RS54893B1 - Postupak odvajanja ćelija sa jedrom dobijenih iz koštane srži za izgradnju koštanog tkiva - Google Patents

Postupak odvajanja ćelija sa jedrom dobijenih iz koštane srži za izgradnju koštanog tkiva

Info

Publication number
RS54893B1
RS54893B1 RS20160463A RSP20160463A RS54893B1 RS 54893 B1 RS54893 B1 RS 54893B1 RS 20160463 A RS20160463 A RS 20160463A RS P20160463 A RSP20160463 A RS P20160463A RS 54893 B1 RS54893 B1 RS 54893B1
Authority
RS
Serbia
Prior art keywords
bone marrow
buffer
steps
cells
centrifuging
Prior art date
Application number
RS20160463A
Other languages
English (en)
Inventor
Hyun-Shin Park
Jae-Deog Jang
Sae-Bom Lee
Cheong-Ho Chang
Original Assignee
Sewon Cellontech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sewon Cellontech Co Ltd filed Critical Sewon Cellontech Co Ltd
Publication of RS54893B1 publication Critical patent/RS54893B1/sr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells

Abstract

Postupak za izdvajanje ćelija sa jedrom dobijenih iz koštane srži za izgradnju koštanog tkiva, naznačen time što podrazumeva korake:(a) ispiranja koštane srži, što podrazumeva korake:(a1) prebacivanja 2-5 ml koštane srži prikupljene iz organizma pacijenta u pufer za ispiranje;(a2) mućkanja koštane srži i pufera za ispiranje 40-50 puta kako bi se u dovoljnoj meri uklonile nečistoće ili masne komponente iz koštane srži;(a3) centrifugiranja promućkanog pufera za ispiranje na 1.600 rpm 4-5 minuta;(a4) uklanjanja supernatanta,(a5) prebacivanja preostalog dela koštane srži u drugi sud za ispiranje;(a6) mućkanja suda koji sadrži koštanu srž 40-50 puta,(a7) centrifugiranja promućkanog rastvora na 1.600 rpm 4-5 minuta, i(a8) uklanjanja supernatanta iz centrifugiranog rastvora;(b) liziranja eritrocita u koštanoj srži, što podrazumeva korake:(b1) dodavanja desetostruke količine pufera za liziranje u koštanu srž; i(b2) mešanja pufera za liziranje sa koštanom srži nekoliko puta pomoću šprica, i(b3) ostavljanja rastvora da stoji 10-20 sekundi;(c) neutralisanja lizata koštane srži, što podrazumeva korake:(c1) mešanja rastvora koštane srži sa istom količinom pufera za neutralizaciju;(c2) centrifugiranja promućkanog rastvora na 1.600 rpm 4-5 minuta, i (c3) uklanjanja supernatanta, osim istaloženih ćelija sa jezgrom za izgradnju koštane srži;(d) prečišćavanja ćelija sa jezgrom iz neutralisane koštane srži, što podrazumeva korake:(d1) mešanja 4-5 ml pufera za održavanje sa ćelijama sa jezgrom u posudi;(d2) centrifugiranja navedenog rastvora na 1.600 rpm 4-5 minuta, i (d3) uklanjanja supematanta; i(e) mešanja ćelija sa jedrom sa puferom za održavanje za izgradnju koštane srži.Prijava sadrži još 1 patentni zahtev.
RS20160463A 2006-05-16 2006-06-23 Postupak odvajanja ćelija sa jedrom dobijenih iz koštane srži za izgradnju koštanog tkiva RS54893B1 (sr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020060043936A KR100833793B1 (ko) 2006-05-16 2006-05-16 골수 유래 골 생성용 유핵 세포 분리 방법
PCT/KR2006/002434 WO2007132962A1 (en) 2006-05-16 2006-06-23 Separation method of nucleated cells derived from bone marrow for bone formation
EP06769014.9A EP2018419B1 (en) 2006-05-16 2006-06-23 Separation method of nucleated cells derived from bone marrow for bone formation

Publications (1)

Publication Number Publication Date
RS54893B1 true RS54893B1 (sr) 2016-10-31

Family

ID=38694041

Family Applications (1)

Application Number Title Priority Date Filing Date
RS20160463A RS54893B1 (sr) 2006-05-16 2006-06-23 Postupak odvajanja ćelija sa jedrom dobijenih iz koštane srži za izgradnju koštanog tkiva

Country Status (17)

Country Link
US (1) US20090111087A1 (sr)
EP (1) EP2018419B1 (sr)
JP (1) JP2009537215A (sr)
KR (1) KR100833793B1 (sr)
CN (1) CN101432421A (sr)
BR (1) BRPI0621573A2 (sr)
CY (1) CY1117747T1 (sr)
DK (1) DK2018419T3 (sr)
ES (1) ES2585231T3 (sr)
HR (1) HRP20160966T1 (sr)
HU (1) HUE029896T2 (sr)
PL (1) PL2018419T3 (sr)
PT (1) PT2018419T (sr)
RS (1) RS54893B1 (sr)
SG (2) SG172595A1 (sr)
SI (1) SI2018419T1 (sr)
WO (1) WO2007132962A1 (sr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104774805A (zh) * 2015-04-03 2015-07-15 银广悦 一种分离骨髓单个核细胞的方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0877795A4 (en) * 1996-01-16 2003-03-05 Depuy Orthopaedics Inc INSULATION OF PROCUREMENT CELLS FROM HEMATOPOIETIC AND NON-HEMATOPOIETIC TISSUES AND THEIR USE
US5824084A (en) * 1996-07-03 1998-10-20 The Cleveland Clinic Foundation Method of preparing a composite bone graft
EP1185867B1 (en) * 1999-05-28 2005-03-30 Stemcell Technologies Inc. Method for separating cells using immunorosettes
JP3929283B2 (ja) * 2000-11-08 2007-06-13 シスメックス株式会社 骨髄有核細胞の分類計数方法
US7049093B2 (en) * 2000-11-08 2006-05-23 Sysmex Corporation Method of classifying and counting nucleated bone marrow cells
US6723131B2 (en) * 2001-02-28 2004-04-20 The Cleveland Clinic Foundation Composite bone marrow graft material with method and kit
CN1276252C (zh) * 2001-07-27 2006-09-20 贝克曼库尔特有限公司 有核红细胞的测量方法
AU2002353443A1 (en) * 2001-11-07 2003-05-19 Bioview Ltd. Kits and methods for preparing cell samples optimized for dual staining
KR20030085257A (ko) * 2002-04-29 2003-11-05 주식회사 셀론텍 유핵세포 분리용 혈액백 및 이를 이용한 유핵세포 분리방법
JP4087161B2 (ja) * 2002-06-21 2008-05-21 シスメックス株式会社 リンパ球サブセットの異常を検出する方法
WO2004042401A1 (ja) * 2002-11-08 2004-05-21 Hiroshi Takahashi 癌細胞の検査方法及びそのための試薬
JP2006055106A (ja) * 2004-08-23 2006-03-02 National Institute Of Advanced Industrial & Technology ヒト血清培地を用いるヒト骨髄由来間葉系幹細胞培養法

Also Published As

Publication number Publication date
PT2018419T (pt) 2016-07-14
EP2018419B1 (en) 2016-05-04
WO2007132962A1 (en) 2007-11-22
HRP20160966T1 (hr) 2016-11-18
HUE029896T2 (en) 2017-04-28
SG172595A1 (en) 2011-07-28
CN101432421A (zh) 2009-05-13
KR20070111073A (ko) 2007-11-21
EP2018419A4 (en) 2010-07-14
BRPI0621573A2 (pt) 2011-12-13
EP2018419A1 (en) 2009-01-28
JP2009537215A (ja) 2009-10-29
SG10201407593RA (en) 2014-12-30
KR100833793B1 (ko) 2008-05-29
ES2585231T3 (es) 2016-10-04
DK2018419T3 (en) 2016-08-15
US20090111087A1 (en) 2009-04-30
PL2018419T3 (pl) 2016-11-30
CY1117747T1 (el) 2017-05-17
SI2018419T1 (sl) 2016-08-31

Similar Documents

Publication Publication Date Title
Bakker et al. Mixed languages
Wang et al. Behavior of gingival fibroblasts on titanium implant surfaces in combination with either injectable-PRF or PRP
Chanavaz Maxillary sinus: anatomy, physiology, surgery, and bone grafting related to implantology--eleven years of surgical experience (1979-1990).
Shaw et al. Clinical applications of prenatal and postnatal therapy using stem cells retrieved from amniotic fluid
Mendonça et al. Regenerative facial reconstruction of terminal stage osteoradionecrosis and other advanced craniofacial diseases with adult cultured stem and progenitor cells
Pettersson et al. In vitro osteogenic differentiation of human mesenchymal stem cells from jawbone compared with dental tissue
WO2007067683A3 (en) Methods and compositions for expanding t regulatory cells
WO2007099534A3 (en) Compostions and populations of cells obtained from the umbilical cord and methods of producing the same
JP2016517394A5 (sr)
SG11201901594RA (en) Cell suspension for use in the treatment of lower extremity peripheral artery disease
WO2005011569A3 (en) Methods of using adipose tissue-derived cells in augmenting autologous fat transfer
HK1193428A1 (en) Stem and progenitor cell compositions recovered from bone marrow or cord blood; system and method for preparation thereof
Gorodetsky et al. Allogenic use of human placenta-derived stromal cells as a highly active subtype of mesenchymal stromal cells for cell-based therapies
CN105879120A (zh) 一种天然组织来源的肌腱联合骨脱细胞材料的制备方法
Vickaryous et al. Homology of the reptilian coracoid and a reappraisal of the evolution and development of the amniote pectoral apparatus
Kadkhoda et al. Histopathological comparison between bone marrow-and periodontium-derived stem cells for bone regeneration in rabbit calvaria
RS54893B1 (sr) Postupak odvajanja ćelija sa jedrom dobijenih iz koštane srži za izgradnju koštanog tkiva
Hubbard et al. Aging and transplantation–a topic for biomedicine or bioethics?
Asher et al. New material of Centetodon (Mammalia, Lipotyphla) and the importance of (missing) DNA sequences in systematic paleontology
Sheil Development of the skull of the hawksbill seaturtle, Eretmochelys imbricata
Ruf et al. The chorda tympani and its significance for rodent phylogeny
Vera Candioti et al. Three-dimensional reconstruction of the hyobrancchial apparatus of Hyla nana tadpoles (Anura: hylidae)
El-Serafi Comparison of the Osteogenic Differentiation Potential of Mesenchymal Cells Isolated from Human Bone Marrow, Umbilical Cord Blood and Placenta Derived Stem Cells
Balderrama et al. The optimization of biomaterials in maxillary sinus augmentation associated with bone marrow aspirate concentrate
Pires et al. Repair of Critical Size Bone Defects Using Synthetic Hydroxyapatite or Xenograft with or without the Bone Marrow Mononuclear Fraction: A Histomorphometric and Immunohistochemical Study in Rat Calvaria. Materials 2021, 14, 2854