SG10201407593RA - Separation method of nucleated cells derived from bone marrow for bone formation - Google Patents

Separation method of nucleated cells derived from bone marrow for bone formation

Info

Publication number
SG10201407593RA
SG10201407593RA SG10201407593RA SG10201407593RA SG10201407593RA SG 10201407593R A SG10201407593R A SG 10201407593RA SG 10201407593R A SG10201407593R A SG 10201407593RA SG 10201407593R A SG10201407593R A SG 10201407593RA SG 10201407593R A SG10201407593R A SG 10201407593RA
Authority
SG
Singapore
Prior art keywords
bone
bone marrow
nucleated cells
bone formation
formation
Prior art date
Application number
SG10201407593RA
Inventor
Hyun-Shin Park
Jae-Deog Jang
Sae-Bom Lee
Cheong-Ho Chang
Original Assignee
Sewon Cellontech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sewon Cellontech Co Ltd filed Critical Sewon Cellontech Co Ltd
Publication of SG10201407593RA publication Critical patent/SG10201407593RA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Rheumatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

9 SEPARATION METHOD OF NUCLEATED CELLS DERIVED FROM BONE MARROW FOR BONE The present invention relates to a method for separating bone marrow-derived nucleated cells for bone formation. The method comprises: the steps of: washing bone marrow; lysing red blood cells in the bone marrow; neutralizing the bone marrow lysate; purifying nucleated cells from the neutralized bone marrow; and mixing the nucleated cells with maintenance a buffer for bone formation. According to the invention, bone marrow-derived nucleated cells, which can be grafted into a site in need of bone formation or bone defect treatment, can be separated in a rapid and convenient manner. As a result, bone formation in emergency patients or patients, who require repeated surgical operations, can be effectively achieved by separating the nucleated cells for bone formation in surgical locations in a convenient and rapid manner and injecting the separated cells into the patients. No suitable figure
SG10201407593RA 2006-05-16 2006-06-23 Separation method of nucleated cells derived from bone marrow for bone formation SG10201407593RA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020060043936A KR100833793B1 (en) 2006-05-16 2006-05-16 Separation method of nucleated cells derived from bone marrow for bone formation

Publications (1)

Publication Number Publication Date
SG10201407593RA true SG10201407593RA (en) 2014-12-30

Family

ID=38694041

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201407593RA SG10201407593RA (en) 2006-05-16 2006-06-23 Separation method of nucleated cells derived from bone marrow for bone formation
SG2011035201A SG172595A1 (en) 2006-05-16 2006-06-23 Separation method of nucleated cells derived from bone marrow for bone formation

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG2011035201A SG172595A1 (en) 2006-05-16 2006-06-23 Separation method of nucleated cells derived from bone marrow for bone formation

Country Status (17)

Country Link
US (1) US20090111087A1 (en)
EP (1) EP2018419B1 (en)
JP (1) JP2009537215A (en)
KR (1) KR100833793B1 (en)
CN (1) CN101432421A (en)
BR (1) BRPI0621573A2 (en)
CY (1) CY1117747T1 (en)
DK (1) DK2018419T3 (en)
ES (1) ES2585231T3 (en)
HR (1) HRP20160966T1 (en)
HU (1) HUE029896T2 (en)
PL (1) PL2018419T3 (en)
PT (1) PT2018419T (en)
RS (1) RS54893B1 (en)
SG (2) SG10201407593RA (en)
SI (1) SI2018419T1 (en)
WO (1) WO2007132962A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104774805A (en) * 2015-04-03 2015-07-15 银广悦 Method for separating bone marrow mononuclear cells

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997026326A1 (en) * 1996-01-16 1997-07-24 Depuy Orthopaedics, Inc. Isolation of precursor cells from hematopoietic and non-hematopoietic tissues and their use
US5824084A (en) * 1996-07-03 1998-10-20 The Cleveland Clinic Foundation Method of preparing a composite bone graft
JP4526749B2 (en) * 1999-05-28 2010-08-18 ステムセル テクノロジース インコーポレーテッド Cell separation method using immuno rosette
US7049093B2 (en) * 2000-11-08 2006-05-23 Sysmex Corporation Method of classifying and counting nucleated bone marrow cells
JP3929283B2 (en) * 2000-11-08 2007-06-13 シスメックス株式会社 Bone marrow nucleated cell classification and counting method
US6723131B2 (en) * 2001-02-28 2004-04-20 The Cleveland Clinic Foundation Composite bone marrow graft material with method and kit
JP2005506525A (en) * 2001-07-27 2005-03-03 ベックマン コールター，インコーポレーテッド Methods for measuring nucleated red blood cells
WO2003040064A2 (en) * 2001-11-07 2003-05-15 Bioview Ltd. Kits and methods for preparing cell samples optimized for dual staining
KR20030085257A (en) * 2002-04-29 2003-11-05 주식회사 셀론텍 A blood bag and method for separating cell using of it
JP4087161B2 (en) * 2002-06-21 2008-05-21 シスメックス株式会社 Methods for detecting abnormalities in lymphocyte subsets
AU2003277610A1 (en) * 2002-11-08 2004-06-07 Shuichi Hanada Method of examining cancer cells and reagent therefor
JP2006055106A (en) * 2004-08-23 2006-03-02 National Institute Of Advanced Industrial & Technology Method for culturing mesenchymal stem cell derived from human bone marrow by using human serum culture medium

Also Published As

Publication number Publication date
EP2018419A1 (en) 2009-01-28
EP2018419B1 (en) 2016-05-04
DK2018419T3 (en) 2016-08-15
ES2585231T3 (en) 2016-10-04
CY1117747T1 (en) 2017-05-17
HUE029896T2 (en) 2017-04-28
PT2018419T (en) 2016-07-14
HRP20160966T1 (en) 2016-11-18
US20090111087A1 (en) 2009-04-30
EP2018419A4 (en) 2010-07-14
RS54893B1 (en) 2016-10-31
CN101432421A (en) 2009-05-13
JP2009537215A (en) 2009-10-29
KR20070111073A (en) 2007-11-21
KR100833793B1 (en) 2008-05-29
PL2018419T3 (en) 2016-11-30
SI2018419T1 (en) 2016-08-31
WO2007132962A1 (en) 2007-11-22
SG172595A1 (en) 2011-07-28
BRPI0621573A2 (en) 2011-12-13

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