FI120045B - Behandling av cellulosamaterial och däri användbara enzym - Google Patents

Behandling av cellulosamaterial och däri användbara enzym Download PDF

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Publication number
FI120045B
FI120045B FI20051318A FI20051318A FI120045B FI 120045 B FI120045 B FI 120045B FI 20051318 A FI20051318 A FI 20051318A FI 20051318 A FI20051318 A FI 20051318A FI 120045 B FI120045 B FI 120045B
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FI
Finland
Prior art keywords
dsm
acremonium
thermophilum
enzyme
enzymes
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FI20051318A
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English (en)
Finnish (fi)
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FI20051318A (sv
FI20051318A0 (sv
Inventor
Liisa Viikari
Matti Siika-Aho
Jari Vehmaanperae
Jarno Kallio
Marika Alapuranen
Terhi Puranen
Satu Hooman
Sanni Voutilainen
Teemu Halonen
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Roal Oy
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38188306&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=FI120045(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Roal Oy filed Critical Roal Oy
Priority to FI20051318A priority Critical patent/FI120045B/sv
Publication of FI20051318A0 publication Critical patent/FI20051318A0/sv
Priority to AP2008004509A priority patent/AP2924A/xx
Priority to CA2833029A priority patent/CA2833029C/en
Priority to DK12151852.6T priority patent/DK2453014T3/en
Priority to BRPI0620287A priority patent/BRPI0620287B1/pt
Priority to CN201110021502.9A priority patent/CN102174492B/zh
Priority to AU2006326963A priority patent/AU2006326963B2/en
Priority to RU2008123954/10A priority patent/RU2458128C2/ru
Priority to PCT/FI2006/050558 priority patent/WO2007071818A1/en
Priority to CA2632502A priority patent/CA2632502C/en
Priority to CN2006800528641A priority patent/CN101384713B/zh
Priority to ES06830936.8T priority patent/ES2501944T3/es
Priority to EP06830936.8A priority patent/EP1963498B1/en
Priority to ES10191152.7T priority patent/ES2527681T3/es
Priority to EP10191152.7A priority patent/EP2319920B1/en
Priority to EP12151850.0A priority patent/EP2453013B1/en
Priority to EP12151852.6A priority patent/EP2453014B1/en
Priority to DK12151850.0T priority patent/DK2453013T3/en
Priority to ES12151850.0T priority patent/ES2582320T3/es
Priority to ES12151852.6T priority patent/ES2582078T3/es
Priority to ARP060105762A priority patent/AR058721A1/es
Priority to TW095148542A priority patent/TWI391489B/zh
Publication of FI20051318A publication Critical patent/FI20051318A/sv
Priority to ZA200804976A priority patent/ZA200804976B/xx
Priority to US12/141,976 priority patent/US20090042266A1/en
Publication of FI120045B publication Critical patent/FI120045B/sv
Application granted granted Critical
Priority to US12/917,603 priority patent/US8409836B2/en
Priority to US13/774,465 priority patent/US9758777B2/en
Priority to US14/045,236 priority patent/US9593324B2/en
Priority to US15/690,513 priority patent/US20180044656A1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445Beta-glucosidase (3.2.1.21)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides
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    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/08Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021Beta-glucosidase (3.2.1.21)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (24)

1. Förfarande för att behandfa cellulosamaterial med celiobiohydro-las, endogiukanas och hetaglukosidas, kännetecknat av att ceflobiohyd- 5 roiaset innehälier en aminosyrasekvens med minst 80 % identitet med sekvens nr 28.
2. Förfarande enligt patentkrav 1, kännete c k n at av att endo-giukanaset innehälier en aminosyrasekvens med minst 80 % identitet med sekvens nr 10, 12, 14 elier 16, elier med ett enzymatiskt aktivt fragment därav. to
3. Förfarande enligt patentkrav 2, kännetecknat avattendo- glukanaset kan erhalias ur Thermoascus aurantiacus, Acremonium thermophi-lum, elier Chaetomium thermophilum.
4. Förfarande enligt: patentkrav 3, k ä n n e t e c k n at av att endo-glukanaset kan erhalias ur Thermoascus aurantiacus GBS 116239, Acre moni- 15 um thermophilum GBS 116240, elier Chaetomium thermophilum CBS 730.95.
5. Förfarande enligt patentkrav 1, kännetecknat av att beta-glukpsidaset innehälier en aminosyrasekvens med minst 80 % identitet med sekvens nr 22. 24 elier 26, elier med ett enzymatiskt aktivt fragment därav,
6. Förfarande enligt patentkrav 5, kännetecknat av att beta- 20 glukosidaset kan erhalias ur Thermoascus aurantiacus, Acremonium thermophilum, elier Chaetomium thermophilum, företrädesvis ur Thermoascus aurantiacus CBS 116239, Acremonium thermophilum CBS 116240, elier Chaetomium thermophilum CBS 730.95.
7. Förfarande enligt nägot av de föregäende patentkraven, kan- 25 netecknat av att eeliulosamaterialet ar lignocellulosamaterial.
8. Förfarande enligt nägot av de föregäende patentkraven, ka nne t e c k n a t av att man behandlar lignoceljufosamaterialet med minst ett yt-terligare enzym, företrädesvis xylanas, vilket företrädesvis innehälier en aminosyrasekvens med minst 80 % identet med sekvens nr 18 elier 20, elier med go ett enzymatiskt aktivt fragment därav.
9. Förfarande enligt patentkrav 8, kannet e c kn at av att xyfa-naset kan erhalias ur Thermoascus aurantiacus elier Acremonium thermophilum, företrädesvis ur Thermoascus aurantiacus CBS 116239, eiler Acremonium thermophilum CBS 116240.
10. Förfarande enligt nägot av de föregaende patentkraven, k a n ne tee k na t ay ai! enzymen tillförs i ceiluiosamateriaJet antingen samtidigt eller efter varandra.
11, Förfarande enligt nägot av de föregaende patentkraven, k ä n -5 n e t e c k n a t av att ätminstone ett av enzymen kodas av en gen som är lika- dan som den som inkorporerats i mikroorganismen med deponeringsnummer DSM 17326, DSM 17324, DSM17323, DSM 17729, DSM 16724, DSM 16726, DSM 16726, DSM 17325 eller DSM 17667.
12, Förfarande enligt patentkrav 1, k ä n n e t e c k n a t av att cellu-10 losamaferialet väljs ur gruppen bestaende av majsskörderester, prydnadshirs (switchgrass), balm av säd, sockerrörsbagass och träbaserade material.
13. Enzympreparat, som innehäller cellobiöhydrolas, endoglukanas och betagiukosidas, kännetecknat av att eellobiohydrolaset innehäller an aminosyrasekvens med nriinst 80 % identitet med sekvens nr 28.
14. Enzympreparat enligt patentkrav 13, kanne tee k n a t av att endogiukanaset innehäller en aminosyrasekvens med minst 80 % identitet med sekvens nr 10, 12, 14 eller 16, eller med ett enzymatiskt aktivt fragment därav.
15. Enzympreparat enligt patentkrav 14, k ä n n e t e c k n a t av att 20 endogiukanaset kan erhallas ur Thermoascus aurantiams, Acremonium ther- mophilum, eller Chaetomium thermophilum, företrädesvis ur Thermoascus au-rantiacus CBS 116239, Acremonium thermophilum CBS 116240, eller Chaetomium thermophilum CBS 730.95.
16. Enzympreparat enligt patentkrav 13, k a n n eteek n a t av att 25 betaglukösidaset innehäller en aminosyrasekvens med minst 80 % identitet med sekvens nr 22, 24 eller 26, eller med ett enzymatiskt aktivt fragment därav.
17. Enzympreparat enligt patentkrav 16, k ä n n e t e c k n a t av att betaglukösidaset kan erhallas ur Thermoascus aurantiacus, Acremonium 30 thermophilum, eller Chaetomium thermophilum, företrädesvis ur Thermoascus I aurantiacus CBS 116239, Acremonium thermophilum CBS 116240, eller Chaetomium thermophilum CBS 730,95.
18. Enzympreparat enligt nägot av patentkraven 13-17, k ä n n e -teckn at av att det innehäller ätminstone ett ytterligare enzym, företrädesvis 35 xylanas, vilket företrädesvis innehäller en aminosyrasekvens med minst 80 % identitet med sekvens or 18 eiler 20, eller med ett enzymatiskt aktivt fragment därav.
19. Enzympreparat enligt patentkrav 18, k ä n n e t e e k n a t av att xylanaset kan erhallas ur Thermoascus aurantiacus eller Acremonium ther- 5 mophiium, företrädesvis ur Thermoascus aurantiacus CBS 116239, eller Ac~ remonium thermophilum CBS 116240-
20. Enzympreparat enligt nagot av patentkraven 13 -19, kanne-t e c k n a t av att atminstone ei av enzymen kodas av en gen som är likadan sorrv den som inkorporerats i mikroörganismen med deponeringsnummer DSM 10 17326, DSM 17324, DSM 17323, DSM 17729, DSM 16724, DSM 16726, DSM 16725, DSM 17325 eller DSM 17667.
21. Enzympreparat enligt patentkrav 13, k ä n n e t e ck n a t av att det föreligger i form av använt odlingsmedium, pulver, granulat eller vätska.
22. Anvandnihg av ett enzympreparat enligt nagot av patentkraven 15 13 - 21 för att bryla ner ceiluiosamaterial.
23. Användning enligt patentkrav 22, känneteck n a t av aft cel-lulosamaterialet väljs ur gruppen bestäende av majsskörderester, prydnadshirs (swiichgrass), halm av säd, sockerrörsbagass och träbaserade material.
24. Användning av förfarandet enligt nagot av patentkraven 1 -12 i 20 en process för framställning av etanol ur ceiluiosamaterial.
FI20051318A 2005-12-22 2005-12-22 Behandling av cellulosamaterial och däri användbara enzym FI120045B (sv)

Priority Applications (28)

Application Number Priority Date Filing Date Title
FI20051318A FI120045B (sv) 2005-12-22 2005-12-22 Behandling av cellulosamaterial och däri användbara enzym
ES12151852.6T ES2582078T3 (es) 2005-12-22 2006-12-15 Tratamiento de material celulósico y enzimas útiles en el mismo
ES10191152.7T ES2527681T3 (es) 2005-12-22 2006-12-15 Tratamiento de material celulósico y enzimas útiles en el mismo
EP12151852.6A EP2453014B1 (en) 2005-12-22 2006-12-15 Treatment of cellulosic material and enzymes useful therein
DK12151852.6T DK2453014T3 (en) 2005-12-22 2006-12-15 Treatment of cellulose materials and enzymes useful therefor
BRPI0620287A BRPI0620287B1 (pt) 2005-12-22 2006-12-15 método para tratar material celulósico com celobioidrolase, endoglicanase e betaglicosidase, uso do mesmo, preparação de enzima, uso da mesma, vetor, célula hospedeira de fungo e cepa de escherichia coli, bem como método de preparação de um polipeptídeo e uso do mesmo
CN201110021502.9A CN102174492B (zh) 2005-12-22 2006-12-15 纤维素材料的处理和用于其中的酶
AU2006326963A AU2006326963B2 (en) 2005-12-22 2006-12-15 Treatment of cellulosic material and enzymes useful therein
RU2008123954/10A RU2458128C2 (ru) 2005-12-22 2006-12-15 Способ обработки целлюлозного материала и используемые в нем ферменты
PCT/FI2006/050558 WO2007071818A1 (en) 2005-12-22 2006-12-15 Treatment of cellulosic material and enzymes useful therein
CA2632502A CA2632502C (en) 2005-12-22 2006-12-15 Treatment of cellulosic material and enzymes useful therein
CN2006800528641A CN101384713B (zh) 2005-12-22 2006-12-15 纤维素材料的处理和用于其中的酶
ES06830936.8T ES2501944T3 (es) 2005-12-22 2006-12-15 Tratamiento de material celulósico y enzimas útiles en el mismo
EP06830936.8A EP1963498B1 (en) 2005-12-22 2006-12-15 Treatment of cellulosic material and enzymes useful therein
AP2008004509A AP2924A (en) 2005-12-22 2006-12-15 Treatment of cellulosic material and enzymes useful therein
EP10191152.7A EP2319920B1 (en) 2005-12-22 2006-12-15 Treatment of cellulosic material and enzymes useful therein
EP12151850.0A EP2453013B1 (en) 2005-12-22 2006-12-15 Treatment of cellulosic material and enzymes useful therein
CA2833029A CA2833029C (en) 2005-12-22 2006-12-15 Treatment of cellulosic material and enzymes useful therein
DK12151850.0T DK2453013T3 (en) 2005-12-22 2006-12-15 Treatment of cellulose materials and enzymes useful therefor
ES12151850.0T ES2582320T3 (es) 2005-12-22 2006-12-15 Tratamiento de material celulósico y enzimas útiles en el mismo
ARP060105762A AR058721A1 (es) 2005-12-22 2006-12-21 Triamiento de material celulosico y enzimas utiles en el mismo
TW095148542A TWI391489B (zh) 2005-12-22 2006-12-22 處理纖維素材料之方法及用於彼之酶類
ZA200804976A ZA200804976B (en) 2005-12-22 2008-06-06 Treatment of cellulosic material and enzymes useful there-in
US12/141,976 US20090042266A1 (en) 2005-12-22 2008-06-19 Treatment of cellulosic material and enzymes useful thererin
US12/917,603 US8409836B2 (en) 2005-12-22 2010-11-02 Treatment of cellulosic material and enzymes useful therein
US13/774,465 US9758777B2 (en) 2005-12-22 2013-02-22 Treatment of cellulosic material and enzymes useful therein
US14/045,236 US9593324B2 (en) 2005-12-22 2013-10-03 Treatment of cellulosic material and enzymes useful therein
US15/690,513 US20180044656A1 (en) 2005-12-22 2017-08-30 Treatment of Cellulosic Material and Enzymes Useful Therein

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US75325805P 2005-12-22 2005-12-22
FI20051318A FI120045B (sv) 2005-12-22 2005-12-22 Behandling av cellulosamaterial och däri användbara enzym
US75325805 2005-12-22
FI20051318 2005-12-22

Publications (3)

Publication Number Publication Date
FI20051318A0 FI20051318A0 (sv) 2005-12-22
FI20051318A FI20051318A (sv) 2006-12-28
FI120045B true FI120045B (sv) 2009-06-15

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FI20051318A FI120045B (sv) 2005-12-22 2005-12-22 Behandling av cellulosamaterial och däri användbara enzym

Country Status (14)

Country Link
US (5) US20090042266A1 (sv)
EP (4) EP2319920B1 (sv)
CN (2) CN101384713B (sv)
AP (1) AP2924A (sv)
AR (1) AR058721A1 (sv)
AU (1) AU2006326963B2 (sv)
BR (1) BRPI0620287B1 (sv)
CA (2) CA2833029C (sv)
DK (2) DK2453014T3 (sv)
ES (4) ES2501944T3 (sv)
FI (1) FI120045B (sv)
RU (1) RU2458128C2 (sv)
TW (1) TWI391489B (sv)
WO (1) WO2007071818A1 (sv)

Families Citing this family (203)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101522904A (zh) * 2006-01-27 2009-09-02 麻萨诸塞州大学 生产生物燃料和相关材料的系统和方法
EP2415803A3 (en) 2006-10-26 2012-10-31 Xyleco, Inc. Method of making a composite from biomass
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