DE69816286D1 - Verfahren zur Erkennung von Nukleinsäuremethylierungen durch AFLP - Google Patents

Verfahren zur Erkennung von Nukleinsäuremethylierungen durch AFLP

Info

Publication number
DE69816286D1
DE69816286D1 DE69816286T DE69816286T DE69816286D1 DE 69816286 D1 DE69816286 D1 DE 69816286D1 DE 69816286 T DE69816286 T DE 69816286T DE 69816286 T DE69816286 T DE 69816286T DE 69816286 D1 DE69816286 D1 DE 69816286D1
Authority
DE
Germany
Prior art keywords
aflp
methylated
generating
dna
fingerprint
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69816286T
Other languages
English (en)
Other versions
DE69816286T2 (de
Inventor
Marnik Johan Roger Vuylsteke
Petrus Antonius Josephina Vos
Marcus Florent Oscar Zabeau
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Keygene NV
Original Assignee
Keygene NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Keygene NV filed Critical Keygene NV
Application granted granted Critical
Publication of DE69816286D1 publication Critical patent/DE69816286D1/de
Publication of DE69816286T2 publication Critical patent/DE69816286T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69816286T 1998-07-29 1998-07-29 Verfahren zur Erkennung von Nukleinsäuremethylierungen durch AFLP Expired - Fee Related DE69816286T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP98202549A EP0976835B1 (de) 1998-07-29 1998-07-29 Verfahren zur Erkennung von Nukleinsäuremethylierungen durch AFLP

Publications (2)

Publication Number Publication Date
DE69816286D1 true DE69816286D1 (de) 2003-08-14
DE69816286T2 DE69816286T2 (de) 2004-05-27

Family

ID=8233987

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69816286T Expired - Fee Related DE69816286T2 (de) 1998-07-29 1998-07-29 Verfahren zur Erkennung von Nukleinsäuremethylierungen durch AFLP

Country Status (4)

Country Link
US (1) US6300071B1 (de)
EP (1) EP0976835B1 (de)
AT (1) ATE244771T1 (de)
DE (1) DE69816286T2 (de)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6605432B1 (en) 1999-02-05 2003-08-12 Curators Of The University Of Missouri High-throughput methods for detecting DNA methylation
JP2002535999A (ja) * 1999-02-05 2002-10-29 アマシャム バイオサイエンス ユーケイ リミテッド ゲノム分析方法
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US6828098B2 (en) 2000-05-20 2004-12-07 The Regents Of The University Of Michigan Method of producing a DNA library using positional amplification based on the use of adaptors and nick translation
WO2001090313A2 (en) * 2000-05-22 2001-11-29 The Johns Hopkins University METHODS FOR ASSAYING GENE IMPRINTING AND METHYLATED CpG ISLANDS
EP1358616A2 (de) * 2000-11-02 2003-11-05 Epigenomics AG Systeme, verfahren und computerprogrammprodukte zum anleiten der auswahl einer therapeutischen behandlungsweise auf der grundlage des methylationsstatus der dna
US20030143554A1 (en) * 2001-03-31 2003-07-31 Berres Mark E. Method of genotyping by determination of allele copy number
EP1402071A4 (de) * 2001-06-08 2005-12-14 Us Genomics Inc Verfahren und produkte zur analyse von nukleinsäuren auf grundlage des methylierungsstatus
US20040197791A1 (en) * 2001-06-29 2004-10-07 Makarov Vladimir L. Methods of using nick translate libraries for snp analysis
WO2003027259A2 (en) * 2001-09-26 2003-04-03 Epigenx Pharmaceuticals, Inc. Assays for dna methylation changes
US20030099997A1 (en) * 2001-10-24 2003-05-29 Bestor Timothy H. Method for gene identification based on differential DNA methylation
US20030232371A1 (en) * 2001-10-24 2003-12-18 Bestor Timothy H. Methods for detecting methylated promoters based on differential DNA methylation
US20110151438A9 (en) * 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
EP1470255A2 (de) * 2002-01-30 2004-10-27 Epigenomics AG Nachweis des zelldifferenzierungszustandes mittels methylierungsmuster
EP1497447A4 (de) 2002-04-12 2006-09-20 Univ Missouri Ecist-mikroarrays für doppel-screening von dna-hypermethylierung und gen-silencing und zur verwendung in einem integrierten system zur beurteilung der genexpression, dna-methylierung und histon-acetylierung
ATE462017T1 (de) * 2002-12-02 2010-04-15 Illumina Cambridge Ltd Bestimmung der methylierung von nukleinsäuresequenzen
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
US20050009059A1 (en) * 2003-05-07 2005-01-13 Affymetrix, Inc. Analysis of methylation status using oligonucleotide arrays
US20050032102A1 (en) * 2003-07-22 2005-02-10 Affymetrix, Inc. Mapping genomic rearrangements
WO2005012575A1 (en) * 2003-08-01 2005-02-10 U.S. Genomics, Inc. Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions
DE10353419B3 (de) * 2003-11-09 2005-06-30 Epigenomics Ag Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA-Sequenzen mittels hemimethylierungssensitiver Restriktionsenzyme
CA2902980A1 (en) 2004-03-08 2005-09-29 Rubicon Genomics, Inc. Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation
WO2005108618A2 (en) * 2004-04-30 2005-11-17 Applera Corporation Methods and kits for methylation detection
EP1632578A1 (de) 2004-09-03 2006-03-08 Roche Diagnostics GmbH Methode zur Dekontamination der DNA
WO2006038416A1 (ja) * 2004-10-06 2006-04-13 National Institute Of Radiological Sciences ゲノム上の転写活性領域（非メチル化領域）の網羅的解析法
US20060134650A1 (en) * 2004-12-21 2006-06-22 Illumina, Inc. Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis
EP2292788B1 (de) 2005-06-23 2012-05-09 Keygene N.V. Strategien für eine hohe Durchsatzidentifikation und Erkennung von Polymorphismen
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
DK1924704T3 (da) 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion
EP1924705A1 (de) 2005-08-02 2008-05-28 Rubicon Genomics, Inc. Isolierung von cpg-inseln durch thermische segregation und enzymatisches selektions-amplifikations-verfahren
DK1929039T4 (en) 2005-09-29 2014-02-17 Keygene Nv High throughput-screening af mutageniserede populationer
US10316364B2 (en) 2005-09-29 2019-06-11 Keygene N.V. Method for identifying the source of an amplicon
EP3404114B1 (de) 2005-12-22 2021-05-05 Keygene N.V. Verfahren zur auf aflp basierenden hochdurchsatz-polymorphismusdetektion
AU2005339395B2 (en) * 2005-12-22 2012-08-16 Instytut Hodowli I Aklimatyzacji Roslin (Plant Breeding And Acclimatization Institute) A method of identifying induced variability in in vitro cultures
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
EP3239304B1 (de) 2006-04-04 2020-08-19 Keygene N.V. Nachweis von molekularmarkern mit hohem durchsatz auf der basis von aflp und mit hoher durchsatzsequenzierung
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
WO2008111453A1 (ja) * 2007-03-07 2008-09-18 The University Of Tokyo Dna断片の増幅方法
US8642294B2 (en) * 2007-06-20 2014-02-04 Albert Einstein College Of Medicine Of Yeshiva University Methods for determining cytosine methylation in DNA and uses thereof
EP2340314B8 (de) 2008-10-22 2015-02-18 Illumina, Inc. Erhaltung von informationen in verbindung mit genomischer dna-methylierung
WO2010080617A2 (en) 2008-12-19 2010-07-15 The Board Of Trustees Of The University Of Illinois Detecting and sorting methylated dna using a synthetic nanopore
US20100273164A1 (en) * 2009-03-24 2010-10-28 President And Fellows Of Harvard College Targeted and Whole-Genome Technologies to Profile DNA Cytosine Methylation
US8748091B2 (en) * 2009-12-18 2014-06-10 The Board Of Trustees Of The University Of Illinois Characterizing stretched polynucleotides in a synthetic nanopassage
US10435743B2 (en) 2011-05-20 2019-10-08 The Regents Of The University Of California Method to estimate age of individual based on epigenetic markers in biological sample
US10058306B2 (en) 2012-06-22 2018-08-28 Preprogen, LLC Method for obtaining fetal cells and fetal cellular components
JP7320067B2 (ja) 2019-01-18 2023-08-02 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 保存された遺伝子座に基づく、哺乳動物に関するｄｎａメチル化測定
WO2023175019A1 (en) 2022-03-15 2023-09-21 Genknowme S.A. Method determining the difference between the biological age and the chronological age of a subject

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100321510B1 (ko) * 1991-09-24 2005-01-10 키진 엔.브이. 선택적인 제한단편의 증폭:디엔에이(dna) 핑거프린트법
US5843649A (en) * 1995-03-01 1998-12-01 University Of Colorado Method of identifying clonal cell samples using heteroduplex generators
US5871917A (en) * 1996-05-31 1999-02-16 North Shore University Hospital Research Corp. Identification of differentially methylated and mutated nucleic acids
AU4174397A (en) * 1996-08-30 1998-03-19 Life Technologies, Inc. Methods for identification and isolation of specific nucleotide sequences in cdna and genomic dna

Also Published As

Publication number Publication date
US6300071B1 (en) 2001-10-09
EP0976835B1 (de) 2003-07-09
DE69816286T2 (de) 2004-05-27
EP0976835A1 (de) 2000-02-02
ATE244771T1 (de) 2003-07-15

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Legal Events

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8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee