FR2461007A1 - Procede et reactif pour le dosage de la lipase dans les liquides biologiques - Google Patents
Procede et reactif pour le dosage de la lipase dans les liquides biologiques Download PDFInfo
- Publication number
- FR2461007A1 FR2461007A1 FR8015088A FR8015088A FR2461007A1 FR 2461007 A1 FR2461007 A1 FR 2461007A1 FR 8015088 A FR8015088 A FR 8015088A FR 8015088 A FR8015088 A FR 8015088A FR 2461007 A1 FR2461007 A1 FR 2461007A1
- Authority
- FR
- France
- Prior art keywords
- reagent
- lipase
- color
- inhibitor
- cholinesterase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 50
- 108090001060 Lipase Proteins 0.000 title claims abstract description 43
- 102000004882 Lipase Human genes 0.000 title claims abstract description 43
- 239000004367 Lipase Substances 0.000 title claims abstract description 43
- 235000019421 lipase Nutrition 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 33
- 230000008569 process Effects 0.000 title claims abstract description 23
- 238000003556 assay Methods 0.000 title claims description 11
- 239000007788 liquid Substances 0.000 title abstract description 5
- 239000000758 substrate Substances 0.000 claims abstract description 28
- 239000003112 inhibitor Substances 0.000 claims abstract description 24
- 108010053652 Butyrylcholinesterase Proteins 0.000 claims abstract description 22
- 238000011534 incubation Methods 0.000 claims abstract description 20
- 239000003774 sulfhydryl reagent Substances 0.000 claims abstract description 20
- 239000002736 nonionic surfactant Substances 0.000 claims abstract description 16
- 102000009027 Albumins Human genes 0.000 claims abstract description 12
- 108010088751 Albumins Proteins 0.000 claims abstract description 12
- 150000003839 salts Chemical class 0.000 claims abstract description 11
- 239000013060 biological fluid Substances 0.000 claims abstract description 10
- HZOTZTANVBDFOF-PBCQUBLHSA-N physostigmine salicylate Chemical compound OC(=O)C1=CC=CC=C1O.C12=CC(OC(=O)NC)=CC=C2N(C)[C@@H]2[C@@]1(C)CCN2C HZOTZTANVBDFOF-PBCQUBLHSA-N 0.000 claims abstract description 5
- 239000007787 solid Substances 0.000 claims abstract description 5
- 239000000126 substance Substances 0.000 claims abstract description 5
- 239000000203 mixture Substances 0.000 claims description 42
- 102100032404 Cholinesterase Human genes 0.000 claims description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 239000000243 solution Substances 0.000 claims description 19
- 108090000322 Cholinesterases Proteins 0.000 claims description 17
- 150000001875 compounds Chemical class 0.000 claims description 14
- 230000000694 effects Effects 0.000 claims description 13
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 229940048961 cholinesterase Drugs 0.000 claims description 12
- PIJVFDBKTWXHHD-HIFRSBDPSA-N physostigmine Chemical group C12=CC(OC(=O)NC)=CC=C2N(C)[C@@H]2[C@@]1(C)CCN2C PIJVFDBKTWXHHD-HIFRSBDPSA-N 0.000 claims description 12
- PIJVFDBKTWXHHD-UHFFFAOYSA-N Physostigmine Natural products C12=CC(OC(=O)NC)=CC=C2N(C)C2C1(C)CCN2C PIJVFDBKTWXHHD-UHFFFAOYSA-N 0.000 claims description 11
- 239000000872 buffer Substances 0.000 claims description 10
- 102000003914 Cholinesterases Human genes 0.000 claims description 9
- 229960001697 physostigmine Drugs 0.000 claims description 9
- 235000019626 lipase activity Nutrition 0.000 claims description 8
- 230000003381 solubilizing effect Effects 0.000 claims description 5
- 239000004094 surface-active agent Substances 0.000 claims description 5
- 125000000217 alkyl group Chemical group 0.000 claims description 4
- 108010051152 Carboxylesterase Proteins 0.000 claims description 3
- 102000013392 Carboxylesterase Human genes 0.000 claims description 3
- 108090000371 Esterases Proteins 0.000 claims description 3
- 125000004103 aminoalkyl group Chemical group 0.000 claims description 3
- 239000000544 cholinesterase inhibitor Substances 0.000 claims description 3
- 230000002349 favourable effect Effects 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 3
- 239000000693 micelle Substances 0.000 claims description 3
- 102000004195 Isomerases Human genes 0.000 claims description 2
- 108090000769 Isomerases Proteins 0.000 claims description 2
- 239000003929 acidic solution Substances 0.000 claims description 2
- 239000012442 inert solvent Substances 0.000 claims description 2
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 claims 3
- 229940122041 Cholinesterase inhibitor Drugs 0.000 claims 2
- 125000000022 2-aminoethyl group Chemical group [H]C([*])([H])C([H])([H])N([H])[H] 0.000 claims 1
- ZNQVEEAIQZEUHB-UHFFFAOYSA-N 2-ethoxyethanol Chemical compound CCOCCO ZNQVEEAIQZEUHB-UHFFFAOYSA-N 0.000 claims 1
- 150000001720 carbohydrates Chemical class 0.000 claims 1
- ZYCMDWDFIQDPLP-UHFFFAOYSA-N hbr bromine Chemical compound Br.Br ZYCMDWDFIQDPLP-UHFFFAOYSA-N 0.000 claims 1
- 230000000415 inactivating effect Effects 0.000 claims 1
- -1 SULPHYDRYL Chemical class 0.000 abstract description 6
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 102000021944 Butyrylcholinesterase Human genes 0.000 abstract 1
- 238000002835 absorbance Methods 0.000 description 23
- 210000002966 serum Anatomy 0.000 description 20
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 14
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 12
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 9
- KIUMMUBSPKGMOY-UHFFFAOYSA-N 3,3'-Dithiobis(6-nitrobenzoic acid) Chemical compound C1=C([N+]([O-])=O)C(C(=O)O)=CC(SSC=2C=C(C(=CC=2)[N+]([O-])=O)C(O)=O)=C1 KIUMMUBSPKGMOY-UHFFFAOYSA-N 0.000 description 9
- 239000012153 distilled water Substances 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- YGSDEFSMJLZEOE-UHFFFAOYSA-M salicylate Chemical compound OC1=CC=CC=C1C([O-])=O YGSDEFSMJLZEOE-UHFFFAOYSA-M 0.000 description 9
- 229960001860 salicylate Drugs 0.000 description 9
- 230000002401 inhibitory effect Effects 0.000 description 7
- 229920002307 Dextran Polymers 0.000 description 6
- 239000002253 acid Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 6
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 5
- 239000007983 Tris buffer Substances 0.000 description 5
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 5
- 239000012086 standard solution Substances 0.000 description 5
- 125000003396 thiol group Chemical group [H]S* 0.000 description 5
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical class NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000003945 anionic surfactant Substances 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 238000004108 freeze drying Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- 102100033639 Acetylcholinesterase Human genes 0.000 description 3
- 108010022752 Acetylcholinesterase Proteins 0.000 description 3
- 229940022698 acetylcholinesterase Drugs 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000011541 reaction mixture Substances 0.000 description 3
- 239000003381 stabilizer Substances 0.000 description 3
- 231100000331 toxic Toxicity 0.000 description 3
- 230000002588 toxic effect Effects 0.000 description 3
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 3
- PMNLUUOXGOOLSP-UHFFFAOYSA-N 2-mercaptopropanoic acid Chemical compound CC(S)C(O)=O PMNLUUOXGOOLSP-UHFFFAOYSA-N 0.000 description 2
- 229940122274 Carboxylesterase inhibitor Drugs 0.000 description 2
- KRHYYFGTRYWZRS-UHFFFAOYSA-N Fluorane Chemical compound F KRHYYFGTRYWZRS-UHFFFAOYSA-N 0.000 description 2
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 2
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 2
- XDVMCVGTDUKDHL-UHFFFAOYSA-N [amino(2-azaniumylethylsulfanyl)methylidene]azanium;dibromide Chemical compound Br.Br.NCCSC(N)=N XDVMCVGTDUKDHL-UHFFFAOYSA-N 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 239000012445 acidic reagent Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- 108010009043 arylesterase Proteins 0.000 description 2
- 102000028848 arylesterase Human genes 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 229960002362 neostigmine Drugs 0.000 description 2
- LULNWZDBKTWDGK-UHFFFAOYSA-M neostigmine bromide Chemical compound [Br-].CN(C)C(=O)OC1=CC=CC([N+](C)(C)C)=C1 LULNWZDBKTWDGK-UHFFFAOYSA-M 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- UYDLBVPAAFVANX-UHFFFAOYSA-N octylphenoxy polyethoxyethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(OCCOCCOCCOCCO)C=C1 UYDLBVPAAFVANX-UHFFFAOYSA-N 0.000 description 2
- 125000004430 oxygen atom Chemical group O* 0.000 description 2
- 229920000136 polysorbate Polymers 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 229910052717 sulfur Inorganic materials 0.000 description 2
- 125000004434 sulfur atom Chemical group 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- WGJCBBASTRWVJL-UHFFFAOYSA-N 1,3-thiazolidine-2-thione Chemical compound SC1=NCCS1 WGJCBBASTRWVJL-UHFFFAOYSA-N 0.000 description 1
- KWVPFECTOKLOBL-KTKRTIGZSA-N 2-[(z)-octadec-9-enoxy]ethanol Chemical compound CCCCCCCC\C=C/CCCCCCCCOCCO KWVPFECTOKLOBL-KTKRTIGZSA-N 0.000 description 1
- HQAPREJURVMGMB-UHFFFAOYSA-M 2-acetylsulfanylethyl(trimethyl)azanium;bromide Chemical compound [Br-].CC(=O)SCC[N+](C)(C)C HQAPREJURVMGMB-UHFFFAOYSA-M 0.000 description 1
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 description 1
- 241000238876 Acari Species 0.000 description 1
- 101710150365 Albumin-1 Proteins 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 description 1
- 239000001828 Gelatine Substances 0.000 description 1
- 239000005949 Malathion Substances 0.000 description 1
- 206010033645 Pancreatitis Diseases 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- VNYBTNPBYXSMOO-UHFFFAOYSA-M Pyridostigmine bromide Chemical compound [Br-].CN(C)C(=O)OC1=CC=C[N+](C)=C1 VNYBTNPBYXSMOO-UHFFFAOYSA-M 0.000 description 1
- 239000004141 Sodium laurylsulphate Substances 0.000 description 1
- ABBQHOQBGMUPJH-UHFFFAOYSA-M Sodium salicylate Chemical compound [Na+].OC1=CC=CC=C1C([O-])=O ABBQHOQBGMUPJH-UHFFFAOYSA-M 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- RAHZWNYVWXNFOC-UHFFFAOYSA-N Sulphur dioxide Chemical group O=S=O RAHZWNYVWXNFOC-UHFFFAOYSA-N 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- DXUUXWKFVDVHIK-UHFFFAOYSA-N ambenonium chloride Chemical compound [Cl-].[Cl-].C=1C=CC=C(Cl)C=1C[N+](CC)(CC)CCNC(=O)C(=O)NCC[N+](CC)(CC)CC1=CC=CC=C1Cl DXUUXWKFVDVHIK-UHFFFAOYSA-N 0.000 description 1
- 229960004302 ambenonium chloride Drugs 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 239000011260 aqueous acid Substances 0.000 description 1
- 239000012062 aqueous buffer Substances 0.000 description 1
- 150000004657 carbamic acid derivatives Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229940125782 compound 2 Drugs 0.000 description 1
- JXSJBGJIGXNWCI-UHFFFAOYSA-N diethyl 2-[(dimethoxyphosphorothioyl)thio]succinate Chemical compound CCOC(=O)CC(SP(=S)(OC)OC)C(=O)OCC JXSJBGJIGXNWCI-UHFFFAOYSA-N 0.000 description 1
- MUCZHBLJLSDCSD-UHFFFAOYSA-N diisopropyl fluorophosphate Chemical compound CC(C)OP(F)(=O)OC(C)C MUCZHBLJLSDCSD-UHFFFAOYSA-N 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 125000000816 ethylene group Chemical group [H]C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- DWYMPOCYEZONEA-UHFFFAOYSA-L fluoridophosphate Chemical compound [O-]P([O-])(F)=O DWYMPOCYEZONEA-UHFFFAOYSA-L 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 229960000453 malathion Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- GLVAUDGFNGKCSF-UHFFFAOYSA-N mercaptopurine Chemical compound S=C1NC=NC2=C1NC=N2 GLVAUDGFNGKCSF-UHFFFAOYSA-N 0.000 description 1
- 229960001428 mercaptopurine Drugs 0.000 description 1
- OSZNNLWOYWAHSS-UHFFFAOYSA-M neostigmine methyl sulfate Chemical compound COS([O-])(=O)=O.CN(C)C(=O)OC1=CC=CC([N+](C)(C)C)=C1 OSZNNLWOYWAHSS-UHFFFAOYSA-M 0.000 description 1
- 229960002253 neostigmine methylsulfate Drugs 0.000 description 1
- LQNUZADURLCDLV-UHFFFAOYSA-N nitrobenzene Chemical compound [O-][N+](=O)C1=CC=CC=C1 LQNUZADURLCDLV-UHFFFAOYSA-N 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 210000001819 pancreatic juice Anatomy 0.000 description 1
- LCCNCVORNKJIRZ-UHFFFAOYSA-N parathion Chemical compound CCOP(=S)(OCC)OC1=CC=C([N+]([O-])=O)C=C1 LCCNCVORNKJIRZ-UHFFFAOYSA-N 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000008389 polyethoxylated castor oil Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- SSOLNOMRVKKSON-UHFFFAOYSA-N proguanil Chemical compound CC(C)\N=C(/N)N=C(N)NC1=CC=C(Cl)C=C1 SSOLNOMRVKKSON-UHFFFAOYSA-N 0.000 description 1
- 229960002151 pyridostigmine bromide Drugs 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000009666 routine test Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229960004025 sodium salicylate Drugs 0.000 description 1
- 239000012265 solid product Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000012089 stop solution Substances 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 229960000281 trometamol Drugs 0.000 description 1
- 125000005500 uronium group Chemical group 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/057,525 US4279994A (en) | 1979-07-13 | 1979-07-13 | Lipase determination method and reagent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2461007A1 true FR2461007A1 (fr) | 1981-01-30 |
| FR2461007B1 FR2461007B1 (2) | 1984-09-14 |
Family
ID=22011108
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR8015088A Granted FR2461007A1 (fr) | 1979-07-13 | 1980-07-07 | Procede et reactif pour le dosage de la lipase dans les liquides biologiques |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US4279994A (2) |
| JP (1) | JPS5615698A (2) |
| BE (1) | BE883387A (2) |
| CA (1) | CA1128843A (2) |
| DE (1) | DE3020072A1 (2) |
| DK (1) | DK221380A (2) |
| FI (1) | FI801799A7 (2) |
| FR (1) | FR2461007A1 (2) |
| GB (1) | GB2065881B (2) |
| IT (1) | IT1209237B (2) |
| NL (1) | NL8003138A (2) |
| NO (1) | NO802092L (2) |
| SE (1) | SE8003518L (2) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4483922A (en) * | 1982-05-14 | 1984-11-20 | Amf Inc. | Inactivation of enzymes |
| US4757001A (en) * | 1984-02-16 | 1988-07-12 | Fujirebio Kabushiki Kaisha | Method of measuring biological ligand by utilizing amylase |
| US6218163B1 (en) | 1995-08-07 | 2001-04-17 | Thermogen, Inc. | Stable biocatalysts for ester hydrolysis |
| AU6897496A (en) * | 1996-01-11 | 1997-08-01 | Thermogen, Inc. | Stable biocatalysts for ester hydrolysis |
| JP4220603B2 (ja) * | 1998-12-02 | 2009-02-04 | アルフレッサファーマ株式会社 | 血小板活性化因子アセチルヒドロラーゼ活性の測定方法 |
| CN113533012B (zh) * | 2020-04-22 | 2024-03-12 | 上海云泽生物科技有限公司 | 用于伏立康唑血药浓度检测的样本前处理液 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3119668A (en) * | 1959-01-26 | 1964-01-28 | Dow Chemical Co | Sulfhydryl tests and compounds therefor |
| FR1472956A (2) * | 1964-07-20 | 1967-06-01 | ||
| FR2273283A1 (2) * | 1974-05-28 | 1975-12-26 | Dainippon Pharmaceutical Co |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3867259A (en) * | 1973-11-08 | 1975-02-18 | American Cyanamid Co | Lactate dehydrogenase test material |
| US3875014A (en) * | 1973-12-14 | 1975-04-01 | American Cyanamid Co | Glutamic oxaloacetic transaminase test material |
| US3917515A (en) * | 1974-03-13 | 1975-11-04 | Jack M Goldberg | Serum lipase method and medium |
-
1979
- 1979-07-13 US US06/057,525 patent/US4279994A/en not_active Expired - Lifetime
-
1980
- 1980-05-02 CA CA351,122A patent/CA1128843A/en not_active Expired
- 1980-05-09 SE SE8003518A patent/SE8003518L/xx not_active Application Discontinuation
- 1980-05-20 GB GB8016588A patent/GB2065881B/en not_active Expired
- 1980-05-20 BE BE0/200684A patent/BE883387A/fr not_active IP Right Cessation
- 1980-05-21 DK DK221380A patent/DK221380A/da not_active Application Discontinuation
- 1980-05-27 DE DE19803020072 patent/DE3020072A1/de not_active Withdrawn
- 1980-05-27 JP JP7071980A patent/JPS5615698A/ja active Pending
- 1980-05-29 NL NL8003138A patent/NL8003138A/nl not_active Application Discontinuation
- 1980-06-04 FI FI801799A patent/FI801799A7/fi not_active Application Discontinuation
- 1980-06-26 IT IT8023006A patent/IT1209237B/it active
- 1980-07-07 FR FR8015088A patent/FR2461007A1/fr active Granted
- 1980-07-11 NO NO802092A patent/NO802092L/no unknown
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3119668A (en) * | 1959-01-26 | 1964-01-28 | Dow Chemical Co | Sulfhydryl tests and compounds therefor |
| FR1472956A (2) * | 1964-07-20 | 1967-06-01 | ||
| GB1112251A (en) * | 1964-07-20 | 1968-05-01 | Miles Lab | Diagnostic composition and method |
| FR2273283A1 (2) * | 1974-05-28 | 1975-12-26 | Dainippon Pharmaceutical Co | |
| US3986930A (en) * | 1974-05-28 | 1976-10-19 | Dainippon Pharmaceutical Co., Ltd. | Lipase activity determining method and reagent |
Non-Patent Citations (1)
| Title |
|---|
| EXBK/73 * |
Also Published As
| Publication number | Publication date |
|---|---|
| GB2065881B (en) | 1983-06-22 |
| CA1128843A (en) | 1982-08-03 |
| BE883387A (fr) | 1980-11-20 |
| DK221380A (da) | 1981-01-14 |
| US4279994A (en) | 1981-07-21 |
| NO802092L (no) | 1981-01-14 |
| NL8003138A (nl) | 1981-01-15 |
| DE3020072A1 (de) | 1981-02-05 |
| GB2065881A (en) | 1981-07-01 |
| IT8023006A0 (it) | 1980-06-26 |
| IT1209237B (it) | 1989-07-16 |
| SE8003518L (sv) | 1981-01-14 |
| JPS5615698A (en) | 1981-02-14 |
| FR2461007B1 (2) | 1984-09-14 |
| FI801799A7 (fi) | 1981-01-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Aebi | Catalase | |
| Nishio et al. | Cigarette smoke extract inhibits plasma paraoxonase activity by modification of the enzyme's free thiols | |
| US4543326A (en) | Stabilization of oxidase | |
| CA1139201A (en) | Reagent for the determination of lipase and process for the preparation thereof | |
| NL8500181A (nl) | Voor gebruik gereed vloeibaar reagens ter bepaling van het glucosegehalte in bloed. | |
| US3997470A (en) | Surfactant containing reagent formulations for assaying biological specimens and methods of preparing same | |
| Moss et al. | Kinetic analysis of differences in brain acetylcholinesterase from fish or mammalian sources | |
| FR2461007A1 (fr) | Procede et reactif pour le dosage de la lipase dans les liquides biologiques | |
| EP0155427B1 (fr) | Nouveau milieu de culture, solide, à délitement rapide, prêt à l'emploi et procédé pour le préparer | |
| US3950133A (en) | Reagent formulations for assaying biological specimens and methods of preparing and using same | |
| RO111933B1 (ro) | Derivati de n-feniltiouree, procedeu pentru prepararea acestora si compozitie farmaceutica | |
| JPH0698032B2 (ja) | クレアチンもしくはクレアチニンの測定法及びこれらの測定用試薬 | |
| ES2299704T3 (es) | Procedimiento de cuantificacion de colesterol en lipoproteina de alta densidad y composiciones de reactivos. | |
| JP4018237B2 (ja) | 酵素活性測定用リパーゼ基質溶液およびリパーゼ活性測定用試薬キット | |
| JP2700501B2 (ja) | リパーゼ定量用単一試薬系およびその製造方法 | |
| CH642456A5 (fr) | Composition diagnostique liquide stabilisee contenant un phosphate et procede de preparation de celle-ci. | |
| JPH07155196A (ja) | 生体成分の測定法 | |
| US3953295A (en) | Reagent formulations for glucose assay | |
| Chen et al. | Retinyl esterase activity of purified rat liver retinyl ester lipoprotein complex | |
| JPH01304898A (ja) | 生物試料液の濁りを除去するための試薬 | |
| Salvayre et al. | Fluorometric assay for pancreatic lipase. | |
| JP4013108B2 (ja) | リパーゼの安定化方法 | |
| Hansen et al. | Determination of acetylcholinesterase and pseudocholinesterase in gastrointestinal biopsy tissue | |
| Hochkoeppler et al. | A spectrophotometric lipase activity assay by coupled lipase-lipoxygenase in reverse micelles | |
| FR2661920A1 (fr) | Procede de determination d'un activateur du plasminogene et de son inhibiteur. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ST | Notification of lapse |