HK1217726A1 - 寡核苷酸接頭及其在構建核酸測序單鏈環狀文庫中的應用 - Google Patents
寡核苷酸接頭及其在構建核酸測序單鏈環狀文庫中的應用Info
- Publication number
- HK1217726A1 HK1217726A1 HK16104236.8A HK16104236A HK1217726A1 HK 1217726 A1 HK1217726 A1 HK 1217726A1 HK 16104236 A HK16104236 A HK 16104236A HK 1217726 A1 HK1217726 A1 HK 1217726A1
- Authority
- HK
- Hong Kong
- Prior art keywords
- construction
- nucleic acid
- sequencing library
- circular nucleic
- stranded circular
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
- C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/00527—Sheets
- B01J2219/00529—DNA chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/537—Detection characterised by immobilisation to a surface characterised by the capture oligonucleotide acting as a primer
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Structural Engineering (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2014/086418 WO2016037358A1 (zh) | 2014-09-12 | 2014-09-12 | 分离的寡核苷酸及其在核酸测序中的用途 |
Publications (1)
Publication Number | Publication Date |
---|---|
HK1217726A1 true HK1217726A1 (zh) | 2017-01-20 |
Family
ID=55458278
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HK16103684.7A HK1215721A1 (zh) | 2014-09-12 | 2016-03-30 | 用於基於血液樣品構建測序文庫的方法及其在確定胎兒遺傳異常中的用途 |
HK16104236.8A HK1217726A1 (zh) | 2014-09-12 | 2016-04-13 | 寡核苷酸接頭及其在構建核酸測序單鏈環狀文庫中的應用 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HK16103684.7A HK1215721A1 (zh) | 2014-09-12 | 2016-03-30 | 用於基於血液樣品構建測序文庫的方法及其在確定胎兒遺傳異常中的用途 |
Country Status (9)
Country | Link |
---|---|
US (4) | US9890375B2 (zh) |
EP (5) | EP3192869B1 (zh) |
JP (3) | JP6483249B2 (zh) |
CN (3) | CN107075513B (zh) |
AU (3) | AU2014406026B2 (zh) |
DK (4) | DK3192869T3 (zh) |
ES (5) | ES2726149T3 (zh) |
HK (2) | HK1215721A1 (zh) |
WO (4) | WO2016037358A1 (zh) |
Families Citing this family (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3561072A1 (en) | 2012-12-10 | 2019-10-30 | Resolution Bioscience, Inc. | Methods for targeted genomic analysis |
US9328382B2 (en) | 2013-03-15 | 2016-05-03 | Complete Genomics, Inc. | Multiple tagging of individual long DNA fragments |
CN109971826B (zh) * | 2014-01-31 | 2022-12-30 | 斯威夫特生物科学股份有限公司 | 用于加工dna底物的改进方法 |
US10208338B2 (en) | 2014-03-03 | 2019-02-19 | Swift Biosciences, Inc. | Enhanced adaptor ligation |
CN106715713B (zh) * | 2014-09-12 | 2020-11-03 | 深圳华大智造科技有限公司 | 试剂盒及其在核酸测序中的用途 |
US20180080092A1 (en) * | 2014-10-14 | 2018-03-22 | Bgi Shenzhen Co., Limited | One-stop treatment method for breaking nucleic acid by means of transposase, and reagent |
US10221448B2 (en) | 2015-03-06 | 2019-03-05 | Pillar Biosciences Inc. | Selective amplification of overlapping amplicons |
ES2856598T3 (es) | 2015-11-11 | 2021-09-27 | Resolution Bioscience Inc | Construcción de alta eficiencia de bibliotecas de ADN |
US11299780B2 (en) | 2016-07-15 | 2022-04-12 | The Regents Of The University Of California | Methods of producing nucleic acid libraries |
WO2018015365A1 (en) * | 2016-07-18 | 2018-01-25 | Roche Sequencing Solutions, Inc. | Asymmetric templates and asymmetric method of nucleic acid sequencing |
AU2017312953A1 (en) | 2016-08-16 | 2019-01-24 | The Regents Of The University Of California | Method for finding low abundance sequences by hybridization (flash) |
RU2019108294A (ru) | 2016-08-25 | 2020-09-25 | Резолюшн Байосайенс, Инк. | Способы обнаружения изменений количества геномных копий в образцах днк |
WO2018049049A1 (en) * | 2016-09-07 | 2018-03-15 | Baylor College Of Medicine | Clinical application of cell free dna technologies to non-invasive prenatal diagnosis and other liquid biopsies |
WO2018090373A1 (zh) * | 2016-11-21 | 2018-05-24 | 深圳华大智造科技有限公司 | 一种dna末端修复与加a的方法 |
JP6847499B2 (ja) * | 2016-12-20 | 2021-03-24 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | 環状コンセンサスシークエンシングのための一本鎖環状dnaライブラリー |
DK3452621T3 (da) * | 2017-02-21 | 2022-12-12 | Illumina Inc | Tagmentation ved brug af immobiliserede transposomer med linkere |
CN111315895A (zh) * | 2017-09-14 | 2020-06-19 | 豪夫迈·罗氏有限公司 | 用于产生环状单链dna文库的新型方法 |
CN109750086B (zh) * | 2017-11-06 | 2022-08-02 | 深圳华大智造科技股份有限公司 | 单链环状文库的构建方法 |
WO2019140201A1 (en) | 2018-01-12 | 2019-07-18 | Claret Bioscience, Llc | Methods and compositions for analyzing nucleic acid |
JP7096893B2 (ja) * | 2018-02-05 | 2022-07-06 | エフ.ホフマン-ラ ロシュ アーゲー | 単一分子のための一本鎖環状dna鋳型の作製 |
CN112639094A (zh) * | 2018-05-08 | 2021-04-09 | 深圳华大智造科技股份有限公司 | 用于准确且经济高效的测序、单体型分型和组装的基于单管珠粒的dna共条形码化 |
US11629345B2 (en) | 2018-06-06 | 2023-04-18 | The Regents Of The University Of California | Methods of producing nucleic acid libraries and compositions and kits for practicing same |
US12049665B2 (en) | 2018-06-12 | 2024-07-30 | Accuragen Holdings Limited | Methods and compositions for forming ligation products |
CN109023536A (zh) * | 2018-06-28 | 2018-12-18 | 河南师范大学 | 一种植物降解组文库构建方法 |
CN110734967B (zh) * | 2018-07-19 | 2023-02-17 | 深圳华大智造科技股份有限公司 | 一种接头组合物及其应用 |
WO2020157684A1 (en) | 2019-01-29 | 2020-08-06 | Mgi Tech Co., Ltd. | High coverage stlfr |
CN112342627B (zh) * | 2019-08-09 | 2024-07-23 | 深圳市真迈生物科技有限公司 | 一种核酸文库的制备方法及测序方法 |
CN111139533B (zh) * | 2019-09-27 | 2021-11-09 | 上海英基生物科技有限公司 | 稳定性增加的测序文库接头 |
RU2746960C1 (ru) * | 2020-09-23 | 2021-04-22 | Федеральное государственное автономное образовательное учреждение высшего образования "Российский национальный исследовательский медицинский университет имени Н.И. Пирогова" Министерства здравоохранения Российской Федерации (ФГАОУ ВО РНИМУ им. Н.И. Пирогова Минздрава России) | Способ создания кольцевой формы NGS библиотеки для высокопроизводительного секвенирования на платформах технологии DNBSEQ |
CN116042767A (zh) * | 2021-10-28 | 2023-05-02 | 深圳华大生命科学研究院 | 测序文库的构建方法及试剂盒 |
CN113943764B (zh) * | 2021-12-20 | 2022-03-04 | 中国海洋大学 | 一种制备双链rna的方法 |
WO2023240611A1 (zh) * | 2022-06-17 | 2023-12-21 | 深圳华大智造科技股份有限公司 | 单链核酸环状文库的建库以及测序方法 |
Family Cites Families (47)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0923650B1 (en) * | 1996-06-06 | 2007-03-07 | Solexa, Inc | Sequencing by ligation of encoded adaptors |
WO2001040516A2 (en) | 1999-12-02 | 2001-06-07 | Molecular Staging Inc. | Generation of single-strand circular dna from linear self-annealing segments |
DE602004036672C5 (de) * | 2003-01-29 | 2012-11-29 | 454 Life Sciences Corporation | Nukleinsäureamplifikation auf Basis von Kügelchenemulsion |
US20080318215A1 (en) | 2005-12-20 | 2008-12-25 | Ming-Sheng Lee | Apparatus, methods and products for detecting genetic mutation |
US20070172839A1 (en) * | 2006-01-24 | 2007-07-26 | Smith Douglas R | Asymmetrical adapters and methods of use thereof |
US8679741B2 (en) | 2006-05-31 | 2014-03-25 | Sequenom, Inc. | Methods and compositions for the extraction and amplification of nucleic acid from a sample |
US9328378B2 (en) * | 2006-07-31 | 2016-05-03 | Illumina Cambridge Limited | Method of library preparation avoiding the formation of adaptor dimers |
US7910354B2 (en) * | 2006-10-27 | 2011-03-22 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
US20090111706A1 (en) | 2006-11-09 | 2009-04-30 | Complete Genomics, Inc. | Selection of dna adaptor orientation by amplification |
US8518640B2 (en) * | 2007-10-29 | 2013-08-27 | Complete Genomics, Inc. | Nucleic acid sequencing and process |
US7901890B2 (en) | 2007-11-05 | 2011-03-08 | Complete Genomics, Inc. | Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation |
CA2707901C (en) * | 2007-12-05 | 2015-09-15 | Complete Genomics, Inc. | Efficient base determination in sequencing reactions |
EP2268834B1 (en) * | 2008-03-17 | 2015-01-07 | Stichting Genetwister IP | Expression-linked gene discovery |
AU2009229157B2 (en) * | 2008-03-28 | 2015-01-29 | Pacific Biosciences Of California, Inc. | Compositions and methods for nucleic acid sequencing |
WO2009133466A2 (en) * | 2008-04-30 | 2009-11-05 | Population Genetics Technologies Ltd. | Asymmetric adapter library construction |
CN102203273A (zh) * | 2008-09-09 | 2011-09-28 | 生命技术公司 | 生成基因特异性的文库的方法 |
US8383345B2 (en) * | 2008-09-12 | 2013-02-26 | University Of Washington | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
EP2508529B1 (en) * | 2008-10-24 | 2013-08-28 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
US9080211B2 (en) * | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
EP2432899A1 (en) * | 2009-05-22 | 2012-03-28 | Population Genetics Technologies LTD. | Sorting asymmetrically tagged nucleic acids by selective primer extension |
US9023769B2 (en) * | 2009-11-30 | 2015-05-05 | Complete Genomics, Inc. | cDNA library for nucleic acid sequencing |
AU2010330936B2 (en) * | 2009-12-17 | 2014-05-22 | Keygene N.V. | Restriction enzyme based whole genome sequencing |
EP2545183B1 (en) * | 2010-03-10 | 2017-04-19 | Ibis Biosciences, Inc. | Production of single-stranded circular nucleic acid |
WO2011161549A2 (en) * | 2010-06-24 | 2011-12-29 | Population Genetics Technologies Ltd. | Methods and compositions for polynucleotide library production, immortalization and region of interest extraction |
JP5968879B2 (ja) * | 2010-06-30 | 2016-08-10 | 深▲せん▼華大基因股▲ふん▼有限公司 | Dna分子タグ技術及びdna不完全断片化技術に基づいたpcrシークエンシング法及びそれを用いたhla遺伝子タイピング法 |
CN102409049B (zh) | 2010-09-21 | 2013-10-23 | 深圳华大基因科技服务有限公司 | 一种基于pcr的dna标签文库构建方法 |
CN102409045B (zh) | 2010-09-21 | 2013-09-18 | 深圳华大基因科技服务有限公司 | 一种基于dna接头连接的标签文库构建方法及其所使用标签和标签接头 |
CN102534811B (zh) | 2010-12-16 | 2013-11-20 | 深圳华大基因科技服务有限公司 | 一种dna文库及其制备方法、一种dna测序方法和装置 |
CN102181943B (zh) * | 2011-03-02 | 2013-06-05 | 中山大学 | 一种配对双末端文库构建方法及用该文库进行基因组测序的方法 |
EP2710172B1 (en) * | 2011-05-20 | 2017-03-29 | Fluidigm Corporation | Nucleic acid encoding reactions |
CN102296065B (zh) * | 2011-08-04 | 2013-05-15 | 盛司潼 | 用于构建测序文库的系统与方法 |
CN103014137B (zh) * | 2011-09-22 | 2015-01-07 | 深圳华大基因科技服务有限公司 | 一种分析基因表达定量的方法 |
CA2852949A1 (en) * | 2011-10-19 | 2013-04-25 | Nugen Technologies, Inc. | Compositions and methods for directional nucleic acid amplification and sequencing |
CN103103624B (zh) * | 2011-11-15 | 2014-12-31 | 深圳华大基因科技服务有限公司 | 高通量测序文库的构建方法及其应用 |
EP2807292B1 (en) * | 2012-01-26 | 2019-05-22 | Tecan Genomics, Inc. | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation |
NO2694769T3 (zh) * | 2012-03-06 | 2018-03-03 | ||
ES2905448T3 (es) * | 2012-05-10 | 2022-04-08 | Massachusetts Gen Hospital | Métodos para determinar una secuencia nucleotídica |
CN102703426A (zh) * | 2012-05-21 | 2012-10-03 | 吴江汇杰生物科技有限公司 | 构建核酸库的方法、试剂及试剂盒 |
GB2518078B (en) * | 2012-06-18 | 2015-04-29 | Nugen Technologies Inc | Compositions and methods for negative selection of non-desired nucleic acid sequences |
CN102703432A (zh) * | 2012-07-11 | 2012-10-03 | 烟台博诺生物科技有限公司 | 构建核酸库的方法、试剂及试剂盒 |
US9644199B2 (en) | 2012-10-01 | 2017-05-09 | Agilent Technologies, Inc. | Immobilized transposase complexes for DNA fragmentation and tagging |
WO2014086037A1 (zh) * | 2012-12-07 | 2014-06-12 | 深圳华大基因科技服务有限公司 | 构建核酸测序文库的方法及其应用 |
US9683230B2 (en) * | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
CN103667273B (zh) * | 2013-12-05 | 2016-01-20 | 北京诺禾致源生物信息科技有限公司 | 双链接头、其应用及构建末端配对dna文库的方法 |
CN109971826B (zh) | 2014-01-31 | 2022-12-30 | 斯威夫特生物科学股份有限公司 | 用于加工dna底物的改进方法 |
WO2016078096A1 (zh) * | 2014-11-21 | 2016-05-26 | 深圳华大基因科技有限公司 | 使用鼓泡状接头元件构建测序文库的方法 |
CN107124888B (zh) | 2014-11-21 | 2021-08-06 | 深圳华大智造科技股份有限公司 | 鼓泡状接头元件和使用其构建测序文库的方法 |
-
2014
- 2014-09-12 AU AU2014406026A patent/AU2014406026B2/en active Active
- 2014-09-12 US US15/510,877 patent/US9890375B2/en active Active
- 2014-09-12 ES ES14901591T patent/ES2726149T3/es active Active
- 2014-09-12 CN CN201480081861.5A patent/CN107075513B/zh active Active
- 2014-09-12 WO PCT/CN2014/086418 patent/WO2016037358A1/zh active Application Filing
- 2014-09-12 DK DK14901591.9T patent/DK3192869T3/da active
- 2014-09-12 EP EP14901591.9A patent/EP3192869B1/en active Active
- 2014-09-12 JP JP2017514336A patent/JP6483249B2/ja active Active
- 2014-09-26 ES ES14901739.4T patent/ES2682068T3/es active Active
- 2014-09-26 WO PCT/CN2014/087589 patent/WO2016037389A1/en active Application Filing
- 2014-09-26 EP EP14901739.4A patent/EP3191630B1/en active Active
- 2014-10-14 WO PCT/CN2014/088543 patent/WO2016037394A1/zh active Application Filing
- 2014-10-14 US US15/510,904 patent/US10023906B2/en active Active
- 2014-10-14 AU AU2014405969A patent/AU2014405969B2/en active Active
- 2014-10-14 EP EP14901597.6A patent/EP3192900B1/en active Active
- 2014-10-14 DK DK14901597.6T patent/DK3192900T3/en active
- 2014-10-14 ES ES14901597.6T patent/ES2689353T3/es active Active
- 2014-10-14 JP JP2017514335A patent/JP6438126B2/ja active Active
- 2014-10-14 CN CN201480081852.6A patent/CN107075731B/zh active Active
- 2014-11-21 US US15/510,890 patent/US10544451B2/en active Active
- 2014-11-21 JP JP2017513705A patent/JP6679576B2/ja active Active
- 2014-11-21 WO PCT/CN2014/091852 patent/WO2016037416A1/zh active Application Filing
- 2014-11-21 EP EP14901593.5A patent/EP3192877B1/en active Active
- 2014-11-21 ES ES14901593T patent/ES2708804T3/es active Active
- 2014-11-21 DK DK18174793.2T patent/DK3388519T3/da active
- 2014-11-21 ES ES18174793T patent/ES2738480T3/es active Active
- 2014-11-21 EP EP18174793.2A patent/EP3388519B1/en active Active
- 2014-11-21 AU AU2014405991A patent/AU2014405991B2/en active Active
- 2014-11-21 CN CN201480081687.4A patent/CN106795514B/zh active Active
- 2014-11-21 DK DK14901593.5T patent/DK3192877T3/en active
-
2016
- 2016-03-30 HK HK16103684.7A patent/HK1215721A1/zh unknown
- 2016-04-13 HK HK16104236.8A patent/HK1217726A1/zh unknown
-
2019
- 2019-09-19 US US16/576,582 patent/US10995367B2/en active Active
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
HK1217726A1 (zh) | 寡核苷酸接頭及其在構建核酸測序單鏈環狀文庫中的應用 | |
IL288263A (en) | CRISPR DNA/RNA hybrid polynucleotides and methods for using them | |
HK1250051A1 (zh) | 核酸測序方法和系統 | |
HK1219503A1 (zh) | 用於核酸測序的方法和組合物 | |
HK1217734A1 (zh) | 包含基因組 兩者的總核酸的富集和新代測序 | |
SG11201605906UA (en) | Muscle-specific nucleic acid regulatory elements and methods and use thereof | |
HK1218318A1 (zh) | 構建可變區測序文庫的方法及確定可變區核酸序列的方法 | |
HK1250758A1 (zh) | 一種核酸單鏈環狀文庫的構建方法和試劑 | |
IL251390B (en) | Polysaccharide and nucleic acid formulations that include viscosity-reducing agents | |
BR112017008877A2 (pt) | métodos e composições para sequenciamento de ácido nucleico-alvo | |
HK1206072A1 (zh) | 核酸測序的改進方法 | |
EP3194627C0 (en) | METHODS AND SYSTEMS FOR ANALYZING NUCLEIC ACID SEQUENCING DATA | |
HK1221227A1 (zh) | 用於基於納米結構的核酸測序的方法和組合物 | |
EP3237613A4 (en) | Visualizing modified nucleotides and nucleic acid interactions in single cells | |
GB201518786D0 (en) | Molecular and bioinformatics methods for direct sequencing | |
GB201405919D0 (en) | A combination product comprising a nucleic acid structure and a single-stranded nucleic acid molecule | |
EP3294309A4 (en) | NUCLEIC ACID MOLECULES WITH SPACERS AND METHOD FOR USE THEREOF | |
EP2844767A4 (en) | SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING | |
EP3361869A4 (en) | NUCLEIC ACID MOLECULES WITH SPACERS AND METHOD FOR USE THEREOF | |
HK1217342A1 (zh) | 對轉錄激活子樣效應因子核酸酶及其編碼序列與應用 | |
GB2530829B (en) | Mutated genes for the catalytic protein of oplophorus luciferase and the use thereof | |
GB201411358D0 (en) | Selective oligonucleotide probes and primers | |
GB201509395D0 (en) | Improvements in and relating to nucleic acid probes | |
GB201310080D0 (en) | Selective oligonucleotide probes and primers |