HK1169460A1 - Dna library and its construction method, dna sequencing method and apparatus thereof - Google Patents
Dna library and its construction method, dna sequencing method and apparatus thereofInfo
- Publication number
- HK1169460A1 HK1169460A1 HK12110105.7A HK12110105A HK1169460A1 HK 1169460 A1 HK1169460 A1 HK 1169460A1 HK 12110105 A HK12110105 A HK 12110105A HK 1169460 A1 HK1169460 A1 HK 1169460A1
- Authority
- HK
- Hong Kong
- Prior art keywords
- dna
- construction method
- dna sequencing
- library
- dna library
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2010105914487A CN102534811B (en) | 2010-12-16 | 2010-12-16 | DNA (deoxyribonucleic acid) library and preparation method thereof, as well as DNA sequencing method and device |
Publications (1)
Publication Number | Publication Date |
---|---|
HK1169460A1 true HK1169460A1 (en) | 2013-01-25 |
Family
ID=46244096
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HK12110105.7A HK1169460A1 (en) | 2010-12-16 | 2012-10-12 | Dna library and its construction method, dna sequencing method and apparatus thereof |
Country Status (3)
Country | Link |
---|---|
CN (1) | CN102534811B (en) |
HK (1) | HK1169460A1 (en) |
WO (1) | WO2012079486A1 (en) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102839168A (en) * | 2012-07-31 | 2012-12-26 | 深圳华大基因研究院 | Nucleic acid probe, and preparation method and application thereof |
CN103627710B (en) * | 2012-08-22 | 2016-08-03 | 中国人民解放军总医院 | SPG11 gene mutation body and application thereof |
CN103290104B (en) * | 2013-01-23 | 2016-03-02 | 北京诺禾致源生物信息科技有限公司 | A kind of genomic samples breaking method being applied to the simple and direct cheapness of s-generation order-checking |
US10023856B2 (en) * | 2013-09-25 | 2018-07-17 | Thermo Fisher Scientific Baltics Uab | Enzyme composition for DNA end repair, adenylation, phosphorylation |
CN103849617A (en) * | 2013-12-16 | 2014-06-11 | 复旦大学 | Connector and method for Permanent preservation of genome DNA (deoxyribonucleic acid) |
CN104109709A (en) * | 2014-04-04 | 2014-10-22 | 北京泛生子生物科技有限公司 | Important gene enrichment method used for individual cancer diagnosis and treatment |
CN104153003A (en) * | 2014-08-08 | 2014-11-19 | 上海美吉生物医药科技有限公司 | Method for establishing DNA (Deoxyribose Nucleic Acid) library based on illumina sequencing platform |
EP3192869B1 (en) | 2014-09-12 | 2019-03-27 | MGI Tech Co., Ltd. | Isolated oligonucleotide and use thereof in nucleic acid sequencing |
WO2016049929A1 (en) * | 2014-09-30 | 2016-04-07 | 天津华大基因科技有限公司 | Method for constructing sequencing library and application thereof |
US20170349893A1 (en) * | 2014-11-26 | 2017-12-07 | Bgi Shenzhen | Method and reagent for constructing nucleic acid double-linker single-strand cyclical library |
CN107002292B (en) * | 2014-11-26 | 2019-03-26 | 深圳华大智造科技有限公司 | A kind of construction method and reagent in the twin adapter single stranded circle library of nucleic acid |
CN105986324B (en) * | 2015-02-11 | 2018-08-14 | 深圳华大智造科技有限公司 | Cyclic annular tiny RNA library constructing method and its application |
CN106319639B (en) * | 2015-06-17 | 2018-09-04 | 深圳华大智造科技有限公司 | Build the method and apparatus of sequencing library |
CN105002570B (en) * | 2015-07-21 | 2017-09-05 | 中国农业科学院深圳农业基因组研究所 | A kind of method for once preparing multiple double end sequencing libraries of DNA large fragments insertion |
CN106554957B (en) * | 2015-09-30 | 2020-04-21 | 中国农业科学院深圳农业基因组研究所 | Sequencing library, preparation and application thereof |
CN107794573B (en) * | 2016-08-31 | 2022-09-13 | 浙江安诺优达生物科技有限公司 | Method for constructing DNA large fragment library and application thereof |
CN107794572B (en) * | 2016-08-31 | 2022-04-05 | 安诺优达基因科技(北京)有限公司 | Method for constructing large fragment library and application thereof |
CN108342385A (en) * | 2017-01-22 | 2018-07-31 | 中国科学院天津工业生物技术研究所 | A kind of connector and the method that sequencing library is built by way of high efficiency cyclisation |
CN108866172B (en) * | 2017-05-15 | 2021-11-16 | 深圳华大基因股份有限公司 | Noninvasive prenatal haplotype construction method based on long-fragment DNA cyclization and third-generation sequencing |
CN108866154B (en) * | 2017-05-15 | 2021-11-16 | 深圳华大基因股份有限公司 | Noninvasive prenatal haplotype construction method based on long-fragment DNA capture and third-generation sequencing |
CN112375807B (en) * | 2017-12-30 | 2023-02-21 | 浙江安诺优达生物科技有限公司 | Method for randomly breaking DNA |
CN109266718A (en) * | 2018-10-19 | 2019-01-25 | 广东菲鹏生物有限公司 | Detect method existing for endonuclease |
CN109868270B (en) * | 2019-03-11 | 2021-02-26 | 深圳乐土生物科技有限公司 | Low initial amount DNA library construction method |
CN112176028B (en) * | 2019-07-05 | 2024-04-16 | 深圳华大生命科学研究院 | Rapid WGS library construction method based on endonuclease |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE332968T1 (en) * | 1998-10-26 | 2006-08-15 | Novozymes As | CREATION AND SCREENING OF DNA BANKS OF INTEREST IN CELLS OF FILAMENTOUS FUNGI |
EP1539951A1 (en) * | 2002-06-21 | 2005-06-15 | Sinogenomax Co., Ltd. | Randomised dna libraries and double-stranded rna libraries, use and method of production thereof |
CN101351552A (en) * | 2005-06-06 | 2009-01-21 | 454生命科学公司 | Paired end sequencing |
CA2647786A1 (en) * | 2006-03-14 | 2007-09-20 | Genizon Biosciences Inc. | Methods and means for nucleic acid sequencing |
-
2010
- 2010-12-16 CN CN2010105914487A patent/CN102534811B/en active Active
-
2011
- 2011-12-08 WO PCT/CN2011/083726 patent/WO2012079486A1/en active Application Filing
-
2012
- 2012-10-12 HK HK12110105.7A patent/HK1169460A1/en unknown
Also Published As
Publication number | Publication date |
---|---|
CN102534811B (en) | 2013-11-20 |
CN102534811A (en) | 2012-07-04 |
WO2012079486A1 (en) | 2012-06-21 |
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