ATE244771T1 - Verfahren zur erkennung von nukleinsäuremethylierungen durch aflp - Google Patents

Verfahren zur erkennung von nukleinsäuremethylierungen durch aflp

Info

Publication number
ATE244771T1
ATE244771T1 AT98202549T AT98202549T ATE244771T1 AT E244771 T1 ATE244771 T1 AT E244771T1 AT 98202549 T AT98202549 T AT 98202549T AT 98202549 T AT98202549 T AT 98202549T AT E244771 T1 ATE244771 T1 AT E244771T1
Authority
AT
Austria
Prior art keywords
aflp
methylated
generating
dna
fingerprint
Prior art date
Application number
AT98202549T
Other languages
English (en)
Inventor
Marnik Johan Roger Vuylsteke
Petrus Antonius Josephina Vos
Marcus Florent Oscar Zabeau
Original Assignee
Keygene Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Keygene Nv filed Critical Keygene Nv
Application granted granted Critical
Publication of ATE244771T1 publication Critical patent/ATE244771T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
AT98202549T 1998-07-29 1998-07-29 Verfahren zur erkennung von nukleinsäuremethylierungen durch aflp ATE244771T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP98202549A EP0976835B1 (de) 1998-07-29 1998-07-29 Verfahren zur Erkennung von Nukleinsäuremethylierungen durch AFLP

Publications (1)

Publication Number Publication Date
ATE244771T1 true ATE244771T1 (de) 2003-07-15

Family

ID=8233987

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98202549T ATE244771T1 (de) 1998-07-29 1998-07-29 Verfahren zur erkennung von nukleinsäuremethylierungen durch aflp

Country Status (4)

Country Link
US (1) US6300071B1 (de)
EP (1) EP0976835B1 (de)
AT (1) ATE244771T1 (de)
DE (1) DE69816286T2 (de)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6605432B1 (en) * 1999-02-05 2003-08-12 Curators Of The University Of Missouri High-throughput methods for detecting DNA methylation
AU2308900A (en) * 1999-02-05 2000-08-25 Amersham Pharmacia Biotech Uk Limited Analysis method
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
WO2001090415A2 (en) 2000-05-20 2001-11-29 The Regents Of The University Of Michigan Method of producing a dna library using positional amplification
CA2410317A1 (en) * 2000-05-22 2001-11-29 The Johns Hopkins University Methods for assaying gene imprinting and methylated cpg islands
WO2002037398A2 (en) * 2000-11-02 2002-05-10 Epigenomics Ag Systems, methods and computer program products for guiding the selection of therapeutic treatment regimens
US20030143554A1 (en) * 2001-03-31 2003-07-31 Berres Mark E. Method of genotyping by determination of allele copy number
AU2002318336A1 (en) * 2001-06-08 2002-12-23 U.S. Genomics, Inc. Methods and products for analyzing nucleic acids based on methylation status
US20040197791A1 (en) * 2001-06-29 2004-10-07 Makarov Vladimir L. Methods of using nick translate libraries for snp analysis
AU2002341891A1 (en) * 2001-09-26 2003-04-07 Epigenx Pharmaceuticals, Inc. Assays for dna methylation changes
WO2003035860A1 (en) * 2001-10-24 2003-05-01 The Trustees Of Columbia University In The City Of New York A method for gene identification based on differential dna methylation
US20030232371A1 (en) * 2001-10-24 2003-12-18 Bestor Timothy H. Methods for detecting methylated promoters based on differential DNA methylation
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
EP1470255A2 (de) * 2002-01-30 2004-10-27 Epigenomics AG Nachweis des zelldifferenzierungszustandes mittels methylierungsmuster
EP1497447A4 (de) 2002-04-12 2006-09-20 Univ Missouri Ecist-mikroarrays für doppel-screening von dna-hypermethylierung und gen-silencing und zur verwendung in einem integrierten system zur beurteilung der genexpression, dna-methylierung und histon-acetylierung
ATE462017T1 (de) * 2002-12-02 2010-04-15 Illumina Cambridge Ltd Bestimmung der methylierung von nukleinsäuresequenzen
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
US20050009059A1 (en) * 2003-05-07 2005-01-13 Affymetrix, Inc. Analysis of methylation status using oligonucleotide arrays
US20050032102A1 (en) * 2003-07-22 2005-02-10 Affymetrix, Inc. Mapping genomic rearrangements
WO2005012575A1 (en) * 2003-08-01 2005-02-10 U.S. Genomics, Inc. Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions
DE10353419B3 (de) * 2003-11-09 2005-06-30 Epigenomics Ag Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA-Sequenzen mittels hemimethylierungssensitiver Restriktionsenzyme
US8440404B2 (en) 2004-03-08 2013-05-14 Rubicon Genomics Methods and compositions for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation
US7364855B2 (en) 2004-04-30 2008-04-29 Applera Corporation Methods and kits for methylation detection
EP1632578A1 (de) 2004-09-03 2006-03-08 Roche Diagnostics GmbH Methode zur Dekontamination der DNA
US20090111096A1 (en) * 2004-10-06 2009-04-30 National Institute Of Radiological Science Method of exhaustive analysis of transcriptionally-active domain (non-methylated domain) on genome
US20060134650A1 (en) * 2004-12-21 2006-06-22 Illumina, Inc. Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis
EP1910562B1 (de) 2005-06-23 2010-12-08 Keygene N.V. Strategien mit hohem durchsatz zur identifizierung und zum nachweis von polymorphismen
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
WO2007018602A1 (en) 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Isolation of cpg islands by thermal segregation and enzymatic selection-amplification method
DK1924704T3 (da) 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion
CA2910861C (en) 2005-09-29 2018-08-07 Michael Josephus Theresia Van Eijk High throughput screening of mutagenized populations
US10316364B2 (en) 2005-09-29 2019-06-11 Keygene N.V. Method for identifying the source of an amplicon
ES2882401T3 (es) 2005-12-22 2021-12-01 Keygene Nv Método de detección de polimorfismos basado en AFLP de alto rendimiento
WO2007073221A1 (en) 2005-12-22 2007-06-28 Instytut Hodowli I Aklimatyzacji Roslin (Plant Breeding And Acclimatization Institute) A method of identifying induced variability in in vitro cultures
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
EP2002017B1 (de) 2006-04-04 2015-06-10 Keygene N.V. Nachweis von molekularmarkern mit hohem durchsatz auf der basis von restriktionsfragmenten
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
WO2008111453A1 (ja) * 2007-03-07 2008-09-18 The University Of Tokyo Dna断片の増幅方法
US8642294B2 (en) * 2007-06-20 2014-02-04 Albert Einstein College Of Medicine Of Yeshiva University Methods for determining cytosine methylation in DNA and uses thereof
US8541207B2 (en) 2008-10-22 2013-09-24 Illumina, Inc. Preservation of information related to genomic DNA methylation
US8394584B2 (en) 2008-12-19 2013-03-12 The Board Of Trustees Of The University Of Illinois Detecting and sorting methylated DNA using a synthetic nanopore
US20100273164A1 (en) * 2009-03-24 2010-10-28 President And Fellows Of Harvard College Targeted and Whole-Genome Technologies to Profile DNA Cytosine Methylation
US8748091B2 (en) * 2009-12-18 2014-06-10 The Board Of Trustees Of The University Of Illinois Characterizing stretched polynucleotides in a synthetic nanopassage
WO2012162139A1 (en) 2011-05-20 2012-11-29 The Regents Of The University Of California Method to estimate age of individual based on epigenetic markers in biological sample
US10058306B2 (en) 2012-06-22 2018-08-28 Preprogen, LLC Method for obtaining fetal cells and fetal cellular components
SG11202107670TA (en) 2019-01-18 2021-08-30 Univ California Dna methylation measurement for mammals based on conserved loci
WO2023175019A1 (en) 2022-03-15 2023-09-21 Genknowme S.A. Method determining the difference between the biological age and the chronological age of a subject

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL103267A (en) * 1991-09-24 2004-07-25 Keygene Nv Process and kit for amplification of restriction fragment obtained from starting dna
US5843649A (en) * 1995-03-01 1998-12-01 University Of Colorado Method of identifying clonal cell samples using heteroduplex generators
US5871917A (en) * 1996-05-31 1999-02-16 North Shore University Hospital Research Corp. Identification of differentially methylated and mutated nucleic acids
AU4174397A (en) * 1996-08-30 1998-03-19 Life Technologies, Inc. Methods for identification and isolation of specific nucleotide sequences in cdna and genomic dna

Also Published As

Publication number Publication date
DE69816286T2 (de) 2004-05-27
DE69816286D1 (de) 2003-08-14
EP0976835B1 (de) 2003-07-09
US6300071B1 (en) 2001-10-09
EP0976835A1 (de) 2000-02-02

Similar Documents

Publication Publication Date Title
ATE244771T1 (de) Verfahren zur erkennung von nukleinsäuremethylierungen durch aflp
ATE326549T1 (de) Spezifische bestimmung der methylierung
DE69719057T2 (de) Verfahren zur signalverstärkung
DE60125715D1 (de) Verfahren und testsysteme zur identifizierung von elite event gat-zm1 in biologischen proben
ATE551656T1 (de) Verfahren und vorrichtung zum identifizieren von neuem media-inhalt
DE69735445D1 (de) Abspaltbare, nicht-flüchtige moleküle zur massenmarkierung
DK1042664T3 (da) Fremgangsmåde til påvisning af reaktioner ved hjælp af koincidensanalyse
DE69941044D1 (de) Methoden, Apparat und Computerprogramm zur quantitativen Bestimmung von Nukleinsäurensequenzen in Proben unter Verwendung von Standardkurven und Abschätzungen von Amplifikationsverhältnissen
ATE278805T1 (de) Verfahren zur quantifizierung von cholesterol in lipoproteinen und quantifizierungsreagenzien
ATE353455T1 (de) Verfahren zur sequenzanalyse
DE60029323D1 (de) Verfahren zur analyse der dna-methylierung mit hoher durchsatzrate
WO2002031190A3 (en) Methods of identification and isolation of polynucleotides containing nucleic acid differences
ATE230801T1 (de) Verfahren zur detektion von zielnukleinsäure, verfahren zu ihrer quantifizierung und pyrylium- verbindungen zur chemilumineszenz-analyse
ATE431428T1 (de) Ein verfahren zur analyse von polynukleotiden
DE50214185D1 (de) Verfahren zur analyse genomischer methylierungsmuster
WO2003085374A3 (en) Multiplexed assays using electrophoretically separated molecular tags
DE60130970D1 (de) Reagenziensatz zur detektion von mikroorganismen, vorrichtung zur quantifizierungvon mikroorganismen und verfahren zur quantifizierung von mikroorganismen
DE50110172D1 (de) Verfahren zur altersbestimmung von individuen
DE60131680D1 (de) Verfahren und vorrichtung zum identifizieren einer biologischen probe
DE50208262D1 (de) Verfahren zur bestimmung von flüchtigen substanzen in lösung
WO2001007650A3 (en) Improvements in and relating to forensic investigations
ATE274702T1 (de) Verwendung derivatisierter reagenzien in chemilumineszenz- und elektrochemilumineszenz- nachweisverfahren
DE69841783D1 (de) Verfahren zur Bestimmung des Histocompatibiltätsantigenlokus Klasse II
DE69713754T2 (de) Verfahren zur analyse von blutproben
DE59906298D1 (de) Verfahren und vorrichtung zur quantifizierung von dna und rna

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties