b) liziranje crvenih krvnih stanica u koštanoj srži, koje sadrži svoje sljedeće korake:
(b1) dodavanje deseterostruke količine lizatnog pufera u koštanu srž;
(b2) miješanje lizatnog pufera s koštanom srži nekoliko puta uz pomoć štrcaljke; i
(b3) ostavljanje otopine da odstoji u vremenu od 10 do 20 sekundi;
(c) neutraliziranje lizata koštane srži, koje sadrži svoje sljedeće korake:
(c1) miješanje otopine koštane srži s istom količinom neutraliziranog pufera;
(c2) centrifugiranje navedene otopine na 1600 rpm u vremenu od 4 do 5 minuta; i
(c3) odstranjivanje materijala koji je isplivao na površinu osim nataloženih jezgrenih stanica za izgradnju kostiju;
(d) pročišćavanje jezgrenih stanica iz neutralizirane koštane srži, koje sadrži svoje sljedeće korake:
(d1) miješanje 4-5 ml održavanog pufera s jezgrenim stanicama u spremniku;
(d2) centrifugiranje navedene otopine na 1600 rpm u vremenu od 4 do 5 minuta; i
(d3) odstranjivanje materijala koji je isplivao na površinu;
i
(e) miješanje jezgrenih stanica s održavanim puferom u svrhu izgradnje kostiju.
2. Postupak prema zahtjevu 1, naznačen time, da korak (e) miješanje jezgrenih stanica s održavanim puferom, sadrži svoje sljedeće korake:
(e1) dodavanje 2-3 ml održavanog pufera u jezgrene stanice u vidu lezije;
(e2) ubrizgavanje jezgrenih stanica tako da plutaju na dnu spremnika uz pomoć štrcaljke; i
(e3) stavljanje jezgrenih stanica i održavanog pufera u štrcaljku za ubrizgavanje u defektno mjesto na kosti ili na mjesto kojem je potrebna izgradnja kosti.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020060043936A KR100833793B1 (ko) | 2006-05-16 | 2006-05-16 | 골수 유래 골 생성용 유핵 세포 분리 방법 |
| PCT/KR2006/002434 WO2007132962A1 (en) | 2006-05-16 | 2006-06-23 | Separation method of nucleated cells derived from bone marrow for bone formation |
| EP06769014.9A EP2018419B1 (en) | 2006-05-16 | 2006-06-23 | Separation method of nucleated cells derived from bone marrow for bone formation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20160966T1 true HRP20160966T1 (hr) | 2016-11-18 |
Family
ID=38694041
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20160966TT HRP20160966T1 (hr) | 2006-05-16 | 2016-07-28 | Postupak za odvajanje jezgrenih stanica izvedenih iz koštane srži u svrhu izgradnje kosti |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US20090111087A1 (hr) |
| EP (1) | EP2018419B1 (hr) |
| JP (1) | JP2009537215A (hr) |
| KR (1) | KR100833793B1 (hr) |
| CN (1) | CN101432421A (hr) |
| BR (1) | BRPI0621573A2 (hr) |
| CY (1) | CY1117747T1 (hr) |
| DK (1) | DK2018419T3 (hr) |
| ES (1) | ES2585231T3 (hr) |
| HR (1) | HRP20160966T1 (hr) |
| HU (1) | HUE029896T2 (hr) |
| PL (1) | PL2018419T3 (hr) |
| PT (1) | PT2018419T (hr) |
| RS (1) | RS54893B1 (hr) |
| SG (2) | SG172595A1 (hr) |
| SI (1) | SI2018419T1 (hr) |
| WO (1) | WO2007132962A1 (hr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104774805A (zh) * | 2015-04-03 | 2015-07-15 | 银广悦 | 一种分离骨髓单个核细胞的方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0877795A4 (en) * | 1996-01-16 | 2003-03-05 | Depuy Orthopaedics Inc | INSULATION OF PROCUREMENT CELLS FROM HEMATOPOIETIC AND NON-HEMATOPOIETIC TISSUES AND THEIR USE |
| US5824084A (en) * | 1996-07-03 | 1998-10-20 | The Cleveland Clinic Foundation | Method of preparing a composite bone graft |
| EP1185867B1 (en) * | 1999-05-28 | 2005-03-30 | Stemcell Technologies Inc. | Method for separating cells using immunorosettes |
| JP3929283B2 (ja) * | 2000-11-08 | 2007-06-13 | シスメックス株式会社 | 骨髄有核細胞の分類計数方法 |
| US7049093B2 (en) * | 2000-11-08 | 2006-05-23 | Sysmex Corporation | Method of classifying and counting nucleated bone marrow cells |
| US6723131B2 (en) * | 2001-02-28 | 2004-04-20 | The Cleveland Clinic Foundation | Composite bone marrow graft material with method and kit |
| CN1276252C (zh) * | 2001-07-27 | 2006-09-20 | 贝克曼库尔特有限公司 | 有核红细胞的测量方法 |
| AU2002353443A1 (en) * | 2001-11-07 | 2003-05-19 | Bioview Ltd. | Kits and methods for preparing cell samples optimized for dual staining |
| KR20030085257A (ko) * | 2002-04-29 | 2003-11-05 | 주식회사 셀론텍 | 유핵세포 분리용 혈액백 및 이를 이용한 유핵세포 분리방법 |
| JP4087161B2 (ja) * | 2002-06-21 | 2008-05-21 | シスメックス株式会社 | リンパ球サブセットの異常を検出する方法 |
| WO2004042401A1 (ja) * | 2002-11-08 | 2004-05-21 | Hiroshi Takahashi | 癌細胞の検査方法及びそのための試薬 |
| JP2006055106A (ja) * | 2004-08-23 | 2006-03-02 | National Institute Of Advanced Industrial & Technology | ヒト血清培地を用いるヒト骨髄由来間葉系幹細胞培養法 |
-
2006
- 2006-05-16 KR KR1020060043936A patent/KR100833793B1/ko active IP Right Grant
- 2006-06-23 ES ES06769014.9T patent/ES2585231T3/es active Active
- 2006-06-23 SG SG2011035201A patent/SG172595A1/en unknown
- 2006-06-23 EP EP06769014.9A patent/EP2018419B1/en active Active
- 2006-06-23 SI SI200632077A patent/SI2018419T1/sl unknown
- 2006-06-23 WO PCT/KR2006/002434 patent/WO2007132962A1/en active Application Filing
- 2006-06-23 US US12/226,472 patent/US20090111087A1/en not_active Abandoned
- 2006-06-23 DK DK06769014.9T patent/DK2018419T3/en active
- 2006-06-23 BR BRPI0621573-4A patent/BRPI0621573A2/pt not_active Application Discontinuation
- 2006-06-23 SG SG10201407593RA patent/SG10201407593RA/en unknown
- 2006-06-23 JP JP2009510876A patent/JP2009537215A/ja active Pending
- 2006-06-23 HU HUE06769014A patent/HUE029896T2/en unknown
- 2006-06-23 PT PT67690149T patent/PT2018419T/pt unknown
- 2006-06-23 CN CNA200680054424XA patent/CN101432421A/zh active Pending
- 2006-06-23 PL PL06769014.9T patent/PL2018419T3/pl unknown
- 2006-06-23 RS RS20160463A patent/RS54893B1/sr unknown
-
2016
- 2016-07-07 CY CY20161100644T patent/CY1117747T1/el unknown
- 2016-07-28 HR HRP20160966TT patent/HRP20160966T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PT2018419T (pt) | 2016-07-14 |
| EP2018419B1 (en) | 2016-05-04 |
| RS54893B1 (sr) | 2016-10-31 |
| WO2007132962A1 (en) | 2007-11-22 |
| HUE029896T2 (en) | 2017-04-28 |
| SG172595A1 (en) | 2011-07-28 |
| CN101432421A (zh) | 2009-05-13 |
| KR20070111073A (ko) | 2007-11-21 |
| EP2018419A4 (en) | 2010-07-14 |
| BRPI0621573A2 (pt) | 2011-12-13 |
| EP2018419A1 (en) | 2009-01-28 |
| JP2009537215A (ja) | 2009-10-29 |
| SG10201407593RA (en) | 2014-12-30 |
| KR100833793B1 (ko) | 2008-05-29 |
| ES2585231T3 (es) | 2016-10-04 |
| DK2018419T3 (en) | 2016-08-15 |
| US20090111087A1 (en) | 2009-04-30 |
| PL2018419T3 (pl) | 2016-11-30 |
| CY1117747T1 (el) | 2017-05-17 |
| SI2018419T1 (sl) | 2016-08-31 |
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