HRP20141138T1 - Postupak visoke sigurnosti za pripremu proäśišä†enih frakcija matiäśnih stanica - Google Patents
Postupak visoke sigurnosti za pripremu proäśišä†enih frakcija matiäśnih stanica Download PDFInfo
- Publication number
- HRP20141138T1 HRP20141138T1 HRP20141138AT HRP20141138T HRP20141138T1 HR P20141138 T1 HRP20141138 T1 HR P20141138T1 HR P20141138A T HRP20141138A T HR P20141138AT HR P20141138 T HRP20141138 T HR P20141138T HR P20141138 T1 HRP20141138 T1 HR P20141138T1
- Authority
- HR
- Croatia
- Prior art keywords
- syringe
- steps
- stem cells
- aqueous phase
- stem cell
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims 15
- 210000000130 stem cell Anatomy 0.000 title claims 8
- 239000008346 aqueous phase Substances 0.000 claims 4
- 108090000790 Enzymes Proteins 0.000 claims 3
- 102000004190 Enzymes Human genes 0.000 claims 3
- 210000000577 adipose tissue Anatomy 0.000 claims 3
- 239000000463 material Substances 0.000 claims 3
- 239000012071 phase Substances 0.000 claims 3
- 239000000872 buffer Substances 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 238000011534 incubation Methods 0.000 claims 2
- 102000009027 Albumins Human genes 0.000 claims 1
- 108010088751 Albumins Proteins 0.000 claims 1
- 108010052014 Liberase Proteins 0.000 claims 1
- 239000012062 aqueous buffer Substances 0.000 claims 1
- 238000005119 centrifugation Methods 0.000 claims 1
- 238000010790 dilution Methods 0.000 claims 1
- 239000012895 dilution Substances 0.000 claims 1
- 238000001914 filtration Methods 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
- 238000004448 titration Methods 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/73—Hydrolases (EC 3.)
- C12N2501/734—Proteases (EC 3.4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Developmental Biology & Embryology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Rheumatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Claims (12)
1. Postupak pripreme frakcija matičnih stanica porijeklom iz masnog tkiva, obuhvaća korake:
(a) uzimanje ili dobivanje uzorka masnog tkiva koji sadrži matične stanice;
(b) ispiranje uzorka iz koraka (a) prikladnim vodenim puferom;
(c) inkubacija uzorka iz koraka (b) pomoću enzima koji može razgraditi masno tkivo i iz njega ekstrahirati matične stanice;
(d) izdvajanje vodene faze iz proizvoda iz koraka (c);
(e) pročišćavanje vodene faze dobivene u koraku (d);
(f) titracija vodene faze dobivene u koraku (e) i, ako je potrebno, razrjeđivanje radi dobivanja konačne frakcije matičnih stanica željene koncentracije i volumena
gdje se materijal koji sadrži matične stanice obrađuje unutar štrcaljke barem kroz korake: (a), (b) i (c), ta štrcaljka ima funkcije sredstva za uzimanje, separatora faza i reaktora postupka.
2. Postupak prema zahtjevu 1, gdje štrcaljka ima volumen punjenja u rasponu od 20 do 100 ml.
3. Postupak prema zahtjevima 1-2, gdje štrcaljka ima jednu ili više oznaka koje pokazuju optimalni volumen(e) punjenja i/ili ima područja za pisanje ili označavanje.
4. Postupak prema zahtjevima 1-3, gdje je masni materijal lipoaspirat.
5. Postupak prema zahtjevima 1-4, gdje se koraci (a), odnosno (b-d) provode od strane dva različita operatera na međusobno udaljenim lokacijama.
6. Postupak prema zahtjevima 1-5, gdje u koraku (b) pufer je PBS pufer s dodacima enzimskih nutrijenata.
7. Postupak prema zahtjevima 1-6, gdje koraci (b) i/ili (c) obuhvaćaju okretanje štrcaljke prema dolje (igla prema dolje) ili prema gore (igla prema gore), nakon čega slijedi izbacivanje faze koja se nalazi uz iglu.
8. Postupak prema zahtjevima 1-7, gdje u koraku (c) enzim je liberaza, i inkubacija se provodi 20 do 80 minuta, na temperaturi od 30 do 45°C.
9. Postupak prema zahtjevima 1-8, gdje se u koraku (d) inkubacijska smjesa miješa s otopinom koja sadrži albumin, zatim se lipidna faza odbacuje i dobiva vodena faza za sljedeće korake postupka.
10. Postupak prema zahtjevima 1-9, gdje se materijal s matičnim stanicama dalje održava unutar štrcaljke tijekom jednog ili više od koraka (d)-(e).
11. Postupak prema zahtjevima 1-10, gdje se pročišćavanje u koraku (e) provodi centrifugiranjem(ima) i/ili filtracijom(ama).
12. Postupak prema zahtjevima 1-11, gdje se konačna frakcija matičnih stanica oblikuje kao jedna ili više jedinica od 1-5 ml, tako da je ukupna koncentracija nukleotidnih stanica između 108 do 104 stanica/ml.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2012/069261 WO2014048501A1 (en) | 2012-09-28 | 2012-09-28 | High-safety process for the preparation of purified stem cell fractions |
| EP12777875.1A EP2726602B2 (en) | 2012-09-28 | 2012-09-28 | High-safety process for the preparation of purified stem cell fractions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20141138T1 true HRP20141138T1 (hr) | 2015-01-30 |
Family
ID=47073416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20141138AT HRP20141138T1 (hr) | 2012-09-28 | 2014-11-24 | Postupak visoke sigurnosti za pripremu proäśišä†enih frakcija matiäśnih stanica |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US20150218521A1 (hr) |
| EP (1) | EP2726602B2 (hr) |
| JP (1) | JP6062055B2 (hr) |
| KR (1) | KR20150046274A (hr) |
| CN (1) | CN103842496B (hr) |
| BR (1) | BR112013028726A2 (hr) |
| CA (1) | CA2839800A1 (hr) |
| DK (1) | DK2726602T3 (hr) |
| ES (1) | ES2525273T3 (hr) |
| HK (1) | HK1197920A1 (hr) |
| HR (1) | HRP20141138T1 (hr) |
| IL (1) | IL237978A0 (hr) |
| RU (1) | RU2611201C2 (hr) |
| SM (1) | SMT201500018B (hr) |
| WO (1) | WO2014048501A1 (hr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107921182B (zh) * | 2015-06-10 | 2022-09-02 | 特拉维夫医学研究、基础设施和卫生服务医疗中心基金会 | 用于分离基质血管部分的机械仪器和方法 |
| DK3430122T3 (da) * | 2016-03-17 | 2021-08-23 | Synova Life Sciences Inc | Separering, adskillelse og/eller opdeling af celler ved hjælp af mekaniske stød |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6623959B2 (en) * | 2001-06-13 | 2003-09-23 | Ethicon, Inc. | Devices and methods for cell harvesting |
| EP2308963A3 (en) * | 2001-12-07 | 2011-09-21 | Cytori Therapeutics, Inc. | System for processing lipoaspirate cells |
| US20050048036A1 (en) * | 2001-12-07 | 2005-03-03 | Hedrick Marc H. | Methods of using regenerative cells in the treatment of inherited and acquired disorders of the bone, bone marrow, liver, and other tissues |
| PL375067A1 (en) * | 2002-07-19 | 2005-11-14 | Vesta Therapeutics, Inc. | Method of obtaining viable human liver cells, including hepatic stem/progenitor cells |
-
2012
- 2012-09-28 BR BR112013028726A patent/BR112013028726A2/pt not_active Application Discontinuation
- 2012-09-28 WO PCT/EP2012/069261 patent/WO2014048501A1/en active Application Filing
- 2012-09-28 DK DK12777875.1T patent/DK2726602T3/en active
- 2012-09-28 US US14/115,114 patent/US20150218521A1/en not_active Abandoned
- 2012-09-28 JP JP2015533461A patent/JP6062055B2/ja not_active Expired - Fee Related
- 2012-09-28 EP EP12777875.1A patent/EP2726602B2/en not_active Not-in-force
- 2012-09-28 RU RU2013143852A patent/RU2611201C2/ru not_active IP Right Cessation
- 2012-09-28 KR KR1020157007513A patent/KR20150046274A/ko not_active Application Discontinuation
- 2012-09-28 CA CA2839800A patent/CA2839800A1/en not_active Abandoned
- 2012-09-28 CN CN201280021056.4A patent/CN103842496B/zh not_active Expired - Fee Related
- 2012-09-28 ES ES12777875.1T patent/ES2525273T3/es active Active
-
2014
- 2014-11-03 HK HK14111001.8A patent/HK1197920A1/xx not_active IP Right Cessation
- 2014-11-24 HR HRP20141138AT patent/HRP20141138T1/hr unknown
-
2015
- 2015-01-21 SM SM201500018T patent/SMT201500018B/xx unknown
- 2015-03-26 IL IL237978A patent/IL237978A0/en unknown
-
2016
- 2016-09-28 US US15/278,152 patent/US20170015977A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ES2525273T3 (es) | 2014-12-19 |
| HK1197920A1 (en) | 2015-02-27 |
| EP2726602B1 (en) | 2014-10-22 |
| WO2014048501A1 (en) | 2014-04-03 |
| EP2726602A1 (en) | 2014-05-07 |
| IL237978A0 (en) | 2015-05-31 |
| BR112013028726A2 (pt) | 2017-01-24 |
| RU2013143852A (ru) | 2015-04-10 |
| SMT201500018B (it) | 2015-03-05 |
| US20150218521A1 (en) | 2015-08-06 |
| US20170015977A1 (en) | 2017-01-19 |
| CA2839800A1 (en) | 2014-03-28 |
| KR20150046274A (ko) | 2015-04-29 |
| JP2015530100A (ja) | 2015-10-15 |
| CN103842496B (zh) | 2017-05-17 |
| RU2611201C2 (ru) | 2017-02-21 |
| JP6062055B2 (ja) | 2017-01-18 |
| CN103842496A (zh) | 2014-06-04 |
| DK2726602T3 (en) | 2015-01-26 |
| EP2726602B2 (en) | 2018-05-30 |
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