HK1169460A1 - Dna library and its construction method, dna sequencing method and apparatus thereof - Google Patents

Dna library and its construction method, dna sequencing method and apparatus thereof

Info

Publication number
HK1169460A1
HK1169460A1 HK12110105.7A HK12110105A HK1169460A1 HK 1169460 A1 HK1169460 A1 HK 1169460A1 HK 12110105 A HK12110105 A HK 12110105A HK 1169460 A1 HK1169460 A1 HK 1169460A1
Authority
HK
Hong Kong
Prior art keywords
dna
construction method
dna sequencing
library
dna library
Prior art date
Application number
HK12110105.7A
Other languages
English (en)
Original Assignee
Bgi Tech Solutions Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bgi Tech Solutions Co Ltd filed Critical Bgi Tech Solutions Co Ltd
Publication of HK1169460A1 publication Critical patent/HK1169460A1/xx

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK12110105.7A 2010-12-16 2012-10-12 Dna library and its construction method, dna sequencing method and apparatus thereof HK1169460A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2010105914487A CN102534811B (zh) 2010-12-16 2010-12-16 一种dna文库及其制备方法、一种dna测序方法和装置

Publications (1)

Publication Number Publication Date
HK1169460A1 true HK1169460A1 (en) 2013-01-25

Family

ID=46244096

Family Applications (1)

Application Number Title Priority Date Filing Date
HK12110105.7A HK1169460A1 (en) 2010-12-16 2012-10-12 Dna library and its construction method, dna sequencing method and apparatus thereof

Country Status (3)

Country Link
CN (1) CN102534811B (zh)
HK (1) HK1169460A1 (zh)
WO (1) WO2012079486A1 (zh)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102839168A (zh) * 2012-07-31 2012-12-26 深圳华大基因研究院 核酸探针及其制备方法和应用
CN103627710B (zh) * 2012-08-22 2016-08-03 中国人民解放军总医院 Spg11基因突变体及其应用
CN103290104B (zh) * 2013-01-23 2016-03-02 北京诺禾致源生物信息科技有限公司 一种应用于第二代测序的简捷廉价的基因组样品破碎方法
US10023856B2 (en) * 2013-09-25 2018-07-17 Thermo Fisher Scientific Baltics Uab Enzyme composition for DNA end repair, adenylation, phosphorylation
CN103849617A (zh) * 2013-12-16 2014-06-11 复旦大学 用于基因组dna永久保存的接头及方法
CN104109709A (zh) * 2014-04-04 2014-10-22 北京泛生子生物科技有限公司 用于癌症个体化诊断治疗的重要基因富集方法
CN104153003A (zh) * 2014-08-08 2014-11-19 上海美吉生物医药科技有限公司 一种基于illumina测序平台的大片段DNA文库的构建方法
AU2014406026B2 (en) * 2014-09-12 2018-08-23 Mgi Tech Co., Ltd. Isolated oligonucleotide and use thereof in nucleic acid sequencing
WO2016049929A1 (zh) * 2014-09-30 2016-04-07 天津华大基因科技有限公司 构建测序文库的方法及其应用
WO2016082129A1 (zh) * 2014-11-26 2016-06-02 深圳华大基因研究院 一种核酸的双接头单链环状文库的构建方法和试剂
CN107002291B (zh) * 2014-11-26 2019-03-26 深圳华大智造科技有限公司 一种核酸的双接头单链环状文库的构建方法和试剂
CN105986324B (zh) * 2015-02-11 2018-08-14 深圳华大智造科技有限公司 环状小rna文库构建方法及其应用
CN106319639B (zh) * 2015-06-17 2018-09-04 深圳华大智造科技有限公司 构建测序文库的方法及设备
CN105002570B (zh) * 2015-07-21 2017-09-05 中国农业科学院深圳农业基因组研究所 一种一次制备多个dna大片段插入双末端测序文库的方法
CN106554957B (zh) 2015-09-30 2020-04-21 中国农业科学院深圳农业基因组研究所 测序文库及其制备和应用
CN107794573B (zh) * 2016-08-31 2022-09-13 浙江安诺优达生物科技有限公司 一种构建dna大片段文库的方法及其应用
CN107794572B (zh) * 2016-08-31 2022-04-05 安诺优达基因科技(北京)有限公司 一种构建大片段文库的方法及其应用
CN108342385A (zh) * 2017-01-22 2018-07-31 中国科学院天津工业生物技术研究所 一种接头和通过高效率环化方式构建测序文库的方法
CN108866154B (zh) * 2017-05-15 2021-11-16 深圳华大基因股份有限公司 基于长片段dna捕获和三代测序的无创产前单体型构建方法
CN108866172B (zh) * 2017-05-15 2021-11-16 深圳华大基因股份有限公司 基于长片段dna环化和三代测序的无创产前单体型构建方法
CN109988817B (zh) * 2017-12-30 2020-12-04 安诺优达基因科技(北京)有限公司 一种随机打断dna的方法
CN109266718A (zh) * 2018-10-19 2019-01-25 广东菲鹏生物有限公司 检测核酸内切酶存在的方法
CN109868270B (zh) * 2019-03-11 2021-02-26 深圳乐土生物科技有限公司 低起始量的dna文库构建方法
CN112176028B (zh) * 2019-07-05 2024-04-16 深圳华大生命科学研究院 一种基于核酸内切酶的快速wgs建库方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1197965C (zh) * 1998-10-26 2005-04-20 诺维信公司 在丝状真菌细胞内构建和筛选目的dna文库
JP2005529624A (ja) * 2002-06-21 2005-10-06 シノゲノマックス カンパニー リミテッド ランダムｄｎａライブラリーと二本鎖ｒｎａライブラリー、その用途および生産方法
CN101351552A (zh) * 2005-06-06 2009-01-21 454生命科学公司 配对末端测序
JP2009529876A (ja) * 2006-03-14 2009-08-27 ゲニゾン バイオサイエンシス インコーポレイテッド 核酸を配列決定するための方法および手段

Also Published As

Publication number Publication date
WO2012079486A1 (zh) 2012-06-21
CN102534811B (zh) 2013-11-20
CN102534811A (zh) 2012-07-04

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