FR2989089B1 - Oligonucleotides modifies comprenant des fonctions thiol et leur utilisation pour la detection d'acides nucleiques - Google Patents

Oligonucleotides modifies comprenant des fonctions thiol et leur utilisation pour la detection d'acides nucleiques Download PDF

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Publication number
FR2989089B1
FR2989089B1 FR1253122A FR1253122A FR2989089B1 FR 2989089 B1 FR2989089 B1 FR 2989089B1 FR 1253122 A FR1253122 A FR 1253122A FR 1253122 A FR1253122 A FR 1253122A FR 2989089 B1 FR2989089 B1 FR 2989089B1
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Prior art keywords
carbon
functions
detection
nucleic acids
relates
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Active
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FR1253122A
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English (en)
French (fr)
Other versions
FR2989089A1 (fr
Inventor
Chantal Fournier Wirth
Myriam Lereau
Jean Francois Cantaloube
Jean Jacques Vasseur
Francois Morvan
Albert Meyer
Julie Mayen
Carole Chaix
Carole Farre
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Etablissement Francais du Sang
Universite de Montpellier
Universite Claude Bernard Lyon 1
Original Assignee
Centre National de la Recherche Scientifique CNRS
Etablissement Francais du Sang
Universite de Montpellier
Universite Claude Bernard Lyon 1
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Priority to FR1253122A priority Critical patent/FR2989089B1/fr
Application filed by Centre National de la Recherche Scientifique CNRS, Etablissement Francais du Sang, Universite de Montpellier, Universite Claude Bernard Lyon 1 filed Critical Centre National de la Recherche Scientifique CNRS
Priority to JP2015503888A priority patent/JP6175489B2/ja
Priority to BR112014024601-7A priority patent/BR112014024601B1/pt
Priority to US14/390,748 priority patent/US20150232957A1/en
Priority to ES13716756.5T priority patent/ES2624993T3/es
Priority to EP13716756.5A priority patent/EP2859008B1/fr
Priority to AU2013244900A priority patent/AU2013244900B2/en
Priority to IN8170DEN2014 priority patent/IN2014DN08170A/en
Priority to KR1020147027987A priority patent/KR102186143B1/ko
Priority to PCT/EP2013/057150 priority patent/WO2013150122A1/fr
Priority to EA201491821A priority patent/EA029907B1/ru
Priority to CA2868926A priority patent/CA2868926C/fr
Publication of FR2989089A1 publication Critical patent/FR2989089A1/fr
Priority to US16/271,581 priority patent/US11390643B2/en
Application granted granted Critical
Publication of FR2989089B1 publication Critical patent/FR2989089B1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Communicable Diseases (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
FR1253122A 2012-04-04 2012-04-04 Oligonucleotides modifies comprenant des fonctions thiol et leur utilisation pour la detection d'acides nucleiques Active FR2989089B1 (fr)

Priority Applications (13)

Application Number Priority Date Filing Date Title
FR1253122A FR2989089B1 (fr) 2012-04-04 2012-04-04 Oligonucleotides modifies comprenant des fonctions thiol et leur utilisation pour la detection d'acides nucleiques
EA201491821A EA029907B1 (ru) 2012-04-04 2013-04-04 Модифицированные олигонуклеотиды, включающие тиольные функциональные группы, и их применение для детекции нуклеиновых кислот
US14/390,748 US20150232957A1 (en) 2012-04-04 2013-04-04 Modified oligonucleotides comprising thiol functions and use thereof for detecting nucleic acids
ES13716756.5T ES2624993T3 (es) 2012-04-04 2013-04-04 Oligonucleótidos modificados que comprenden funciones tiol y su uso para la detección de ácidos nucleicos
EP13716756.5A EP2859008B1 (fr) 2012-04-04 2013-04-04 Oligonucleotides modifies compenant des fonctions thiol et leur utilisation pour la detection d'acides nucleiques
AU2013244900A AU2013244900B2 (en) 2012-04-04 2013-04-04 Modified oligonucleotides comprising thiol functions and the use of same for the detection of nucleic acids
JP2015503888A JP6175489B2 (ja) 2012-04-04 2013-04-04 チオール官能基を含む修飾オリゴヌクレオチドおよび核酸検出のためのこれらの使用
KR1020147027987A KR102186143B1 (ko) 2012-04-04 2013-04-04 티올 관능기를 포함하는 개질된 올리고뉴클레오티드 및 이를 이용한 핵산의 검출
PCT/EP2013/057150 WO2013150122A1 (fr) 2012-04-04 2013-04-04 Oligonucleotides modifies compenant des fonctions thiol et leur utilisation pour la detection d'acides nucleiques
BR112014024601-7A BR112014024601B1 (pt) 2012-04-04 2013-04-04 Oligonucleotídeo modificado composto de funções tióis; substrato enxertado com oligonucleotídeo modificado; método e kit de detecção de ácido nucleico em uma amostra de oligonucleotídeo modificado; e o uso oligonucleotídeo modificado para a detecção de ácidos nucleicos
CA2868926A CA2868926C (fr) 2012-04-04 2013-04-04 Oligonucleotides modifies comprenant des fonctions thiol et leur utilisation pour la detection d'acides nucleiques
IN8170DEN2014 IN2014DN08170A (enExample) 2012-04-04 2013-04-04
US16/271,581 US11390643B2 (en) 2012-04-04 2019-02-08 Modified oligonucleotides comprising thiol functions and use thereof for detecting nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1253122 2012-04-04
FR1253122A FR2989089B1 (fr) 2012-04-04 2012-04-04 Oligonucleotides modifies comprenant des fonctions thiol et leur utilisation pour la detection d'acides nucleiques

Publications (2)

Publication Number Publication Date
FR2989089A1 FR2989089A1 (fr) 2013-10-11
FR2989089B1 true FR2989089B1 (fr) 2020-02-07

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FR1253122A Active FR2989089B1 (fr) 2012-04-04 2012-04-04 Oligonucleotides modifies comprenant des fonctions thiol et leur utilisation pour la detection d'acides nucleiques

Country Status (12)

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US (2) US20150232957A1 (enExample)
EP (1) EP2859008B1 (enExample)
JP (1) JP6175489B2 (enExample)
KR (1) KR102186143B1 (enExample)
AU (1) AU2013244900B2 (enExample)
BR (1) BR112014024601B1 (enExample)
CA (1) CA2868926C (enExample)
EA (1) EA029907B1 (enExample)
ES (1) ES2624993T3 (enExample)
FR (1) FR2989089B1 (enExample)
IN (1) IN2014DN08170A (enExample)
WO (1) WO2013150122A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017218293A1 (en) 2016-06-16 2017-12-21 Richard Edward Watts Oligonucleotide directed and recorded combinatorial synthesis of encoded probe molecules
WO2018204420A1 (en) 2017-05-02 2018-11-08 Haystack Sciences Corporation Molecules for verifying oligonucleotide directed combinatorial synthesis and methods of making and using the same
JP7282379B2 (ja) * 2017-08-22 2023-05-29 国立大学法人東海国立大学機構 修飾ポリヌクレオチド
US20200263163A1 (en) * 2017-09-25 2020-08-20 Haystack Sciences Corporation Multinomial encoding for oligonucleotide-directed combinatorial chemistry
EP4305198A1 (en) 2021-03-09 2024-01-17 Etablissement Français du Sang Detection of infectious agent based on recombinase polymerase amplification combined with a magnetic field-enhanced agglutination

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6041183A (ja) * 1983-08-16 1985-03-04 三菱電機株式会社 入場管理装置
IE922292A1 (en) * 1991-07-15 1993-01-27 Jolla Pharma Modified phosphorous intermediates for providing functional¹groups on the 5' end of oligonucleotides
JPH0641183A (ja) * 1992-07-23 1994-02-15 Mitsubishi Kasei Corp オリゴヌクレオチド単分子膜
DE10163836A1 (de) * 2001-12-22 2003-07-10 Friz Biochem Gmbh Multifunktionales Reagenz zur Synthese von thiolmodifizierten Oligomeren
US7371585B2 (en) * 2003-12-31 2008-05-13 Genencor International, Inc. Membranes incorporating recognition moieties
US7167395B1 (en) 2005-07-13 2007-01-23 Infineon Technologies Flash Gmbh & Co. Kg Non-volatile semiconductor memory
AU2006330464A1 (en) 2005-12-23 2007-07-05 Siemens Healthcare Diagnostics Inc. Methods and reagents for genotyping HCV
FR2989086B1 (fr) 2012-04-04 2017-03-24 Centre Nat De La Rech Scient (Cnrs) Composes thiol et leur utilisation pour la synthese d'oligonucleotides modifies

Also Published As

Publication number Publication date
CA2868926A1 (fr) 2013-10-10
US20190376151A1 (en) 2019-12-12
ES2624993T3 (es) 2017-07-18
FR2989089A1 (fr) 2013-10-11
BR112014024601B1 (pt) 2022-05-24
JP2015514402A (ja) 2015-05-21
KR102186143B1 (ko) 2020-12-03
IN2014DN08170A (enExample) 2015-05-01
JP6175489B2 (ja) 2017-08-02
KR20150021493A (ko) 2015-03-02
EA201491821A1 (ru) 2015-02-27
CA2868926C (fr) 2021-05-04
EA029907B1 (ru) 2018-05-31
EP2859008B1 (fr) 2017-02-22
US11390643B2 (en) 2022-07-19
BR112014024601A2 (pt) 2018-04-17
US20150232957A1 (en) 2015-08-20
AU2013244900A1 (en) 2014-10-16
AU2013244900B2 (en) 2017-01-12
EP2859008A1 (fr) 2015-04-15
WO2013150122A1 (fr) 2013-10-10

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