FI79120B - Fusionerad gen och foerfarande foer framstaellning av ett terapeutiskt anvaendbart hybridprotein. - Google Patents

Fusionerad gen och foerfarande foer framstaellning av ett terapeutiskt anvaendbart hybridprotein. Download PDF

Info

Publication number
FI79120B
FI79120B FI840090A FI840090A FI79120B FI 79120 B FI79120 B FI 79120B FI 840090 A FI840090 A FI 840090A FI 840090 A FI840090 A FI 840090A FI 79120 B FI79120 B FI 79120B
Authority
FI
Finland
Prior art keywords
fragment
encoding
gene
hybrid protein
region
Prior art date
Application number
FI840090A
Other languages
English (en)
Finnish (fi)
Other versions
FI840090A0 (fi
FI79120C (sv
FI840090A (fi
Inventor
John R Murphy
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Publication of FI840090A0 publication Critical patent/FI840090A0/fi
Publication of FI840090A publication Critical patent/FI840090A/fi
Application granted granted Critical
Publication of FI79120B publication Critical patent/FI79120B/fi
Publication of FI79120C publication Critical patent/FI79120C/sv

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/665—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans derived from pro-opiomelanocortin, pro-enkephalin or pro-dynorphin
    • C07K14/68—Melanocyte-stimulating hormone [MSH]
    • C07K14/685—Alpha-melanotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/665—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans derived from pro-opiomelanocortin, pro-enkephalin or pro-dynorphin
    • C07K14/68—Melanocyte-stimulating hormone [MSH]
    • C07K14/69—Beta-melanotropin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/31—Linker sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Amplifiers (AREA)
  • Secondary Cells (AREA)
  • Adhesives Or Adhesive Processes (AREA)

Claims (14)

1. Fusionerad gen, kännetecknad därav, att den omfattar ett genfragment, som kodar difteriatoxin, 5 fusionerat med ett genfragment som kodar för en cellspeci-fik polypeptidligand, varvid den fusionerade genen kodar för ett hybridprotein och varvid difteriatoxinfragmentet inkluderar en region som kodar för det hyrdofoba ledar-fragmentet i difteriatoxinet, en region som kodar för det 10 entzymatiskt aktiva fragmentet A i difteriatoxinet, en region som kodar för den proteaskänsliga slingan invid fragmentet A av difteriatoxinet, och ätminstone en del av regionen som kodar för den hydrofoba regionen i fragmentet B, ändä utan den region som kodar för det allmänna euka-15 ryotiska bindningsstället i fragmentet B, varvid nämnda fragment, som kodar för polypeptidliganden, kodar för en sädan del av liganden som förorsakar att hybridproteinet binder sig selektivt vid en i förväg bestämd klass av cel-ler.
2. Fusionerad gen enligt patentkravet 1, kän netecknad därav, att regionen som kodar för den hydrofoba regionen i fragmentet B omfattar en region som kodar för 12 .
3. Fusionerad gen enligt patentkravet 1 eller 2, 25 kännetecknad därav, att polypeptidliganden är ett a- eller β-melanocytstimulerande hormon.
4. Kloningsvektor, kännetecknad därav, att den innehäller en fusionerad gen enligt patentkravet 1, 2 eller 3.
5. Användning av en kloningsvektor enligt patent kravet 4 för expression av aminosyrasekvens genom ett sed-vanligt förfarande. 2i 79120
6. Förfarande för framställning av ett terapeu-tiskt användbart hybridprotein bestäende av proteinfrag-ment, vilka förenats genora peptidbingningar, varvid hyb-ridproteinet i följd med början vid den aminoterminala 5 änden av hybridproteinet omfattar a) det enzymatiskt aktiva fragmentet A av difteriatoxin, b) ett fragment som inkluderar spjälkningsomrädet lx invid fragmentet A av difteriatoxin, 10 c) ett fragment som omfattar ätminstone en del av den hydrofoba regionen i fragmentet N i difteriatoxin, och inte innehäller det allmänna eukaryotriska bindningsställ-et i nämnda fragment B, och d) ett fragment som omfattar en del av en cellspe-15 cifik polypeptidligand, varvid denna del inkluderar ät-minstone en del av bindningsomrädet av polypeptidliganden, varvid denna del av bindningsomrädet förmär förorsaka att hybridproteinet binder sig selektivt vid en i förväg be-stämd klass av celler, vilka päverkas av det enzymatiskt 20 aktiva fragmentet A, kännetecknat därav, att en fusionerad gen enligt patentkravet 1, vilken kodar för a), b), c) och d), inkorporeras med en kloningsvektor, en värdcell transformers med denna kloningsvektor och denna kloningsvektor 25 och värdcellen odlas för produktion av hybridproteinet.
7. Förfarande enligt patentkravet 6, kännetecknat därav, att fragmentet c) kodas av den del av genfragmentet som kodar för fragmentet B, vilken ligger mellan lx och positionen för NRU I-stället. 30
8. Förfarande enligt patentkravet 6 eller 7, kännetecknat därav, att hybridproteinet yt-terligare omfattar fragmentet 12 mellan fragmenten c) och d).
FI840090A 1982-05-12 1984-01-11 Fusionerad gen och förfarande för framställning av ett terapeutiskt an vändbart hybridprotein FI79120C (sv)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US37738682A 1982-05-12 1982-05-12
US37738682 1982-05-12
PCT/US1983/000723 WO1983003971A1 (en) 1982-05-12 1983-05-12 Hybrid proteins
US8300723 1983-05-12

Publications (4)

Publication Number Publication Date
FI840090A0 FI840090A0 (fi) 1984-01-11
FI840090A FI840090A (fi) 1984-01-11
FI79120B true FI79120B (fi) 1989-07-31
FI79120C FI79120C (sv) 1989-11-10

Family

ID=23488913

Family Applications (1)

Application Number Title Priority Date Filing Date
FI840090A FI79120C (sv) 1982-05-12 1984-01-11 Fusionerad gen och förfarande för framställning av ett terapeutiskt an vändbart hybridprotein

Country Status (12)

Country Link
US (1) US4675382A (sv)
EP (1) EP0108146B1 (sv)
JP (3) JP2534222B2 (sv)
AT (1) ATE25197T1 (sv)
AU (1) AU573529B2 (sv)
DE (1) DE3369466D1 (sv)
ES (1) ES8407097A1 (sv)
FI (1) FI79120C (sv)
IT (1) IT1203672B (sv)
NO (1) NO176575C (sv)
NZ (1) NZ204229A (sv)
WO (1) WO1983003971A1 (sv)

Families Citing this family (115)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5080898A (en) * 1982-05-12 1992-01-14 The University Hospital Enzymatically active toxin coupled to a cell-specific ligand
FR2549855B1 (fr) * 1983-07-29 1987-03-27 Grp Genie Genetique Sequence nucleotidique codant pour le peptide signal de la toxine diphterique, vecteur contenant cette sequence nucleotidique, leur application a la transformation de micro-organismes et compositions peptidiques obtenues
DE3346953A1 (de) * 1983-12-24 1985-08-14 Organogen Medizinisch-Molekularbiologische Forschungsgesellschaft mbH, 6900 Heidelberg Cardiodilatin, ein neues peptidhormon und verfahren zu seiner herstellung
DE3584486D1 (de) * 1984-02-08 1991-11-28 Cetus Corp Toxinkonjugate.
US6022950A (en) * 1984-06-07 2000-02-08 Seragen, Inc. Hybrid molecules having translocation region and cell-binding region
US5668255A (en) * 1984-06-07 1997-09-16 Seragen, Inc. Hybrid molecules having translocation region and cell-binding region
ES8708014A1 (es) * 1984-06-07 1987-09-01 Murphy John R Un procedimiento para preparar un gen fusionado que codifica una proteina hibrida susceptible de marcado y empleo en diagnosis.
US5807715A (en) * 1984-08-27 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods and transformed mammalian lymphocyte cells for producing functional antigen-binding protein including chimeric immunoglobulin
GB8422238D0 (en) * 1984-09-03 1984-10-10 Neuberger M S Chimeric proteins
US6103243A (en) * 1985-05-15 2000-08-15 Biotechnology Australia Pty, Ltd Oral vaccines
IL78775A (en) * 1985-05-15 1992-06-21 Biotech Australia Pty Ltd Oral vaccines
US5169939A (en) * 1985-05-21 1992-12-08 Massachusetts Institute Of Technology & Pres. & Fellows Of Harvard College Chimeric antibodies
US5011684A (en) * 1985-09-05 1991-04-30 Beth Israel Hospital Association Lysing or blocking unwanted cells with IL-2 receptor-specific binding substance
US5336489A (en) * 1985-09-05 1994-08-09 The Beth Israel Hospital Association Treatment of allograft rejection with IL-2 receptor-specific cytotoxins
US5576195A (en) * 1985-11-01 1996-11-19 Xoma Corporation Vectors with pectate lyase signal sequence
US5618920A (en) * 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
WO1987002987A1 (en) * 1985-11-13 1987-05-21 Murphy John R Cys codon-modified dna
US4962188A (en) * 1985-12-06 1990-10-09 Cetus Corporation Recombinant ricin toxin A chain conjugates
US6051405A (en) * 1986-09-24 2000-04-18 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Constructs encoding recombinant antibody-toxin fusion proteins
US4892827A (en) * 1986-09-24 1990-01-09 The United States Of America As Represented By The Department Of Health And Human Services Recombinant pseudomonas exotoxins: construction of an active immunotoxin with low side effects
US5082927A (en) * 1986-09-24 1992-01-21 The United States Of America As Represented By The Department Of Health And Human Services Selectively cytotoxic IL-4-PE40 fusion protein
IL84285A (en) * 1986-10-27 1993-03-15 Int Genetic Engineering Chimeric antibody with specificity to human tumor antigen
US6893625B1 (en) 1986-10-27 2005-05-17 Royalty Pharma Finance Trust Chimeric antibody with specificity to human B cell surface antigen
IL85035A0 (en) * 1987-01-08 1988-06-30 Int Genetic Eng Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same
US5149788A (en) * 1987-05-19 1992-09-22 Hoffmann-La Roche, Inc. Purification of chimeric proteins containing an IL-2 moiety by receptor-affinity chromatography
DE3856180T2 (de) * 1987-08-20 1998-09-03 Childrens Medical Center Nukleinsaürefragmente, die mannose bindende fragmente vom menschlichen mannosebindenden protein kodieren
US5270199A (en) * 1987-08-20 1993-12-14 The Children's Medical Center Corporation Human mannose-binding protein
US5792458A (en) * 1987-10-05 1998-08-11 The United States Of America As Represented By The Department Of Health And Human Services Mutant diphtheria toxin conjugates
US5084556A (en) * 1987-10-23 1992-01-28 Genetics Institute, Inc. Composition of M-CSF conjugated to cytotoxic agents and a method for treating cancers characterized by over-expression of the c-fms proto-oncogene
JP2518911B2 (ja) * 1987-10-23 1996-07-31 森永乳業株式会社 ｃ―ｆｍｓ癌原遺伝子の過剰発現によって特徴づけられた癌の処置用組成物および方法
US6004781A (en) * 1988-01-22 1999-12-21 The General Hospital Corporation Nucleic acid encoding Ig-CD4 fusion proteins
IL106992A (en) * 1988-02-11 1994-06-24 Bristol Myers Squibb Co Noble hydrazonic history of anthracycline and methods for their preparation
US5827934A (en) * 1988-03-08 1998-10-27 The University Of Wyoming Cytotoxic diphtheria toxin fragments
US5582862A (en) 1988-04-04 1996-12-10 General Hospital Corporation Antibodies that bind to α2-antiplasmin crosslinked to fibrin which do not inhibit plasma α2-antiplasmin
US5372812A (en) * 1988-04-04 1994-12-13 The General Hospital Corporation Composition and method for acceleration of clot lysis
US5290914A (en) * 1988-04-28 1994-03-01 Mycogen Corporation Hybrid diphtheria-B.t. pesticidal toxins
US5152980A (en) * 1988-05-19 1992-10-06 The Beth Israel Hospital Association Induction of tolerance to a foreign antigen IL-2 receptor-binding substances
JPH03504248A (ja) * 1988-05-19 1991-09-19 ザ・ベス・イスラエル・ホスピタル・アソシエーション 外来抗原に対する寛容の誘発
US6106840A (en) * 1988-06-23 2000-08-22 Anergen, Inc. MHC conjugates useful in ameliorating autoimmunity
US5194425A (en) * 1988-06-23 1993-03-16 Anergen, Inc. Mhc-mediated toxic conjugates useful in ameliorating autoimmunity
US5260422A (en) * 1988-06-23 1993-11-09 Anergen, Inc. MHC conjugates useful in ameliorating autoimmunity
US5468481A (en) * 1988-06-23 1995-11-21 Amergen, Inc. MHC class II-peptide conjugates useful in ameliorating autoimmunity
JPH03500415A (ja) * 1988-07-05 1991-01-31 アムジエン・インコーポレーテツド インターロイキン２類似体
US5135736A (en) * 1988-08-15 1992-08-04 Neorx Corporation Covalently-linked complexes and methods for enhanced cytotoxicity and imaging
US5169933A (en) * 1988-08-15 1992-12-08 Neorx Corporation Covalently-linked complexes and methods for enhanced cytotoxicity and imaging
US5609869A (en) * 1988-08-19 1997-03-11 The General Hospital Corporation Hybrid immunoglobulin-thrombolytic enzyme molecules which specifically bind a thrombus, and methods of their production and use
US5811265A (en) * 1988-08-19 1998-09-22 The General Hospital Corporation Hybrid immunoglobulin-thrombolytic enzyme molecules which specifically bind a thrombus, and methods of their production and use
US5632991A (en) * 1988-11-14 1997-05-27 Brigham & Women's Hospital Antibodies specific for E-selectin and the uses thereof
JPH02169521A (ja) * 1988-12-22 1990-06-29 Ajinomoto Co Inc 自己免疫疾患治療剤
DE4004573A1 (de) * 1989-02-17 1990-08-23 Tanabe Seiyaku Co Neues versuchstier und seine herstellung
US5621078A (en) * 1989-03-22 1997-04-15 Merck & Co., Inc. Modified pseudomonas exotoxin PE40
AU5355790A (en) * 1989-04-19 1990-11-16 Cetus Corporation Multifunctional m-csf proteins and genes encoding therefor
ES2091824T3 (es) * 1989-04-21 1996-11-16 Us Health Proteina recombinante de fusion toxina anticuerpo.
IE901736L (en) * 1989-05-17 1990-11-17 Fuller H B Licensing Financ Polypeptide-antibody conjugate for inhibiting cell adhesion
WO1991001146A1 (en) * 1989-07-14 1991-02-07 Praxis Biologics, Inc. Cytokine and hormone carriers for conjugate vaccines
US6207798B1 (en) 1989-08-03 2001-03-27 Merck & Co., Inc. Modified PE40 toxin fusion proteins
KR927003613A (ko) * 1989-08-23 1992-12-18 더 제너랄 호스피탈 코오퍼레이숀 비-인체 영장동물의 cd4폴리텝타이드, 글리코실화할 수 있는 인체 cd4분자, 그것의 단편, 그것의 융합 단백질, 유전자 서열 및 그것의 용도
JPH05502880A (ja) * 1989-12-22 1993-05-20 セラジェン・インコーポレーテッド トランスロケーション領域および細胞結合領域を有するハイブリッド分子
US5340935A (en) * 1990-01-05 1994-08-23 Dana-Farber Cancer Institute, Inc. DNAS encoding proteins active in lymphocyte-medicated cytotoxicity
DK0517829T4 (da) * 1990-03-02 2008-03-03 Boston Medical Ct Corp Forbedrede kimæriske toksiner
US5110912A (en) * 1990-03-02 1992-05-05 Seragen, Inc. Purification of il-2-containing hybrid compounds
US5137877B1 (en) * 1990-05-14 1996-01-30 Bristol Myers Squibb Co Bifunctional linking compounds conjugates and methods for their production
CA2083487A1 (en) * 1990-06-13 1991-12-14 John R. Murphy Chimeric toxins with improved inter-domain geometry
IL98528A0 (en) * 1990-06-21 1992-07-15 Merck & Co Inc Pharmaceutical compositions containing hybrid for killing bladder cancer cells
NO175188C (no) * 1990-06-27 1994-09-14 Sjur Olsnes Fremgangsmåte for fremstilling av et peptidkonjugat med evne til å trenge inn i cellecytosol
US5238823A (en) * 1990-08-22 1993-08-24 Veterinary Infectious Disease Organization Interleukin-2-leukotoxin gene fusions and uses thereof
US5273889A (en) * 1990-08-22 1993-12-28 University Of Saskatchewan Gamma-iterferon-leukotoxin gene fusions and uses thereof
US5594107A (en) * 1990-08-22 1997-01-14 University Of Saskatchewan Chimeric protein comprising an RTX-family cytotoxin and interferon-2 or interferon
AU651152B2 (en) * 1990-08-29 1994-07-14 Genetics Institute, Llc Multidomain hematopoiesis stimulators
WO1992006117A1 (en) * 1990-09-28 1992-04-16 Seragen, Inc. Inhibiting unwanted immune responses
CA2069746A1 (en) * 1990-09-28 1992-03-29 Jonathan I. Rosen Hybrid growth factors
US5328984A (en) * 1991-03-04 1994-07-12 The United States As Represented By The Department Of Health & Human Services Recombinant chimeric proteins deliverable across cellular membranes into cytosol of target cells
WO1992019259A1 (en) * 1991-05-03 1992-11-12 Seragen, Inc. Interleukin receptor targeted molecules for treatment of inflammatory arthritis
JPH06509563A (ja) * 1991-07-05 1994-10-27 セラゲン・インコーポレイテッド 炎症性関節炎の治療用の表皮細胞成長因子受容体を標的とする分子
US6146850A (en) * 1991-11-04 2000-11-14 Xoma Corporation Proteins encoding gelonin sequences
US5621083A (en) 1991-11-04 1997-04-15 Xoma Corporation Immunotoxins comprising ribosome-inactivating proteins
US5837491A (en) * 1991-11-04 1998-11-17 Xoma Corporation Polynucleotides encoding gelonin sequences
US5571507A (en) * 1992-02-25 1996-11-05 Seragen, Inc. Methods of treating diabetes
GB9219562D0 (en) 1992-03-11 1992-10-28 Prendergast Kennet F Anti-viral peptides
CA2118010C (en) * 1992-04-13 2003-10-28 Donald Kufe Antibodies specific for carcinoma-associated antigens
DK96493D0 (da) * 1993-08-26 1993-08-26 Mouritsen Og Elsner Aps Fremgangsmaade til at inducere antistofresponser mod selvproteiner og autovaccine fremstillet ved fremgangsmaaden
US5804452A (en) * 1995-04-27 1998-09-08 Quidel Corporation One step urine creatinine assays
WO1997013410A1 (en) * 1995-10-13 1997-04-17 Boston Medical Center Corporation Hybrid molecules containing amidated polypeptide binding ligands
WO1997023243A1 (en) * 1995-12-22 1997-07-03 Bristol-Myers Squibb Company Branched hydrazone linkers
AU2610097A (en) * 1996-04-10 1997-10-29 Sangstat Medical Corporation Cytomodulating conjugates of members of specific binding pairs
JP2001502895A (ja) * 1996-09-20 2001-03-06 ザ・ジェネラル・ホスピタル・コーポレイション アルファ―２―抗プラスミンに対するキメラ、ヒト化および一本鎖抗体
AU6587998A (en) * 1997-03-27 1998-10-20 Demeter Biotechnologies, Ltd. Ligand/lytic peptide compositions and methods of use
US6635740B1 (en) 1997-03-27 2003-10-21 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Ligand/lytic peptide compositions and methods of use
US7741435B2 (en) * 1997-07-09 2010-06-22 Advanced Targeting Systems, Inc. Substance P-saporin (SP-SAP) conjugates and methods of use thereof
US6251866B1 (en) 1997-08-05 2001-06-26 Watson Laboratories, Inc. Conjugates targeted to the interleukin-2 receptor
US6680058B1 (en) 1997-09-03 2004-01-20 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Compositions and methods for contraception in or sterilization of mammals
US6399575B1 (en) * 1998-11-10 2002-06-04 Auburn University Methods and compositions for targeting compounds to the central nervous system
CA2392456A1 (en) 1999-11-24 2001-05-31 Oklahoma Medical Research Foundation Assays and therapies for latent viral infection
JP5059271B2 (ja) * 2000-03-31 2012-10-24 パーデュー・リサーチ・ファウンデイション リガンド免疫原複合体を用いる処置方法
AU2001275930B2 (en) * 2000-07-14 2007-05-10 Eisai Inc. Alpha-msh related compounds and methods of use
ATE447965T1 (de) * 2000-09-11 2009-11-15 Dana Farber Cancer Inst Inc Muc1 extrazelluläre domäne und zusammensetzungen für krebsbehandlung, und davon abgeleitete verfahren
EP1958642A1 (en) * 2000-12-22 2008-08-20 Dana-Farber Cancer Institute Regulation of cell growth by MUC1
WO2002082081A2 (en) * 2001-04-06 2002-10-17 University Of Rochester Suppression of androgen receptor transactivation through new pathways to ar and ar coactivators and repressors
GB0112687D0 (en) * 2001-05-24 2001-07-18 Microbiological Res Authority Pharmaceutical use of secreted bacterial effector proteins
US20040002058A1 (en) * 2001-06-21 2004-01-01 Uab Research Foundation Chimeric capsid proteins and uses thereof
US20030124144A1 (en) * 2001-06-21 2003-07-03 Larry Cosenza Chimeric capsid proteins and uses thereof
US20030124742A1 (en) * 2001-08-22 2003-07-03 Prakash Ramesh K. Conjugates targeted to target receptors
EP1434603B1 (en) * 2001-09-28 2009-12-16 Purdue Research Foundation Method of treatment using ligand-immunogen conjugates
US6846484B2 (en) 2001-12-28 2005-01-25 Regents Of The University Of Minnesota DTAT fusion toxin
WO2004092339A2 (en) * 2003-04-11 2004-10-28 Ilex Products, Inc. Modulation of muc1 mediated signal transduction
US20060257428A1 (en) * 2003-08-22 2006-11-16 Harley John B Assays and therapies for latent viral infection
WO2005042573A1 (en) 2003-10-24 2005-05-12 Dana-Farber Cancer Institute, Inc. Modulation of the interaction of muc1 with muc1 ligands
EP1687016A4 (en) 2003-11-25 2009-07-29 Anjin Corp GENETIC VARIANT OF DIPHTHERIC TOXIN
JP2007523214A (ja) * 2004-02-23 2007-08-16 ジェンザイム コーポレイション デスレセプターリガンド誘導アポトーシスのｍｕｃ１アンタゴニスト増強方法
US20110104186A1 (en) 2004-06-24 2011-05-05 Nicholas Valiante Small molecule immunopotentiators and assays for their detection
SI3207941T1 (sl) 2006-09-07 2020-08-31 Scott & White Memorial Hospital Postopki in sestavki na osnovi konjugatov toksina davice-interlevkin-3
US7871784B2 (en) * 2007-02-02 2011-01-18 Dana-Farber Cancer Institute, Inc. Methods and compositions relating to the regulation of apoptosis by MUC1 and BH3-containing proapoptotic proteins
US7972870B2 (en) 2007-02-02 2011-07-05 Dana-Farber Cancer Institute, Inc. Methods and compositions relating to the regulation of MUC1 by HSF1 and STAT3
EP2346904B1 (en) 2008-10-29 2017-04-12 China Synthetic Rubber Corporation Methods and agents for the diagnosis and treatment of hepatocellular carcinoma
WO2012068463A2 (en) 2010-11-18 2012-05-24 Beth Israel Deaconess Medicall Center, Inc. Methods of treating obesity by inhibiting nicotinamide n-methyl transferase (nnmt)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4201770A (en) * 1973-05-07 1980-05-06 The Ohio State University Antigenic modification of polypeptides
US4302386A (en) * 1978-08-25 1981-11-24 The Ohio State University Antigenic modification of polypeptides
US4082613A (en) * 1976-04-23 1978-04-04 The Regents Of The University Of Minnesota Process for the production of insulin by genetically transformed fungal cells
JPS5470384A (en) * 1977-08-22 1979-06-06 Inst Obu Kiyansaa Risaachi Roi High molecular complex
US4336336A (en) * 1979-01-12 1982-06-22 President And Fellows Of Harvard College Fused gene and method of making and using same
JPS5616418A (en) * 1979-07-20 1981-02-17 Teijin Ltd Antitumor protein complex and its preparation
JPS5686121A (en) * 1979-12-14 1981-07-13 Teijin Ltd Antitumor proten complex and its preparation
FR2493846A1 (fr) * 1980-11-10 1982-05-14 Delbarre B Derives du 4h-benzo(4,5)cyclohepta(1,2-b)furanne, leur procede de preparation et leur application en tant que medicaments
US4394443A (en) * 1980-12-18 1983-07-19 Yale University Method for cloning genes
FR2504010B1 (fr) * 1981-04-15 1985-10-25 Sanofi Sa Medicaments anticancereux contenant la chaine a de la ricine associee a un anticorps antimelanome et procede pour leur preparation

Also Published As

Publication number Publication date
JPS59500814A (ja) 1984-05-10
NO840089L (no) 1984-01-11
IT1203672B (it) 1989-02-15
ES522315A0 (es) 1984-08-16
US4675382A (en) 1987-06-23
FI840090A0 (fi) 1984-01-11
FI79120C (sv) 1989-11-10
EP0108146A4 (en) 1984-05-03
NZ204229A (en) 1986-01-24
WO1983003971A1 (en) 1983-11-24
EP0108146A1 (en) 1984-05-16
NO176575C (no) 1995-04-26
EP0108146B1 (en) 1987-01-28
ATE25197T1 (de) 1987-02-15
NO176575B (no) 1995-01-16
AU1706283A (en) 1983-12-02
JPH08228777A (ja) 1996-09-10
JPH06239896A (ja) 1994-08-30
ES8407097A1 (es) 1984-08-16
IT8367525A0 (it) 1983-05-12
AU573529B2 (en) 1988-06-16
FI840090A (fi) 1984-01-11
DE3369466D1 (en) 1987-03-05
JP2534222B2 (ja) 1996-09-11

Similar Documents

Publication Publication Date Title
FI79120B (fi) Fusionerad gen och foerfarande foer framstaellning av ett terapeutiskt anvaendbart hybridprotein.
US5080898A (en) Enzymatically active toxin coupled to a cell-specific ligand
JP2534221B2 (ja) 牛のプレ成長および成長ホルモン
US5889149A (en) Purified PDGF AB isoform and method of making it
FI100253B (sv) Hybridprotein och förfarande för framställning och användning därav
US5624827A (en) DNA sequences encoding the plant toxin gelonin
NL8900178A (nl) Groei regulerende clycoproteinen.
JPH02501112A (ja) 免疫親和性による精製方法
EP0246307B1 (en) Cys codon-modified dna
JPS61219395A (ja) ＴＧＦ‐βをコードしている核酸およびその用途
WO1991007418A1 (en) Chimeric mouse-human a10 antibody with specificity to a human tumor cell antigen
JPH05500310A (ja) 融合蛋白質の製造方法
WO1985002198A1 (en) Microbial expression of type i transforming growth factor, polypeptide analogs thereof and hybrid egf/tgf polypeptides
DE3853842T2 (de) Wachstumsregler zusammenhängend mit Blutplättchen.
JP2771220B2 (ja) 植物性リボソーム不活化タンパク質をコードするヌクレオチド配列
EP0510018A1 (en) Lipoprotein signal peptide fused to antigenic polypeptides
US5411732A (en) Preparation of fused proteins, antibodies and processes therefore
CA1217156A (en) Hybrid proteins
AU611048B2 (en) Genetic expression of somatostatin as hybrid polypeptide
US5166316A (en) Physiologically active peptides and a method of producing peptides
JPH05509320A (ja) 組織誘導型腫瘍成長抑制物質、その調製及び使用方法
JPS60246322A (ja) ヒトインタ−ロイキン−２に対する抗体
JPS63245691A (ja) ヒト免疫グロブリンＧＦｃ領域蛋白質およびその製造方法
JPS61501168A (ja) 生長因子における改良
NZ227372A (en) Murine monoclonal antibodies to human ifnalpha

Legal Events

Date Code Title Description
MA Patent expired

Owner name: PRESIDENT AND FELLOWS OF HARVARD COLLEGE