HK1206547A2 - A culture medium for hematopoietic stem cells and the applications thereof as well as the stem cells culture method - Google Patents

A culture medium for hematopoietic stem cells and the applications thereof as well as the stem cells culture method

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Publication number
HK1206547A2
HK1206547A2 HK15107918.7A HK15107918A HK1206547A2 HK 1206547 A2 HK1206547 A2 HK 1206547A2 HK 15107918 A HK15107918 A HK 15107918A HK 1206547 A2 HK1206547 A2 HK 1206547A2
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HK
Hong Kong
Prior art keywords
stem cells
applications
well
culture medium
culture
Prior art date
Application number
HK15107918.7A
Other languages
English (en)
Chinese (zh)
Inventor
劉小青
Original Assignee
深圳富利鑫健康產業發展有限公司 單元 中國生命科學技術集團有限公司 號
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Application filed by 深圳富利鑫健康產業發展有限公司 單元 中國生命科學技術集團有限公司 號 filed Critical 深圳富利鑫健康產業發展有限公司 單元 中國生命科學技術集團有限公司 號
Publication of HK1206547A2 publication Critical patent/HK1206547A2/xx

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
    • C—CHEMISTRY; METALLURGY
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/76—Undefined extracts from plants
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/11—Epidermal growth factor [EGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/113—Acidic fibroblast growth factor (aFGF, FGF-1)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/135—Platelet-derived growth factor [PDGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/14—Erythropoietin [EPO]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/145—Thrombopoietin [TPO]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/165—Vascular endothelial growth factor [VEGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/19—Growth and differentiation factors [GDF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2302—Interleukin-2 (IL-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/235—Leukemia inhibitory factor [LIF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/24—Interferons [IFN]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/25—Tumour necrosing factors [TNF]

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HK15107918.7A 2015-05-28 2015-08-17 A culture medium for hematopoietic stem cells and the applications thereof as well as the stem cells culture method HK1206547A2 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510283776.3A CN104830772A (zh) 2015-05-28 2015-05-28 一种造血干细胞培养基及其应用和干细胞培养方法

Publications (1)

Publication Number Publication Date
HK1206547A2 true HK1206547A2 (en) 2016-01-08

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HK15107918.7A HK1206547A2 (en) 2015-05-28 2015-08-17 A culture medium for hematopoietic stem cells and the applications thereof as well as the stem cells culture method
HK15111033.9A HK1210222A1 (en) 2015-05-28 2015-11-09 A culture medium for hematopoietic stem cells and the applications thereof as well as the stem cells culture method

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Country Status (4)

Country Link
US (1) US20160348065A1 (fr)
EP (1) EP3098307A1 (fr)
CN (1) CN104830772A (fr)
HK (2) HK1206547A2 (fr)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105176925B (zh) * 2015-09-26 2018-08-28 友康恒业生物科技（北京）有限公司 一种免疫细胞无血清培养基及其用途
CN105567628B (zh) * 2016-01-30 2019-07-12 令世鑫 一种全悬浮培养mdck细胞的低血清培养基
CN105543174A (zh) * 2016-03-01 2016-05-04 赛百慷（上海）生物技术股份有限公司 一种肿瘤细胞生长促进添加剂及其使用方法
CN105713870A (zh) * 2016-03-04 2016-06-29 佰通生物技术(苏州)有限公司 一种基于集落刺激因子1对干细胞的活化方法
CN106148283A (zh) * 2016-08-08 2016-11-23 安徽惠恩生物科技股份有限公司 一种造血干细胞贴壁性好的培养基
CN107034189A (zh) * 2017-05-31 2017-08-11 东莞市保莱生物科技有限公司 一种造血干细胞培养方法
CN111909888A (zh) * 2017-06-18 2020-11-10 广东博溪生物科技有限公司 用于构建屏障加强体外重组表皮模型的ta培养液
CN109576222A (zh) * 2017-09-29 2019-04-05 重庆金时代生物技术有限公司 一种造血干细胞培养基
CN109576223A (zh) * 2017-09-29 2019-04-05 重庆金时代生物技术有限公司 一种胎盘血造血干细胞的培养方法
CN107904205A (zh) * 2017-12-01 2018-04-13 重庆金时代生物技术有限公司 一种用于培养造血干细胞的培养基及其制备方法
CN108315300A (zh) * 2018-02-11 2018-07-24 大连金玛健康产业发展有限公司 一种细胞因子组合物在体外培养造血干细胞中的应用
CN108277203B (zh) * 2018-04-08 2020-07-24 浙江大学 一种体外维持人造血干祖细胞干性的培养基及方法
CN108559728A (zh) * 2018-05-08 2018-09-21 李顺河 一种干细胞培养基及其应用和干细胞培养方法
CN108795857A (zh) * 2018-06-20 2018-11-13 四川瑞兴和弘健康咨询有限公司 一种细胞培养液、利用该细胞培养液制备富血小板血浆的方法及制得的富血小板血浆
CN109370988A (zh) * 2018-11-03 2019-02-22 章毅 干细胞体外扩增培养体系及其方法
CN109735491A (zh) * 2019-01-16 2019-05-10 广东美赛尔细胞生物科技有限公司 一种可扩增造血干细胞的无血清培养基及其制备方法
CN112210525A (zh) * 2019-07-11 2021-01-12 江南大学 一种无血清培养体系及其在细胞培养肉中的应用
CN110564680B (zh) * 2019-08-27 2021-05-04 西安艾尔菲生物科技有限公司 人脐带间充质干细胞无血清培养基和制备方法及人脐带间充质干细胞无血清培养液获取方法
KR20220093196A (ko) * 2019-11-04 2022-07-05 루비우스 테라퓨틱스, 아이엔씨. 타우린 또는 하이포타우린을 사용하여 제핵 적혈구 세포를 생성하는 방법
JP2023500327A (ja) * 2019-11-04 2023-01-05 ルビウス セラピューティクス， インコーポレイテッド ミオイノシトールを使用して除核赤芽球細胞を生成する方法
CN110882276B (zh) * 2019-11-23 2021-07-06 博雅干细胞科技有限公司 细胞治疗组合物及治疗血管病变的方法
CN110840914B (zh) * 2019-11-23 2021-07-06 博雅干细胞科技有限公司 细胞治疗剂用于缓解或改善血管病变的方法
CN110713974A (zh) * 2019-11-25 2020-01-21 深圳科学之门生物工程有限公司 一种干细胞培养基及其制备方法
CN111748522A (zh) * 2020-07-31 2020-10-09 贵州医科大学附属医院 一种干细胞培养基及其应用
AU2021348257A1 (en) * 2020-09-28 2023-03-02 Ludwig Institute For Cancer Research Compositions and methods for increasing stem cell function
AU2021350472A1 (en) * 2020-09-28 2023-03-02 Ludwig Institute For Cancer Research Compositions and methods for increasing stem cell function
CN112553158A (zh) * 2020-12-14 2021-03-26 北京博奥体质宝健康科技有限公司 一种多能修复细胞mdmc的提取制备工艺
CN113151172A (zh) * 2021-05-14 2021-07-23 郑州优倍得生物科技有限公司 一种用于扩增脐带血造血干细胞的培养基
CN114480271A (zh) * 2022-02-18 2022-05-13 温益雄 促进顽固性压疮愈合的脐带间充质干细胞外泌体及其制备方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5405772A (en) * 1993-06-18 1995-04-11 Amgen Inc. Medium for long-term proliferation and development of cells
WO1998006422A1 (fr) * 1996-08-13 1998-02-19 Fujisawa Pharmaceutical Co., Ltd. Agents de proliferation des cellules souches hematopoietiques
US5945337A (en) * 1996-10-18 1999-08-31 Quality Biological, Inc. Method for culturing CD34+ cells in a serum-free medium
WO2000070022A2 (fr) * 1999-05-14 2000-11-23 City Of Hope Expansion ex vivo de cellules souches hematopoietiques multipotentes mammaliennes
EP1312668A4 (fr) * 2000-08-25 2004-07-07 Asahi Chemical Ind Milieu de culture de cellule souche et procede de culture utilisant ce milieu
US20060205071A1 (en) * 2003-07-17 2006-09-14 Gamida-Cell Ltd. Methods for ex-vivo expanding stem/progenitor cells
JP5420837B2 (ja) * 2004-09-08 2014-02-19 ウィスコンシン アラムニ リサーチ ファンデーション 胚幹細胞の培地及び培養
JP5409222B2 (ja) * 2009-09-10 2014-02-05 学校法人慶應義塾 造血幹細胞の培養方法
TWI563922B (zh) * 2012-03-30 2017-01-01 Ming-Jia Weng Food recipes for nutrition and maintenance of stem cells
CN104013644B (zh) * 2014-05-30 2017-05-10 成都清科生物科技有限公司 一种用于皮肤衰老修复的间充质干细胞的条件培养基制剂

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