HK1215053A1 - 用作生物合成罗汉果化合物的方法及材料 - Google Patents

用作生物合成罗汉果化合物的方法及材料 Download PDF

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Publication number
HK1215053A1
HK1215053A1 HK16102919.6A HK16102919A HK1215053A1 HK 1215053 A1 HK1215053 A1 HK 1215053A1 HK 16102919 A HK16102919 A HK 16102919A HK 1215053 A1 HK1215053 A1 HK 1215053A1
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Hong Kong
Prior art keywords
methods
biosynthesis
materials
useful
mogroside compounds
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HK16102919.6A
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English (en)
Chinese (zh)
Inventor
Yaoquan Liu
Jo̸rgen HANSEN
Jens Houghton-Larsen
Muthuswamy Panchapagesa MURALI
Sathish Kumar
Nina Nicoline RASMUSSEN
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Evolva Sa
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Publication of HK1215053A1 publication Critical patent/HK1215053A1/zh

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    • C12P33/00—Preparation of steroids
    • C12P33/20—Preparation of steroids containing heterocyclic rings
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C12P33/08—Hydroxylating at 11 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P33/00—Preparation of steroids
    • C12P33/12—Acting on D ring
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    • C12Y303/00—Hydrolases acting on ether bonds (3.3)
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    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C12Y504/99033—Cucurbitadienol synthase (5.4.99.33)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13132—Squalene monooxygenase (1.14.13.132)

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  • Chemical & Material Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
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  • Zoology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
HK16102919.6A 2012-12-04 2013-12-04 用作生物合成罗汉果化合物的方法及材料 HK1215053A1 (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261733220P 2012-12-04 2012-12-04
US201261733220P 2012-12-04
PCT/EP2013/075510 WO2014086842A1 (en) 2012-12-04 2013-12-04 Methods and materials for biosynthesis of mogroside compounds

Publications (1)

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HK1215053A1 true HK1215053A1 (zh) 2016-08-12

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US (4) US9932619B2 (enExample)
EP (1) EP2929043A1 (enExample)
JP (1) JP2015536157A (enExample)
AU (3) AU2015200486A1 (enExample)
BR (1) BR112015013129A2 (enExample)
CA (1) CA2893462A1 (enExample)
HK (1) HK1215053A1 (enExample)
MX (1) MX367859B (enExample)
SG (2) SG10201704575XA (enExample)
WO (1) WO2014086842A1 (enExample)

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CN106350564A (zh) * 2015-07-20 2017-01-25 中国科学院天津工业生物技术研究所 罗汉果醇合成途径中萜类化合物及其糖基化产物的合成方法
JP6949838B2 (ja) 2015-10-29 2021-10-13 フィルメニッヒ インコーポレイテッドＦｉｒｍｅｎｉｃｈ Ｉｎｃｏｒｐｏｒａｔｅｄ 高甘味度甘味料
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CN105713061A (zh) * 2016-03-03 2016-06-29 湖南绿蔓生物科技股份有限公司 一种罗汉果苷v含量≥95％的罗汉果提取物的制备方法
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CN109679987A (zh) * 2018-09-27 2019-04-26 怀化兴科创生物技术有限公司 一个提高甜甙含量相关基因的应用
US12338476B2 (en) 2018-11-07 2025-06-24 Firmenich Incorporated Methods for making high intensity sweeteners
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CN110066785B (zh) * 2019-01-30 2021-01-26 中国医学科学院药用植物研究所 一种罗汉果葫芦二烯醇合酶突变体及其构建方法和应用
CN113646321B (zh) * 2019-02-26 2026-04-21 可口可乐公司 罗汉果苷及其用途
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CN110283224B (zh) * 2019-07-16 2020-08-28 湖南华诚生物资源股份有限公司 一种罗汉果甜苷v的半合成方法
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CN112903835B (zh) * 2021-01-12 2022-08-12 广州泽力医药科技有限公司 罗汉果橘红固体饮料的指纹图谱构建方法及检测方法
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CN113755355A (zh) * 2021-09-30 2021-12-07 河北维达康生物科技有限公司 一种以葡萄糖为底物生物合成罗汉果醇的工程菌株、构建及其应用
JP7873511B2 (ja) * 2022-03-31 2026-06-12 国立台湾大学 トリテルペノイド化合物の調製方法
CN114854722B (zh) * 2022-05-11 2024-01-09 成都普睿法药物研发有限公司 一种季也蒙念珠菌外切β-1,3-葡聚糖酶及其突变体及应用
CN115896136B (zh) * 2022-12-22 2025-12-16 云南农业大学 与葫芦二烯醇羟化相关的细胞色素p450基因及其编码产物
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AU2015200486A1 (en) 2015-03-05
US20150064743A1 (en) 2015-03-05
US9932619B2 (en) 2018-04-03
AU2019250137A1 (en) 2019-10-31
AU2022204430A1 (en) 2022-07-14
US10465222B2 (en) 2019-11-05
US20150322473A1 (en) 2015-11-12
AU2019250137B2 (en) 2022-03-24
JP2015536157A (ja) 2015-12-21
WO2014086842A1 (en) 2014-06-12
US20180346953A1 (en) 2018-12-06
MX367859B (es) 2019-09-09
CA2893462A1 (en) 2014-06-12
MX2015007039A (es) 2016-02-25
US10662458B2 (en) 2020-05-26
SG11201503757RA (en) 2015-06-29
BR112015013129A2 (pt) 2017-07-11
US20180251806A1 (en) 2018-09-06
EP2929043A1 (en) 2015-10-14
US10011859B2 (en) 2018-07-03
WO2014086842A9 (en) 2015-06-18
SG10201704575XA (en) 2017-07-28

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