HK1100960A1 - 用於產生和擴增用於靈敏檢測和分析 甲基化的 文庫的方法和組合物 - Google Patents

用於產生和擴增用於靈敏檢測和分析 甲基化的 文庫的方法和組合物

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Publication number
HK1100960A1
HK1100960A1 HK07105296.3A HK07105296A HK1100960A1 HK 1100960 A1 HK1100960 A1 HK 1100960A1 HK 07105296 A HK07105296 A HK 07105296A HK 1100960 A1 HK1100960 A1 HK 1100960A1
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Hong Kong
Prior art keywords
dna
compositions
analysis
generating
methods
Prior art date
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HK07105296.3A
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English (en)
Inventor
Vladimir L Makarov
Emmanuel Kamberov
Tong Sun
Jonathon H Pinter
Brendan J Tarrier
Eric E Bruening
Takao Kurihara
Tim Tesmer
Joseph M Mmwirichia
Original Assignee
Rubicon Genomics Inc
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Application filed by Rubicon Genomics Inc filed Critical Rubicon Genomics Inc
Publication of HK1100960A1 publication Critical patent/HK1100960A1/zh

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1072Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK07105296.3A 2004-03-08 2007-05-18 用於產生和擴增用於靈敏檢測和分析 甲基化的 文庫的方法和組合物 HK1100960A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55194104P 2004-03-08 2004-03-08
PCT/US2005/006979 WO2005090607A1 (en) 2004-03-08 2005-03-03 Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation

Publications (1)

Publication Number Publication Date
HK1100960A1 true HK1100960A1 (zh) 2007-10-05

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Family Applications (2)

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HK12102762.8A HK1162611A1 (zh) 2004-03-08 2007-05-18 用於生成及擴增 庫以供 甲基化的靈敏檢測及分析用的方法
HK07105296.3A HK1100960A1 (zh) 2004-03-08 2007-05-18 用於產生和擴增用於靈敏檢測和分析 甲基化的 文庫的方法和組合物

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HK12102762.8A HK1162611A1 (zh) 2004-03-08 2007-05-18 用於生成及擴增 庫以供 甲基化的靈敏檢測及分析用的方法

Country Status (5)

Country Link
US (3) US8440404B2 (zh)
EP (3) EP2380993B1 (zh)
CA (2) CA2902980A1 (zh)
HK (2) HK1162611A1 (zh)
WO (1) WO2005090607A1 (zh)

Families Citing this family (105)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004050915A1 (en) * 2002-12-02 2004-06-17 Solexa Limited Determination of methylation of nucleic acid sequences
JP4773338B2 (ja) * 2003-03-07 2011-09-14 ルビコン ゲノミクス, インコーポレイテッド Dna重合プロセスにより生成される全ゲノムおよび全トランスクリプトームライブラリーの増幅および分析
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
US7799525B2 (en) * 2003-06-17 2010-09-21 Human Genetic Signatures Pty Ltd. Methods for genome amplification
CA2537810C (en) 2003-09-04 2012-12-18 Human Genetic Signatures Pty Ltd Nucleic acid detection assay
US7459274B2 (en) * 2004-03-02 2008-12-02 Orion Genomics Llc Differential enzymatic fragmentation by whole genome amplification
EP2380993B1 (en) 2004-03-08 2015-12-23 Rubicon Genomics, Inc. Method for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
US20100273151A1 (en) * 2004-05-28 2010-10-28 Fred Hutchinson Cancer Research Center Genome-wide analysis of palindrome formation and dna methylation
WO2006026828A1 (en) * 2004-09-10 2006-03-16 Human Genetic Signatures Pty Ltd Amplification blocker comprising intercalating nucleic acids (tna) containing intercalating pseudonucleotides (ipn)
KR20060026595A (ko) * 2004-09-21 2006-03-24 (주)지노믹트리 제한효소와 dna 칩을 이용한 프로모터의 메틸화 검출방법
EP1828411B1 (en) * 2004-12-03 2012-11-07 Human Genetic Signatures PTY Ltd Methods for simplifying microbial nucleic acids by chemical modification of cytosines
US20060160116A1 (en) * 2004-12-16 2006-07-20 The Regents Of The University Of California Repetitive sequence-free DNA libraries
JP2008524990A (ja) * 2004-12-23 2008-07-17 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド ヒトパピローマウイルスの検出
CN101203618B (zh) * 2005-05-26 2013-03-13 人类遗传标记控股有限公司 使用含有非常规碱基的引物的等温链置换扩增
US20070031857A1 (en) 2005-08-02 2007-02-08 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of DNA, including using multiple enzymes in a single reaction
EP1924705A1 (en) 2005-08-02 2008-05-28 Rubicon Genomics, Inc. Isolation of cpg islands by thermal segregation and enzymatic selection-amplification method
US8343738B2 (en) * 2005-09-14 2013-01-01 Human Genetic Signatures Pty. Ltd. Assay for screening for potential cervical cancer
AU2006339538A1 (en) 2005-11-08 2007-09-13 Euclid Diagnostics Llc Materials and methods for assaying for methylation of CpG islands associated with genes in the evaluation of cancer
WO2007081791A2 (en) * 2006-01-04 2007-07-19 The Johns Hopkins University Compare-ms:method rapid, sensitive and accurate detection of dna methylation
US7901882B2 (en) * 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
EP1882746A3 (en) * 2006-07-27 2008-03-19 Epigenomics AG Method for detecting a methylation pattern
JP2008136404A (ja) * 2006-11-30 2008-06-19 Sysmex Corp Dnaメチル化検出における非メチル化シトシン変換処理後のdna量の確認方法
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
AU2008229628A1 (en) * 2007-03-16 2008-09-25 Human Genetic Signatures Pty Ltd Assay for gene expression
US8999634B2 (en) * 2007-04-27 2015-04-07 Quest Diagnostics Investments Incorporated Nucleic acid detection combining amplification with fragmentation
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
CA2694820C (en) * 2007-07-30 2015-06-16 F. Hoffman-La Roche Ag Methods of identification using methylation of cpg
WO2009021141A1 (en) * 2007-08-07 2009-02-12 The Johns Hopkins University Comprehensive high throughput arrays for relative methylation
EP2188389A4 (en) * 2007-08-15 2011-12-07 Univ Hong Kong METHOD AND COMPOSITIONS FOR BISULFIT DNA SEQUENCING WITH HIGH PERFORMANCE AND BENEFITS
US8748100B2 (en) 2007-08-30 2014-06-10 The Chinese University Of Hong Kong Methods and kits for selectively amplifying, detecting or quantifying target DNA with specific end sequences
US20090099040A1 (en) * 2007-10-15 2009-04-16 Sigma Aldrich Company Degenerate oligonucleotides and their uses
WO2009067743A1 (en) 2007-11-27 2009-06-04 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
CA2709632A1 (en) * 2007-12-20 2009-07-02 Human Genetic Signatures Pty Ltd Elimination of contaminants associated with nucleic acid amplification
DK3064599T3 (en) * 2008-02-15 2019-04-08 Synthetic Genomics Inc METHODS IN VITRO FOR COMBINING AND COMBINATORY CONSTRUCTION OF NUCLEIC ACID MOLECULES
CA2719795A1 (en) * 2008-03-27 2009-10-01 Greene, Tweed Of Delaware, Inc. Inert substrate-bonded fluoroelastomer components and related methods
US20090253121A1 (en) * 2008-04-04 2009-10-08 Micah Halpern Method for amt-rflp dna fingerprinting
WO2009132315A1 (en) * 2008-04-24 2009-10-29 Life Technologies Corporation Method of sequencing and mapping target nucleic acids
US20100273164A1 (en) * 2009-03-24 2010-10-28 President And Fellows Of Harvard College Targeted and Whole-Genome Technologies to Profile DNA Cytosine Methylation
WO2010114821A1 (en) * 2009-03-30 2010-10-07 Zymo Research Corporation Genomic dna methylation analysis
WO2011011823A1 (en) * 2009-07-29 2011-02-03 Pyrobett Pte Ltd Method and apparatus for conducting an assay
US8889353B2 (en) 2009-09-23 2014-11-18 Koninklijke Philips N.V. Method for monitoring the bisulfite-mediated conversion of DNA
EP2483426A4 (en) * 2009-10-02 2013-04-10 Ct For Addiction And Mental Health METHOD FOR ANALYZING METHYLATION PROFILES OF ACELLULAR CIRCULATING DNA IN BODY FLUIDS
US20130065776A1 (en) * 2009-12-04 2013-03-14 Qiagen Gmbh Selective enrichment of non-methylated nucleic acids
US20130065233A1 (en) * 2010-03-03 2013-03-14 Zymo Research Corporation Detection of dna methylation
CN102796808B (zh) 2011-05-23 2014-06-18 深圳华大基因科技服务有限公司 甲基化高通量检测方法
WO2012166425A2 (en) 2011-05-27 2012-12-06 President And Fellows Of Harvard College Methods of amplifying whole genome of a single cell
US20130041047A1 (en) * 2011-06-01 2013-02-14 Aros Applied Biotechnology As Urinary Methylation Markers for Bladder Cancer
WO2013033380A1 (en) * 2011-08-31 2013-03-07 Genentech, Inc. Diagnostic markers
MY167564A (en) 2011-09-07 2018-09-14 Human Genetic Signatures Pty Ltd Molecular detection assay
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
GB201119903D0 (en) * 2011-11-17 2011-12-28 Univ Vilnius Nucleic acid production and sequence analysis
US9977861B2 (en) * 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
US10706957B2 (en) 2012-09-20 2020-07-07 The Chinese University Of Hong Kong Non-invasive determination of methylome of tumor from plasma
US9732390B2 (en) 2012-09-20 2017-08-15 The Chinese University Of Hong Kong Non-invasive determination of methylome of fetus or tumor from plasma
NZ717423A (en) * 2012-09-20 2017-08-25 Univ Hong Kong Chinese Non-invasive determination of methylome of fetus or tumor from plasma
CA2892043C (en) * 2012-11-21 2022-01-11 Courtagen Life Sciences Inc. A method of removing amplicons of a non-target nucleic acid having one or more methylated cytosines from a sample
WO2014160233A1 (en) * 2013-03-13 2014-10-02 Abbott Molecular Inc. Systems and methods for isolating nucleic acids
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
WO2014145291A1 (en) 2013-03-15 2014-09-18 Theranos, Inc. Nucleic acid amplification
BR112015023398A8 (pt) 2013-03-15 2022-08-23 Theranos Inc Métodos para amplificar ácidos nucléicos
EP2970960B1 (en) 2013-03-15 2019-03-13 Theranos IP Company, LLC Nucleic acid amplification
CN105247071B (zh) * 2013-04-01 2018-11-23 基因特力株式会社 使用等位基因特异的反应性引物的核酸扩增方法
CN103555856B (zh) * 2013-11-15 2015-02-11 复旦大学 一种全基因组dna甲基化的导向测序技术
JP2016537003A (ja) 2013-11-18 2016-12-01 ルビコン ゲノミクス インコーポレイテッド バックグラウンド減少のための分解可能なアダプター
AU2015280069B2 (en) 2014-06-23 2021-08-12 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
US11473131B2 (en) 2014-08-22 2022-10-18 The Trustees Of The University Of Pennsylvania Methods for detecting genomic DNA methylation
AU2015336938B2 (en) * 2014-10-20 2022-01-27 Commonwealth Scientific And Industrial Research Organisation Genome methylation analysis
US20180143198A1 (en) * 2014-12-26 2018-05-24 Peking University Method for detecting differentially methylated cpg islands associated with abnormal state of human body
EP3262191B2 (en) 2015-02-24 2023-06-14 Zymo Research Corporation Assays to determine dna methylation and dna methylation markers of cancer
AU2016281718B2 (en) * 2015-06-24 2022-03-31 Dana-Farber Cancer Institute, Inc. Selective degradation of wild-type DNA and enrichment of mutant alleles using nuclease
WO2017021449A1 (en) * 2015-08-06 2017-02-09 F. Hoffmann-La Roche Ag Target enrichment by single probe primer extension
EP3334834B1 (en) * 2015-08-12 2020-05-13 Circulogene Theranostics, LLC Method of preparing cell free nucleic acid molecules by in situ amplification
AU2016319110B2 (en) * 2015-09-11 2022-01-27 The General Hospital Corporation Full interrogation of nuclease DSBs and sequencing (FIND-seq)
JP2018529353A (ja) 2015-09-30 2018-10-11 ザ ジェネラル ホスピタル コーポレイション 配列決定法による切断事象の包括的生体外報告(CIRCLE−seq)
US10913986B2 (en) 2016-02-01 2021-02-09 The Board Of Regents Of The University Of Nebraska Method of identifying important methylome features and use thereof
ES2914396T3 (es) * 2016-03-22 2022-06-10 Vib Vzw Medios y métodos de amplificación de secuencias de nucleótidos
CN108699554B (zh) * 2016-05-27 2019-11-01 深圳市海普洛斯生物科技有限公司 一种适用于超微量dna测序的接头及其应用
EP3508588A4 (en) * 2016-09-02 2020-05-27 Fujifilm Wako Pure Chemical Corporation METHOD FOR AMPLIFYING METHYLATED DNA, METHOD FOR DETERMINING DNA METHYLATION, AND METHOD FOR DETERMINING THE APPEARANCE OF CANCER
US10190155B2 (en) * 2016-10-14 2019-01-29 Nugen Technologies, Inc. Molecular tag attachment and transfer
CA3046953A1 (en) 2016-12-12 2018-06-21 Dana Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
CN106701939A (zh) * 2016-12-22 2017-05-24 中国热带农业科学院热带生物技术研究所 一种胞嘧啶甲基化挖掘的方法
US11130991B2 (en) 2017-03-08 2021-09-28 The University Of Chicago Method for highly sensitive DNA methylation analysis
CN110662845A (zh) * 2017-05-01 2020-01-07 小利兰·斯坦福大学托管委员会 使用磁阻生物传感器阵列表征dna甲基化
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
WO2019040650A1 (en) 2017-08-23 2019-02-28 The General Hospital Corporation GENETICALLY MODIFIED CRISPR-CAS9 NUCLEASES HAVING MODIFIED PAM SPECIFICITY
US11667952B2 (en) 2017-08-24 2023-06-06 Takara Bio Usa, Inc. Methods of producing nucleic acids using oligonucleotides modified by a stimulus
WO2019070593A1 (en) * 2017-10-04 2019-04-11 Centrillion Technologies, Inc. METHOD AND SYSTEM FOR ENZYMATIC SYNTHESIS OF OLIGONUCLEOTIDES
US11725228B2 (en) 2017-10-11 2023-08-15 The General Hospital Corporation Methods for detecting site-specific and spurious genomic deamination induced by base editing technologies
WO2019159184A1 (en) * 2018-02-18 2019-08-22 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Cell free dna deconvolution and use thereof
EP3542903A1 (en) * 2018-03-22 2019-09-25 IMEC vzw Memory writing
US20190367909A1 (en) * 2018-04-02 2019-12-05 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
WO2019204378A1 (en) 2018-04-17 2019-10-24 The General Hospital Corporation Sensitive in vitro assays for substrate preferences and sites of nucleic acid binding, modifying, and cleaving agents
EP3953471A4 (en) * 2019-04-09 2023-02-01 Arc Bio, LLC COMPOSITIONS AND METHODS OF DEPLETION BASED ON NUCLEOTIDE MODIFICATION
EP3987056A4 (en) * 2019-06-24 2023-07-26 Chromacode, Inc. METHODS FOR THE DIFFERENTIAL QUANTIFICATION OF AMINO ACIDS
CN110343742B (zh) * 2019-07-23 2023-03-21 中国海洋大学 一种用于高通量测序文库制备的微量贝类dna提取方法
CN112176419B (zh) * 2019-10-16 2022-03-22 中国医学科学院肿瘤医院 一种检测ctDNA中肿瘤特异基因的变异和甲基化的方法
CN110938674B (zh) * 2019-12-05 2024-03-19 广州金域医学检验集团股份有限公司 甲基化测序dna文库的构建方法及其应用
CN113249493A (zh) * 2021-03-05 2021-08-13 浙江省血液中心 一种人血小板同种抗原系统等位基因分型的实时荧光pcr方法、探针、引物及试剂盒
CN113552191B (zh) * 2021-07-28 2023-11-21 江苏师范大学 基于多层dna放大回路检测甲基化dna的比例型电化学传感器的构建方法
CN113981548B (zh) * 2021-11-24 2023-07-11 竹石生物科技(苏州)有限公司 Dna甲基化测序文库的制备方法和甲基化检测方法
CN114381501A (zh) * 2021-12-30 2022-04-22 翌圣生物科技(上海)股份有限公司 一种简便的高通量dna甲基化检测方法
IL293202A (en) * 2022-05-22 2023-12-01 Nucleix Ltd Useful combinations of restriction enzymes
WO2024006712A1 (en) * 2022-06-27 2024-01-04 Arima Genomics, Inc. Methods for preparation and analysis of proximity-ligated nucleic acids from single cells

Family Cites Families (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6107023A (en) * 1988-06-17 2000-08-22 Genelabs Technologies, Inc. DNA amplification and subtraction techniques
JPH04501353A (ja) * 1988-07-26 1992-03-12 ジエネラブス・テクノロジーズ・インコーポレイテツド Rna及びdna増幅法
US5106727A (en) * 1989-04-27 1992-04-21 Life Technologies, Inc. Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
CZ291877B6 (cs) * 1991-09-24 2003-06-18 Keygene N.V. Způsob amplifikace přinejmenším jednoho restrikčního fragmentu z výchozí DNA a způsob přípravy sestavy amplifikovaných restrikčních fragmentů
US5405760A (en) * 1992-04-30 1995-04-11 New England Biolabs, Inc. Process for producing recombinant McrBC endonuclease and cleavage of methylated DNA
DE4218152A1 (de) * 1992-06-02 1993-12-09 Boehringer Mannheim Gmbh Simultane Sequenzierung von Nukleinsäuren
US5514545A (en) * 1992-06-11 1996-05-07 Trustees Of The University Of Pennsylvania Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
WO1994024307A1 (en) * 1993-04-16 1994-10-27 Forbio Pty. Ltd. Method of random amplification of polymorphic dna
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
FR2721945B1 (fr) 1994-07-04 1996-10-18 David Fabrice Accroissement genique, un procede d'amplicication genique isotherme et ses applications
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
US5871920A (en) * 1994-09-22 1999-02-16 Whitehead Institute For Biomedical Research Daz: a gene associated with azoospermia
GB9422891D0 (en) 1994-11-12 1995-01-04 Univ Nottingham Improvements in and relating to the amplification and identification of nucleotide sequences
JPH08173164A (ja) 1994-12-22 1996-07-09 Hitachi Ltd Dna調製法
US5565340A (en) * 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
US5994058A (en) * 1995-03-20 1999-11-30 Genome International Corporation Method for contiguous genome sequencing
US5814444A (en) * 1995-06-07 1998-09-29 University Of Washington Methods for making and using single-chromosome amplfication libraries
AU723900B2 (en) * 1996-02-14 2000-09-07 Akzo Nobel N.V. Isolation and amplification of nucleic acid materials
WO1998002575A1 (en) 1996-07-16 1998-01-22 Periannan Senapathy Method for contiguous genome sequencing
GB9620769D0 (en) 1996-10-04 1996-11-20 Brax Genomics Ltd Nucleic acid sequencing
US5968743A (en) * 1996-10-14 1999-10-19 Hitachi, Ltd. DNA sequencing method and reagents kit
US6013438A (en) * 1996-11-27 2000-01-11 Baylor College Of Medicine Assay for detecting apoptotic cells
US6060245A (en) * 1996-12-13 2000-05-09 Stratagene Methods and adaptors for generating specific nucleic acid populations
US6197557B1 (en) 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
KR20010005544A (ko) * 1997-03-21 2001-01-15 그레그 펄쓰 Vntr 대립 유전자의 추출 및 이용방법
US6124120A (en) * 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US5932451A (en) * 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
DE19754482A1 (de) * 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
DE19813317A1 (de) * 1998-03-26 1999-09-30 Roche Diagnostics Gmbh Verbessertes Verfahren zur Primer Extension Präamplifikations-PCR
ATE244771T1 (de) 1998-07-29 2003-07-15 Keygene Nv Verfahren zur erkennung von nukleinsäuremethylierungen durch aflp
JP2000057713A (ja) * 1998-08-05 2000-02-25 Mitsubishi Electric Corp 光ディスクの欠陥管理方法、光ディスク装置、及び、光ディスク
US6235502B1 (en) * 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
IL141749A0 (en) 1998-09-18 2002-03-10 Micromet Ag Dna amplification of a single cell
US6605432B1 (en) * 1999-02-05 2003-08-12 Curators Of The University Of Missouri High-throughput methods for detecting DNA methylation
US20010046669A1 (en) 1999-02-24 2001-11-29 Mccobmie William R. Genetically filtered shotgun sequencing of complex eukaryotic genomes
EP1190092A2 (en) * 1999-04-06 2002-03-27 Yale University Fixed address analysis of sequence tags
US6521428B1 (en) * 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
WO2001009384A2 (en) 1999-07-29 2001-02-08 Genzyme Corporation Serial analysis of genetic alterations
US6403319B1 (en) * 1999-08-13 2002-06-11 Yale University Analysis of sequence tags with hairpin primers
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
AU2001232485A1 (en) * 2000-01-13 2001-07-24 Amsterdam Support Diagnostics B.V. A universal nucleic acid amplification system for nucleic acids in a sample
US6379932B1 (en) 2000-07-17 2002-04-30 Incyte Genomics, Inc. Single primer PCR amplification of RNA
AU2002213882A1 (en) 2000-09-08 2002-03-22 Biomerieux B.V.A. Attenuated hiv strains and use thereof
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
US6893820B1 (en) 2001-01-31 2005-05-17 The Ohio State University Research Foundation Detection of methylated CpG rich sequences diagnostic for malignant cells
WO2002065093A2 (en) * 2001-02-14 2002-08-22 Baylor College Of Medicine Methods and compositions of amplifying rna
AU2002252279B2 (en) 2001-03-09 2005-05-12 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences
CA2443999A1 (en) * 2001-04-16 2002-10-24 Applera Corporation Methods and compositions for nucleotide analysis
WO2002103054A1 (en) 2001-05-02 2002-12-27 Rubicon Genomics Inc. Genome walking by selective amplification of nick-translate dna library and amplification from complex mixtures of templates
JP2002345466A (ja) * 2001-05-08 2002-12-03 Takara Bio Inc Dnaの増幅方法
US6638722B2 (en) * 2001-06-13 2003-10-28 Invitrogen Corporation Method for rapid amplification of DNA
EP1275738A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Method for random cDNA synthesis and amplification
US6632611B2 (en) * 2001-07-20 2003-10-14 Affymetrix, Inc. Method of target enrichment and amplification
US20040175715A1 (en) 2001-08-21 2004-09-09 Burgoyne Leigh A. Method and device for simultaneously molecularly cloning and polylocus profiling of genomes or genomes mixtures
WO2003025215A1 (en) 2001-09-14 2003-03-27 The University Of Queensland Detection of dna methylation
AU2002341891A1 (en) 2001-09-26 2003-04-07 Epigenx Pharmaceuticals, Inc. Assays for dna methylation changes
US20030099997A1 (en) * 2001-10-24 2003-05-29 Bestor Timothy H. Method for gene identification based on differential DNA methylation
US20030232371A1 (en) 2001-10-24 2003-12-18 Bestor Timothy H. Methods for detecting methylated promoters based on differential DNA methylation
AU2002359436A1 (en) 2001-11-13 2003-06-23 Rubicon Genomics Inc. Dna amplification and sequencing using dna molecules generated by random fragmentation
DE10214232A1 (de) 2002-03-25 2003-10-23 Epigenomics Ag Verfahren und Vorrichtung für die DNA Methylierungsanalyse
US7563567B1 (en) * 2002-04-12 2009-07-21 The Curators of the Univeristy of Missouri of Columbia Use of ECIST microarrays in an integrated method for assessing DNA methylation, gene expression and histone acetylation
EP2159285B1 (en) * 2003-01-29 2012-09-26 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US20040209299A1 (en) * 2003-03-07 2004-10-21 Rubicon Genomics, Inc. In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA
JP4773338B2 (ja) * 2003-03-07 2011-09-14 ルビコン ゲノミクス, インコーポレイテッド Dna重合プロセスにより生成される全ゲノムおよび全トランスクリプトームライブラリーの増幅および分析
US20050009059A1 (en) 2003-05-07 2005-01-13 Affymetrix, Inc. Analysis of methylation status using oligonucleotide arrays
KR100545295B1 (ko) * 2003-09-29 2006-01-24 현대모비스 주식회사 차량용 조향장치의 너클 어셈블리
EP2380993B1 (en) 2004-03-08 2015-12-23 Rubicon Genomics, Inc. Method for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation
US10795667B2 (en) 2017-12-21 2020-10-06 Microsoft Technology Licensing, Llc Facilitating data type detection using existing code

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US20050202490A1 (en) 2005-09-15
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CA2559209C (en) 2016-06-07
CA2902980A1 (en) 2005-09-29
US9708652B2 (en) 2017-07-18
WO2005090607A1 (en) 2005-09-29
US20130309668A1 (en) 2013-11-21
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