CN102534811B - 一种dna文库及其制备方法、一种dna测序方法和装置 - Google Patents
一种dna文库及其制备方法、一种dna测序方法和装置 Download PDFInfo
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- CN102534811B CN102534811B CN2010105914487A CN201010591448A CN102534811B CN 102534811 B CN102534811 B CN 102534811B CN 2010105914487 A CN2010105914487 A CN 2010105914487A CN 201010591448 A CN201010591448 A CN 201010591448A CN 102534811 B CN102534811 B CN 102534811B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2010105914487A CN102534811B (zh) | 2010-12-16 | 2010-12-16 | 一种dna文库及其制备方法、一种dna测序方法和装置 |
PCT/CN2011/083726 WO2012079486A1 (zh) | 2010-12-16 | 2011-12-08 | 制备用于测序的dna样品的方法及其应用 |
HK12110105.7A HK1169460A1 (en) | 2010-12-16 | 2012-10-12 | Dna library and its construction method, dna sequencing method and apparatus thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN2010105914487A CN102534811B (zh) | 2010-12-16 | 2010-12-16 | 一种dna文库及其制备方法、一种dna测序方法和装置 |
Publications (2)
Publication Number | Publication Date |
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CN102534811A CN102534811A (zh) | 2012-07-04 |
CN102534811B true CN102534811B (zh) | 2013-11-20 |
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CN2010105914487A Active CN102534811B (zh) | 2010-12-16 | 2010-12-16 | 一种dna文库及其制备方法、一种dna测序方法和装置 |
Country Status (3)
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CN (1) | CN102534811B (zh) |
HK (1) | HK1169460A1 (zh) |
WO (1) | WO2012079486A1 (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104153003A (zh) * | 2014-08-08 | 2014-11-19 | 上海美吉生物医药科技有限公司 | 一种基于illumina测序平台的大片段DNA文库的构建方法 |
CN107794573A (zh) * | 2016-08-31 | 2018-03-13 | 安诺优达基因科技(北京)有限公司 | 一种构建dna大片段文库的方法及其应用 |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102839168A (zh) * | 2012-07-31 | 2012-12-26 | 深圳华大基因研究院 | 核酸探针及其制备方法和应用 |
CN103627710B (zh) * | 2012-08-22 | 2016-08-03 | 中国人民解放军总医院 | Spg11基因突变体及其应用 |
CN103290104B (zh) * | 2013-01-23 | 2016-03-02 | 北京诺禾致源生物信息科技有限公司 | 一种应用于第二代测序的简捷廉价的基因组样品破碎方法 |
US10023856B2 (en) * | 2013-09-25 | 2018-07-17 | Thermo Fisher Scientific Baltics Uab | Enzyme composition for DNA end repair, adenylation, phosphorylation |
CN103849617A (zh) * | 2013-12-16 | 2014-06-11 | 复旦大学 | 用于基因组dna永久保存的接头及方法 |
CN104109709A (zh) * | 2014-04-04 | 2014-10-22 | 北京泛生子生物科技有限公司 | 用于癌症个体化诊断治疗的重要基因富集方法 |
US9890375B2 (en) | 2014-09-12 | 2018-02-13 | Bgi Shenzhen Co., Limited | Isolated oligonucleotide and use thereof in nucleic acid sequencing |
WO2016049929A1 (zh) * | 2014-09-30 | 2016-04-07 | 天津华大基因科技有限公司 | 构建测序文库的方法及其应用 |
US20170349893A1 (en) * | 2014-11-26 | 2017-12-07 | Bgi Shenzhen | Method and reagent for constructing nucleic acid double-linker single-strand cyclical library |
US10479991B2 (en) | 2014-11-26 | 2019-11-19 | Mgi Tech Co., Ltd | Method and reagent for constructing nucleic acid double-linker single-strand cyclical library |
CN105986324B (zh) * | 2015-02-11 | 2018-08-14 | 深圳华大智造科技有限公司 | 环状小rna文库构建方法及其应用 |
CN106319639B (zh) * | 2015-06-17 | 2018-09-04 | 深圳华大智造科技有限公司 | 构建测序文库的方法及设备 |
CN105002570B (zh) * | 2015-07-21 | 2017-09-05 | 中国农业科学院深圳农业基因组研究所 | 一种一次制备多个dna大片段插入双末端测序文库的方法 |
CN106554957B (zh) * | 2015-09-30 | 2020-04-21 | 中国农业科学院深圳农业基因组研究所 | 测序文库及其制备和应用 |
CN107794572B (zh) * | 2016-08-31 | 2022-04-05 | 安诺优达基因科技(北京)有限公司 | 一种构建大片段文库的方法及其应用 |
CN108342385A (zh) * | 2017-01-22 | 2018-07-31 | 中国科学院天津工业生物技术研究所 | 一种接头和通过高效率环化方式构建测序文库的方法 |
CN108866154B (zh) * | 2017-05-15 | 2021-11-16 | 深圳华大基因股份有限公司 | 基于长片段dna捕获和三代测序的无创产前单体型构建方法 |
CN108866172B (zh) * | 2017-05-15 | 2021-11-16 | 深圳华大基因股份有限公司 | 基于长片段dna环化和三代测序的无创产前单体型构建方法 |
CN109988817B (zh) * | 2017-12-30 | 2020-12-04 | 安诺优达基因科技(北京)有限公司 | 一种随机打断dna的方法 |
CN109266718A (zh) * | 2018-10-19 | 2019-01-25 | 广东菲鹏生物有限公司 | 检测核酸内切酶存在的方法 |
CN109868270B (zh) * | 2019-03-11 | 2021-02-26 | 深圳乐土生物科技有限公司 | 低起始量的dna文库构建方法 |
CN112176028B (zh) * | 2019-07-05 | 2024-04-16 | 深圳华大生命科学研究院 | 一种基于核酸内切酶的快速wgs建库方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69932345T2 (de) * | 1998-10-26 | 2007-07-19 | Novozymes A/S | Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen |
EP1539951A1 (en) * | 2002-06-21 | 2005-06-15 | Sinogenomax Co., Ltd. | Randomised dna libraries and double-stranded rna libraries, use and method of production thereof |
CN101351552A (zh) * | 2005-06-06 | 2009-01-21 | 454生命科学公司 | 配对末端测序 |
EP1999276A4 (en) * | 2006-03-14 | 2010-08-04 | Genizon Biosciences Inc | METHOD AND AGENT FOR NUCLEIC ACID SEQUENCING |
-
2010
- 2010-12-16 CN CN2010105914487A patent/CN102534811B/zh active Active
-
2011
- 2011-12-08 WO PCT/CN2011/083726 patent/WO2012079486A1/zh active Application Filing
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2012
- 2012-10-12 HK HK12110105.7A patent/HK1169460A1/xx unknown
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104153003A (zh) * | 2014-08-08 | 2014-11-19 | 上海美吉生物医药科技有限公司 | 一种基于illumina测序平台的大片段DNA文库的构建方法 |
CN107794573A (zh) * | 2016-08-31 | 2018-03-13 | 安诺优达基因科技(北京)有限公司 | 一种构建dna大片段文库的方法及其应用 |
CN107794573B (zh) * | 2016-08-31 | 2022-09-13 | 浙江安诺优达生物科技有限公司 | 一种构建dna大片段文库的方法及其应用 |
Also Published As
Publication number | Publication date |
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WO2012079486A1 (zh) | 2012-06-21 |
CN102534811A (zh) | 2012-07-04 |
HK1169460A1 (en) | 2013-01-25 |
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