CN105543174A - 一种肿瘤细胞生长促进添加剂及其使用方法 - Google Patents

一种肿瘤细胞生长促进添加剂及其使用方法 Download PDF

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CN105543174A
CN105543174A CN201610113584.2A CN201610113584A CN105543174A CN 105543174 A CN105543174 A CN 105543174A CN 201610113584 A CN201610113584 A CN 201610113584A CN 105543174 A CN105543174 A CN 105543174A
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tumor cell
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王悦
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Subway (shanghai) Biotechnology Ltd By Share Ltd
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Abstract

本发明公开了一种肿瘤细胞生长促进添加剂；所述添加剂其组成成分及浓度为：丙酮酸钠11mg/ml，β巯基乙醇5mM，谷氨酰胺200mM，碳酸氢钠300mM，4-羟乙基哌嗪乙磺酸500mM，胰岛素10ug/ml，黄体酮2uM，丁二胺6mM，硒酸钠3uM，人全铁转铁蛋白10mg/ml，重组人表皮细胞生长因子10ng/ml。上述肿瘤细胞生长促进添加剂其使用方法为：将上述添加剂稀释100倍后，作为最终使用浓度，直接添加到培养基上使用。本发明的肿瘤细胞生长促进添加剂及其使用方法，其能够应用在肿瘤细胞方面，促进肿瘤细胞生长，使用范围广泛。

Description

一种肿瘤细胞生长促进添加剂及其使用方法
技术领域
本发明涉及一种肿瘤细胞培养添加剂，特别涉及一种肿瘤细胞生长促进添加剂及其使用方法，属于肿瘤细胞培养技术领域。
背景技术
目前恶性肿瘤治疗方案首选手术切除原发病灶，但是有些恶性肿瘤患者在就诊时已处于肿瘤晚期，无法行手术治疗，且手术切除原发灶后，肿瘤复发均影响了肿瘤治疗效果，降低了恶性肿瘤患者生存期，化疗治疗是提高肿瘤治疗效果，改善患者预后并延长患者生存期的重要手段之一，目前恶性肿瘤的化疗治疗主要是采取全身应用化疗药物，在应用化疗药物前，通过体外培养肿瘤组织原代细胞，从而筛选出敏感性化疗药物，指导临床制定化疗方案；另外肿瘤细胞培养也是研究癌变机理、抗癌药的敏感性、肿瘤细胞和癌分子生物学特性的重要手段；现有的培养过程比较缓慢，对于一些恶化速度比较快的肿瘤细胞其很难完成快速培养。
发明内容
（一）要解决的技术问题
为解决上述问题，本发明提出了一种肿瘤细胞生长促进添加剂及其使用方法，其能够应用在肿瘤细胞方面，促进肿瘤细胞生长，使用范围广泛。
（二）技术方案
本发明的肿瘤细胞生长促进添加剂，所述添加剂其组成成分及浓度为：丙酮酸钠11mg/ml，β巯基乙醇5mM，谷氨酰胺200mM，碳酸氢钠300mM，4-羟乙基哌嗪乙磺酸500mM，胰岛素10ug/ml，黄体酮2uM，丁二胺6mM，硒酸钠3uM，人全铁转铁蛋白10mg/ml，重组人表皮细胞生长因子10ng/ml。
上述肿瘤细胞生长促进添加剂其使用方法为：将上述添加剂稀释100倍后，作为最终使用浓度，直接添加到培养基上使用。
有益效果
与现有技术相比，本发明的肿瘤细胞生长促进添加剂及其使用方法，其能够应用在肿瘤细胞方面，促进肿瘤细胞生长，使用范围广泛。
具体实施方式
本发明肿瘤细胞生长促进添加剂，所述添加剂其组成成分及浓度为：丙酮酸钠11mg/ml，β巯基乙醇5mM，谷氨酰胺200mM，碳酸氢钠300mM，4-羟乙基哌嗪乙磺酸500mM，胰岛素10ug/ml，黄体酮2uM，丁二胺6mM，硒酸钠3uM，人全铁转铁蛋白10mg/ml，重组人表皮细胞生长因子10ng/ml。
上述肿瘤细胞生长促进添加剂其使用方法为：将上述添加剂稀释100倍后，作为最终使用浓度，直接添加到培养基上使用。
上面所述的实施例仅仅是对本发明的优选实施方式进行描述，并非对本发明的构思和范围进行限定。在不脱离本发明设计构思的前提下，本领域普通人员对本发明的技术方案做出的各种变型和改进，均应落入到本发明的保护范围，本发明请求保护的技术内容，已经全部记载在权利要求书中。

Claims (2)

1.一种肿瘤细胞生长促进添加剂，其特征在于：所述添加剂其组成成分及浓度为：丙酮酸钠11mg/ml，β巯基乙醇5mM，谷氨酰胺200mM，碳酸氢钠300mM，4-羟乙基哌嗪乙磺酸500mM，胰岛素10ug/ml，黄体酮2uM，丁二胺6mM，硒酸钠3uM，人全铁转铁蛋白10mg/ml，重组人表皮细胞生长因子10ng/ml。
2.一种肿瘤细胞生长促进添加剂其使用方法，其特征在于，所述方法为：将上述添加剂稀释100倍后，作为最终使用浓度，直接添加到培养基上使用。
CN201610113584.2A 2016-03-01 2016-03-01 一种肿瘤细胞生长促进添加剂及其使用方法 Pending CN105543174A (zh)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102399742A (zh) * 2011-09-30 2012-04-04 东莞中山大学研究院 用于哺乳动物唾液腺上皮细胞和唾液腺干细胞培养的无血清培养液
CN103060264A (zh) * 2012-12-20 2013-04-24 上海市第十人民医院 一种干细胞培养基及其应用和干细胞培养方法
CN103966167A (zh) * 2013-02-05 2014-08-06 高全立 用于原代培养肿瘤细胞的细胞培养组合物及用途
CN104830772A (zh) * 2015-05-28 2015-08-12 深圳富利鑫健康产业发展有限公司 一种造血干细胞培养基及其应用和干细胞培养方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102399742A (zh) * 2011-09-30 2012-04-04 东莞中山大学研究院 用于哺乳动物唾液腺上皮细胞和唾液腺干细胞培养的无血清培养液
CN103060264A (zh) * 2012-12-20 2013-04-24 上海市第十人民医院 一种干细胞培养基及其应用和干细胞培养方法
CN103966167A (zh) * 2013-02-05 2014-08-06 高全立 用于原代培养肿瘤细胞的细胞培养组合物及用途
CN104830772A (zh) * 2015-05-28 2015-08-12 深圳富利鑫健康产业发展有限公司 一种造血干细胞培养基及其应用和干细胞培养方法

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