AU2015200486A1 - Methods and materials for biosynthesis of mogroside compounds - Google Patents

Methods and materials for biosynthesis of mogroside compounds Download PDF

Info

Publication number
AU2015200486A1
AU2015200486A1 AU2015200486A AU2015200486A AU2015200486A1 AU 2015200486 A1 AU2015200486 A1 AU 2015200486A1 AU 2015200486 A AU2015200486 A AU 2015200486A AU 2015200486 A AU2015200486 A AU 2015200486A AU 2015200486 A1 AU2015200486 A1 AU 2015200486A1
Authority
AU
Australia
Prior art keywords
seq
polypeptide
acid sequence
set forth
sequence set
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2015200486A
Other languages
English (en)
Inventor
Jorgen Hansen
Jens Houghton-Larsen
Sathish Kumar
Yaoquan Liu
P.M. Murali
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danstar Ferment AG
Original Assignee
Evolva AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva AG filed Critical Evolva AG
Publication of AU2015200486A1 publication Critical patent/AU2015200486A1/en
Priority to AU2019250137A priority Critical patent/AU2019250137B2/en
Priority to AU2022204430A priority patent/AU2022204430A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/20—Preparation of steroids containing heterocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C12P33/08—Hydroxylating at 11 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/12—Acting on D ring
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y303/00—Hydrolases acting on ether bonds (3.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C12Y504/99033—Cucurbitadienol synthase (5.4.99.33)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13132—Squalene monooxygenase (1.14.13.132)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
AU2015200486A 2012-12-04 2015-02-02 Methods and materials for biosynthesis of mogroside compounds Abandoned AU2015200486A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2019250137A AU2019250137B2 (en) 2012-12-04 2019-10-16 Methods and materials for biosynthesis of mogroside compounds
AU2022204430A AU2022204430A1 (en) 2012-12-04 2022-06-23 Methods and materials for biosynthesis of mogroside compounds

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261733220P 2012-12-04 2012-12-04
US61/733,220 2012-12-04

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2019250137A Division AU2019250137B2 (en) 2012-12-04 2019-10-16 Methods and materials for biosynthesis of mogroside compounds

Publications (1)

Publication Number Publication Date
AU2015200486A1 true AU2015200486A1 (en) 2015-03-05

Family

ID=49709683

Family Applications (3)

Application Number Title Priority Date Filing Date
AU2015200486A Abandoned AU2015200486A1 (en) 2012-12-04 2015-02-02 Methods and materials for biosynthesis of mogroside compounds
AU2019250137A Active AU2019250137B2 (en) 2012-12-04 2019-10-16 Methods and materials for biosynthesis of mogroside compounds
AU2022204430A Abandoned AU2022204430A1 (en) 2012-12-04 2022-06-23 Methods and materials for biosynthesis of mogroside compounds

Family Applications After (2)

Application Number Title Priority Date Filing Date
AU2019250137A Active AU2019250137B2 (en) 2012-12-04 2019-10-16 Methods and materials for biosynthesis of mogroside compounds
AU2022204430A Abandoned AU2022204430A1 (en) 2012-12-04 2022-06-23 Methods and materials for biosynthesis of mogroside compounds

Country Status (10)

Country Link
US (4) US9932619B2 (enExample)
EP (1) EP2929043A1 (enExample)
JP (1) JP2015536157A (enExample)
AU (3) AU2015200486A1 (enExample)
BR (1) BR112015013129A2 (enExample)
CA (1) CA2893462A1 (enExample)
HK (1) HK1215053A1 (enExample)
MX (1) MX367859B (enExample)
SG (2) SG10201704575XA (enExample)
WO (1) WO2014086842A1 (enExample)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2783009B1 (en) 2011-11-23 2016-04-13 Evolva SA Methods and materials for enzymatic synthesis of mogroside compounds
EP2929043A1 (en) 2012-12-04 2015-10-14 Evolva SA Methods and materials for biosynthesis of mogroside compounds
IL287789B2 (en) 2014-09-11 2024-05-01 The State Of Israel Ministry Of Agriculture & Rural Development Agricultural Res Organization Aro Vo Mogroside compounds, methods for their preparation, preparations including them and their uses
CN107466320B (zh) 2014-10-01 2021-11-05 埃沃尔瓦公司 用于生物合成罗汉果苷化合物的方法和材料
EP3208342A4 (en) 2014-10-17 2018-07-04 Suntory Holdings Limited Mogrol glycosyltransferase and gene encoding same
CN104988167A (zh) * 2014-11-21 2015-10-21 中国科学院天津工业生物技术研究所 罗汉果葫芦二烯醇合成酶基因SgCbQ及其应用
CN105801658B (zh) * 2014-12-30 2019-03-19 北京农学院 甜甙及其类似物的制备及作为stat3、erk信号通路靶位点药物在抗肿瘤中的应用
CN106350564A (zh) * 2015-07-20 2017-01-25 中国科学院天津工业生物技术研究所 罗汉果醇合成途径中萜类化合物及其糖基化产物的合成方法
JP6949838B2 (ja) 2015-10-29 2021-10-13 フィルメニッヒ インコーポレイテッドＦｉｒｍｅｎｉｃｈ Ｉｎｃｏｒｐｏｒａｔｅｄ 高甘味度甘味料
CN105713060A (zh) * 2016-01-29 2016-06-29 滁州润海甜叶菊高科有限公司 一种罗汉果中提取糖分工艺
CN105713061A (zh) * 2016-03-03 2016-06-29 湖南绿蔓生物科技股份有限公司 一种罗汉果苷v含量≥95％的罗汉果提取物的制备方法
CN105777837A (zh) * 2016-05-06 2016-07-20 深圳以诺生物制药有限公司 一种新型罗汉果醇衍生物单体
EP3458598A1 (en) * 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
JP6605179B2 (ja) * 2017-03-01 2019-11-13 桂林莱菌生物科技股▲ふん▼有限公司 ラカンカ懸濁細胞中のモグロシドｖ含有量を高める方法
CN111108213B (zh) * 2017-05-03 2024-12-13 弗门尼舍公司 制备高强度甜味剂的方法
CA3064334A1 (en) 2017-06-02 2018-12-06 Givaudan Sa Compositions
EP3638804A1 (en) 2017-06-15 2020-04-22 Evolva SA Production of mogroside compounds in recombinant hosts
CN109385377A (zh) * 2017-08-04 2019-02-26 中国科学院天津工业生物技术研究所 用于生产葫芦二烯醇的工程菌及其制备方法与应用
US12351849B2 (en) * 2018-02-27 2025-07-08 Manus Bio Inc. Microbial production of triterpenoids including mogrosides
JP6814182B2 (ja) * 2018-09-10 2021-01-13 アナリティコン ディスカバリー ゲーエムベーハー 甘味料または甘味増強剤として使用される新規なトリペルテングリコシド
CN109679987A (zh) * 2018-09-27 2019-04-26 怀化兴科创生物技术有限公司 一个提高甜甙含量相关基因的应用
US12338476B2 (en) 2018-11-07 2025-06-24 Firmenich Incorporated Methods for making high intensity sweeteners
CN113286523A (zh) 2018-11-07 2021-08-20 弗门尼舍公司 制备高强度甜味剂的方法
CA3118924A1 (en) 2018-11-09 2020-05-14 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN110066785B (zh) * 2019-01-30 2021-01-26 中国医学科学院药用植物研究所 一种罗汉果葫芦二烯醇合酶突变体及其构建方法和应用
CN113646321B (zh) * 2019-02-26 2026-04-21 可口可乐公司 罗汉果苷及其用途
BR112021026302A2 (pt) * 2019-06-25 2022-03-03 Univ California Método para fazer um triterpeno oxidado a partir de beta-amirina, e, célula microbiana engenheirada para fazer um triterpeno oxidado
CN112194568B (zh) * 2019-07-08 2023-04-14 福建中医药大学 一种长链三萜类化合物及其制备方法和用途
CN110283224B (zh) * 2019-07-16 2020-08-28 湖南华诚生物资源股份有限公司 一种罗汉果甜苷v的半合成方法
US12480146B2 (en) 2019-12-16 2025-11-25 Manus Bio Inc. Microbial production of mogrol and mogrosides
CN120230785A (zh) * 2020-03-17 2025-07-01 可口可乐公司 新的罗汉果苷生产系统和方法
CA3179775A1 (en) 2020-04-20 2021-10-28 Givaudan Sa Compositions
GB202007984D0 (en) 2020-05-28 2020-07-15 Givaudan Sa Compositions
CN112903835B (zh) * 2021-01-12 2022-08-12 广州泽力医药科技有限公司 罗汉果橘红固体饮料的指纹图谱构建方法及检测方法
US20240218403A1 (en) * 2021-04-02 2024-07-04 Ginkgo Bioworks, Inc. Biosynthesis of isoprenoids and precursors thereof
EP4314272A1 (en) * 2021-04-02 2024-02-07 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
EP4314316A2 (en) 2021-06-29 2024-02-07 Firmenich Incorporated Methods for making high intensity sweeteners
WO2023278226A1 (en) * 2021-06-29 2023-01-05 Firmenich Incorporated Mogroside compounds and their comestible use
CN113755355A (zh) * 2021-09-30 2021-12-07 河北维达康生物科技有限公司 一种以葡萄糖为底物生物合成罗汉果醇的工程菌株、构建及其应用
JP7873511B2 (ja) * 2022-03-31 2026-06-12 国立台湾大学 トリテルペノイド化合物の調製方法
CN114854722B (zh) * 2022-05-11 2024-01-09 成都普睿法药物研发有限公司 一种季也蒙念珠菌外切β-1,3-葡聚糖酶及其突变体及应用
CN115896136B (zh) * 2022-12-22 2025-12-16 云南农业大学 与葫芦二烯醇羟化相关的细胞色素p450基因及其编码产物
EP4698673A1 (en) 2023-04-20 2026-02-25 DSM IP Assets B.V. Strains and methods for the production of mogrosides
WO2025144785A2 (en) 2023-12-28 2025-07-03 Dsm Ip Assets B.V. Cells and methods for the production of glycosylated compounds

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6399321B1 (en) 1999-08-18 2002-06-04 National Research Council Of Canada Methods for screening UDP-glucose:glycoprotein glucosyltransferase (UGGT) activity and nucleic acid encoding for UGGT
EP1510573A1 (en) 2003-09-01 2005-03-02 Austria Wirtschaftsservice Gesellschaft mit beschränkter Haftung Method for detoxification of mycotoxins
US7569389B2 (en) * 2004-09-30 2009-08-04 Ceres, Inc. Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics
EP2096177A3 (en) * 2004-12-17 2010-01-13 Metanomics GmbH Process for the production of lutein
WO2006092449A2 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
US8257948B1 (en) 2011-02-17 2012-09-04 Purecircle Usa Method of preparing alpha-glucosyl Stevia composition
US8956677B2 (en) 2005-11-23 2015-02-17 The Coca-Cola Company High-potency sweetener composition with glucosamine and compositions sweetened therewith
RU2425590C2 (ru) 2005-11-23 2011-08-10 Дзе Кока-Кола Компани Интенсивный подсластитель для гидратации и подслащенная гидратирующая композиция
US7927851B2 (en) * 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
WO2008062165A2 (en) 2006-11-22 2008-05-29 The University Of York Monoterpenoid modifying enzymes
US20100132073A1 (en) 2006-12-01 2010-05-27 The University Of York Sesquiterpenoid modifying enzymes
EP2594645A3 (en) * 2007-09-21 2013-11-06 BASF Plant Science GmbH Plants with increased yield
GB0904639D0 (en) 2009-03-18 2009-04-29 Univ York Assay
CN105671108A (zh) 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产
EP2783009B1 (en) 2011-11-23 2016-04-13 Evolva SA Methods and materials for enzymatic synthesis of mogroside compounds
EP2929043A1 (en) 2012-12-04 2015-10-14 Evolva SA Methods and materials for biosynthesis of mogroside compounds

Also Published As

Publication number Publication date
HK1215053A1 (zh) 2016-08-12
US20150064743A1 (en) 2015-03-05
US9932619B2 (en) 2018-04-03
AU2019250137A1 (en) 2019-10-31
AU2022204430A1 (en) 2022-07-14
US10465222B2 (en) 2019-11-05
US20150322473A1 (en) 2015-11-12
AU2019250137B2 (en) 2022-03-24
JP2015536157A (ja) 2015-12-21
WO2014086842A1 (en) 2014-06-12
US20180346953A1 (en) 2018-12-06
MX367859B (es) 2019-09-09
CA2893462A1 (en) 2014-06-12
MX2015007039A (es) 2016-02-25
US10662458B2 (en) 2020-05-26
SG11201503757RA (en) 2015-06-29
BR112015013129A2 (pt) 2017-07-11
US20180251806A1 (en) 2018-09-06
EP2929043A1 (en) 2015-10-14
US10011859B2 (en) 2018-07-03
WO2014086842A9 (en) 2015-06-18
SG10201704575XA (en) 2017-07-28

Similar Documents

Publication Publication Date Title
AU2019250137B2 (en) Methods and materials for biosynthesis of mogroside compounds
AU2022204012C1 (en) Methods and materials for biosynthesis of mogroside compounds
EP3190905B1 (en) Production of steviol glycosides in recombinant hosts
EP4148137A1 (en) Production of steviol glycosides in recombinant hosts
US11248248B2 (en) Production of mogroside compounds in recombinant hosts
US20180327723A1 (en) Production of Glycosylated Nootkatol in Recombinant Hosts

Legal Events

Date Code Title Description
NA Applications received for extensions of time, section 223

Free format text: AN APPLICATION TO EXTEND THE TIME FROM 04 DEC 2013 TO 04 FEB 2015 IN WHICH TO CLAIM THE CONVENTION PRIORITY DATE HAS BEEN FILED .

NB Applications allowed - extensions of time section 223(2)

Free format text: THE TIME IN WHICH TO CLAIM THE CONVENTION PRIORITY DATE HAS BEEN EXTENDED TO 04 FEB 2015 .

MK5 Application lapsed section 142(2)(e) - patent request and compl. specification not accepted